scholarly journals EFFECT OF ADDITION OF DIFFERENT LEVELS OF FORMIC ACID AND UREA ON CHEMICAL COMPOSITION AND FERMENTATION CHARACTERISTICS OF WILD REED PHRAGMITIS COMMUNIS SILAGE

2019 ◽  
Vol 50 (5) ◽  
Author(s):  
Saeed & et al.
Keyword(s):  
Chemical Composition ◽  
Formic Acid ◽  
Crude Protein ◽  
Volatile Fatty Acids ◽  
Common Reed ◽  
Ammonia Nitrogen ◽  
Ph Values ◽  
Reed Plants ◽  
Fermentation Parameters ◽  
Different Levels

This study was carried out in the laboratory to investigate the effect of addition of different levels of formic acid (FA) and urea on chemical composition and fermentation characteristics of common reed silages. About 400-500g of silage samples were prepared by treating chopped reed plants (2-3 cm) with solutions containing 10% of date juice, 0.5, 1 or 1.5% of FM and 0, 1 or 2% of urea. Silage samples were packed in double nylon bags and stored anaerobically for 60 days. Results showed that green color was roughly dominant in most samples of silage with vinegar odor. Samples were well aggregated with little mold was observed in few urea untreated silages. Results revealed also that increasing level of  FA from 0.5 to 1 and 1.5%  increased (P˂0.01) contents of dry matter (DM) by 0.79 and 1.15%, and crude protein (CP) by 1.42 and 2.11% respectively, and decreased (P˂0.01) ether extract (EE), by 0.29 and 0.63%. About effect of urea levels, most variables pointed out that there was a decrease may be occurred in fermentations due to a significant decrease in contents of DM (P˂0.01) and EE (P˂0.05). Content of crude fiber (CF) was decreased (P˂0.01) from 44.7 to 43 and 41.1% for 0, 1and 2% levels of urea respectively. Results showed that there was a decrease (P˂0.01) in pH values from 5.90 to 4.99 and 4.88, concentrations of ammonia nitrogen (NH3-N), from 1.19 to 0.75 and 0.66% of total nitrogen and total volatile fatty acids (TVFA) from 6.56 to 4.61 and 4.14 mmol/100 g DM of silage samples as a result of addition of FA at levels of 0.5, 1 and 1.5% respectively. However, increasing urea levels from 0 to 1 and 2% associated with an increase (P˂0.01) in fermentation parameters, 5.02, 5.06 and 5.70 for pH, 0.67, 0.98 and 0.95 for NH3-N and 3.70, 5.53 and 6.07 mmol/100 g DM for TVFA respectively    

Effects of different levels of rapeseed cake containing high glucosinolates in steer ration on rumen fermentation, nutrient digestibility and the rumen microbial community

2020 ◽  
pp. 1-9
Author(s):  
Jian Gao ◽  
Yanfeng Sun ◽  
Yu Bao ◽  
Ke Zhou ◽  
Dehuang Kong ◽  
...  
Keyword(s):  
Microbial Community ◽  
Crude Protein ◽  
Volatile Fatty Acids ◽  
Latin Square ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Nutrient Digestibility ◽  
Rapeseed Cake ◽  
Operational Taxonomic Units ◽  
Different Levels

