scholarly journals Hasil diagnostik Mycobacterium tuberculosis pada penderita batuk ≥2 minggu dengan pewarnaan Ziehl-Neelsen di Puskesmas Bailang dan Puskesmas Bengkol Manado

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Stephanie O. Wilar ◽  
John Porotu'o ◽  
Olivia Waworuntu
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Positive Result ◽  
Descriptive Study ◽  
Acid Fast Bacillus ◽  
Tuberculosis Patients ◽  
Tuberculosis Transmission ◽  
Source Of Infection ◽  
Keywords Tuberculosis ◽  
Infection Source ◽  
Global Health Problem

Abstract: Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis. Transmission occurs by air droplet infection. Source of infection is sputum of pulmonary tuberculosis patients. To date, tuberculosis is still a global health problem. In Indonesia the number of tuberculosis cases increases annualy. Most tuberculosis patients complain of cough ≥2 weeks. This study was aimed to determine the diagnostic results of M. tuberculosis in the sputum of patients cough ≥2 weeks with Ziehl-Neelsen staining at Puskesmas Bailang and Bengkol. This was a descriptive study. Subjects were patients with complaints of cough ≥2 weeks at Puskesmas Bailang and Bengkol. The results showed that there were 25 patients with cough ≥2 weeks that came for treatment consisted of 12 patients at Puskesmas Bailang and 13 patients at Puskesmas Bengkol. Most of them were males and aged 36-55 years. There was 1 patient (8.33%) that had positive result of acid-fast bacillus at Puskesmas Bailang however, at Puskesmas Bengkol none had positive result. Keywords: tuberculosis, cough more than two weeks, BTA examination. Abstrak: Tuberkulosis (TB) adalah penyakit infeksi yang disebabkan oleh Mycobacterium tuberculosis. Penularan terjadi melalui udara dari droplet infeksi. Sumber infeksi ialah penderita tuberkulosis paru yang membatukkan dahaknya. Hingga saat ini tuberkulosis masih merupakan masalah kesehatan dunia. Di Indonesia kasus tuberkulosis menunjukkan peningkatan dari tahun ke tahun. Diantara sekian banyak kasus tuberkulosis, pasien datang berobat dengan keluhan batuk ≥2 minggu. Penelitian ini bertujuan untuk mengetahui hasil diagnostik M. tuberculosis dari sputum penderita batuk ≥2 minggu dengan pewarnaan Ziehl- Neelsen di Puskesmas Bailang dan Bengkol. Jenis penelitian ialah deskriptif. Subjek penelitian ialah pasien dengan keluhan batuk ≥2 minggu di Puskesmas Bailang dan Bengkol. Hasil penelitian mendapatkan sebanyak 25 pasien dengan batuk ≥2 minggu yang datang berobat di Puskesmas Bailang dan Bengkol, terdiri dari 12 pasien dari Bailang dan 13 pasien dari Bengkol. Keluhan batuk ≥2 minggu lebih banyak ditemukan pada laki-laki, dan usia 36-55 tahun. Hasil pemeriksaan BTA di Puskesmas Bailang ditemukan 1 pasien (8,33%) positif sedangkan di Puskesmas Bengkol tidak ditemukan BTA positif.Kata kunci: tuberkulosis, batuk lebih dari dua minggu, pemeriksaan BTA.

scholarly journals Value of Examining Three Acid-Fast Bacillus Sputum Smears for Removal of Patients Suspected of Having Tuberculosis from the “Airborne Precautions” Category

2000 ◽  
Vol 38 (11) ◽  
pp. 4285-4287 ◽  
Author(s):  
David W. Craft ◽  
Melissa C. Jones ◽  
Cherie N. Blanchet ◽  
Roy L. Hopfer
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Positive Result ◽  
Potential Risk ◽  
Acid Fast Bacillus ◽  
Additional Patient ◽  
Positive Finding ◽  
Tuberculosis Transmission ◽  
The Third

