scholarly journals Perbandingan Aktivitas Antioksidan Dari Tongkol Jagung (Zea mays L.) Segar dan Kering Dengan Metode Refluks

Jurnal MIPA ◽  
2014 ◽  
Vol 3 (2) ◽  
pp. 92
Author(s):  
Ryan Vanly Saryana ◽  
Edi Suryanto ◽  
Meiske S. Sangi
Keyword(s):  
Condensed Tannins ◽  
Zea Mays L ◽  
Water Extract ◽  
Free Radical Scavengers ◽  
Total Phenolic ◽  
Corn Cob ◽  
Corn Cobs ◽  
Water Solvent ◽  
Solvent Analysis

Tujuan pada penelitian ini adalah menentukan potensi antioksidan ekstrak air dari tongkol jagung. Tongkol jagung yang sudah dikering anginkan digiling sedangkan yang segar hanya dicincincang hingga halus kemudian diekstraksi menggunakan metode refluks selama 0, 1, 2, 3 dan 4 jam menggunakan pelarut akuades. Analisis kandungan total fenolik, flavonoid dan tanin terkondensasi menggunakan spektrofotometer UV-Vis. Untuk penentuan penangkal radikal bebas menggunakan metode 1.1-diphenyl-2.-picrylhydrazyl (DPPH). Hasil dari penelitian ini menunjukkan bahwa tongkol jagung kering memiliki kandungan total senyawa fenolik, flavonoid dan DPPH  terbaik dengan pemanasan selama 4 jam. Akan tetapi total tanin terkondensasi terbaik terdapat pada tongkol jagung segar dengan pemanasan selama 4 jam.The purpose of this study was to determine the antioxidant potential of water extract of corn cobs. Corn cobs wind dried milled while fresh only chopped until smooth then extracted using the reflux method for 0, 1, 2, 3 and 4 hours using distilled water solvent. Analysis of total phenolic content, flavonoids and condensed tannins using UV-Vis spectrophotometer. For the determination of free-radical scavengers using the method of 1.1-diphenyl-2-picrylhydrazyl (DPPH). The results of this study indicate that the dried corn cobs have the best content of total phenolic compounds, flavonoids and DPPH by heating for 4 hours. But, the total condensed tannins are best fresh on corn cob with heating for 4 hours.

scholarly journals Comparison of total phenolic and flavonoids contents in Malaysian propolis extract with two different extraction solvents

2019 ◽  
Vol 6 (2) ◽  
pp. 1-11
Author(s):  
Siti Umairah Mokhtar
Keyword(s):  
Colorimetric Method ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Extraction Process ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Propolis Extract ◽  
Extraction Solvent ◽  
Percentage Yield

This study is aimed to prepare propolis extracts by using two different solvents which are ethanol and water via maceration method and to evaluate the total phenolic and flavonoid components of the extract. Optimization of the extraction process was done by using different solvents and sample-to-solvent ratio. The ethanolic and water extracts were analysed for the determination of Total Phenolic Content (TPC) and Total Flavonoid Content (TFC) by using Folin-Ciocalteu colorimetric method and Aluminimum chloride method, respectively. Based on sample-to-solvent ratio, it showed that 1:10 gave the highest percentage yield compared to other ratio for both solvents. Ethanolic extract resulted contains high phenolic and flavonoids content compared to water extract. The result showed that sample-to-solvent ratio 1:5 gave highest TPC and TFC in ethanolic extract which are 29.09 and 7.75 mg GAE/g, respectively. In conclusion, this study revealed that ethanol is the best extraction solvent to be used for propolis extraction compared to water. It is also demonstrated that higher volume of solvent does not leads to high values of TPC and TFC. The optimum extraction parameters and conditions are necessary to be obtained in order to extract high TPC and TFC.

scholarly journals Evaluation of physicochemical properties of anthocyanin extracts and powders from purple sweet potato (Ipomoea batatas L.)

