scholarly journals In vitro effect of seed bio-priming techniques on seed germination and seedling vigour of few vegetable crops

2020 ◽  
Vol 12 (4) ◽  
pp. 702-709
Author(s):  
Rakesh Kumar Jaiman ◽  
Sanjay Kumar Acharya ◽  
Naziya P. Pathan ◽  
Amol J. Deshmukh ◽  
Haresh A. Desai ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seed Treatment ◽  
Trichoderma Viride ◽  
Solanum Melongena ◽  
Dry Weight ◽  
Germination Percentage ◽  
Vegetable Crops ◽  
Seedling Vigour ◽  
Vitro Effect

Bio-priming can also be viewed as a new technique of seed treatment using biological agents to stimulate germination of seed and growth of the plant and further protecting the seed from soil-and seed-borne pathogens. The present investigation was carried out in vitro conditions on seed germination and seedling vigour of few vegetable crops viz.  tomato (Solanum lycopersicum L.), brinjal (Solanum melongena L.), onion (Allium cepa L.) and chilli (Capsicum annuum L.), during 2015 and 2016. The treatments comprised viz. T1: Non primed seeds (Control), T2: Seed treatment with Carbendazim 2.5g/kg seed, T3: Hydro-priming for 6 hrs, T4: Hydro-priming for 12 hrs, T5: Hydro-priming for 18 hrs, T6: Biopriming with Trichoderma  viride for 6 hrs, T7: Biopriming with T.viride for 12 hrs, T8: Biopriming with T.viride for 18 hrs, T9: Biopriming with Trichoderma harzianum for 6 hrs, T10: Biopriming with T. harzianum for  12 hrs, T11: Biopriming with T. harzianum for 18 hrs, T12: Biopriming with Pseudomonas fluorescens for 6 hrs, T13: Biopriming with P. fluorescens for 12 hrs and T14: Biopriming with P. fluorescens for 18 hrs.  The results revealed that maxiumum germination percentage (92.92, 90.77,83.00 and 86.33), seedling length (32.38 cm, 29.35 cm, 31.75 and 31.60 cm), seedling fresh weight (2.07 g, 4.01 g, 3.05 g and 2.04 g),  seedling dry weight (0.42 g, 0.86 g, 0.62 g and 0.42 g) and seedling vigour index (3008.11, 2664.00, 2635.00 and 2728.00) were recorded in T10 (bio priming with T. harzianum for 12 hrs) in tomato (S. lycopersicum L.), brinjal (S. melongena L.), onion (A. cepa L.) and chilli (C. annuum L.), respectively. Thus, it indicated that priming of seeds of these crops with T. harzianum/P. fluorescens/ T. viride  for 12 hrs was very effective with respect to their vegetative growth along with the quality yield.

scholarly journals Effect of nano based seed treatment insecticides on seed quality in Pigeonpea

2017 ◽  
Vol 9 (2) ◽  
pp. 1226-1235
Author(s):  
B.N. Raghu ◽  
Basave Gowda ◽  
S. N. Vasudevan ◽  
Sangeeta I. Macha ◽  
Sharan Gouda Hiregoudar ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seed Treatment ◽  
Seed Quality ◽  
Dry Weight ◽  
High Energy ◽  
Seed Kernel ◽  
Neem Seed ◽  
Seedling Vigour ◽  
Vigour Index ◽  
Sweet Flag

A laboratory experiment was conducted to know the effect seed treatment with nano insecticides on seed quality of pigeonpea (Cajanus cajan (L.) Millsp.) cv. TS3R. This study was conducted to evaluate the effect of macro and nano insecticides on seed germination and vigour of Pigeonpea. Different recommended seed treatment insecticides viz, malathion, fenvalerate, emamectine benzoate, thiodicarb, sweet flag and neem seed kernel powder insecticides were synthesized to nano form using high energy planetary ball mill. The Pigeonpea seed were treated with different nano insecticides i.e., 10-90 per cent reduction in actual dosage. Among the different treatments studied, seed treated with nano malathion 50 per cent lesser than normal dosage, fenvalerate 60 per cent lesser, thiodicarb 10 per cent lesser, emamectine benzoate 30 per cent lesser, sweetflag 70 per cent lesser, neem seed kernel powder 40 per cent lesser than actual recommended dosage gave significantly higher seed germination (98.0, 98.67, 98.67, 97.0, 99.0 and 98.67 percent) ,less number of abnormal seedlings (1.0, 0.33, 1.0, 1.0, 1.0 and 0.33 per cent) , shoot length (10.13, 9.00, 11.47, 9.50, 10.90 and 10.87 cm), root length (12.56, 12.93, 12.83, 12.60 11.50 and 13.00 cm), seedling dry weight (85.73, 87.40, 88.47, 87.70, 88.60 and 88.27 g) and seedling vigour index (2223, 2164, 2397, 2143, 2217 and 2354) as compared to untreated seeds and macro insecticides. Therefore, it is very clear that nano based insecticides has a significant (0.1 %) impact on the seed quality improvement.