We examined the potential risk of tuberculosis transmission if we modified our policy for release of patients from the “airborne precautions” category from three negative acid-fast bacillus (AFB) smears to two, or even one. Over a 4-year period, respiratory cultures from 42 patients grew Mycobacterium tuberculosis. Of these, 36 patients (81%) had a positive AFB smear result on the first submitted specimen. One additional patient (2%) had a first smear-positive finding on the second submitted specimen, and no patients had a first smear-positive result on the third submitted specimen. Respiratory cultures from five patients (12%) grew M. tuberculosis without ever having a positive AFB smear result. These data indicate that in our institution, reducing the number of negative smears required before removal of patients from the airborne precautions category would pose little, if any, increase in the risk of spreading tuberculosis.

scholarly journals Gambaran Pasien Tuberkulosis Paru Usia Produktif di RSUP Prof. Dr. R. D. Kandou Manado Periode Tahun 2014-2015

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
Johanes F. N. Kesek ◽  
Cerelia E. C. Sugeng ◽  
Efata B. I. Polii
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Observational Study ◽  
Pulmonary Tuberculosis ◽  
Medical Records ◽  
Acid Fast Bacillus ◽  
Age Group ◽  
Tuberculosis Patients ◽  
Pulmonary Tb ◽  
Using Data ◽  
Global Health Problem

Abstract: Pulmonary tuberculosis (pulmonary TB) is caused by Mycobacterium tuberculosis, an acid-fast bacillus (AFB). This disease is characterized by pulmonary infiltrates and caseous granuloma formation, fibrosis, as well as cavities. To date, this disease is still a global health problem. The highest incidence of pulmonary TB is usually in productive age between 15 and 44 years. This study was aimed to obtain the profile of pulmonary tuberculosis patients in productive age at RSUP Prof. Dr. R. D. Kandou Manado during years 2014 to 2015. This was a descriptive observational study using data of medical records. The result showed that there were 178 pulmonary TB patients in the age group of 15-44 years. Based on sex, there were 94 males (52.8%) and 84 females (47.2%). TB patients with positive acid-fast bacilli (AFB) in sputum were found in 117 patients (65.7%) and those with negative AFB in 61 patients (34.3%). In conclusion, pulmonary TB patients at productive age (15-44 years) were predominant in males with positive sputum of Mycobacterium tuberculosis.Keyword: pulmonary tuberculosis, productive age Abstrak: Tuberkulosis (TB) paru adalah infeksi paru yang disebabkan oleh Mycobacterium tuberculosis, yaitu sejenis basil tahan asam (BTA). Penyakit ini ditandai oleh adanya infiltrat paru dan pembentukan granuloma kaseosa, fibrosis, dan kavitas. Penyakit TB paru masih merupakan masalah global dengan insidensi tertinggi biasanya mengenai usia produktif, antara 15–44 tahun. Penelitian ini bertujuan untuk mengetahui gambaran pasien tuberkulosis paru usia produktif di RSUP Prof. Dr. R. D. Kandou Manado periode tahun 2014-2015. Jenis penelitian ialah deskriptif observasional menggunakan data rekam medis. Hasil penelitian mendapatkan 178 pasien TB paru pada golongan usia 15-44 tahun. Berdasarkan jenis kelamin didapatkan 94 orang laki-laki (52,8%) dan 84 orang perempuan (47,2%). Pasien TB dengan hasil pemeriksaan sputum BTA (+) sebanyak 117 orang (65,7%) dan pasien dengan hasil pemeriksaan sputum BTA (-) sebanyak 61 orang (34,3%). Simpulan penelitian ini ialah penderita TB paru pada usia produktif (15-44 tahun) lebih sering didapatkan pada jenis kelamin laki-laki dan dengan hasil pemeriksaan sputum BTA (+).Kata kunci: tuberkulosis paru, usia produktif

scholarly journals Hasil Diagnostik Mycobacterium tuberculosis pada Pasien Batuk 2 Minggu dengan Pewarnaan Ziehl Neelsen di Poliklinik Interna RSU Pancaran Kasih Manado

2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Natasha M. Pomandia ◽  
Olivia A. Waworuntu ◽  
Heriyannis Homenta
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Positive Result ◽  
Gold Standard ◽  
Descriptive Study ◽  
Negative Results ◽  
Common Complaint ◽  
Contagious Disease ◽  
Tuberculosis Diagnosis