Abstract This trial was conducted to study the effects of dietary rapeseed cake (RSC) containing high glucosinolates (GLS) on rumen fermentation, nutrient digestion and the rumen microbial community in steers. Eight growing steers and four rations containing RSC (GLS 226·1 μmol/g DM) at 0·00, 2·65, 5·35 and 8·00 % DM were assigned in a replicate 4 × 4 Latin square design. The results indicated that increasing RSC levels increased the ruminal concentration of thiocyanate (SCN) (P < 0·01), decreased the ruminal concentration of ammonia nitrogen (NH3-N) and the molar proportion of isovalerate (P < 0·05), did not affect the ruminal concentration of total volatile fatty acids (P > 0·05), decreased the crude protein (CP) digestibility (P < 0·05) and increased the ether extract (EE) digestibility (P < 0·01). Increasing RSC levels tended to decrease the abundances of ruminal Ruminobacter amylophilus (P = 0·055) and Ruminococcus albus (P = 0·086) but did not affect methanogens, protozoa, fungi and other bacteria (P > 0·05). Increasing RSC levels in the ration did not affect the ruminal bacterial diversity (P > 0·05), but it increased the operational taxonomic units and the bacterial richness (P < 0·05) and affected the relative abundances of some bacteria at the phylum level and genus level (P < 0·05). In conclusion, RSC decreased the ruminal concentration of NH3-N and the CP digestibility, increased the EE digestibility and partly affected the ruminal bacterial community. SCN, as the metabolite of GLS, could be a major factor affecting these indices.

The effect of lipid sources on intake, rumen fermentation parameters and microbial protein synthesis in Nellore steers supplemented with glycerol

2014 ◽  
Vol 54 (10) ◽  
pp. 1871 ◽  
Author(s):  
Arturo Samuel Gomez Insuasti ◽  
Yury Tatiana Granja Salcedo ◽  
Pablo de Souza Castagnino ◽  
Bruno Ramalho Vieira ◽  
Euclides Braga Malheiros ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Ether Extract ◽  
Microbial Protein ◽  
Molar Proportion ◽  
Fermentation Parameters

The effects of glycerol with fat sources as a feed alternative were investigated in the ration for Nellore steers (Bos indicus). Eight cannulated steers at 30 months of age with initial bodyweight of 554 ± 36.0 kg were used in a double 4 by 4 Latin square design with four consecutive 16-day periods. Treatments were three different diets with lipid sources (soybean, soybean oil and calcium salts of fatty acids) and one diet control without lipid sources. All diets formulated contained 10% crude glycerol and 5% ether extract with a forage:concentrate ratio of 35:65; corn silage was used as forage. The ruminal liquid was sampled for 24 h and ruminal fermentations were monitored by measuring pH, concentrations of ammonia nitrogenand volatile fatty acids in rumen fluid. Urine samples were obtained from the total collection for 24 h for estimation of rumen microbial protein supply using urinary purine derivatives. Our results showed that the use of lipid sources combined with glycerol did not induce significant changes in rumen pH, acetate molar proportion, ruminal microbial protein or dry matter intake. Although the acetate molar proportion was kept constant within normal parameters, the propionate molar proportion was increased by the diet containing lipid sources. Moreover, we found that there was a negative effect of lipid sources on crude protein and neutral detergent fibre corrected for ash and protein intake, and one positive effect on ether extract intake. We believe that association between fat and glycerol may affect rumen fermentation parameters through reducing fibre intake and increasing propionate production and ammonia nitrogen.

EFFECTS OF PRESERVING ALFALFA FORAGE AS FORMIC-ACID-TREATED SILAGE, WILTED SILAGE, AND AS HAY IN LARGE ROUND BALES ON CHEMICAL COMPOSITION, RECOVERY OF NUTRIENTS, DIGESTIBILITY AND HEIFER GROWTH

1983 ◽  
Vol 63 (4) ◽  
pp. 925-937 ◽  
Author(s):  
A. S. ATWAL
Keyword(s):  
Chemical Composition ◽  
Formic Acid ◽  
Crude Protein ◽  
Insoluble Fraction ◽  
Corn Silage ◽  
Growth Study ◽  
Total N ◽  
Gross Energy ◽  
Large Round ◽  
Study Performance

