scholarly journals Effect of harvest time and fruit size on seed germination and seedlings growth of sour orange and Mexican lime under in vitro conditions

2021 ◽  
Vol 35 (2) ◽  
Author(s):  
Sakineh Jokari ◽  
Akhtar Shekafandeh
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Fruit Size ◽  
Dry Weight ◽  
Germination Percentage ◽  
Sour Orange ◽  
Mexican Lime ◽  
Randomized Design ◽  
Fruit Harvest

The aim of this research was to determine the best time to harvest the fruits for seed production which would ultimately lead to the production of citrus rootstocks of optimum quality. The sour orange and Mexican lime fruits were harvested on 7 and 5 occasions, respectively. The very first fruits were harvested 80 days after flowering and subsequent harvests were gathered every 30 days. An in vitro experiment was carried out in a completely randomized design, with four replications and 20 seeds in each replication. Based on fruit growth curve the time of fruit harvest affected seed germination (percentage and rate) and seedling growth (stem and root length, fresh and dry weight of stems, roots and leaves).  The results showed that the best time to harvest the fruits of sour orange and Mexican lime was 230 and 170 days after flowering, respectively, which led to maximum seed germination (Mexican lime 100% and sour orange 85%) and seedling growth. The highest root, stem and leaf fresh and dry weight was also obtained at 230 and 170 days after flowering in sour orange and Mexican lime respectively.   

scholarly journals SEED GERMINATION AND RADICLE ESTABLISHMENT RELATED TO TYPE AND LEVEL OF SALT IN COMMON BEAN (PHASEOLUS VULGARIS L. VAR. DJEDIDA)

2019 ◽  
Vol 52 (3) ◽  
pp. 262-277
Author(s):  
L.M. MANSOURI ◽  
N. HELEILI ◽  
Z.F. BOUKHATEM ◽  
A. KHELOUFI
Keyword(s):  
Phaseolus Vulgaris ◽  
Seed Germination ◽  
Common Bean ◽  
Seedling Growth ◽  
Dry Weight ◽  
Germination Percentage ◽  
Phaseolus Vulgaris L ◽  
Randomized Design ◽  
Dry Biomass ◽  
Secondary Roots

Saline soils contain multiple types of salt, each of them may exert a different effect on seed germination and seedling growth. The aim of this study is to assess the effects of five types of salt on the seed germination and radicle establishment of common bean (Phaseolus vulgaris L. var. Djedida). The experiment was set out as a factorial experiment based on completely randomized design with four replications of ten seeds for the seed germination and twelve replicates for the seedling growth. We used five kinds of salts (NaCl, Na2SO4, CaCl2, CaCO3 and KCl) with concentrations of 0, 100, 200 and 300 mM. Seeds were incubated in Petri dishes at 25°C, in the dark, for 10 days. The results of analysis of variance indicated that the effects of salt types and concentrations, and their interaction effect were significant in all measured traits (P< 0.01). However, no significance effect was found on secondary roots number and seedlings dry biomass with CaCO3 treatment solution. According to the results, the inhibitory effects of the five salt types differed significantly. Indeed, germination of common bean seeds by various salts were in the order of NaCl > KCl > CaCO3 > Na2SO4 > CaCl2. However, the taproot length, the number of secondary roots and the seedlings dry weight by various salts were in the order of CaCO3 > KCl > NaCl > CaCl2 > Na2SO4. The effect of salt concentration was also obvious. The reduction in dry biomass of cotyledons is proportional to germination rates and to the development of seedlings in dry biomass and in size. Seeds of P. vulgaris var. Djedida were able to germinate under all concentrations of the various types of salt. The lowest final germination percentage (FGP) was obtained under 300 mM of all salts recording the following values: CaCO3 - 60%, NaCl - 60%, KCl - 52.5%, Na2SO4 - 50% and CaCl2 - 27.5%. During germination stage, the radicle emergence would be controlled by the environment osmolarity, while the later growth of the seedling would be limited by the reserve mobilization.

The Effects of Salinity Stress on Seed Reserve Utilization and Germination Percentage of Treated Seeds of Barley (Hordeum Vulgare L.)