Abstract: Tuberculosis (TB) is a contagious disease caused by various strains of mycobacteria, commonly Mycobacterium tuberculosis. This disease can occur in children as well as in adults. The most common complaint in TB patients is cough. The gold standard of tuberculosis diagnosis is the culture of TB bacteria. However, Ziehl-Neelsen staining is the most frequently used method. It gives positive result if there are more than 103 bacteria per ml sputum. This study was aimed to find out the existence of M. tuberculosis in the sputum of patients coughed ≥2 weeks at the Internal Polyclinic of Pancaran Kasih Hospital Manado by using Ziehl-Neelsen staining. This was a descriptive study using 30 patients as samples. Of 30 samples, all (100%) had negative results of acid resistant bacili. Conclusion: In this study, all patients with cough ≥2 weeks in the Internal Polyclinic of Pancaran Kasih Hospital Manado had negative results of M. Tuberculosis based on Ziehl-Neelsen staining.Keywords: tuberculosis, cough ≥2 weeks Abstrak: Tuberkulosis (TB) ialah penyakit menular yang disebabkan oleh berbagai strain mikobakteria, umumnya Mycobacterium tuberculosis. Penyakit ini dapat terjadi pada anak atau orang dewasa. Keluhan yang sering ditemukan pada pasien TB ialah batuk. Dalam menegakkan diagnosis TB, baku emas pemeriksaan ialah kultur kuman TB. Selain kultur dapat digunakan metode pewarnaan basil tahan asam (BTA) Ziehl-Neelsen. Metode ini yang paling sering digunakan dan memberikan hasil positif bila terdapat lebih dari 103 kuman/ml sputum. Penelitian bertujuan untuk mengetahui ada tidaknya bakteri M. tuberculosis dengan menggunakan pewarnaan Ziehl-Neelsen pada pasien dengan batuk ≥2 minggu di Poliklinik Interna RSU Pancaran Kasih Manado. Jenis penelitian ialah deskriptif dengan mengunakan sebanyak 30 sampel. Hasil penelitian mendapatkan pada seluruh sampel (100%) tidak ditemukan BTA positif. Simpulan: Pada pasien dengan batuk ≥2 minggu di Poliklinik Interna RSU Pancaran Kasih Manado tidak ditemukan BTA positif berdasarkan pewarnaan Ziehl-Neelsen.Kata kunci: tuberkulosis (TBC), batuk ≥ 2 minggu

scholarly journals Hasil diagnostik Mycobacterium tuberculosis pada penderita batuk ≥2 minggu dengan pewarnaan Ziehl-Neelsen di Puskesmas Ranomuut dan Puskesmas Kombos Manado

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Ramalia P. Mohamad ◽  
John Porotu'o ◽  
Heriyannis Homenta
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Positive Result ◽  
Staining Method ◽  
Descriptive Study ◽  
Health Centers ◽  
Common Symptom ◽  
Acid Fast Bacilli ◽  
Primary Health ◽  
The Common

Abstract: The common symptom of lung disease is cough which is usually recovered after 2-3 weeks. However, if the cough persists continually then it should be paid attention seriously ith detect Mycobacterium tuberculosis in the sputum of patients with cough ≥2 weeks at Puskesmas Ranomuut and Puskesmas Kombos (primary health centers) in Manado by using the Ziehl-Neelsen staining method. This was a descriptive study using total sampling from August to November 2016. The results showed that there were 25 patients with cough ≥2 weeks; 7 patients at Puskesmas Ranomuut and 18 patients at Puskesmas Kombos. The majority of patients were male (68%) and aged 15-34 years old (44%). The Ziehl-Neelsen staining of patients’ sputum revealed that at Puskesmas Kombos there were 2 patients (11,1%) with positive acid-fast bacilli meanwhile at Puskesmas Ranomuut there was no positive result. Conclusion: Acid-fast bacilli were detected in 11.1% patients with cough ≥2 weeks at Puskesmas Kombos only. Keywords: Mycobacterium tuberculosis, pulmonary tuberculosis, Ziehl-Neelsen Abstrak: Gejala penyakit paru yang paling sering ditemukan ialah batuk. Batuk biasanya akan membaik dalam 2-3 minggu namun bila batuk terjadi terus-menerus perlu diselidiki lebih lanjut karena merupakan gejala utama dari penyakit tuberkulosis paru. Penelitian ini bertujuan untuk mendeteksi adanya kuman Mycobacterium tuberkulosis dalam dahak penderita batuk ≥2 minggu di Puskesmas Ranomuut dan Puskesmas Kombos dengan pemeriksaan mikrobiologi menggunakan teknik pewarnaan Ziehl-Neelsen. Jenis penelitian ialah deskriptif. Pengambilan sampel menggunakan cara total sampling dalam kurun waktu Agustus-November 2016. Hasil penelitian mendapatkan 25 pasien batuk ≥2 minggu terdiri dari 7 pasien di Puskesmas Ranomuut dan 18 pasien di Puskesmas Kombos. Jumlah pasien laki-laki (68%) lebih banyak dibandingkan perempuan (32%) dengan usia terbanyak 15-34 tahun (44%). Pada pemeriksaan BTA di Puskesmas Ranomuut tidak ditemukan pasien dengan BTA positif sedangkan di Puskesmas Kombos terdapat 2 pasien dengan BTA positif (11,1%). Simpulan: Kuman BTA positif dalam sputum penderita batuk ≥2 minggu sebanyak 2 orang (11,1%) hanya di Puskesmas Kombos.Kata kunci: Mycobacterium tuberculosis, tuberkulosis paru, Ziehl-Neelsen

