Changes in the total amount of water-soluble antioxidants in the blood serum of Duroc boars depending on the duration of their fattening

2020 ◽  
Vol 1 (12) ◽  
pp. 112-118
Author(s):  
O. A. Voronina ◽  
◽  
A. A. Savina ◽  
N. S. Kolesnik ◽  
N. V. Bogolyubova ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Blood Serum ◽  
Water Soluble ◽  
Laboratory Studies ◽  
Blood Samples ◽  
Amperometric Method ◽  
Significant Difference ◽  
Total Antioxidant ◽  
First Time

The influence of fattening conditions on the digestive and metabolic processes in animals is actively studied to increase the production of animal food. The aim of the study was to estimate the total amount of water-soluble antioxidants in the blood serum of Duroc boars depending on the duration of fattening. Experiments were conducted on 102 animals to confirm the results of laboratory studies, as well as determination of the total antioxidant activity of pig blood serum by amperometric method. For the first time, a significant difference in antioxidant activity was found in blood samples of Duroc boars with the duration of their fattening from 81 to 101 days.

Amperometric detection of water-soluble antioxidants in chitosan-based feed additives and in sheep blood serum

2020 ◽  
Vol 1 (4) ◽  
pp. 68-74
Author(s):  
A. A. Savina ◽  
◽  
A. A. Volnin ◽  
N. V. Bogolyubova ◽  
O. A. Voronina ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Blood Serum ◽  
Amperometric Detection ◽  
Feed Additives ◽  
Water Soluble ◽  
High Protein ◽  
Feed Additive ◽  
Microbiological Synthesis ◽  
Sheep Blood ◽  
Amperometric Method

The effects of chitosan on various metabolic and digestive processes in the animal body are an important to study because of the production quality for animal nutrition, healthcare, etc. The major known changes in the biochemical parameters of the animal liquids (by chitosan usage as feed additive) are the following: ratio of volatile and fatty acids, content of fat, mineral composition, etc. The aim of this study was to evaluate the total amount of water-soluble antioxidants (TAWSA) of feed additives based on chitosan and high protein concentrate of microbiological synthesis and to assess the effect of these feed additives on TAWSA values of sheep blood serum by amperometric method. Firstly, the antioxidant activity of feed components based on chitosan or/and a high-protein microbiological synthesis concentrate was studied in this work. Second, the experiments are carried out on 6 fistula animals to confirm the results of laboratory studies. Third, the determination of the total antioxidant activity of sheep blood by using chitosan additives (as the feed components) by amperometric method was carried out. A particular difference in antioxidant activity of the control and experimental samples of the sheep blood was established for the first time.

scholarly journals Antioxidant Activity and Characterization of One New Polysaccharide Obtained fromPerigord Truffle(Tuber huidongense)

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Yong-Feng Chen ◽  
Wen-wen Jiang ◽  
Shi-qi Zhang ◽  
Jian-quan Kan ◽  
Yong Liang
Keyword(s):  
Molecular Structure ◽  
Antioxidant Activity ◽  
Structural Characteristics ◽  
Water Soluble ◽  
Separation And Purification ◽  
Edible Fungus ◽  
Main Components ◽  
First Time

As a medicinal and edible fungus parasitizing on the trees, Perigord Truffle (Tuber huidongense) is well known for its delicious taste, unique smell, and high medical value for healthcare. One new water-soluble nonstarch polysaccharide (PST-W with the yield of 0.41%) fromPerigord Truffle(Tuber huidongense) was purified and identified on structural characteristics for the first time. The characterizations of PST-W were studied on physicochemical properties, main components of monosaccharide(s), and molecular structure. The monosaccharide compositions of PST-W were studied and identified as glucan, only containing D-glucoses with the molecular structure of [→6)α-D-Glcp(1→6)  α-D-Glcp (1→]nby methylation analysis and NMR. In the determination of total reducing capacity, the reducing abilities of polysaccharide extracts could be listed as vitamin C > PST-W > crude polysaccharides-3 > crude polysaccharides-2 > crude polysaccharides-1. All of PST-W, crude polysaccharides-2, and crude polysaccharides-3 were relatively good scavenger for 1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl radicals with IC50of 2.81, 4.17, and 3.44 mg/mL, respectively. However,O2-∙clearing abilities of PST-W and crude polysaccharides were obviously weaker. The activities of total crude extract were the worst, indicating that the impurities might negatively affect the antioxidant activity. Thus, the separation and purification of polysaccharides were significant to increase the antioxidant activity in some degree.