2014 ◽  
Vol 47 (1) ◽  
pp. 23-29
Author(s):  
S.A. Tabatabaei
Keyword(s):  
Hordeum Vulgare ◽  
Salinity Stress ◽  
Seedling Growth ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Utilization Efficiency ◽  
Hordeum Vulgare L ◽  
Randomized Design ◽  
Percentage Weight

ABSTRACT In order to investigate salinity stress on seed reserve utilization and seedling growth of treated seeds of barley (Hordeum vulgare L.), an experiment was carried out. Factorial experiment was carried out in completely randomized design with three replicates. To create salinity stress, NaCl in osmotic levels at 0 (as control), -4, -8, -12 and -16 bar were used. For seed priming, gibberellin (GA) 50 ppm was used. Our results showed that treatment × drought interaction on these traits: germination percentage, weight of utilized (mobilized) seed, seed reserve utilization efficiency, seedling dry weight and seed reserve depletion percentage were significant. The highest germination percentage, weight of utilized (mobilized) seed, seed reserve utilization efficiency, seedling dry weight and seed reserve depletion percentage were attained from priming by gibberellin at control conditions. Thus, priming increased characteristics as compared to the unprimed. Priming improved seed reserve utilization such as: weight of utilized (mobilized) seed reserve, seed reserve depletion percentage, seed reserve utilization efficiency and seedling growth in barley under salinity stress.

scholarly journals Effect of osmopriming on rice seed germination and seedling growth

2012 ◽  
Vol 10 (1) ◽  
pp. 15-20 ◽  
Author(s):  
R Islam ◽  
A Mukherjee ◽  
M Hossin
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Germination Percentage ◽  
Shoot Length ◽  
Rice Seed ◽  
Randomized Design ◽  
Vigor Index ◽  
Two Factors ◽  
Treated Seed ◽  
Germination Parameters

The experiment was conducted in the Agronomy Laboratory of Agrotechnology Discipline, Khulna University, Khulna from 29th August to 17th September, 2009. The experiment was laid out in a Completely Randomized Design (CRD) with two factors and three replications. The aim of the experiment was to study the efficacy of three osmopriming agents viz ., NaCl, KC1 and CaCb with control on seed germination and seedling growth of three T. aman rice varities viz., BRRI dhan40, BRRI dhan41 and BINA dhan7. Among the chemicals CaCb showed best as osmopriming agents and it enhanced all germination parameters. BRRI dhan41 showed the best performance. Better root length and shoot length were observed in control. Seeds of BRRI dhan41 when treated with NaCl showed the highest germination percentage (98.67%), germination energy (88%), germination speed (96.83%), but vigor index (10.63) was found highest in BRRI dhan40 when it was treated with KC1. BRRI dhan41 produced the largest root (8.56 cm) when seeds were treated with NaCl. BRRI dhan40 (10.51 cm) and BINA dhan7 (10.53 cm) produced the highest shoot length at controlled condition and CaCl2 treated seed, respectively.   DOI: http://dx.doi.org/10.3329/jbau.v10i1.12013   J. Bangladesh Agril. Univ. 10(1): 15–20, 2012  

scholarly journals Studies on Seed Germination, Seedling Growth, and In Vitro Shoot Induction of Aloe ferox Mill., a Commercially Important Species

HortScience ◽  
2009 ◽  
Vol 44 (3) ◽  
pp. 751-756 ◽  
Author(s):  
Michael W. Bairu ◽  
Manoj G. Kulkarni ◽  
Renée A. Street ◽  
Rofhiwa B. Mulaudzi ◽  
Johannes Van Staden
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Shoot Multiplication ◽  
Germination Percentage ◽  
Shoot Induction ◽  
Ex Vitro ◽  
Important Species ◽  
Aloe Ferox ◽  
Smoke Water

A study was done to investigate the effects of some physical and chemical factors on growth and development of Aloe ferox ex vitro and in vitro. The effects of light, temperature, and smoke–water on seed germination, ex vitro seedling growth requirements, and effect of germination medium and cytokinins on shoot induction and multiplication in vitro were investigated. The highest germination percentage of A. ferox seeds was recorded between 15 and 30 °C. Seed germination was inhibited at 10 and 35 °C. There was a nonsignificant increase in germination percentage (78%) for seeds that were germinated under constant dark conditions. Smoke–water-treated seeds showed significant improvement in percentage germination (80%) over the control (66%) at 25 °C with a 16-h photoperiod. Seedlings of A. ferox subjected to alternating temperatures (30/15 °C) or being irrigated three times weekly at 25 °C showed better seedling growth. In an in vitro experiment, seedlings germinated in distilled water and one-tenth strength Murashige and Skoog medium had superior shoot induction competence compared with the other germination media. The cytokinins meta-Topolin (mT) and meta-Topolin riboside (mTR) at 5 μm gave significantly higher shoot multiplication rates compared with the control and benzyladenine (BA)-treated plants. A higher abnormality index was recorded for BA-treated plants. The findings of this study will be beneficial for commercial propagation of Aloe ferox.