scholarly journals Gambaran bilirubin dan urobilinogen urin pada pasien tuberkulosis paru dewasa di RSUP Prof. Dr. R. D. Kandou Manado

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Faleriano Makay ◽  
Glady I. Rambert ◽  
Mayer F. Wowor
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Normal Level ◽  
Random Sampling ◽  
Descriptive Study ◽  
Tuberculosis Patient ◽  
Inclusion Criteria ◽  
Tuberculosis Patients ◽  
Observational Descriptive Study ◽  
Hepatotoxic Drug

Abstract: Tuberculosis is an infectious disease caused by the bacillus Mycobacterium tuberculosis. Antituberculosis drugs prescribed to TB patients is hepatotoxic drug. Liver damage caused by side effects of the drugs will cause an alteration in urinary bilirubin and urobilinogen level. This study was aimed to obtain the profile of urinary bilirubin and urobilinogen in adult pulmonary tuberculosis patients at Prof. Dr. R. D. Kandou Hospital Manado from October to November 2016. This was an observational descriptive study. Samples obtained by using random sampling urine from all pulmonary tuberculosis patient that met the inclusion criteria. The results showed that according to urinary bilirubin and urobilinogen examination in 30 patients, most of them were in normal level. Only 6 out of 30 patients has bilirubinuria in this urinary bilirubin examination. In urinary urobilinogen examination, all results is in normal level. Conclusion: Urinary bilirubin and urobilinogen examination in this research was normal in general, bilirubinemia was found only in some patients.Keywords: pulmonary tuberculosis, urinary bilirubin, urinary urobilinogen Abstrak: Tuberkulosis (TB) merupakan penyakit menular yang disebabkan oleh basil Mycobacterium tuberculosis. Obat antituberkulosis yang diberikan pada pasien TB bersifat hepatotoksik. Kerusakan hepar yang disebabkan oleh efek samping obat tersebut akan menyebabkan perubahan pada kadar bilirubin dan urobilinogen urin. Tujuan penelitian untuk mengetahui gambaran bilirubin dan urobilinogen urin pada pasien tuberkulosis paru dewasa di RSUP Prof. Dr. R. D. Kandou Manado. Jenis penelitian ialah deskriptif observasional, untuk mendapatkan data tentang bilirubin dan urobilinogen urin pada pasien tuberkulosis paru dewasa di RSUP. Prof. Dr. R. D. Kandou Manado pada Oktober-November 2016. Sampel penelitian adalah sampel urin sewaktu dari semua pasien tuberkulosis paru yang memenuhi kriteria inklusi. Hasil penelitian memperlihatkan berdasarkan pemeriksaan bilirubin dan urobilinogen urin pada 30 pasien, sebagian besar kadarnya normal. Hanya 6 dari 30 pasien yang mengalami bilirubinuria pada pemeriksaan bilirubin urin. Pada pemeriksaan urobilinogen urin semua hasil dalam batas normal. Simpulan: Pemeriksaan bilirubin dan urobilinogen urin pada umumnya normal, hanya beberapa pasien yang mengalami bilirubinuria.Kata kunci: tuberkulosis paru, bilirubin urin, urobilinogen urin

scholarly journals Gambaran eritrosit urin pada pasien tuberkulosis paru dewasa di RSUP Prof. Dr. R. D. Kandou Manado