In Vitro Investigation of Biopriming on Seed Quality Parameters in Green Gram [Vigna radiata (L.)]

2019 ◽  
Author(s):  
Ashok S Sajjan ◽  
Sujaya Waddinakatti ◽  
R B Jolli ◽  
Geetha D Goudar
Keyword(s):  
Rhizobium Leguminosarum ◽  
Seed Quality ◽  
Dry Weight ◽  
Germination Percentage ◽  
Quality Parameters ◽  
Fluorescent Pseudomonad ◽  
Seedling Vigour ◽  
In Vitro Investigation ◽  
Vigour Index

The in vitro investigation was conducted during the kharif 2017 in the Department of Seed Science and Technology, College of Agriculture, Vijayapura. The experiment comprised of nine biopriming treatments along with control. The experiment was laid out in CRD with three replications. The seeds were subjected to in vitro studies and data was analysed statistically. The seed bioprimed with Rhizobium leguminosarum @ 20 per cent recorded higher seed germination percentage (90.50 %), shoot length (19.27 cm), root length (12.38 cm), seedling length (31.65 cm), seedling dry weight (373.0 mg), seedling vigour index I (2864), seedling vigour index II (33.75), dehydrogenase enzyme activity (1.010 OD value) and lower electrical conductivity (0.107 dS m-1) followed by Pseudomonas fluorescens @ 20 per cent, Fluorescent pseudomonad sp. @ 20 per cent as compared to control.

scholarly journals SEED GERMINATION BEHAVIOUR, SEEDLING GROWTH, MORPHO-PHYSIOLOGICAL AND YIELD ATTRIBUTES OF RICE GROWN IN CADMIUM-ADDED SOIL

2021 ◽  
Vol 56 ◽  
pp. 113-127
Author(s):  
M. Zahidul Islam Hawlader TUHIN ◽  
◽  
Taufika Islam ANEE ◽  
Naznin AHMED ◽  
Mira RAHMAN ◽  
...  
Keyword(s):  
Seed Germination ◽  
Block Design ◽  
Dry Weight ◽  
Cadmium Stress ◽  
Germination Percentage ◽  
Dependent Manner ◽  
Cd Stress ◽  
Seedling Vigour ◽  
Yield Attributes ◽  
Spad Value

An experiment was conducted to investigate the effect of different levels of cadmium (Cd) stress on germination, morphology, physiology and yield of rice. There were five treatments, viz. control (no Cd), 0.5 mM CdCl2, 1 mM CdCl2, 1.5 mM CdCl2 and 2 mM CdCl2, carried out by a randomized complete block design (RCBD) with three replications. Cadmium stress caused a significant reduction of plant height and tillers hill-1 in a dose-dependent manner. The highest decline of relative water content and SPAD value was found at 1.5 mM (11 and 16%) and 2 mM (14 and 19%) Cd stress compared to the control. The number of effective tillers hill-1, filled grains panicle-1, 1000-grain weight, length of panicle, grain yield and straw yield reduced significantly by 44, 32, 15, 24, 37 and 45%, respectively, over the control. Germination and seedling-related parameters were recorded 10 days after germination. The lowest germination percentage (27%), germination index (27%) and seedling vigour index (49%) were observed at the highest level of Cd stress in comparison to the control. Cadmium stress resulted in maximum abnormal seedlings and markedly reduced length of root and shoot as well as their fresh and dry weight. Root shoot ratio showed differential responses to Cd stress. Thus, it can be concluded that a higher level of Cd in soil has a detrimental effect on seed germination, morphology, physiology and yield of rice.

Effects of Na2CO3 on seed germination, seed reserve utilizationand seedling growth in bitter vetch (Vicia ervilia L.)

