scholarly journals Panjang Dan Volume Akar Tanaman Padi Lokal Sulawesi Utara Saat Kekeringan Yang Diinduksi Dengan Polietilen Glikol 8000

Jurnal MIPA ◽  
2018 ◽  
Vol 7 (2) ◽  
pp. 12
Author(s):  
Crestie T. Mangansige ◽  
Nio Song Ai ◽  
Parluhutan Siahaan

Kekeringan berdampak buruk terhadap produktivitas dan produksi pangan di dunia. Penelitian ini bertujuan untuk mengkaji ada tidaknya perbedaan respons morfologis berdasarkan panjang dan volume akar pada tanaman padi lokal Sulawesi Utara (varietas Superwin, Ombong, Temo, dan Burungan) yang mengalami kekeringan dengan induksi PEG 8000. Tanaman padi dengan empat daun yang telah berkembang penuh pada fase vegetatif diberi perlakuan kekeringan yang diinduksi dengan PEG 8000 (potensial air/PA medium 0; -0,25; -0,5 MPa) selama 6 jam. Hasil penelitian menunjukkan bahwa faktor varietas, faktor konsentrasi PEG 8000, faktor waktu perlakuan, interaksi antara faktor varietas dan konsentrasi PEG, interaksi antar faktor varietas dan waktu perlakuan, interaksi antara faktor konsentrasi dan waktu perlakuan, interaksi antara faktor varietas, konsentrasi PEG dan waktu perlakuan tidak menyebabkan perbedaan panjang dan volume akar. Dalam penelitian ini  panjang dan volume akar tidak dapat dijadikan sebagai indikator cekaman kekeringan pada keempat varietas padi lokal Sulawesi Utara.Drought decreases the productivity and production of food in the world. This study aimed to evaluate the differences of morphological responses based on root length and volume in North Sulawesi local rice (cv. Superwin, Ombong, Temo, dan Burungan) under PEG-8000-induced drought. The 4-fully-expanded-leaf plants at the vegetative phase were treated by growing the plants in the PEG 8000 solution with water potential/PA 0; -0.25; -0.5 MPa or 6 hours. The results showed that factors of variety, PEG 8000 concentration, treatment period, interaction between variety and PEG 8000 concentration, interaction between variety and treatment period, interaction between PEG 8000 concentration and treatment period, interaction among variety, PEG 8000 concentration and treatment period did not result in any differences in root length and volume. Root length and volume were not able to be used as drought indicators of in these four North Sulawesi local rice

Author(s):  
Song Ai Nio ◽  
MONALISA Pirade ◽  
DANIEL PETER MANTILEN Ludong

Abstract. Nio SA, Pirade M, Ludong DPM. 2019. Leaf chlorophyll content in North Sulawesi (Indonesia) local rice cultivars subjected to polyethylene glycol (PEG) 8000-induced water deficit at the vegetative phase. Biodiversitas 20: 2462-2467. Climate change can result in drought stress in the environment and this condition reduces rice production. Polyethylene glycol (PEG) is used to induce water deficit, because it is able to decrease water potential in the solution. The present study aimed to evaluate the physiological response of North Sulawesi local rice (cvs. Superwin, Ombong, Burungan, and Temo) to PEG 8000-induced water deficit based on the leaf chlorophylls (a, b and total chl.) content at the vegetative phase. The results of this study showed that PEG 8000-induced water deficit with media water potential (WP) -0.25 and -0.5 MPa decreased the content of leaf total chlorophyll and leaf chlorophyll a. The longer treatment period could reduce the contents of leaf total chlorophyll, chlorophyll a and chlorophyll b. The content of leaf chlorophyll total and chlorophyll a were potential physiological indicators for North Sulawesi local rice response to PEG 8000-induced water deficit.


2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Monalisa Pirade ◽  
Song Ai Nio ◽  
Parluhutan Siahaan