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Gracia S. Sinaga ◽  
Glady I. Rambert ◽  
Mayer F. Wowor
Keyword(s):  
Infectious Disease ◽  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Observational Research ◽  
Inclusion Criteria ◽  
High Power Field ◽  
Tuberculosis Patients ◽  
Duration Of Therapy ◽  
Normal Urine ◽  
Global Health Problem

Abstract: Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. Tuberculosis remains a major global health problem and ranks as the second leading cause of death from an infectious disease worldwide. Anti-tuberculosis drugs, such as streptomycin and rifampicin are nephrotoxic. If the kidney function decreased, especially the glomerulus there can be found blood cells in the urine. A small number of erythrocytes may be found in normal urine, about 0-2 cells per HPF (High Power Field). But more than three erythrocytes per HPF is generally considered hematuria. This study aims to describe about how the urine erythrocytes in adult pulmonary tuberculosis patients at RSUP Prof. Dr. R. D. Kandou Manado. Research design used is descriptive observational research. Samples were urine samples of all pulmonary tuberculosis patients that suit to inclusion criteria. This study was conducted from October to November 2016. The results of chemical urinalysis of urine erythrocytes are negative in 20 patients and positive in 10 patients, while the results of microscopic urinalysis of urine erythrocytes are normal in 26 patients and hematuria in 4 patients. Conclusion: Hematuria mostly found in males, age 56-65 years old, default tuberculosis type, the first category of anti-tuberculosis drugs, the duration of therapy about 3-4 months, and in patients with comorbid disease.Keywords: pulmonary tuberculosis, urinalysis, hematuria Abstrak: Tuberkulosis merupakan penyakit infeksi yang disebabkan oleh Mycobacterium tuberculosis. Tuberkulosis masih merupakan salah satu masalah kesehatan masyarakat di dunia dan menduduki peringkat kedua penyebab kematian oleh penyakit infeksi di dunia. Obat anti-tuberkulosis seperti streptomisin dan rifampisin memiliki efek nefrotoksik. Apabila fungsi ginjal terutama glomerulus telah rusak maka dapat ditemukan adanya eritrosit dalam urin. Pada urin normal terdapat eritrosit sekitar 0-2 sel/LPB. Jika ditemukan 3 sel/LPB atau lebih, maka disebut hematuria. Penelitian ini bertujuan untuk mengetahui gambaran eritrosit urin pada pasien tuberkulosis paru dewasa di RSUP Prof. Dr. R.D. Kandou Manado. Desain penelitian yang digunakan adalah deskriptif observasional. Sampel penelitian adalah sampel urin sewaktu dari semua pasien tuberkulosis paru yang memenuhi kriteria inklusi. Penelitian dilakukan sejak Oktober-November 2016. Dari hasil pemeriksaan eritrosit urin secara kimia didapatkan hasil negatif pada 20 pasien dan hasil positif pada 10 pasien, sedangkan pemeriksaan mikroskopis eritrosit urin ditemukan hasil normal pada 26 pasien dan hasil hematuria pada 4 pasien. Simpulan: Hematuria didapatkan lebih banyak pada pasien laki-laki, pada kelompok usia 56-65 tahun, pada jenis kasus putus obat, pada jenis pengobatan kategori 1, pada lama pengobatan 3-4 bulan, dan pada pasien dengan penyakit penyerta. Kata kunci: tuberkulosis paru, urinalisis, hematuria

scholarly journals Comparison of the Performance of Urinary Mycobacterium tuberculosis Antigens Cocktail (ESAT6, CFP10, and MPT64) with Culture and Microscopy in Pulmonary Tuberculosis Patients

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
Dewi Kartika Turbawaty ◽  
Adhi Kristianto Sugianli ◽  
Arto Yuwono Soeroto ◽  
Budi Setiabudiawan ◽  
Ida Parwati
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Screening Test ◽  
Causes Of Death ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Tuberculosis Patients ◽  
Sensitivity Specificity ◽  
Global Health Problem