scholarly journals Seed Reserve Utilization and Seedling Growth of Treated Seeds of Mountain Rye (Secale Montanum) as Affected by Drought Stress

2012 ◽  
Vol 45 (2) ◽  
pp. 43-48 ◽  
Author(s):  
O. Ansari ◽  
H. Chogazardi ◽  
F. Sharifzadeh ◽  
H. Nazarli
Keyword(s):  
Drought Stress ◽  
Seedling Growth ◽  
Dry Weight ◽  
Germination Percentage ◽  
Utilization Efficiency ◽  
Significant Treatment ◽  
Field Crops ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Secale Montanum

Seed Reserve Utilization and Seedling Growth of Treated Seeds of Mountain Rye (Secale Montanum) as Affected by Drought StressThe environmental stress such as, drought are serious obstacles for field crops in further areas of the world, especially in arid and semiarid regions. In order to investigate drought stress on seed reserve utilization and seedling growth of treated seeds of mountain rye (Secale montanum), an experiment was carried out. Factorial experiment was carried out in completely randomized design with three replications. The first factor was the seed treatments (unpriming, hydropriming and osmopriming) and the second factor was drought stress. To create drought stress, polyetylenglycol 6000 (PEG 6000) in osmotic levels at 0 (as control), -0.4, -0.8, - 1.2 and -1.6 MPa were used. The results indicated that for these traits: germination percentage (GP), timson index (TI), energy of germination (EG), weight of utilized (mobilized) seed (WMSR), seed reserve utilization efficiency (SRUE), seedling dry weight (SLDW), and seed reserve depletion percentage (SRDP), was a significant treatment x drought interaction. Thus hydropriming and osmopriming improvement study traits inSecale montanumunder drought stress. While in higher osmotic pressure the highest seed reserve utilization were obtained from osmopriming.

scholarly journals Respon Viabilitas Benih Kacang Tunggak Nagara (Vigna unguiculata ssp cylindrica) Akibat Pemberian Konsentrasi Ekstrak Akar Eceng Gondok (Eichhornia crassipes)

2020 ◽  
Vol 10 (2) ◽  
pp. 63-73
Author(s):  
Raihani Wahdah ◽  
Hikma Ellya ◽  
Hasni Hairina
Keyword(s):  
Seed Germination ◽  
Water Hyacinth ◽  
Dry Weight ◽  
Germination Percentage ◽  
Growth Speed ◽  
Root Extract ◽  
Randomized Design ◽  
Normal Seedling ◽  
Physiology Laboratory ◽  
Water Hyacinth Root

       Water hyacinth root contains Giberilin which was expected to improve the viability performance of nagara cowpea.  The aim of this research was to study the effect of water hyacinth root extract on the viability  of nagara cowpea. The research was carry out in April - October 2020 at the Plant Physiology Laboratory, Faculty of Agriculture, Lambung Mangkurat University, Banjarbaru. This research was arranged in a single factor completely randomized design, namely the concentration of water hyacinth root extract (control, 0%, 7.5%, 15.0%, 22.5%, and 30.0%). Observations were made on seed germination, viability potential, percentage of normal seedling at first observation, growth speed, uniformity of growth, root and plumule length of strong normal seedling, and dry weight of normal seedling.. If the treatment has a significant effect, then proceed with DMRT (Duncan Multiple Range Test). The 0.0% priming was better on all variables than the without priming, except for the root length.  The 7.5% priming treatment was the most efficient for seed germination, potential germination of seeds, and  growth uniformity of seeds. The 22.5% priming treatment was the most efficient for the germination percentage in first observation, seed growth speed, plumule length, and dry weight of normal seedling.

scholarly journals Improving seed germination and seedling growth of true seed shallot (TSS) using plant growth regulator seed priming

2021 ◽  
Vol 883 (1) ◽  
pp. 012024
Author(s):  
R Pangestuti ◽  
E Sulistyaningsih ◽  
B Kurniasih ◽  
R H Murti
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Seedling Growth ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Experimental Unit ◽  
Significant Difference