Abstrak Perubahan iklim menjadi salah satu penyebab terjadinya kekeringan dan kondisi ini dapat menurunkan produksi padi. Polietilen glikol (PEG) mampu menurunkan potensial air dalam larutan, sehingga dapat digunakan untuk menginduksi kekeringan. Penelitian ini bertujuan untuk mengkaji ada tidaknya perbedaan respons fisiologis berdasarkan kandungan air daun pada tanaman padi lokal Sulawesi Utara yang mengalami kekeringan dengan induksi PEG 8000. Hasil penelitian ini menunjukkan bahwa faktor varietas (Superwin, Burungan, Ombong, dan Temo), faktor konsentrasi PEG 8000 (potensial air medium 0, –0,25 dan –0,5 MPa), faktor waktu perlakuan (0, 6 dan 12 jam), interaksi antara faktor varietas dan konsentrasi PEG, interaksi antara faktor varietas dan waktu perlakuan, interaksi antara faktor konsentrasi dan waktu perlakuan, interaksi antara faktor varietas, konsentrasi PEG, dan waktu perlakuan dalam cekaman kekeringan tidak menyebabkan perbedaan kandungan air daun yang nyata. Dalam penelitian ini  kandungan air daun tidak dapat dijadikan sebagai indikator respons fisiologis pada keempat varietas padi lokal Sulawesi Utara terhadap cekaman kekeringan. Kata kunci: cekaman kekeringan, PEG, kandungan air daun, padi lokal Sulawesi Utara  AbstractClimate change can result in drought and this condition can reduce rice production. Polyethylene glycol (PEG) decreases the water potential in the nutrient solution, therefore, it can be used to induce drought. The purpose of this research was to evaluate physiological response of North Sulawesi local rice under PEG 8000-induced drought based on the leaf water content.  The results of this study  indicated that varieties factor (Superwin, Ombong, Burungan and Temo), the factor of PEG 8000 concentration (medium water potential -0.25 and -0.5 MPa),  the treatment period factor (0, 6 and 12 hours), the interaction between varieties and PEG 8000 concentration, interaction between varieties and treatment period, the interaction between PEG 8000 concentration and treatment period, interaction among varieties, PEG 8000 concentration and treatment period under drought did not result in significant differences in leaf water content. This study showed that leaf water content was not able to be the physiological indicator in these four North Sulawesi local rice varieties under drought.Keywords: Drought, PEG, leaf water content, North Sulawesi local rice


2018 ◽  
Vol 18 (2) ◽  
pp. 63
Author(s):  
Risa Junita Mereh ◽  
Nio Song Ai ◽  
Parluhutan Siahaan

KANDUNGAN AIR PADA SEGMEN DAUN TANAMAN PADI (Oryza sativa L.) LOKAL SULAWESI UTARA SAAT KEKERINGAN YANG DIINDUKSI DENGAN POLIETILEN GLIKOL 8000 ABSTRAKPenelitian ini telah dilakukan untuk mengkaji kandungan air sebagai respon fisiologi pada segmen daun tanaman padi lokal Sulut (varietas Temo, Ombong, Burungan, dan Superwin) terhadap kekeringan yang diinduksi dengan PEG 8000 secara in vitro. Segmen daun padi dipotong-potong 1 cm x 1 cm dan diberi tiga macam perlakuan PEG 8000 (dengan potensial air/PA medium 0; -0,25; dan -0,5 MPa), dan empat waktu pengambilan sampel (0, 4, 8 dan 12 jam) dalam tiga kali ulangan. Hasil penelitian menunjukkan bahwa bahwa faktor varietas, faktor waktu perlakuan,  faktor konsentrasi PEG 8000, dan interaksi antara ketiga faktor tersebut tidak menyebabkan perbedaan kandungan air segmen daun yang nyata.Kata kunci: Cekaman kekeringan, PEG 8000, Potensial air, Segmen daun LEAF WATER CONTENT IN LEAF SEGMENTS OF NORTH SULAWESI LOCAL RICE (Oryza sativa L.)  UNDER POLYETHYLENE-GLYCOL- 8000-INDUCED DROUGHTED DROUGHTABSTRACTA study was conducted to evaluate leaf water content as one of physiological responses in leaf segments of North Sulawesi local rice (cv. Temo, Ombong, Burungan, and Superwin) under PEG 8000- induced-drought. The rice leaves were cut into 1 cm x 1 cm segments and treated withPEG 8000 solutions  (medium water potential or PA of 0; -0.25; and -0.5 MPa),four sampling times (0, 4, 8 and 12 hours) and 3 replicates. The results indicated that factors of varieties, treatment period, concentration of PEG 8000, and interaction among these factors did not result in any differences in water content of rice leaf segments.Keywords: Drought stress, PEG 8000, Water potential, Leaf segment


Irriga ◽  
2018 ◽  
Vol 1 (01) ◽  
pp. 246
Author(s):  
Lígia Borges Marinho ◽  
José Antonio Frizzone ◽  
João Batista Tolentino Júnior ◽  
Janaina Paulino ◽  
Danilton Luiz Flumigan ◽  
...  