Pulmonary tuberculosis (TB) is a major global health problem and is one of the top 10 causes of death worldwide. Our study aimed to evaluate the performance of urinary Mycobacterium tuberculosis (Mtb) antigens cocktail (ESAT6, CFP10, and MPT64) compared with culture and microscopy. This descriptive cross-sectional study was conducted in Dr. Hasan Sadikin General Hospital, Bandung, from January 2014 to October 2016. A total of 141 pulmonary tuberculosis patients were included. Sputum samples were examined for acid-fast bacilli (ZN stain) and mycobacterial culture (LJ); the Mtb antigens cocktail was examined in the urine sample. The positivity rate of TB detection from the three methods was as follows: AFB 52/141 (36.9%), culture 50/141 (35.5%), and urinary Mtb antigens cocktail 95/141 (67.4%). Sensitivity, specificity, PPV, and NPV of urinary Mtb antigens cocktail were 68.2%, 33%, 31.6%, and 69.6%, respectively. Validity of combination of both methods with culture as a gold standard yielded sensitivity, specificity, PPV, and NPV of 90%, 28.6%, 40.9%, and 83.8%, respectively. Combination of urinary Mtb antigens cocktail with AFB as a screening test gives a good sensitivity, although the specificity is reduced. Urinary Mtb antigens cocktail can be used as screening test for pulmonary tuberculosis.

scholarly journals Spoligotyping, Resistensi Mycobacterium tuberculosis terhadap obat TB dan Faktor Risiko Penularan Tuberkulosis Paru di Kota Jayapura

2019 ◽  
Vol 47 (1) ◽  
pp. 1-12
Author(s):  
Hana Krismawati ◽  
Antonius Oktavian ◽  
Lydia Chaidir ◽  
Evy Iriani Natalia ◽  
Melda Suebu ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Tuberculosis Patients ◽  
Tuberculosis Transmission ◽  
Mycobacterium Growth Indicator Tube ◽  
Indicator Tube ◽  
Solid Media ◽  
Mdr Tb ◽  
Close Contacts ◽  
Growth Indicator

Identification of the Mycobacterium tuberculosis strain found in pulmonary tuberculosis patients in Jayapura using Spolygotyping was carried out Patients with pulmonary tuberculosis are taken as an index case. Sputum samples were taken to be diagnosed with Acid Fast Staining and to culture M. tuberculosis bacteria using Lowensten Jansen solid media, Ogawa solid media and Mycobacterium Growth Indicator Tube liquid media. The patient's home visit was conducted to determine the environmental conditions of the patient's residence, interview the close contacts and test the bacterial infection M. tuberculosis in close contact cases using the Mantoux test. A thorax radiology of close contacts was carried out in the hospital's radiology unit. The results of spolygotyping showed 8%sputum samples M. tuberculosis of Beijing strain, while the rest are M. tuberculosis non Beijing strains. Among the 46 of Non-Beijing strains, the type U (likely S) is most transmitted by tuberculosis patients to their close contacts, which is as much as 6%. This study confirms the factors that influence tuberculosis transmission are the sleeping location of close contacts with the patients, the frequency of meetings that occur at least once or more in a day, density of house occupants and the presence of clinical symptoms of TB. Drugs Resistance Susceptibility test results showed that 12 patients were resistance to TB drugs and one of them was MDR TB from strain LAMP. Key words : tuberculosis transmission, Spolygotyping, tuberculosis, Jayapura, drugs resistance   Abstrak Identifikasi galur Mycobacterium tuberculosis yang ditemukan pada penderita tuberculosis paru di Jayapura telah dilakukan dengan menggunakan Spoligotyping Penderita tuberkulosis paru diambil sebagai kasus indeks. Sampel sputum juga diambil untuk didiagnosis dengan pengecatan Basil Tahan Asam dan kultur bakteri M.tuberculosis yang dilakukan pada media padat Lowensten Jansen, media padat Ogawa serta media cair Mycobacterium Growth Indicator Tube. Ekstraksi DNA dilakukan pada koloni Mycobacterium tuberculosis untuk dilakukan analisis spoligotyping. Kunjungan rumah pasien dilakukan untuk mengetahui kondisi lingkungan tempat tinggal pasien, mewawancara kontak kasus dan melakukan skrining infeksi bakteri M.tuberkulosis pada kasus kontak menggunakan tes Mantoux. Foto dada pada kontak kasus selanjutnya dilakukan di unit radiologi rumah sakit. Hasil spoligotyping menunjukkan 8% (4/50) sampel sputum penderita tuberkulosis adalah M.tuberculosis galur Beijing, sedangkan sisanya adalah M.tuberculosis galur Non Beijing. Dari 46 galur Non-Beijing, tipe U (likely S) paling banyak ditransmisikan oleh penderita tuberculosis pada kontaknya yaitu sebanyak 6% (3/50). Penelitian ini mengkonfirmasi faktor-faktor yang mempengaruhi transmisi tuberkulosis meliputi lokasi tidur kontak kasus yang serumah dengan penderita, frekuensi pertemuan yang terjadi sedikitnya sekali atau lebih dalam sehari, kondisi rumah padat penghuni dan adanya gejala klinis TB. Hasil uji resistensi menunjukkan 12 pasien mengalami resistensi terhadap OAT dan 1 diantaranya adalah MDR TB dari strain LAMP. Kata kunci : Transmisi, Splygotyping, Mycobacterium tuberculosis, tuberkulosis, Jayapura, resistensi

