scholarly journals Improving seed germination and seedling growth of true seed shallot (TSS) using plant growth regulator seed priming

2021 ◽  
Vol 883 (1) ◽  
pp. 012024
Author(s):  
R Pangestuti ◽  
E Sulistyaningsih ◽  
B Kurniasih ◽  
R H Murti

Abstract Seeding is the most important and quite challenging stage in seed/TSS cultivation. Plant growth regulator (PGR) seed priming has the potential to enhance the quality of TSS seedlings. The research had been conducted in the greenhouse of Agriculture Faculty, UGM, from February to April 2020. The factorial treatments of shallot cultivars (Tuk-Tuk, Lokananta and Sanren) and seed treatments (soaking seed with GA3 100 ppm and NAA 50 ppm for 12 hours and untreated seeds as control) were assigned in the RCBD with four replications. Each experimental unit was consisted of 728 seeds per cultivar. Both treated and untreated seeds were sown in soil blocks. The data were analyzed using ANOVA and continued with Tukey HSD procedure at α=0.05. The results showed that PGR priming on seeds significantly increased the germination percentage, plant height, leaf number, leaf area, and hypothetical vigor index of the three cultivars at six weeks after sowing. However, there was no significant difference in the fresh weight and dry weight of seedlings. This research implied that soaking seeds in GA3 100 ppm and NAA 50 ppm for 12 hours could improve seed germination and TSS seedling growth. Additional fertilizers are perhaps needed in soil blocks to increase the effect of hormones on the seedlings.

2016 ◽  
Vol 2 (2) ◽  
pp. 104
Author(s):  
Tyas Larasati ◽  
Suci Rahayu ◽  
Fauziyah Harahap

The objectives of this research were to composed organ from callus culture and to found the best concentration of plant growth regulator for organ growth from female flower explant of oil palm. This research has already done from June 2014 to May 2015 at Laboratory of Plant Physiology and Tissue Culture Department of Biology Faculty of Mathematics and Science University of North Sumatera. This research used Nonfactorial Completely Random Design. Explant was treated with five concentrations of 2,4-Dichlorophenoxy acetic acid (2,4-D; 99, 110, 120, 132, and 140 mg/L) for callus induction on Y3 medium (Eeuwens 1976). The result of this research showed that organ was formed from this treatment (basal segment of female flower explant) was root organ. 2,4-D plant growth regulator positively affected to growing of the root. The best result for time of callus induction, time of root growth, the highest percentage of explants that formed the root, fresh weight and dry weight of callus that has become the root generation was resulted from 99 mg/L 2,4-D.   Key words: Elaeis guineensis Jacq., female flower, plant growth regulator 2,4-D, organogenesis


1991 ◽  
Vol 116 (2) ◽  
pp. 191-200 ◽  
Author(s):  
E. M. White

SUMMARYApplications of nitrogen and a plant growth regulator (mepiquat chloride and ethephon) were used to manipulate stem structure and induce differing degrees of damage due to leaning and lodging in six cultivars of winter barley grown in Belfast, UK, in 1986/87. Weighted incidences of leaning and lodging were combined to give an index indicating damage susceptibility of the cultivars. The index was very high (70) in Pipkin and ranged between 1 and 18 in the other cultivars. Differences between cultivars in number of internodes, plant height and stem weight did not explain their differences in resistance to damage. However, dry weight per unit length ranged from 2·35 and 2·34 mg/mm in the strongest cultivars, Panda and Jennifer, respectively, to 1·75 mg/mm in the weakest cultivar, Pipkin.Nitrogen application increased plant height but did not affect dry weight/main stem, so that dry weight/unit length of stem decreased. The growth regulator treatments reduced plant height and although dry weight/stem did not decrease significantly, dry weight/unit length of stem was similar in treated and untreated plots.Dry weight/unit length has potential as an objective indicator of straw strength in winter barley cultivars and could be used in cultivar evaluation in the absence of damage in field trials.


2014 ◽  
Vol 47 (1) ◽  
pp. 23-29
Author(s):  
S.A. Tabatabaei

ABSTRACT In order to investigate salinity stress on seed reserve utilization and seedling growth of treated seeds of barley (Hordeum vulgare L.), an experiment was carried out. Factorial experiment was carried out in completely randomized design with three replicates. To create salinity stress, NaCl in osmotic levels at 0 (as control), -4, -8, -12 and -16 bar were used. For seed priming, gibberellin (GA) 50 ppm was used. Our results showed that treatment × drought interaction on these traits: germination percentage, weight of utilized (mobilized) seed, seed reserve utilization efficiency, seedling dry weight and seed reserve depletion percentage were significant. The highest germination percentage, weight of utilized (mobilized) seed, seed reserve utilization efficiency, seedling dry weight and seed reserve depletion percentage were attained from priming by gibberellin at control conditions. Thus, priming increased characteristics as compared to the unprimed. Priming improved seed reserve utilization such as: weight of utilized (mobilized) seed reserve, seed reserve depletion percentage, seed reserve utilization efficiency and seedling growth in barley under salinity stress.


