scholarly journals COMPARATIVE EVALUATION OF ANTIBACTERIAL EFFICACY OF CHLOROQUICKONE STEP IRRIGATING SOLUTION AND CHLORHEXIDINE GLUCONATE SOLUTION ON E. FAECALIS IN ROOT CANAL SYSTEM OF PRIMARY MOLARS: A RANDOMISED EX VIVO STUDY.

2020 ◽  
pp. 1-3
Author(s):  
Shah Paridhi S ◽  
Dave Bhavna H
Keyword(s):  
Root Canal ◽  
Ex Vivo ◽  
Chlorhexidine Gluconate ◽  
Canal System ◽  
Qrt Pcr ◽  
Group I ◽  
Colony Forming Units ◽  
Root Canal System ◽  
Group Ii ◽  
Ex Vivo Study

Background: Microorganisms and their by-products are considered to be primary etiologic agents in endodontic diseases. Mechanical preparation is the principal mode of reducing the bacterial population in the root canal system, smear layer is still left on the root canal wall. Hence, irrigation of root canal allows for cleaning beyond what might be achieved by root canal instrumentation allows. Aim: To evaluate and compare antibacterial efficacy of Chloroquick- one step irrigating solution and 2% Chlorhexidine Gluconate on E. faecalis in primary molars by Quantitative Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR) study. Methodology: 40 children between age 4-7 years were included in this ex- vivo study based on selection criteria. In Group I (n=20) irrigation was done with chloroquick and normal saline and in Group II (n=20) irrigation was done with 2% chlorhexidine gluconate and normal saline. Pre- and post-irrigation samples were collected using sterile paper points and transferred into transporting medium in Eppendorf tube and sent for qRT-PCR analysis. Results: On intra-group comparison, in Group I a statistically significant reduction in E. Faecalis (colony forming units/ml) count post-irrigation was found (p=0.0130). While for Group II, there was no statistically significant reduction in E. Faecalis (colony forming units/ml) count post-irrigation (p=0.8180). On inter-group comparison between, Group I showed statistically significant reduction in E. Faecalis (colony forming units/ml) count as compared to Group II (p=0.0110) Conclusion: In the present study, continuous soft chelating irrigation protocol has shown promising results for reduction of microbial load from the root canal system of primary teeth

scholarly journals Photoactivated disinfection (PAD) of dental root canal system – An ex-vivo study

2016 ◽  
Vol 23 (1) ◽  
pp. 122-127 ◽  
Author(s):  
Dennis Mohan ◽  
Sunith Maruthingal ◽  
Rajamani Indira ◽  
Darshan Devang Divakar ◽  
Abdulaziz Abdullah Al Kheraif ◽  
...  
Keyword(s):  
Root Canal ◽  
Ex Vivo ◽  
Canal System ◽  
Root Canal System ◽  
Ex Vivo Study

An Ex Vivo Study of Root Canal System Configuration and Morphology of 115 Maxillary First Premolars

2020 ◽  
Vol 46 (6) ◽  
pp. 794-800 ◽  
Author(s):  
Thomas Gerhard Wolf ◽  
Christoph Kozaczek ◽  
Mark Siegrist ◽  
Madlena Betthäuser ◽  
Frank Paqué ◽  
...  
Keyword(s):  
Root Canal ◽  
Ex Vivo ◽  
System Configuration ◽  
Canal System ◽  
Root Canal System ◽  
Ex Vivo Study

scholarly journals Root canal debridement efficacy of heated sodium hypochlorite in conjunction with passive ultrasonic agitation: An ex vivo study

2020 ◽  
Vol 14 (4) ◽  
pp. 235-238
Author(s):  
Yogesh Damade ◽  
Ramchandra Kabir ◽  
Sunanda Gaddalay ◽  
Sharvaree Deshpande ◽  
Sonali Gite ◽  
...  
Keyword(s):  
Sodium Hypochlorite ◽  
Root Canal ◽  
Ethylenediaminetetraacetic Acid ◽  
Ex Vivo ◽  
Canal System ◽  
Ultrasonic Agitation ◽  
Root Canal System ◽  
Post Hoc ◽  
Root Canal Instrumentation ◽  
Ex Vivo Study