Food Research ◽  
2020 ◽  
Vol 4 (6) ◽  
pp. 2020-2029
Author(s):  
T. Ekaputra ◽  
R. Pramitasari
Keyword(s):  
Citric Acid ◽  
Sweet Potato ◽  
Water Extract ◽  
Egg White ◽  
Acid Water ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Water Solvent ◽  
Purple Sweet Potato

Purple sweet potato (Ipomoea batatas L.) is one of the food commodities that contain anthocyanin. This research aimed to evaluate the physicochemical properties of anthocyanin extracts and powders from purple sweet potato. Purple sweet potato anthocyanin was extracted using a water solvent with the addition of citric acid in different concentrations (0, 2, 4, and 6 g/100 mL). Ethanol solvent was used as a control. Foam mat drying was performed using variations of egg white composition (10 and 20 mg/100 mL). The result showed that extraction using a water solvent with 2 g/100 mL of citric acid was the most effective condition to obtain the highest total anthocyanin content (p < 0.05). However, its total phenolic, flavonoid, and antioxidant activity were lower compared to others. Total anthocyanin content of foam mat dried purple sweet potato powder made from the citric acid-water extract was significantly higher than the powder made from the citric acid-ethanol extract (p < 0.05). There was no effect in the total phenolic, flavonoid, viscosity, and yellowish-red color of the powder obtained from citric acid-water extract as the increase of egg white concentration. The use of 20 g/100 mL egg white in both powders produced from citric acid-water and ethanol extracts resulted in higher total anthocyanins and solubilities than powders using 10 g/100 mL egg white. The color of the powder obtained from citric acid-water extract had a lower brightness level and higher yellowish-red color intensity compared to the powder made from citric acidethanol extract.

Extract of Trema Orientalis (L.) Stem Bark: A Potential Source of Environmentally Friendly Tanning Agent for Leather Industry

2022 ◽  
Vol 117 (1) ◽  
Author(s):  
Murshid Jaman Chowdury ◽  
Md. Abdur Razzaq ◽  
Md. Imran Biswas ◽  
Ariful Hai Quadery ◽  
Md. Tushar Uddin
Keyword(s):  
Water Extract ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Elevated Concentration ◽  
Tannin Content ◽  
Chemical Additives ◽  
Water Solvent ◽  
Trema Orientalis ◽  
Vegetable Tanning

Researchers around the world are in continuous endeavor to develop environment friendly tanning agent due to adverse effect of conventionally used chromium during leather tanning. Recent trend of tanning is thus heading to chrome-free and greener chemical processing options. Vegetable tanning is an exoteric leather processing technique because of their lower pollution load on the environment. Considering the inadequacy and high costing for the commercialized tannins, development of alternative tannins from locally available plants and their characterizations are important. In this research, the stem barks of Trema Orientalis (L.) were extracted at different temperatures employing water solvent with or without additives (sodium hydroxide or sodium sulphite) to attain phenolic-rich extractives. To ascertain appropriateness as a vegetable tanning agent, the obtained extracts were thereafter characterized in respect to yield, total phenolic content, tannin content and molecular structure. The extraction yield for all extracts improves and the quality remains nearly unchanged with temperature rising. The elevated concentration of chemical additives enhances the extraction yield but lessens the quality of extracts. In terms of extraction yield and the quality of extracts the best condition for extraction was discerned at 80°C with water solvent. This water extract has a decent extent of phenolic and tannin content of 266.13 mg Gallic acid equivalent/ gm of dry extract and 30.12 % respectively. The final extract exhibits excellent leather retanning tendency comparable to the commercial quebracho tannins.

scholarly journals Sunscreen Cream Formulation from Corn Cob Extract (Zea maysM/em> L.) and Robusta Coffee Bean Extract (Coffea canephora Pierre ex A. Froehner)

2020 ◽  
Vol 8 (6) ◽  
Author(s):  
Yetri Elisya ◽  
Harpolia Cartika ◽  
Adin Hakim
Keyword(s):  
Zea Mays L ◽  
Total Phenolics ◽  
Coffea Canephora ◽  
Total Phenol ◽  
Coffee Bean ◽  
Corn Cob ◽  
Robusta Coffee ◽  
Ic50 Value