scholarly journals Hasil diagnostik Mycobacterium tuberculosis pada penderita batuk ≥2 minggu dengan pewarnaan Ziehl-Neelsen di Puskesmas Tuminting dan Puskesmas Tongkaina Manado

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Rizca J. Domu ◽  
John Porotu'o ◽  
Olivia A. Waworuntu
Keyword(s):  
Public Health ◽  
Infectious Disease ◽  
Mycobacterium Tuberculosis ◽  
Health Service ◽  
Public Health Service ◽  
Descriptive Study ◽  
Cross Sectional ◽  
Primary Health ◽  
Keywords Tuberculosis ◽  
Cross Sectional Design

Abstract: Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis (M. tuberculosis). In Indonesia tuberculosis cases has been increasing from year to year. The examination of sputum by using Ziehl-Neelsen staining must be more improved for public health service. This study aimed to determine the profile of Mycobacterium tuberculosis (acid-fast bacteria) among patients with coughing ≥2 weeks at Tuminting and Tongkaina Primary Health Cares (PHCs) by using Ziehl-Neelsen staining. This was a descriptive study with a cross sectional design. Subjects were patients with complaints of cough ≥2 weeks Tuminting and Tongkaina PHC. The results showed that there were 30 cases of coughing ≥2 weeks as follows: 28 cases at Tuminting PHC and 2 cases at Tongkaina PHC. The examination of acid-fast bacteria from the 30 cases of three PHCs showed that 2 case (7.1%) had acid-fast bacteria (++) and (+++) in Tuminting PHC and in Tongkaina PHC not found smear-positive . Conclusion: In this study, there was two case (7,1%) with positive Mycobacterium tuberculosis. Keywords: tuberculosis, cough more than two weeks, BTA examination Abstrak: Tuberkulosis adalah suatu penyakit infeksi yang disebabkan oleh Mycobacterium tuberculosis (M. tuberculosis). Di Indonesia kasus tuberculosis menunjukkan peningkatan dari tahun ke tahun. Pemeriksaan sputum dengan pewarnaan Ziehl-Neelsen harus lebih ditingkatkan pada pelayanan kesehatan masyarakat. Penelitian ini bertujuan untuk mendapatkan gambaran Mycobacterium tuberculosis (basil tahan asam) dengan pewarnaan Ziehl-Neelsen pada pasien batuk ≥2 minggu di Puskesmas Tuminting dan Tongkaina Manado. Jenis penelitian ini deskriptif dengan desain potong lintang. Subjek penelitian adalah pasien dengan keluhan batuk ≥2 minggu di Puskesmas Tuminting dan Tongkaina. Jumlah penderita batuk ≥2 minggu yang datang berobat di Puskesmas Tuminting dan Tongkaina sebanyak 30 pasien, yang terdiri dari 28 pasien dari Puskesmas Tuminting dan 2 pasien dari Puskesmas Tongkaina. Pada pemeriksaan (BTA) di didapatkan BTA (++) dan (+++) 7.1% sedangkan BTA (-) 92,9% di Puskesmas Tuminting. Puskesmas Tongkaina tidak ditemukan BTA positif. Simpulan: Pada penelitian ini didapatkan 2 kasus (7.1%) Mycobacterium tuberculosis positif.Kata kunci: tuberkulosis, batuk lebih dari dua minggu, pemeriksaan BTA.