Antioxidant status of blood in patients with vibration disease

2019 ◽  
pp. 978-982 ◽  
Author(s):  
N. N. Malyutina ◽  
A. F. Bolotova ◽  
R. B. Eremeev ◽  
A. Zh. Gilmanov ◽  
D. Yu. Sosnin
Keyword(s):  
Antioxidant Activity ◽  
Blood Serum ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Main Group ◽  
Biologically Active ◽  
Antioxidant Systems ◽  
Control Group ◽  
Vibration Disease ◽  
Total Antioxidant

Introduction. The overwhelming number of publications contains only data on the content of individual antioxidants, but not on the overall antioxidant activity of the blood in patients with vibration disease.The aim of the study was to determine the total antioxidant activity of blood serum in patients with vibration disease.Materials and methods. Th e main group consisted of 30 people diagnosed with “Vibration disease” of 1 degree (n=21) and 2 degrees (n=9). Th e control group consisted of 30 clinically healthy men, comparable in age with the main group (p=0.66). Th e total activity of antioxidant systems of blood plasma was evaluated photometrically using the test system “Total antioxidant status-Novo” (“Vector-best”, Russia).Results. The indicator of the total antioxidant status (TAS) was 1,038±0.232 mmol/l in the examined main group, against 1,456±0.225 mmol/l in the examined control group (p<0.000001). Th e coefficient of variation (CV) in patients with vibration disease was 22.35%, 1.45 times higher than in the control group (15.45%). In the main group there was a positive correlation between age and TAS (R=0.525), in the control group there was no such relationship (R=0.095). Th e degree of decrease depended on the severity of vibration disease.Conclusions. 1. The development of vibration disease is accompanied by a decrease in the antioxidant status of blood serum. 2. Th e degree of decrease in the antioxidant status of blood serum correlates with the severity of vibration disease. 3. Reduction of TAS can serve as a pathogenetic justification of the need to include drugs and/or biologically active additives with antioxidant activity in therapy

scholarly journals Chemical Composition of White Wines Produced from Different Grape Varieties and Wine Regions in Slovakia

2021 ◽  
Vol 11 (22) ◽  
pp. 11059
Author(s):  
Silvia Jakabová ◽  
Martina Fikselová ◽  
Andrea Mendelová ◽  
Michal Ševčík ◽  
Imrich Jakab ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Mean Value ◽  
Total Phenolic ◽  
White Wines ◽  
Tartaric Acids ◽  
Total Antioxidant ◽  
Total Polyphenolic Content ◽  
Wine Region ◽  
Grape Varieties

In this work, chemical parameters such as sugar (glucose and fructose) content, organic acid (total acids, malic and tartaric acids), total phenolic content and the antioxidant activity of 12 white wines (chardonnay, pinot blanc and pinot gris) from various wine regions in Slovakia were studied in order to identify differences among the varieties and wine-growing regions. The wine samples were examined by Fourier-transform infrared spectroscopy (FTIR) and UV-VIS spectrophotometry (for determination of total polyphenolic content (TPC) and total antioxidant activity (TAA)) methods. Content of alcohol ranged between 11.50% and 13.80% with the mean value 12.52%. Mean content of total acids varied between 4.63 ± 0.09 and 6.63 ± 0.05 g.L−1, tartaric acid varied between 1.62 ± 0.09 and 2.93 ± 0.03 g L−1, malic acid was found in the concentrations ranged from 0.07 ± 0.05 and 2.50 ± 0.08 g L−1 and lactic acid was present between 1.53 and 0.01 g L−1. The content of fructose was, in general, higher in the samples from the Južnoslovenská and Nitrianska wine regions and glucose was higher in the Malokarpatská wine region. Chardonnay wines showed the highest content of total polyphenols and the antioxidant activity in the samples ranged from 51.06 ± 027 to 72.53 ± 0.35% inhibition of DPPH. The PCA analysis based on chemical descriptors distinguished the Nitrianska and Stredoslovenská wine regions. According to similarities among the wine samples, four main classes were formed by cluster analysis.

Simultaneous determination of Rivaroxaban and TAK-438 in rat plasma by LC–MS/MS: application to pharmacokinetic interaction study

Bioanalysis ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 11-22 ◽  
Author(s):  
Libin Wang ◽  
Shouchang Gai ◽  
Xiaorui Zhang ◽  
Xiaohui Xu ◽  
Nan Gou ◽  
...  
Keyword(s):  
Drug Interactions ◽  
Internal Standard ◽  
Pharmacokinetic Interaction ◽  
Rat Plasma ◽  
Pharmacokinetic Parameters ◽  
Interaction Study ◽  
Significant Difference ◽  
Us Fda ◽  
First Time

Aim: A sensitive and reliable LC–MS/MS method has been established and validated to the quantitation of rivaroxaban (RIV) and TAK-438 in rat plasma using carbamazepine as internal standard. Results: The procedure of method validation was conducted according to the guidelines of EMA and US FDA. At the same time, the method was applied to pharmacokinetic interactions study between RIV and TAK-438 for the first time. When RIV and TAK-438 co-administration to rats, main pharmacokinetic parameters of TAK-438 like AUC(0-t), AUC(0-∞) and Cmax had statistically significant increase. The main pharmacokinetic parameters of RIV have no statistically significant difference (p > 0.05) when co-administered except for t1/2 (p < 0.01). Conclusion: The results indicated that drug–drug interactions occurred between RIV and TAK-438 when co-administered to rats.