First-cut alfalfa was stored as formic-acid-treated silage (FS), wilted silage (WS) and hay (Hay 1) in large round bales (LRB). Hay 1 was exposed to frequent showers (15.1 mm) in the windrows and required a drying period of 13 days; dry matter (DM) yield was only 60% of the average for silages (3.9 tonnes/ha). Second-cut alfalfa hay (Hay 2) yielded 1.84 tonnes DM/ha. For winter feeding, recoveries from storage of FS, WS, Hay 1 and Hay 2, respectively, were 85.7, 87.2, 78.3 and 81.9% of energy; 81.1, 87.0, 75.0 and 72.6% of crude protein (CP). Adlibitum intakes of DM were 1.90, 1.93, 1.78 and 2.08% of body weight for the four treatments, respectively. Differences in chemical composition and digestibility of nutrients for FS, WS and Hay 2 were small and statistically not significant (P > 0.05). However, values of FS were highest for CP (20.6%) and gross energy (20.5 kJ/g DM); digestibilities of DM, energy and CP (i.e. 59.3, 60.6 and 68.7% respectively) when fed at maintenance level; digestibilities of DM, energy, CP, cellulose and hemicellulose (i.e. 64.1, 66.6, 61.9, 64.5 and 46.3%, respectively) when fed in combination with corn silage (1:1 DM basis) on an ad libitum basis. All these values were significantly (P < 0.05) higher than the corresponding values for Hay 1. Heat damage to CP of hay stored in LRB was high and 19.9% of total N of Hay 1 was in the acid detergent insoluble fraction. In a growth study, performance of yearling heifers did not differ significantly when rations made of corn silage and FS or WS (1:1 DM basis) were compared. Key words: Alfalfa silages, wilted, digestibility, formic acid, round bales, heifer growth

scholarly journals Alfalfa Protein Concentrate as a Less Ruminally Degradable Protein Source for High-Producing Dairy Cows

1997 ◽  
Vol 2 ◽  
pp. 31
Author(s):  
C.D. Lu ◽  
NA. Jorgensen
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Crude Protein ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Ammonia Nitrogen ◽  
Neutral Detergent Fiber ◽  
Net Energy ◽  
Protein Concentrate ◽  
Dietary Nitrogen

Ten multiparous Holstein cows were used in a 105-day continuous feeding trial to compare soybean meal (SBM) and alfalfa protein concentrate (APC) as supplemental protein sources for high-producing dairy cows. Dairy cows with an average milk production of 35.2 kg/day were paired and randomly assigned to one of the treatments. A double reversal design was employed With 35 days per period. The comparison between treatments was made during the last 21 days of each period. Experimental diets, containing 60% corn silage and 10% alfalfa hay, were formulated to be isonitrogenous and isoenergetic (crude protein. 15.6%; net energy tor lactation, 1.60 Mcal/kg DM). Fifty percent of dietary nitrogen was provided by test proteins. Animals were fed four times daily. Chromie oxide boluses, 50 g/day were used to estimate digestion coefficients. Ruminal protein degradability in viva (%) and in situ(%)were 56.8, 68.3 and 47.3. 35.3 for SBM and APC, respectively. Dry matter intake (kg/day) was 19.1 and 18.6 for cows fed SBM and APC. respectively. Rummal parameters cows fed SBM vs APC were, pH. 6.6 vs 6.7, ammonia nitrogen(mg/dI) 10.8 vs 6.2(p less than 0.01); total volatile fatty acids (mM), 86.3 VS 84.6(P LESS THAN 0.05), acetate (%) , 50.8 vs 64.9 (p less than 0.01); propionate dry matter , 65.3 vs 61.8; crude protein . 67.6 vs 61.1 (P less than 0.05 ) neutral detergent fiber , 51.1 vs 52.5 ; acid detergent fiber, 47.7 vs 49.0 . A slightly higher (P more than 0.05 ) amount of milk was produced by cows fed APC(28.6+_ 3.5 vs 28.2+- 3.8 kg/day). Each additional gramof undergradable intake protein provided by APC continued to a 5- gram increase in 4% fat-corrected milk production.

scholarly journals In vitro and in vivo nutrient digestibility in sheep of rations with and without residue from the extraction of tamarind pulp

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4507
Author(s):  
Luiz Juliano Valério Geron ◽  
Fabiana Gomes da Costa ◽  
Silvia Cristina de Aguiar ◽  
Jocilaine Garcia ◽  
Matheus Gonçalves Ribeiro ◽  
...  
Keyword(s):  
Crude Protein ◽  
Ammonia Nitrogen ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber ◽  
Ruminal Fluid ◽  
Ph Values