2021 ◽  
Vol 39 (2) ◽  
pp. 62-67
Author(s):  
Sean J. Markovic ◽  
James E. Klett

Abstract Moroccan pincushion (Pterocephalus depressus) is a drought-tolerant perennial that is being used in landscapes throughout arid areas of the western United States. This paper describes two experiments researching vegetative cutting production from stock plants. Moroccan pincushion stock plants received foliar applications of gibberellic acid (GA3), benzyladenine, ethephon, or auxin [indole-3-butyric acid (IBA)] plant growth regulators (PGR). Plant growth regulators were applied singularly and in combination with GA3 to determine efficacy on stock plant growth. A propagation study was conducted simultaneously to determine effects of these different PGR treatments applied to stock plants on the rooting of moroccan pincushion cuttings. The stock plant study showed GA3 + benzyladenine application increased cutting production over other PGR treatments. Fresh weight of moroccan pincushion cuttings did not differ among treatments. While cuttings did not differ in dry weight in experiment 1, statistical differences were observed in experiment 2. However, these differences in dry weight did not affect the quality of the cuttings. Cuttings from stock plants treated with GA3 + IBA treatment had the highest numerical growth index [(height + width + width)/3]. Cuttings from stock plants treated with GA3 alone or in combination with another PGR were all greater in average growth index and statistically differed from those without GA3 being applied. PGR treatments did not affect rooting percentages of the cuttings with nontreated stock plant cuttings successfully rooting at an average rate of 95%. However, GA3 + IBA was the only treatment where cuttings had 100% rooting for both experiments, indicating potential rooting benefits. Index words: Plant growth regulator, propagation, Pterocephalus depressus, vegetative cuttings. Species used in this study: Moroccan pincushion [Pterocephalus depressus Archibald]. Chemicals used in this study: gibberellic acid (GA3), benzyladenine, ethephon, indole-3-butyric acid (IBA).


2018 ◽  
Vol 51 (4) ◽  
pp. 60-72
Author(s):  
E.K. Eifediyi ◽  
F.O. Ogedegbe ◽  
N.B. Izuogu ◽  
C.A. Adedokun ◽  
A. Katibi ◽  
...  

Abstract The Guinea savannah zone of Nigeria is beset by increasing population and infrastructural development, thereby putting pressure on available land with rapidly declining fertility due to low organic matter content, soil erosion, high temperature and seasonal bush burning. Sesame is cultivated in this zone and the yield has remained very low, compared to yield in other parts of the world. This could be attributed to poor nutrient status and poor cultural practices used by peasant farmers. A field experiment was conducted at the Teaching and Research Farm, University of Ilorin, Nigeria, in a southern Guinea savannah zone in 2015 and repeated in 2016 cropping season to determine the effects of 2,4-Dichlorophenoxyacetic acid (2,4-D), a plant growth regulator and NPK fertilizer on the growth and yield of sesame. The experiment was laid out as a factorial arrangement, fitted into a randomized complete block design replicated thrice. The factors imposed were 2,4-D (0, 5 and 10 ppm ha−1) and NPK 15:15:15 (0, 100, 200 and 300 kg ha−1). Data were collected on vegetative traits (plant height, number of leaves, leaf area) and yield components (number of capsules per plant; yield per plant and per hectare). The data were subjected to analysis of variance (ANOVA) using the Genstat statistical package 17th edition and significant means were separated by using the least significant difference at 5% level of probability. The result revealed that using plant growth regulator and NPK fertilizer had significant effects (p<0.05) on plant height (151 cm) and yield per hectare (530 kg/ha). The qualitative and quantitative analysis of the seeds further reaffirmed the presence of bioactive compounds, such as saponins, tannins, flavonoids and phenolic compounds, which are important health promoting food in the seeds.


2021 ◽  
pp. 1-11
Author(s):  
Jonathan Ebba ◽  
Ryan W. Dickson ◽  
Paul R. Fisher ◽  
Crysta N. Harris ◽  
Todd Guerdat ◽  
...  