DINÂMICA DA ÁGUA NO SISTEMA SOLO-PLANTA NO CULTIVO DA PIMENTA TABASCO SOB DÉFICIT HÍDRICO1  LÍGIA BORGES MARINHO2; JOSÉ ANTONIO FRIZZONE3; JOÃO BATISTA TOLENTINO JÚNIOR4; JANAÍNA PAULINO5; DANILTON LUIZ FLUMIGNAN6 E DIEGO BORTOLOTI GÓES3    (1) Artigo extraído da tese do primeiro autor (2) Departamento Tecnologia e Ciências Sociais, Universidade do Estado da Bahia, av. Edgard Chastinet, São Geraldo, CEP 48905-680, Juazeiro, BA. Fone (74) 3611-7363. E-mail: [email protected](3) Departamento de Engenharia de Biossistemas/Escola Superior de Agricultura “Luiz de Queiroz” USP, Av. Pádua Dias, 11, CEP 13.418-900, Piracicaba/SP, E-mail(s): [email protected], [email protected]; (4) Campus Curitibanos, Universidade Federal de Santa Catarina, Curitibanos, SC. [email protected] (5) Universidade Federal de Mato Grosso UFMT, campus Sinop, Avenida Alexandre Ferronato Nº 1.200. Bairro: Setor Industrial. CEP: 78.550-000,  Sinop-MT, Email: [email protected] (6) Empresa Brasileira de Pesquisa Agropecuária, Agropecuária Oeste. Rodovia BR 163, km 253, Zona Rural, 79804970 - Dourados, MS,  Email: [email protected]  1 RESUMO  O objetivo da pesquisa foi acompanhar a variação da condição hídrica do solo e da planta de pimenta ‘Tabasco’ em função dos manejos de déficits hídricos impostos e determinar seu coeficiente de estresse hídrico. O experimento foi conduzido em ambiente protegido, no Departamento de Engenharia de Biossistemas da ESALQ - USP, Piracicaba-SP, de setembro de 2009 a julho de 2010. O delineamento experimental foi blocos casualizados, com quatro repetições, utilizando-se lâminas de irrigação a 100, 80, 60 e 40% da evapotranspiração da cultura diferenciadas a partir da fase vegetativa e da fase reprodutiva. O potencial da água na folha e no solo foi aferido com a câmara de pressão e tensiômetros, respectivamente. Houve variação do potencial mátrico, da extração de água no solo e do potencial de água na folha em função das lâminas e das épocas de diferenciação. Menores potenciais mátricos foram verificados quando o déficit de irrigação foi inicializado na fase vegetativa da pimenta. Os valores de coeficiente de estresse hídrico e o potencial de água na folha, ao alvorecer, indicaram que as pimenteiras estavam sob estresse moderado e severo, sendo a época reprodutiva da pimenta Tabasco a mais sensível à restrição hídrica.Palavras-chave: Capsicum frutencens L, tensiômetro, potencial da água no solo.                                                        MARINHO, L. B.; FRIZZONE, J. A.; TOLENTINO JÚNIOR, J. B.; PAULINO, J.; FLUMIGNAN, D. L.; GÓES, D. B.WATER DYNAMICS IN SOIL-PLANT SYSTEM IN THE CULTIVATION OF PEPPER TABASCO UNDER WATER DEFICIT  2 ABSTRACT The objective of the research was to determine the change in soil water condition and in Tabasco pepper plant according to the managements of water deficits. The experiment was conducted in a greenhouse at the Department of Biosystems Engineering of ESALQ - USP, Piracicaba-SP, from September 2009 to July 2010. The experimental design was randomized blocks with four replications, using irrigation depths to 100, 80, 60 and 40% of crop evapotranspiration in the vegetative phase and reproductive phase. The soil matric potential was measured by tensiometers installed at 0-20 and 20-40 cm depth. The most negative values of matric potential occurred in treatments submitted to the greater water deficit treatments that had higher water restriction imposed by the vegetative phase. For these, greater increase in water extraction in the deepest layer (40 cm) were also found.There were differences in matric potential of the soil, in ground water extraction and in leaf water potential in relation to the water depths and differentiation phases. The deficit irrigation that started in the vegetative phase led to greater reduction in soil matric potential due to the accumulated water deficit. The pepper plants have moderate to severe sensitivity to water deficit in the soil, with a higher sensitivity of the plants when water restriction is imposed during reproductive stages than when it is imposed during growing stages. Keywords : Capsicum frutencens, tensiometer; soil water potential