<p>This study evaluated the digestibility of nutrients by, and parameters associated with, <em>in vitro </em>fermentation using different inocula (sheep ruminal fluid and feces) as well as the <em>in vivo </em>digestibility in sheep that were fed rations with 50% concentrate containing either no (0%) residue from the extraction of tamarind pulp (RETP) or 15% RETP. To determine the <em>in vitro </em>digestibility (<em>IV</em>D) of nutrients, two sheep, weighing 40.38 ± 2.10 kg, were used as inoculum donors. To determine the <em>in vivo </em>digestibility of nutrients, we used four sheep and a 3×2 factorial experimental design, with three methods of digestion of nutrients and two experimental rations (0% and 15% RETP). The variables were subjected to analysis of variance and the variables that showed differences at 5% probability were further analyzed using the Tukey test at 5% significance. The <em>IV</em>D using different inocula did not significantly differ (p&gt;0.05) from the <em>in vivo </em>digestibility in sheep for dry matter (DM), organic matter (OM), and neutral detergent fiber (NDF). The different methods for determining nutrient digestibility did not affect (p&gt;0.05) the digestibility of DM, OM, crude protein (CP), NDF, and acid detergent fiber (ADF) in rations with 0% and 15% RETP. However, the <em>IV</em>D of CP for rations containing 0% and 15% RETP incubated with both inocula was lower (p&lt;0.05) than the CP digestibility <em>in vivo</em>. The <em>in vivo </em>digestibility of ADF for rations containing 0% and 15% RETP was higher (P&lt;0.05) than the <em>IV</em>D using sheep ruminal fluid and feces as inocula. The pH values and concentration of ammonia nitrogen (NH3-N) after <em>in vitro </em>incubation for 24 h and the <em>in vivo </em>assay were not different (p&gt;0.05) for the rations containing 0% and 15% RETP, but the pH and NH3-N of both fermented and rumen contents differed (p&lt;0.05) depending on the inocula used and the <em>in vivo </em>assay. In summary, the digestibility of DM, OM, and NDF can be determined by the <em>in vitro </em>fermentation method using the ruminal fluid or feces of sheep as inocula in rations containing 0% or 15% RETP. However, <em>in vitro </em>fermentation is not a suitable method for the determination of pH and NH3-N concentration.</p>

DIGESTION OF CORN AND ALFALFA SILAGES IN THE STOMACH AND SMALL INTESTINE OF SHEEP PREPARED WITH TWO RE-ENTRANT CANNULAS

1981 ◽  
Vol 61 (1) ◽  
pp. 113-119 ◽  
Author(s):  
D. M. VEIRA ◽  
M. IVAN
Keyword(s):  
Organic Matter ◽  
Small Intestine ◽  
Formic Acid ◽  
Crude Protein ◽  
Ammonia Nitrogen ◽  
Weight Basis ◽  
Corn Silage ◽  
Fresh Weight ◽  
Fresh Weight Basis ◽  
Alfalfa Silage

Four wethers, each fitted with a re-entrant cannula in the proximal duodenum and terminal ileum, were used to study digestion of corn and alfalfa silages. The four silages fed were (1) corn silage (2) corn silage with 0.6% urea (fresh weight basis) added at the time of ensiling (3) direct-cut alfalfa silage treated with formic acid (5 g/kg fresh alfalfa) and (4) wilted alfalfa silage. Urea additions to corn silage increased crude protein from 8.1 to 13.2% which resulted in an increase (P < 0.05) in microbial yield from 1.99 to 3.23 g N/100 g organic matter (OM) apparently digested in the stomach. The apparent digestion of OM in the stomach was lower (P < 0.05) with formic-acid-treated alfalfa silage (48.6%) compared to the wilted alfalfa silage (56.1%), but this difference had disappeared at the ileum. The preservation of alfalfa silage with formic acid compared to wilting resulted in a greater (P < 0.05) flow from the stomach and digestion within the small intestine of non-ammonia nitrogen. This effect of formic acid on non-ammonia dynamics was unrelated to microbial yield.