The results of previous studies on each concentration of 4000 ppm SPF value of sunscreen cream corncob extract (Zea mays L.) of 5.40. and SPF value of coffee bean extract cream (Coffea canephora Pierre ex A. Froehner) of 8.54. Both of these plant research results have SPF values that are still below 8 -15, so that further research is carried out using a mixture of the two extracts on a sun cream basis based on M / A basis. Methods: Making extracts of corncob mixture and Robusta coffee using a dose of  10%, and 15% and using a type A/M cream base. Cream testing includes physical and stability tests. Determination of the effectiveness of sunscreen cream preparations is done by testing the SPF value, antioxidant content and total phenolics in vitro using a UV / Vis spectrophotometer. Results: Cream stability test meets cream requirements. Formula 1 has an IC50 value of 198.61 ppm, a total phenol of 46 mGAE / g, at an SPF value of 35. Formula 2 has an IC50 value of 101.57 ppm, a total phenol of 59.9 mGAE / g, an SPF value of 38. Formula 3 has an IC50 value of 150.26 ppm, a total phenol of 51.45 mGAE / g and SPF 37. Conclusion: Sunscreen cream corn cobs extract and Robusta coffee has good stability, strong antioxidants, the highest phenol in formula 2, and are categorized as ultra sunblock  

scholarly journals Total Phenol and Antioxidant Activity of Ethanol Extract and Water Extract from Claw Uncariaa gambir Roxb

2021 ◽  
Vol 9 (3) ◽  
pp. 131
Author(s):  
Lucky Hartanti ◽  
Asri Mulya Ashari ◽  
Warsidah Warsidah
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Water Extract ◽  
Ethanol Extract ◽  
Total Phenol ◽  
Total Phenolic ◽  
Market Demand ◽  
University Campus ◽  
Industrial Plants

Uncaria gambier Roxb is a plant from the Rubiaceae family, belongs to the Bajakah group and has been used empirically in the treatment of various types of diseases in the community. Besides being one of the industrial plants and export commodities produced in Sumatra and Kalimantan, gambier leaves is also used frequently in Indonesia, both for the pharmaceutical and cosmetic industries. As a result, market demand both nationally and internationally have increased. The aim of this research are determination of antioxidant activity of ethanol extract and aqueous extract of gambir claw using 1,1-diphenil-2-picrylhydrazyl (DPPH) and determination of total phenol using Calcetau folin reagent. Gambier plant samples were taken in the forest area around the Tanjungpura University campus, the claws were separated and dried at room temperature, then powdered and extracted by maceration using 70% ethanol pa and aquadest, then the extract was concentrated until it was ready to be tested for antioxidant activity and determination of total phenol. The total phenolic ethanol extract and aqueous extract of gambir claw were 224.66 mg GAE/g extract (GEA : Gallic Acid Eqivalent) and 299.08 mg GAE/g extract, respectively, while the antioxidant potential (IC50) was 39.566 μg/mL and 65.140 μg/mL, and the IC50 for comparison of Vitamin C was 7.02 μg/mL.

scholarly journals ARTEMISININ CONTENT ON ARTEMISIA ANNUA L. TREATED BY GLORIOSA SUPERBA SEEDS’ WATER EXTRACT

2018 ◽  
Vol 11 (8) ◽  
pp. 147 ◽  
Author(s):  
Sri Indah R ◽  
Ari Susilowati ◽  
Yuli Widyastuti ◽  
Ahmad Yunus
Keyword(s):  
Artemisia Annua ◽  
Treatment Plant ◽  
Water Extract ◽  
Gloriosa Superba ◽  
Soaking Time ◽  
Artemisia Annua L ◽  
Water Solvent ◽  
Artemisinin Content ◽  
Plant Treatment

Objective: The aim of this study was to determine the artemisinin content on Artemisia annua L. treated by water extract of Gloriosa superba seeds.Methods: G. superba seeds obtained naturally on Krakal Beach, Gunung Kidul, and extraction used a maceration method by water solvent (1:1). A. annua L. sprouts were obtained from B2P2TOOT Tawangmangu. Treatment variables done on sprouts using various water extract concentration of G. superba seeds and soaking time on A. annua L. sprouts. Determination of artemisinin content in leaf extract of A. annua L. was done using KLT-densitometric method with n-hexane:ethyl acetate (4:1) as mobile phase.Result: The result showed that artemisinin content in plant treatment of G. superba seed water extract was higher (9.78 μg/μl [±3.21]–16.60 μg/μl [±1.39]) compared to control plants (6.39 μg/μl [±1.40]). The concentration water extract of G. superba seed affected the level of artemisinin in the treatment plant. On the other hand, the soaking of A. annua L. sprouts using the water extract of G. superba seed did not affect the level of artemisinin content.Conclusion: Artemisinin content in treatment plant by G. superba seed water extract treatment was higher compared to control plants.