The overall goal was to evaluate fertilizer options for greenhouse producers, with or without a plant growth regulator (PGR) application, to improve subsequent performance of container-grown annuals. Petunia (Petunia × hybrida) was the model container-grown crop in simulated production and consumer environments. The first experiment at two locations (New Hampshire and Florida) compared strategies using water-soluble fertilizer [WSF (17N–1.8P–14.1K)], controlled-release fertilizers (CRF), and slow-release fertilizers (SRF) that were either applied throughout or at the end of the 8-week production phase [point of shipping (POS)] for petunia rooted cuttings grown in 8-inch azalea containers. In the subsequent simulated “consumer” phase, container plants were irrigated with clear water (no fertilizer) for 6 weeks. Plant performance [number of flowers, SPAD chlorophyll index, dry weight, and tissue nitrogen (N)] at the end of the consumer phase was improved by top-dressing at POS with either CRF or granular organic fertilizer (both at 2.74 g/container N), or preplant incorporation of either a typical CRF at 4.12 g/container N or a CRF with an additional prill coating to delay initial release (DCT) at 2.74 g/container. There was no carry-over benefit from applying a liquid urea-chain product (1.37 or 2.74 g/container N) or top dressing with granular methylene di-urea (2.74 g/container N), or 400 mg·L–1 N (0.2 g/container N) from a liquid organic fertilizer at POS. The consumer benefit of applying 400 mg·L–1 N (0.2 g/container N) from a WSF at POS was increased by supplementing with 235 mg·L–1 magnesium (Mg) and 10 mg·L–1 iron (Fe). A second experiment in 10-inch-diameter pots evaluated the effect on consumer performance from providing 200 or 400 mg·L–1 N of WSF with the PGR paclobutrazol, at the final 1 L/pot irrigation at POS. Application of 3 mg·L–1 paclobutrazol delayed leaf yellowing and reduced plant height, width, and shoot dry weight during the consumer phase, resulting in a more compact growth habit and higher plant quality compared with plants that received no PGR, regardless of WSF treatment. Addition of supplemental 235 mg·L–1 Mg and 10 mg·L–1 Fe to the high rate of WSF and PGR did not improve consumer performance compared with other treatments that included a PGR. Overall, the first experiment demonstrated that the most effective fertilizer strategies require a CRF or SRF that will release nutrients throughout the consumer phase, and that impact of liquid fertilizer options is limited because of lower N supply per container. A single application at POS of a high rate of WSF with supplemental Mg and Fe may have short-term benefits, for example while plants are in a retail environment. Growers should consider combining a residual fertilizer with a PGR application for premium, value-added container annuals.


Author(s):  
Sri Kasmiyati ◽  
Elizabeth Betty Elok Kristiani ◽  
Maria Marina Herawati

Artemisia cina is one of a member of genus Artemisia that has potential as a medicinal plant. However, the levels of Artemisia medicinal bioactive compounds are very low. Polyploidization is an alternative method that can enhance of growth and secondary metabolite productions of plants. The aims of this research were to determine the effect of polyploid induction using colchicine and plant growth regulator toward plant growth, the chlorophyll, kaemferol and quercetin contents of A. cina. Four different A. cina used in this research consisted of two diploid genotypes (TWN and KJT) and two polyploid genotypes (J and M). Induction of mutant polyploid was conducted using colchicine and combination of plant growth regulator benziladenyl (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D). The measured plant growth parameters were dry weight, leaf area, and plant height. The chlorophyll content of leaves was determined spectrophotometrically, and flavonoid content determined using HPLC. The result showed that the polyploid genotype (M) significantly decreased leaf and root growth compared to the KJT and TWN. In A. cina plants, polyploidization using colchicine is more effective in increasing the biomass than using combination plant growth regulator BA and 2,4-D. The flavonoid content of KJT was the lowest, and significantly different compared to the other plants. This study provides new information about the effect of polyploid on growth and flavonoid content in A. cina. This can be  useful information to develop A. cina to become a medicinal plant.


2016 ◽  
Vol 3 (2) ◽  
pp. 90-98
Author(s):  
Pienyani Rosawanti

This research aims to study the growth response of plant leaf cuttings mother in-law�s tongue (Sansevieria parva) origin of cuttings and various plant growth regulator treatment. Research was using complete randomized block design (RAKL) with 6 replications. The first factor was the origin of cuttings, i.e. the top/tip leaves, the middle leave, and the bottom/leaves base. The second factor was the various plant growth regulator (PGR) treatment i.e. onion extracts, water coconut and Rootone-F. Parameters observed and measured is the experiment root length, number of roots, root wet weight and root dry weight. The results showed that plant growth regulator treatment significant effect on root length, number of roots, root wet weight, and root dry weight. The treatment combination of origin of cuttings and synthetic plant growth regulator by cutting material from the middle to give the best results on the number of root and root wet weight.


Author(s):  
Chong Wang ◽  
Fei Gao ◽  
Changjiao Sun ◽  
Yue Shen ◽  
Shenshan Zhan ◽  
...  

1-triacontanol has attracted considerable research interest in plant growth regulator field, owing to its physiological effect in seed germination, root growth and photosynthesis enhancement. In this work, a 1-triacontanol modified...


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