scholarly journals Chemical composition of elephant grass silages supplemented with different levels of dehydrated cashew bagasse

2016 ◽  
Vol 37 (2) ◽  
pp. 997 ◽  
Author(s):  
Danillo Glaydson Farias Guerra ◽  
Isaac Sydney Alves da Silva Maia ◽  
Alexandre Paula Braga ◽  
Liz Carolina da Silva Lagos Cortes Assis ◽  
Jesane Alves de Lucena ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Crude Protein ◽  
Linear Growth ◽  
Neutral Detergent Fiber ◽  
Elephant Grass ◽  
Total Carbohydrate ◽  
Nitrogen Levels ◽  
Total Carbohydrate Content ◽  
Randomized Design ◽  
Different Levels

The objective of the present study was to evaluate the chemical composition of elephant grass silages supplemented with different levels dried cashew bagasse (DCB). Our experiment used a randomized design replicated four times, each replicate consisting of the following five treatments: 100% elephant grass; 95% elephant grass + 5% DCB; 90% elephant grass + 10% DCB; 85% elephant grass + 15% DCB; and 80% elephant grass + 20% DCB. The elephant grass was cut manually to a residual height of 5 cm at 80 days of age, and cashew bagasse was obtained from the processing of cashew stalks used in fruit pulp manufacturing in Mossoró/RN. Plastic buckets were used as experimental silos, and 90 days after ensiling the experimental silos were opened and the contents analyzed. The addition of dried cashew bagasse to silage linearly increased the levels of dried matter and crude protein by 0.59% and 0.13%, respectively, for each 1% addition (P < 0.05). The neutral detergent fiber and acid detergent content of the silages was reduced by 0.22% and 0.09%, respectively, for each 1% addition of the bagasse. The total carbohydrate content was not influenced by the bagasse addition (P > 0.05), and averaged 82.29%. The levels of non-fiber carbohydrate showed linear growth (P < 0.05) as the dehydrated cashew bagasse was added, and pH and ammoniacal nitrogen levels were reduced. The addition of the dehydrated bagasse to elephant grass silage improves its chemical composition, and it can be effectively added up to the level of 20%.

scholarly journals Effects of humic substances with urea on protozoal population and fermentation in the rumen of sheep

2020 ◽  
Vol 23 (1) ◽  
pp. 60-69
Author(s):  
A. Marcin ◽  
L. Bujňák ◽  
T. Mihok ◽  
P. Nad
Keyword(s):  
Humic Substances ◽  
Crude Protein ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Daily Intake ◽  
Crude Fibre ◽  
Merino Sheep ◽  
Positive Effects ◽  
Ph Values ◽  
And Control

The aim was to investigate the effects of humic substances (HS) combined with urea, on pH, ammonia, total volatile fatty acids (∑VFA), protozoal population, amylolytic and cellulolytic activities in rumen fluid (RF) of 12 female crossbred merino sheep (n = 6 in experimental EG and control CG groups). The basic daily ration consisted of 1.25 kg grass hay, 0.25 kg cereal grains – equally wheat and barley (daily intake of crude protein 113.45 g, crude fibre 394.19 g, crude fat 17.27 g and ash 71.85 g) and 10 g urea. HS were applied at 20 g.day-1 per animal in EG on day 2–18. Increased protozoal cells (P<0.05) of Entodinium spp. by 243.19, of Diplodinium spp. by 5.11 and of Ophryoscolex spp. by 2.06 103.mL-1 were observed in EG on day 3. The effects on the amylolytic and cellulolytic activities as well as pH values were not significant. Ammonia was higher (P<0.05) in EG by 5.78 at 3 h and by 12.75 at 6 h on day 2, by 10.62 at 9 h on day 3, and by 7.84 mg.100 mL-1 at 6 h on day 4 after feeding. ∑VFA were increased (P<0.05) by 3.89 mmol.L-1 at 3 h on day 18 after feeding. The feed intake of HS combined with urea had significantly positive effects on the course of ammonia and protozoal population in RF.