Abstract Seeding is the most important and quite challenging stage in seed/TSS cultivation. Plant growth regulator (PGR) seed priming has the potential to enhance the quality of TSS seedlings. The research had been conducted in the greenhouse of Agriculture Faculty, UGM, from February to April 2020. The factorial treatments of shallot cultivars (Tuk-Tuk, Lokananta and Sanren) and seed treatments (soaking seed with GA3 100 ppm and NAA 50 ppm for 12 hours and untreated seeds as control) were assigned in the RCBD with four replications. Each experimental unit was consisted of 728 seeds per cultivar. Both treated and untreated seeds were sown in soil blocks. The data were analyzed using ANOVA and continued with Tukey HSD procedure at α=0.05. The results showed that PGR priming on seeds significantly increased the germination percentage, plant height, leaf number, leaf area, and hypothetical vigor index of the three cultivars at six weeks after sowing. However, there was no significant difference in the fresh weight and dry weight of seedlings. This research implied that soaking seeds in GA3 100 ppm and NAA 50 ppm for 12 hours could improve seed germination and TSS seedling growth. Additional fertilizers are perhaps needed in soil blocks to increase the effect of hormones on the seedlings.

scholarly journals Evaluation of allelopathic effect of fifty Sorghum bicolor L. Genotypes on Germination and Growth of some weed in vitro and in vivo

2010 ◽  
Vol 4 (1) ◽  
pp. 95-103
Author(s):  
A. A. Aljibouri ◽  
S. N. Mahmoud ◽  
A. W. Alsawani ◽  
I. S. Alsaadawi ◽  
Duha Mysire Majeed
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Dry Weight ◽  
Amaranthus Retroflexus ◽  
Allelopathic Effect ◽  
Whole Plant ◽  
Number Of Leaves ◽  
Sorghum Genotypes ◽  
Germination And Growth

The effect of root exudates of fifty Sorghum genotypes on seed germination and seedling growth of millet were studied in vitro. The fifty genotypes were divided into four groups according to their effect on percentage reduction in root and shoot and whole plant average lengths of millet seedling growth. The first group caused slight stimulation, the second slight inhibition, the third gave limited inhibition and the fourth caused high inhibition. In seedling growth. Five genotypes of sorghum were selected, one from group two and four from group four. These five genotypes were cultured in the field in Autumn 2007 and at the end of the season plants were removed, dried and grind. Three concentrations of each of these genotypes powder were prepared, in addition to the control treatment, (0.0, 3.0, 6.0 and 9.0) g/kg soil to study their effects on the growth of Amaranthus retroflexus which cultured in pots and kept in experimental field. Data on number of leaves, lengths of shoot and root and whole plant were taken as well as wet and dry weight were measured. Percentage of Amaranthus retroflexus seeds germination were evaluated in an experiment cultured in Petri dishes containing concentrations (0.0, 5.0 and 10.0%) of aqueous extracts of each of the five genotypes powder. Results showed high significant inhibition for the four genotypes from group four in comparison with the one genotype from group two. Significant differences among these genotypes in their inhibition on such parameters as shoot, root, whole plant growth, number of leaves, and wet and dry weight. The genotype 2005-K-Type 1050 gave the highest reduction in plant length 53.2% incomparing with the genotype 2005-K-Type 1033 which gave 0.7%. The percentage of seed germination of Amaranthus retroflexus was significantly reduced as the concentration of the aqueous extract increased and there were significant differences among genotypes on this parameter. The concentration of the powder of each genotype added to the soil produced significant reduction in all parameters studied and the effect increased as the concentration increased in soil. This research discusses the potential of the allelopathic effect of some Sorghum genotypes on weed germination and growth reduction and the possibility of employing them in weed control program with the intention to use less herbicide.

scholarly journals In vitro effect of seed bio-priming techniques on seed germination and seedling vigour of few vegetable crops

2020 ◽  
Vol 12 (4) ◽  
pp. 702-709
Author(s):  
Rakesh Kumar Jaiman ◽  
Sanjay Kumar Acharya ◽  
Naziya P. Pathan ◽  
Amol J. Deshmukh ◽  
Haresh A. Desai ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seed Treatment ◽  
Trichoderma Viride ◽  
Solanum Melongena ◽  
Dry Weight ◽  
Germination Percentage ◽  
Vegetable Crops ◽  
Seedling Vigour ◽  
Vitro Effect