Spectrofluorimetric Determination of Some Water-Soluble Vitamins

2011 ◽  
Vol 94 (6) ◽  
pp. 1758-1769 ◽  
Author(s):  
Abdel-Maaboud I Mohamed ◽  
Horria A Mohamed ◽  
Niveen M Abdel-Latif ◽  
Marwa R Mohamed
Keyword(s):  
Water Soluble ◽  
Good Precision ◽  
Linear Calibration ◽  
Reaction Conditions ◽  
Pharmaceutical Dosage Forms ◽  
Accuracy And Precision ◽  
Significant Difference ◽  
Spectrofluorimetric Determination ◽  
Comparison Of The Results

Abstract Two simple and sensitive spectrofluorimetric methods were developed for determination of three water-soluble vitamins (B1, B2, and B6) in mixtures in the presence of cyanocobalamin. The first one was for thiamine determination, which depends on the oxidation of thiamine HCl to thiochrome by iodine in an alkaline medium. The method was applied accurately to determine thiamine in binary, ternary, and quaternary mixtures with pyridoxine HCl, riboflavin, and cyanocobalamin without interference. In the second method, riboflavin and pyridoxine HCl were determined fluorimetrically in acetate buffer, pH 6. The three water-soluble vitamins (B1, B2, and B6) were determined spectrofluorimetrically in binary, ternary, and quaternary mixtures in the presence of cyanocobalamin. All variables were studied in order to optimize the reaction conditions. Linear relationship was obeyed for all studied vitamins by the proposed methods at their corresponding λexc or λem. The linear calibration curves were obtained from 10 to 500 ng/mL; the correlation ranged from 0.9991 to 0.9999. The suggested procedures were applied to the analysis of the investigated vitamins in their laboratory-prepared mixtures and pharmaceutical dosage forms from different manufacturers. The RSD range was 0.46–1.02%, which indicates good precision. No interference was observed from common pharmaceutical additives. Good recoveries (97.6 ± 0.7–101.2 ± 0.8%) were obtained. Statistical comparison of the results with reported methods shows excellent agreement and indicates no significant difference in accuracy and precision.

scholarly journals Increasing Anthocyanin Content in Tomato Fruit for Improved Antioxidant Activity

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 869D-869
Author(s):  
Peter J. Mes* ◽  
James R. Myers
Keyword(s):  
Antioxidant Activity ◽  
Tomato Fruit ◽  
Introgression Line ◽  
Water Soluble ◽  
Carotenoid Content ◽  
Anthocyanin Content ◽  
Total Antioxidant Activity ◽  
Total Antioxidant

Tomato lines carrying the genes Aft, atv, Abg, hp-1, and an as yet undetermined gene from the introgression line LA2099 have been combined to produce fruit with elevated anthocyanin content. The antioxidant activity of juice made from anthocyanin-expressing tomatoes was compared to juices made from tomatoes with varied carotenoid content. The contribution of anthocyanin to the total antioxidant activity of the whole fruit in current material is small, but with potential for significant improvement. The increase in flavonoids in the elevated anthocyanin lines has increased water-soluble antioxidant activity of the fruit in vitro.

scholarly journals Ultrasonic estimation of gestation age in goats via placentomes diameter

2017 ◽  
Vol 40 (2) ◽  
pp. 100-106
Author(s):  
Yaseen Mahmood Rasheed
Keyword(s):  
Linear Array ◽  
Early Recognition ◽  
Array Transducer ◽  
Gestation Age ◽  
Starting Point ◽  
Linear Probe ◽  
Significant Difference ◽  
First Time ◽  
The Relationship

     The aim of the present study is to establish the relationship between gestational age and placentome measurement via real-time ultrasound. This study is conducted in the college of Veterinary Medicine at Diyala University. Seventeen multiparous does were synchronized for estrus and naturally served to obtain pregnancy and subsequently scanned using both transducers. Ultrasonic (U.S) examination was performed transabdominally (TA) with sector-probe (5MHz) and transrectally (TR) with linear-probe (7.5MHz), in 10 day interval done started from day 35-135 post mating. The placentome was observed for the first time on day 35 by using TR ultrasonic examination (7.5MHz) linear transducer, as echogenic densities on the surface of endometrium. The results showed a significant increased (P≤0.05) in placentomes growth with gestation age. The placentome diameter (PD) reached maximized size around days 126 (39.6±2.37mm). Also, the results indicated significant difference between placentome diameter (PD) size in single and twin-pregnant does at (P≤0.05). The average of PD size in the single and twin-pregnant does was, respectively, 7.5±0.41mm and 9.2±0.74 mm at the day 35 and 45 of gestation. The maximal size of PD was 39.2±2.50mm in singleton-bearing does during 116-125 day and 41.0±2.19 mm in twin-bearing does during 126-135 day of gestation. In conclusion, the determination of gestation age according to placentome measurement was not reliable after day 90. Also, the use of 7.5 MHz linear array transducer for TR examination was found to be efficient, for early recognition of placentomes starting point from 35 days of gestation, as well as, the larger placentomes are expected in twins-bearing does. 

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