Background. This study aimed to investigate the endodontic debridement efficacy of different sodium hypochlorite (NaOCl) irrigation regimens with and without ultrasonic agitation, followed by ethylenediaminetetraacetic acid (EDTA) via scanning electron microscopy (SEM) after using a rotary instrumentation system. Methods. Mandibular premolars (n=50) were randomly divided into five experimental groups (n=10) for root canal instrumentation with ProTaper Universal rotary system up to F3. The root canal system was treated with intracanal-heated NaOCl (100°C) or preheated NaOCl (55°C), followed by ultrasonic agitation and EDTA treatment. Samples irrigated with conventional needle irrigation (CNI) using normal saline solution were used as controls. Debridement efficacy was analyzed by SEM. A five-point scale was used to estimate the presence/absence of debris for each canal segment (coronal, middle, and apical). The results were analyzed using one-way ANOVA and post hoc Tukey tests (P<0.05). Results. The experimental groups exhibited less debris compared to CNI with saline (P<0.05). The amount of debris decreased significantly for the group with NaOCl intracanal heating compared to extraoral heating. Ultrasonic agitation further enhanced the root canal debridement efficacy of NaOCl. Conclusion. In summary, intracanal heating of NaOCl with and without ultrasonic agitation followed by EDTA appears to be a promising method to flush debris from the root canal system.

Efficacy of ultrasonic and Er:YAG laser activated EDTA irrigation in removing bacteria from ex vivo root canal system

2018 ◽  
Vol 2 (4) ◽  
pp. 265-269
Author(s):  
Sharonit Sahar-Helft ◽  
Ayşe Sena Kabaş Sarp ◽  
Murat Gulsoy ◽  
Idan Redenski ◽  
Adam Stabholtz ◽  
...  
Keyword(s):  
Root Canal ◽  
Er:Yag Laser ◽  
Ex Vivo ◽  
Canal System ◽  
Root Canal System

scholarly journals Evaluation of Interference of Calcium Hydroxide-based Intracanal Medication in Filling Root Canal Systems

2011 ◽  
Vol 12 (5) ◽  
pp. 368-371 ◽  
Author(s):  
Matheus Melo Pithon ◽  
Deyla Duarte Vilela ◽  
Manoel Matos Neto ◽  
Alexandre Mascarenhas Villela
Keyword(s):  
Calcium Hydroxide ◽  
Clinical Significance ◽  
Root Canal ◽  
Canal System ◽  
Root Canals ◽  
Anterior Teeth ◽  
Knowing That ◽  
Group I ◽  
Canal Systems ◽  
Root Canal System

ABSTRACT Aim To evaluate the interference of the intracanal medication Calen® (SSWhite, São Paulo, Brazil) on the filling of simulated lateral canals. Materials and methods Twenty human anterior teeth were used. Before the endodontic filling procedures the access of cavity was made, and after this root canals were made in all the teeth to simulate the presence of lateral canals. After preparation, the teeth were randomly divided into two groups (n=10). In group I, the root canal system was filled directly after chemicalmechanical preparation; in group II, endodontic treatment was performed in multiple sessions, and after preparation the calcium hydroxide-based intracanal medication Calen® was inserted. After the period of 7 days, the root canals were vigorously irrigated and then they were filled. Next, the teeth were radiographed to verify the quality of the filling. Results The results demonstrated that the teeth treated in a single session, without calcium hydroxide medication, presented 47 canals out of 60 with radiographic evidence of filling, whereas the teeth in which intracanal medication was used, only 07 presented a radiographic image compatible with filling (p < 0.05). Conclusion The use of the calcium hydroxide-based medication Calen made it difficult to obtain a hermetic filling of the root canal system. Clinical significance The clinical significance of this work basing on the fact that once the dentist knowing that property obliteration of calcium hydroxide can be taken care when they are used in the presence of lateral canals. How to cite this article Vilela DD, Neto MM, Villela AM, Pithon MM. Evaluation of Interference of Calcium Hydroxide-based Intracanal Medication in Filling Root Canal Systems . J Contemp Dent Pract 2011;12(5):368-371.