2007 ◽  
Vol 11 (1) ◽  
pp. 46-52 ◽  
Author(s):  
Eugênio F. Coelho ◽  
Delfran B. dos Santos ◽  
Carlos A. V. de Azevedo

This research had as its objective the investigation of an alternative strategy for soil sensor placement to be used in citrus orchards irrigated by micro sprinkler. An experiment was carried out in a Tahiti lemon orchard under three irrigation intervals of 1, 2 and 3 days. Soil water potential, soil water content distribution and root water extraction were monitored by a time-domain-reflectometry (TDR) in several positions in soil profiles radial to the trees. Root length and root length density were determined from digital root images at the same positions in the soil profiles where water content was monitored. Results showed the importance of considering root water extraction in the definition of soil water sensor placement. The profile regions for soil water sensor placement should correspond to the intersection of the region containing at least 80% of total root length and the region of at least 80% of total water extraction. In case of tensiometers, the region of soil water potential above -80 kPa should be included in the intersection.


2017 ◽  
Vol 1 (2) ◽  
pp. 73
Author(s):  
Ronan Carlos Colombo ◽  
Vanessa Favetta ◽  
Ricardo Tadeu de Faria

The vegetative propagation of orchids allows for the collection of plants genetically identical to the mother plant and reduces their juvenile period. Among the most commercialized orchids in the world, the Dendrobium nobile and its hybrids stands out among. Therefore, the objective of this work was to study the propagation of this species through pseudobulb cuttings. Cuttings with two or three buds (knots) were collected from adult plants and stacked in sand, horizontally and vertically, constituting a 2 x 2 factorial scheme, number of buds x cuttings position; with four replications of 15 cuttings. Six months later, the percentage of sprouted cuttings, number and length of shoots per cutting and root length were assessed, but no interaction or diff erences between treatments were found. The use of cuttings with two buds is recommended to optimize the propagation material and further collection of greater number of plants.


1988 ◽  
Vol 28 (2) ◽  
pp. 249 ◽  
Author(s):  
S Fukai ◽  
P Inthapan

Several physiological responses were compared, under irrigated and water-stressed conditions, in an attempt to explain the reasons for the greater reduction in dry matter production of rice compared with maize and sorghum in a water-limiting environment. Leaf water potential and leaf rolling were determined weekly, soil water profiles and root length density twice, and leaf osmotic potential once during a long dry period. Root length density of rice was at least as high as that of maize and sorghum in the top 0.6 m layer of soil in both the wet and dry trials. There was no difference in water extraction among the 3 species from this layer, while rice extracted less water than did the other species from below 0.6 m. High variability among replicates precluded any conclusion being drawn regarding root length in the deeper layer. Leaf water potential, measured in the early afternoon, was consistently lower in rice than in maize and sorghum, even when soil water content was high, indicating high internal resistance to the flow of water in the rice plants. The low leaf water potential in rice was accompanied by low osmotic potential, and this assisted in maintenance of turgor and dry matter growth when soil water content was relatively high. As soil water content decreased, however, leaf water potential became very low (less than - 2.5 MPa) and, for rice, leaves rolled tightly.


2015 ◽  
Vol 4 (1) ◽  
pp. 42-57
Author(s):  
Ivonne Angelic Umboh ◽  
Vincent Didiek Wiet Aryanto

North Sulawesi's tourist destination image is imperative to be promoted to the rest of the world, it is one of the top ten tourist destination in Indonesia. The tourism objects in North Sulawesi are varying such as culinary, undersea coral diving in Bunaken and Lembe, festivals, rituals both Christian and Muslim, eco-tourism, mountain climbing, jungle explorer, variety of culture's ethnic groups, tropical panorama etc. North Sulawesi is also known to have abundant of spice (nutmeg) and one of the biggest sea coral treasures in the world. Promotion variables are composed of advertising, direct marketing, festivals, events and experiences, cooperation with other parties, promotion by word-of-mouth, and tourist destination websites. 310 foreign tourist visitors from various country of origin were drawn as sample in this study. This study reveals that foreign tourists image on promotion undertaken by the government as well as private tourism industries vary to each variable being used in this study.