Bio-priming can also be viewed as a new technique of seed treatment using biological agents to stimulate germination of seed and growth of the plant and further protecting the seed from soil-and seed-borne pathogens. The present investigation was carried out in vitro conditions on seed germination and seedling vigour of few vegetable crops viz.  tomato (Solanum lycopersicum L.), brinjal (Solanum melongena L.), onion (Allium cepa L.) and chilli (Capsicum annuum L.), during 2015 and 2016. The treatments comprised viz. T1: Non primed seeds (Control), T2: Seed treatment with Carbendazim 2.5g/kg seed, T3: Hydro-priming for 6 hrs, T4: Hydro-priming for 12 hrs, T5: Hydro-priming for 18 hrs, T6: Biopriming with Trichoderma  viride for 6 hrs, T7: Biopriming with T.viride for 12 hrs, T8: Biopriming with T.viride for 18 hrs, T9: Biopriming with Trichoderma harzianum for 6 hrs, T10: Biopriming with T. harzianum for  12 hrs, T11: Biopriming with T. harzianum for 18 hrs, T12: Biopriming with Pseudomonas fluorescens for 6 hrs, T13: Biopriming with P. fluorescens for 12 hrs and T14: Biopriming with P. fluorescens for 18 hrs.  The results revealed that maxiumum germination percentage (92.92, 90.77,83.00 and 86.33), seedling length (32.38 cm, 29.35 cm, 31.75 and 31.60 cm), seedling fresh weight (2.07 g, 4.01 g, 3.05 g and 2.04 g),  seedling dry weight (0.42 g, 0.86 g, 0.62 g and 0.42 g) and seedling vigour index (3008.11, 2664.00, 2635.00 and 2728.00) were recorded in T10 (bio priming with T. harzianum for 12 hrs) in tomato (S. lycopersicum L.), brinjal (S. melongena L.), onion (A. cepa L.) and chilli (C. annuum L.), respectively. Thus, it indicated that priming of seeds of these crops with T. harzianum/P. fluorescens/ T. viride  for 12 hrs was very effective with respect to their vegetative growth along with the quality yield.

scholarly journals Effects of Exhaust Pollutants and Garden Soil on Seed Germination and Seedling Growth of Maize (Zea mays L.) and Sunflower (Helianthus annuus L.)

2019 ◽  
pp. 1-7
Author(s):  
Namrah Zafar ◽  
Muhammad Zafar Iqbal ◽  
Muhammad Shafiq ◽  
Muhammad Kabir ◽  
Zia-ur-Rehman Farooqi
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Zea Mays L ◽  
Dry Weight ◽  
Soil Extract ◽  
Germination Percentage ◽  
Control Treatment ◽  
Garden Soil ◽  
Seedling Length ◽  
Standard Solution

Aim: This study was conducted to examine the effects of exhaust pollutants and garden soil extract on seed germination and seedling growth of some economically important crops, maize and sunflower. Study Design: The in-vitro experimental design was completely randomized. Place and Duration of Study: The experimental site was Department of Botany, University of Karachi, Pakistan and the experiment was lasted for 10 days in Ecology laboratory. Methodology: The seeds of maize and sunflower were placed in front of the generator to exposed exhaust pollutants daily, 10 minutes, for 10 days. After the exposure of exhaust gas seeds were shifted into Petri dishes. The garden soil was obtained from mini garden of the Department of Botany, University of Karachi. The different concentrations of garden soil with distilled water were made and filtered. This filtered solution was assumed as the standard solution, which was 100%. From this standard solution, further dilution of 25%, 50% and 75% were made. Distilled water was used as a control for the experiment. Three best seedlings were selected from each Petri dish for the determination of mean values of seed germination (percentage), root, shoot and seedling length and seedling dry weight.   Results: This publication will help to understand the importance of exhaust pollutant impact and soil on crop growth and productivity. The soil quality influences plant growth and identifies the impact of exhaust emission on plant growth. The effects of exhaust pollutants of the generator and different concentration of garden soil on seed germination and seedling growth of two important crops Zea mays (L.) maize and sunflower Helianthus annuus (L.) were recorded. The treatment of different concentration of garden soil extract (0, 25, 50, 75, and 100%) affected seed germination percentage, root, shoot, and seedling length and seedling dry weight of both crop species. The soil extract at 25% significantly (p<0.05) affected the seed germination percentage of H. annuus as compared to control. The treatment of soil extract also decreased seedling growth performance of Z. mays and H. annuus as compared to control. A significant (p<0.05) decrease in seedling length of Z. mays becomes evident that exhaust treatment affected the seedling dry weight performance of maize as compared to control. Similarly, exposure of exhaust pollutant affected the seedling dry weight of Z. mays and H. annuus as compared to control treatment.  The effects of garden soil extract on seed germination and seedling growth of Z. mays was found higher as compared H. annuus. Conclusion: It was concluded that the treatment of exhaust emissions from a portable power generator fueled negatively affected the seed germination and seedling growth performance of maize and sunflower as compared to control treatment. A comparison between the seed germination percentage, seedling growth and biomass production performance of maize was found highly affected as compared to sunflower.

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