scholarly journals HASIL DIAGNOSTIK MYCOBACTERIUM TUBERCULOSIS DARI SPUTUM PENDERITA BATUK ≥ 2 MINGGUN DENGAN PEWARNAAN ZIEHL NEELSEN DI PUSKESMAS PANIKI BAWAH, TIKALA BARU DAN WONASA MANADO

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Maykel Sondak ◽  
John Porotu’o ◽  
Heriyannis Homenta
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Positive Result ◽  
Lung Disease ◽  
Health Center ◽  
Sampling Method ◽  
Acid Fast Bacillus ◽  
Age Group ◽  
Main Symptom ◽  
Descriptive Research

Abstrak: Batuk merupakan salah satu gejala penyakit paru yang paling penting, tetapi kadang tidak diperdulikan. Batuk ≥ 2 minggu perlu dicurigai karena merupakan gejala utama dari tuberkulosis paru terutama yang disertai dahak. Untuk mengetahui hasil diagnostik Mycrobacterium tuberkulosis pada sputum penderita batuk ≥ 2 minggu dengan pewarnaan Ziehl-Neelsen di Puskesmas Paniki Bawah, Tikala Baru, dan Wonasa Manado. Desain penelitian yang digunakan adalah penelitian deskriptif dengan pengambilan sampel menggunakan cara total sampling pada kurun waktu september 2015 – Desember 2015. Pada pemeriksaan BTA pasien batuk ≥ 2 minggu di Puskesmas Paniki ditemukan 2 pasien (11,76%) positif dan 15 pasien (88,24%) negatif, di Puskesmas Tikala Baru ditemukan 1 pasien (7,69%) positif dan 12 pasien (92,31%) negatif, dan di Puskesmas Wonasa tidak ditemukan BTA positif, presentase berdasarkan jenis kelamin dengan batuk lebih dari dua minggu terbanyak pada jenis kelamin laki-laki 21 (52,5%) daripada perempuan 19 (47,5%), sedangkan presentase usia dengan batuk lebih dari dua minggu terbanyak terjadi pada usia 55-79 tahun 37,5%. Kesimpulan: Batuh lebih dari dua minggu merupakan gejala dari Tubekulosis paru dan paling sering diderita laki-laki dibandingkan perempuan pada kelompok usia 55-79 tahun.Kata kunci: batuk lebih dari dua minggu, tuberculosis, BTAAbstract: Cough is a symptom of lung disease which is very important, but sometimes ignored. Cough more than 2 weeks need to be taken seriously because it is the main symptom of pulmonary tuberculosis, especially when the cough is accompanied by phlegm. To determine the diagnostic results of mycrobacterium tuberculosis in the sputum of patients with cough ≥ 2 weeks with Ziehl-Neelsen colouration in Puskesmas Paniki bawah, Tikala Baru, and Wonasa Manado. The study design used descriptive research by sampling method using total sampling during the period September 2015 - December 2015. patients with cough more than two weeks by checking the acid fast bacillus method in Paniki health center, we found 2 patients (11,76%) with positive result and 15 patients (88,24%) negative, whereas in Tikala found 1 patients (7,69%) positive and 12 patients negative, in addition in Wonasa we don’t find any positive patients. Percentage by sex with a cough for more than two weeks we found mostly male with 21 patients (52.5%) while women only 19 patients (47.5%). From the percentage of age with cough for more than two weeks mostly occurred in age 55-79 years old with percentage of 37.5%.Conclusion: Cough more than two weeks is a symptom of lung Tubekulosis and most often affects males than females in the age group 55-79 years. Keywords: cough more than two weeks, tuberculosis, BTA

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