The Comparative Study of Equisetum pratense, E. sylvaticum, E. telmateia: Accumulation of Silicon, Antioxidant and Antimicrobial Screening

2019 ◽  
Vol 70 (7) ◽  
pp. 2519-2523
Author(s):  
Denisa Batir Marin ◽  
Oana Cioanca ◽  
Mihai Apostu ◽  
Cristina Gabriela Tuchilus ◽  
Cornelia Mircea ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Methanol Extract ◽  
Previous Treatment ◽  
Total Phenolic ◽  
Quantitative Chemical Analysis ◽  
Qualitative And Quantitative ◽  
Antioxidant And Antimicrobial Activity ◽  
The Comparative Study

The objective of the current study is represented by the determination of silica and a phytochemical screening of phenolic derivates of some Equisetum species. The antioxidant and antimicrobial activity for Equisetum pratense Ehrh.,, Equisetum sylvaticum L. and Equisetum telmateia Ehrh. (sin. Equisetum maximum Lam.) were also investigated. The concentration of silicon (Si) in plants was determined by the spectrophotometric method using previous treatment with NaOH 50% both for the stem and the nodal branches [1]. Results obtained varied from 95.12 to 162.10 SiO2 mg/g dry plant which represents 4.44% to 7.58% Si/100g dry sample. Two types of total extracts were obtained using different solvents and were subjected to qualitative and quantitative chemical analysis considering total phenolic content [2]. The highest concentration of investigated compounds was found in the methanolic extract, E. sylvaticum, 196.5mg/g dry sample. Antioxidant activity was monitored spectrophotometrically and expressed in terms of IC50 (�g/mL) [3]. Values gathered ranged from 261.7 to 429.5 �g/mL. The highest capacity to neutralized DPPH radicals was found in E. sylvaticum. In vitro antimicrobial activity was determined using difusimetric method [4]. Testing was performed on four microorganisms: three strains of bacteria and one species of fungi. Different effects were noticed against the bacteria, furthermore the methanol extract appeared to be most efficient. All extracts showed significand antimicrobial activity against Staphylococcus aureus (ATCC 25923) and Candida albicans (ATCC 90028) and weak to no activity against Pseudomonas aeruginosa (ATCC 27853) and Escherichia coli (ATCC 25922).

Biochemical study of attached macroalgae from the Madeira Archipelago and beach-cast macroalgae from the Canary Islands: multivariate analysis to determine bioresource potential

2020 ◽  
Vol 63 (3) ◽  
pp. 283-298
Author(s):  
Nuno Nunes ◽  
Sofia Valente ◽  
Sónia Ferraz ◽  
Maria Carmo Barreto ◽  
Miguel A.A. Pinheiro de Carvalho
Keyword(s):  
Biochemical Study ◽  
Algal Species ◽  
Biochemical Properties ◽  
Total Phenolic ◽  
Total Carotenoids ◽  
The North ◽  
Madeira Archipelago ◽  
Total Carbohydrates ◽  
Beach Cast

AbstractFifteen attached macroalgae from the Madeira Archipelago, comprising three green, three red and nine brown algal species, as well as two beach-cast macroalgal samples, collected along the north shore of Gran Canaria, were assessed for their biochemical properties. The analysis included the determination of total minerals, total carbohydrates, protein, lipids, chlorophyll a, total carotenoids, total phenolic content, fucoxanthin and phycobilins (allophycocyanin, phycocyanin and phycoerythrin). The results showed a high variability of biochemical composition, allowing for the targetting of specific bioresources for particular purposes, including functional foods. This work provides the foundation for a biorefinery strategy implementation plan, for which specific macroalgae may be targeted for valuable and beneficial compounds.

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