2012 ◽  
Vol 92 (2) ◽  
pp. 311-319 ◽  
Author(s):  
Cheng-Wu Jin ◽  
Yan-Lin Sun ◽  
Dong-Ha Cho

Jin, C.-W., Sun, Y.-L. and Cho, D.-H. 2012. Changes in photosynthetic rate, water potential, and proline content in kenaf seedlings under salt stress. Can. J. Plant Sci. 92: 311–319. As irrigation water salinization has become a serious constraint for crop production, as well as soil salinity, the selection and use of salt-tolerant species is urgently required. In this study, we describe the salt responses of three kenaf (Hibiscus cannabinus L.) cultivars and select the cultivar with high salt tolerance. Responses to salt stress were investigated in terms of growth, water potentials, photosynthesis, and proline contents. Photosynthesis, evaluated by net CO2 assimilation rate, stomatal conductance, and intercellular CO2 concentration, did not show any significant effects among the cultivars. Leaf water potential decreased depending on salt concentration and salt treatment period. Proline accumulation was enhanced, particularly depending on salt concentration, but not salt treatment period. As salt concentration increased, seedling growth was inhibited to a certain extent, and the inhibition of growth depended mainly on salt concentration. At the early stage of treatment, Dowling was more tolerant to salt stress than Everglade-41 and Tainung-2. Although Dowling showed a lower decrease in fresh weight, Tainung-2 always maintained a relatively high vegetative yield, even under high salt stress. Tainung-2, having greater salt adaptation, was therefore considered an ideal cultivar for popularization and farm cultivation.


2020 ◽  
Vol 36 (6) ◽  
Author(s):  
Joelmir Vital da Silva ◽  
Wilson Itamar Maruyama ◽  
Carlos Eduardo Da Silva Oliveira ◽  
Fabio Steiner ◽  
Alan Mário Zuffo ◽  
...  

The rubber tree (Hevea brasiliensis Müell. Arg.) is a species of significant economic interest in the natural rubber industry in Brazil and the world. This species presents recalcitrance to rooting, and its cuttings are difficult to propagate. This study aimed to evaluate the effect of the pre-conditioning of rubber tree mini-cuttings with zinc on the improvement of the adventitious rooting of rootstocks. Mini-cuttings were standardized with 45 mm length and submitted to preconditioning by immersion of the mini-cutting base in solutions containing 0.00; 0.04; 0.08; 0.16; 0.32 and 0.64 mg L-1 of Zn, for 24 hours. The experimental design was randomized blocks with six treatments and four replicates of 10 mini-cuttings. The rubber tree mini-cuttings were placed in a fitotron-type growth chamber, at 25 °C, with 12-hour photoperiod, 5,000 K intensity, and 95% of relative air humidity, for 60 days. The survival rate, number of buds, percentage of mini-cuttings that had leaf abscission, the percentage of mini-cuttings with callogenesis in the root meristem, the percentage of rooted mini-cuttings, the number of primary roots and root length were evaluated. The highest values of survival rate, the number of buds, the number of primary roots, the percentage of mini-cuttings with callogenesis in the root meristem, the percentage of rooted mini-cuttings and root length were observed with 0.16 to 0.26 Mg L-1 of Zn. The use of zinc in the mini-cuttings of rubber tree reduces linearly the percentage of mini-cuttings that had leaf abscission and the formation of callogenesis in the root meristem.   The rubber tree (Hevea brasiliensis Müell. Arg.) is a species of a large economic interest in the natural rubber industry in Brazil and the world. This species presents recalcitrance to rooting, and its cuttings are difficult to propagate. This study aimed to evaluate the effect of the pre-conditioning of rubber tree mini-cuttings with zinc on the improvement of the adventitious rooting of rootstocks. Mini-cuttings were standardized with 45 mm length and submitted to preconditioning by immersion of the mini-cutting base in solutions containing 0; 0.04; 0.08; 0.16; 0.32 and 0.64 mg L-1 of Zn, for 24 hours. The experimental design was randomized blocks with six treatments and four replicates of 10 mini-cuttings. The rubber tree mini-cuttings were placed in a fitotron-type growth chamber at 25 °C, with 24-hour photoperiod, 5,000 K intensity and 95% relative humidity for 60 days. The survival rate, number of buds, percentage of mini-cuttings that had leaf abscission, the percentage of mini-cuttings with callus, the percentage of rooted mini-cuttings, the number of primary roots and root length were evaluated. The highest values of survival rate, the number of buds, the number of primary roots, the percentage of mini-cuttings with callus, the percentage of rooted mini-cuttings and root length were verified with 0.16 to 0.26 Mg L-1 of Zn. The use of zinc in the mini-cuttings of rubber tree reduces linearly the percentage of mini-cuttings that had leaf abscission and the formation of root calluses.


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