scholarly journals The Efficacy of Selected Sodium Hypochlorite Heating Methods for Increasing and Maintaining Its Intracanal Temperature—An Ex Vivo Study

2022 ◽  
Vol 12 (2) ◽  
pp. 891
Author(s):  
Roberto De Santis ◽  
Flavia Iaculli ◽  
Vincenzo Lodato ◽  
Vito Gallicchio ◽  
Michele Simeone ◽  
...  
Keyword(s):  
Sodium Hypochlorite ◽  
Root Canal ◽  
Temperature Increase ◽  
Ex Vivo ◽  
Canal System ◽  
Heating Method ◽  
In Vitro Investigation ◽  
Significant Temperature ◽  
Root Canal System ◽  
Different Levels

Background: Enhancement of the temperature of sodium hypochlorite (NaOCl) solution would increase its cleaning potential and decontamination of the root canal system. Therefore, the aim of the present in vitro investigation was to compare the efficacy of different methods of NaOCl heating by evaluating the temperature profiles developed at different levels of the root canal system. Methods: Five thermocouples were applied at different levels of the root canal system of extracted human premolars. NaOCl solution was heated according to two methods: extraoral heating (50 °C, 60 °C, and 70 °C) using a magnetic hotplate heater and intracanal heating by F-06, XF-30/04, and ML-12 pluggers at 100 °C, 150 °C, and 180 °C. Results: The extraoral heating method was ineffective to produce a significant temperature increase at the root apex. Comparable results were obtained using the intracanal heating method through the ML-12 plugger that showed slightly better results only when set at 180 °C. On the other hand, negligible differences were observed in terms of temperature maintenance at several levels of the root between the F-06 and XF-30/04 pluggers, even though the time intervals were higher in case of XF-30/04. Conclusions: The intracanal heating method provided a better temperature persistence in the middle third of the root canal system. Conversely, extraoral heating was ineffective to produce a significant temperature increase at the apex of the root. Comparable results were obtained even using the ML-12 plugger.

scholarly journals COMPARATIVE EFFECTIVENESS OF TREATMENT OF CHRONIC APICAL PERIODONTITIS ON THE BASIS OF DENSITOMETRIC AND MICROBIOLOGICAL RESEARCH METHODS

2019 ◽  
Vol 15 (2) ◽  
pp. 17-24 ◽  
Author(s):  
Лариса Герасимова ◽  
Larisa Gerasimova ◽  
Алина Юсупова ◽  
Alina Yusupova ◽  
Ирина Усманова ◽  
...  
Keyword(s):  
Diode Laser ◽  
Root Canal ◽  
Laboratory Data ◽  
Apical Periodontitis ◽  
Endodontic Treatment ◽  
Canal System ◽  
Periodontal Tissues ◽  
Complex Anatomy ◽  
Group I ◽  
Root Canal System

Relevanct of the research topic. One of the possible causes of apical periodontitis is the infectious inflammatory process of the periapical periodontal tissues. The microflora of the root canal system is represented by various microorganisms, among which streptococci, enterococci and staphylococci most often prevail. The complex anatomy of the root canal system ensures the growth and reproduction of microorganisms, and the endodontic treatment does not always contribute to their qualitative and quantitative reduction. Purpose ― study of the effectiveness of endodontic treatment of chronic apical periodontitis in the near and long term. Methods. We observed 110 patients aged 20 to 30 years with apical periodontitis, bone density analysis and microbiological examination of the contents of the root canal. Depending on the obtained clinical and laboratory data, the patients were divided into two clinical groups: in group I endodontic treatment of 55 teeth was carried out according to the scheme developed by us; in group II — 55 teeth using the standard method of treatment. Results. In endodontic treatment of chronic apical periodontitis, treatment of the root canal system with a diode laser promotes better decontamination of the microflora. Coal mining introduction into the root canal system and in the region of the transition folds of the platelet autoplasma leads to an increase of the reparative process in the periapical region (p≤0.05). Summary. The inclusion in the Protocol of endodontic treatment of apical periodontitis of treatment of the root canal system with a diode laser and injection of platelet autoplasm is a more effective method of treatment in comparison with the traditional one.

scholarly journals Comparative Evaluation of the Efficacy of Irrigation Delivery Systems on Enterococcus faecalis