scholarly journals Effects of garlic supplementation on in vitro nutrient digestibility, rumen fermentation, and gas production

2021 ◽  
Vol 51 (2) ◽  
pp. 271-279
Author(s):  
M.R. Kekana ◽  
D. Luseba ◽  
M.C. Muyu
Keyword(s):  
Fatty Acids ◽  
Crude Protein ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Ammonia Nitrogen ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Nutrient Digestibility ◽  
Garlic Powder

Garlic contains secondary metabolites with antimicrobial properties that can alter nutrient digestibility and rumen fermentation, similar to other antimicrobial products. The objectives of the study were to evaluate the effects of garlic powder and garlic juice on in vitro nutrient digestibility, rumen fermentation, and gas production. The treatments consisted of control with no additives, garlic powder, and garlic juice at 0.5 ml and 1 ml. The digestibility of dry matter, crude protein and neutral detergent fibre were determined after 48 hours incubation. Rumen ammonia nitrogen and volatile fatty acids were determined at 12 hours and 24 hours incubation. The cumulative gas production was recorded periodically over 48 hours. The in vitro dry matter disappearance decreased with 1 ml of garlic juice compared with control. The crude protein degradability in garlic powder and garlic juice was lower than in control. Volatile fatty acids increased in all treatments. Individual volatile fatty acids were significantly different, especially propionate, whereas the acetate to propionate ratio was reduced by garlic juice, and ammonia nitrogen was reduced by garlic powder and 0.5 ml of garlic juice. The cumulative gas production increased significantly with both levels of garlic juice. The addition of garlic juice at 0.5 mL/100 ml could enhance the production of propionate, and reduce the acetate to propionate ratio, implying that the supply of hydrogen for methanogens was limited.

scholarly journals PROTEIN FRACTIONATION AND UTILIZATION OF SOYBEAN AND REDBEAN AS AFFECTED BY DIFFERENT DRYING TEMPERATURE

2017 ◽  
Vol 41 (1) ◽  
pp. 37
Author(s):  
Anuraga Jayanegara ◽  
Yesi Chwenta Sari ◽  
Roni Ridwan ◽  
Didid Diapari ◽  
Erika Budiarti Laconi
Keyword(s):  
Chemical Composition ◽  
Crude Protein ◽  
Volatile Fatty Acids ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Vitro Assay ◽  
Drying Temperature ◽  
Higher Temperature ◽  
In Vitro Rumen Fermentation

The objective of this study was to investigate the influence of different drying temperature on chemical composition, in vitro rumen fermentation and digestibility of soybean and redbean. Soybean and redbean were dried in an oven set at four different drying temperatures, i.e. 50, 60, 70 and 80 oC for 24 h in three replicates. Dried samples were then milled and used further for chemical composition determination (proximate analysis, Van Soest analysis and protein fraction) and in vitro rumen fermentation assay. Parameters measured in the in vitro assay were gas production, digestibility, pH, ammonia and volatile fatty acids (VFA). Data obtained were analyzed by using analysis of variance and a posthoc test namely Duncan’s multiple range test. Results revealed that neutral detergent insoluble crude protein (NDICP) content increased at higher drying temperature (70 or 80 oC) for both soybean and redbean (P<0.05) but at different magnitude. As with NDICP, higher temperature led to a higher acid detergent insoluble crude protein (ADICP) both in soybean and redbean (P<0.05). Higher temperature decreased gas production rate (GPR) of both beans (P<0.05). Drying of soybean at 70 or 80 oC decreased crude protein digestibility (CPD) of soybean than dried at 50 or 60 oC (P<0.05). Higher drying temperature resulted in a lower NH3 concentration (P<0.05). It can be concluded that drying temperature at 50 or 60 oC is safe to maintain nutritional quality of soybean and redbean.

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