2018 ◽  
Vol 25 (3) ◽  
Author(s):  
Raksha Bhat ◽  
◽  
Preethesh Shetty ◽  
Mithra N. Hegde ◽  
◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Delivery Systems ◽  
Semiconductor Diode ◽  
Health Administration ◽  
Canal System ◽  
Group I ◽  
Root Canal System

Attaining a sterile root canal system is important since microorganisms can cause persistent inflammation in the periradicular tissues. Objectives: This study sought to evaluate the efficacy of two irrigation delivery systems; the EndoVac and semiconductor diode laser irradiation with 5.25% sodium hypochlorite and 2% chlorhexidine. Methods: Fifty teeth were disinfected according to the Occupational Safety and Health Administration regulations. The teeth were then instrumented, followed by inoculation with bacterial strains of Enterococcus faecalis (ATCC 29212). The teeth were randomly assigned to five groups, Group I: Control group; Group II: Teeth irrigated with 5.25% NaOCl + Endovac; Group III: Teeth irrigated with 2% Chlorhexidine + Endovac; Group IV: Teeth irrigated with 5.25% NaOCl + laser irradiation; and Group V: Teeth irrigated with 2% Chlorhexidine laser irradiation. All samples were incubated on Muller–Hilton media plates for a period of 24 h. We determined the colony-forming units and analyzed them statistically using Fisher’s exact test. Results: Laser irradiation completely disinfected the root canal system. The EndoVac system produced significant disinfection but was comparatively less effective than laser irradiation. Conclusion: Laser irradiation had a significantly greater bactericidal effect than the EndoVac system, used in conjunction with sodium hypochlorite and chlorhexidine.

scholarly journals Effect of Passive Ultrasonic Irrigation on Enterococcus faecalis from Root Canals: An Ex Vivo Study

2015 ◽  
Vol 26 (4) ◽  
pp. 342-346 ◽  
Author(s):  
Juliane Maria Guerreiro-Tanomaru ◽  
Gisselle Moraima Chávez-Andrade ◽  
Norberto Batista de Faria-Júnior ◽  
Evandro Watanabe ◽  
Mário Tanomaru-Filho
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Saline Solution ◽  
Ex Vivo ◽  
Canal System ◽  
Passive Ultrasonic Irrigation ◽  
Root Canals ◽  
Significant Difference ◽  
Ultrasonic Irrigation ◽  
Root Canal System

<p>Endodontic irrigation aims to clean and disinfect the root canal system. Passive ultrasonic irrigation (PUI) is based on the use of an ultrasound-activated instrument into the root canal filled with irrigant. The aim of this study was to evaluate, ex vivo, the effectiveness of PUI in eliminating <italic>Enterococcus faecalis</italic> from root canals. Seventy-five extracted human single-root teeth were used. After root canal preparation, specimens were inoculated with <italic>E. faecalis</italic> and incubated at 37 °C for 21 days. Specimens were distributed into five groups (n=15), according to the irrigation method: PUI + saline solution (PUI/SS); PUI + 1% NaOCl (PUI/NaOCl); conventional needle irrigation (CNI) + saline solution (CNI/SS); CNI + 1% NaOCl (CNI/NaOCl); No irrigation (control). Microbiological samples were collected at three time points: initial (21 days after inoculation), post-irrigation (immediately after irrigation), and final (7 days after irrigation). Data were obtained in CFU mL<sup>-1</sup> and subjected to analysis by ANOVA and Tukey's tests at 5% significance level. The post-irrigation samples did not demonstrate statistical difference between PUI/SS and CNI/SS nor between PUI/NaOCl and CNI/NaOCl (p>0.05), but PUI/NaOCl and CNI/NaOCl had lower CFU mL<sup>-1</sup> number than the other groups (p>0.05). Statistically significant difference was observed between the initial and post-irrigation samples and between the post-irrigation and final samples (p<0.05) in all groups, except in the control. The final samples of all groups presented bacterial counts similar to the initial samples. PUI or CNI with 1% NaOCl contribute to disinfection, but are unable to eradicate <italic>E. faecalis</italic> from the root canal system.</p>

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