2016 ◽  
Vol 39 (4) ◽  
Author(s):  
Ali Sepehri ◽  
Maryam Saman ◽  
Somayeh Bayat
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Dry Weight ◽  
Germination Percentage ◽  
Shoot Length ◽  
Utilization Efficiency ◽  
Limiting Factors ◽  
Alkaline Soils ◽  
Seedling Vigour ◽  
Bitter Vetch

Alkalinity is one of the main limiting factors of seed germination in alkaline soils. The aim of this study was to evaluate the effects of Na2CO3 levels (0, 5, 10, 15, 20 and 25 mM) on seed germination, seed reserve utilization and seedling vigour of three local bitter vetch cultivars (Buinzahra, Shahreza and Tarom). The results indicated that increasing Na2CO3 concentrations caused a decrease in germination percentage (GP), germination rate (GR), seedling length, seedling dry weight (SLDW) and seed reserve utilization efficiency (SRUE). The rate of reduction in shoot length in comparison with the control in different concentrations of Na2CO3 was detected from Buinzahra as 30-92%, Shahreza 39-97% and Tarom 13-83%. Decrease in root length was more than shoot length in all the cultivars at each Na2CO3 level, as compared to the control. The weight of mobilized seed reserve (WMSR) and seed reserve depletion percentage (SRDP) decreased with increasing Na2CO3 levels up to 10 mM in Buinzahra and Tarom cultivars and up to 15 mM in Shahreza. WMSR and SRDP then rapidly raised with higher alkalinity levels. These results suggest that reduction of seed germination and decline in seedling dry weight in response to alkalinity stress is a consequence of decline in seed reserve utilization efficiency.

scholarly journals In vitro Effect of Caesalpinia sappan and Crateva magna Extracts in Enhancing Seed Germination and Seedling Growth of KDML105 Rice Variety

2021 ◽  
Author(s):  
Arom Jantasorn ◽  
Ponpawit Pongsupap ◽  
Thanaprasong Oiuphisittraiwat
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Seed Treatment ◽  
Growth Parameters ◽  
Dry Weight ◽  
Rice Seedling ◽  
Rice Seed ◽  
Caesalpinia Sappan ◽  
Vigor Index ◽  
Vitro Effect

Background: Rice (Oryza sativa L.) is a major cereal crops planted in Thailand for both local consumption and export. The objective of this study was to investigate the allelopathic effect of Caesalpinia sappan L. and Crateva magna (Lour.) DC. extracts in promoting a rice cultivar, Khao Dowk Mali 105 (KDML105) seed germination and seedling growth, at 7 and 14 days after sowing (DAS). Methods: The C. sappan and C. magna plants were cleaned with tap water and air dried in room temperature then cut into small pieces and ground into fine powder. Plant crude extracts was prepared using ethanol as solvent. Result: The results showed that rice seed treatment with C. sappan and C. magna extracts was significant (all p less than 0.05), increasing root length, shoot fresh and dry weight and root fresh and dry weight of rice seedlings at 14 DAS. Seed treatment with these two plant extracts led to a significantly greater vigor index of the KDML105 rice seedling cultivar, when compared with the control. Interestingly, the seed treatment with C. magna extract at a 100 ppm concentration was found to produce the highest enhancement in the overall rice seedling growth parameters, including the vigor index of seedlings. The results showed that a low concentration of C. magna extracts can be potentially used in promoting seedling growth of rice since they improved the overall growth parameters. 

scholarly journals Effect of harvest time and fruit size on seed germination and seedlings growth of sour orange and Mexican lime under in vitro conditions

2021 ◽  
Vol 35 (2) ◽  
Author(s):  
Sakineh Jokari ◽  
Akhtar Shekafandeh
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Fruit Size ◽  
Dry Weight ◽  
Germination Percentage ◽  
Sour Orange ◽  
Mexican Lime ◽  
Randomized Design ◽  
Fruit Harvest

The aim of this research was to determine the best time to harvest the fruits for seed production which would ultimately lead to the production of citrus rootstocks of optimum quality. The sour orange and Mexican lime fruits were harvested on 7 and 5 occasions, respectively. The very first fruits were harvested 80 days after flowering and subsequent harvests were gathered every 30 days. An in vitro experiment was carried out in a completely randomized design, with four replications and 20 seeds in each replication. Based on fruit growth curve the time of fruit harvest affected seed germination (percentage and rate) and seedling growth (stem and root length, fresh and dry weight of stems, roots and leaves).  The results showed that the best time to harvest the fruits of sour orange and Mexican lime was 230 and 170 days after flowering, respectively, which led to maximum seed germination (Mexican lime 100% and sour orange 85%) and seedling growth. The highest root, stem and leaf fresh and dry weight was also obtained at 230 and 170 days after flowering in sour orange and Mexican lime respectively.   

scholarly journals Performance of Bioprimed Chilli Seed under Moisture Stress Condition

2019 ◽  
pp. 1-7
Author(s):  
M. Ananthi ◽  
P. Selvaraju ◽  
K. Sundaralingam
Keyword(s):  
Seed Germination ◽  
Pseudomonas Fluorescens ◽  
Stress Condition ◽  
Trichoderma Viride ◽  
Moisture Stress ◽  
Germination Percentage ◽  
Seedling Vigour ◽  
Control Seed ◽  
Vigour Index ◽  
Improve Seed Germination

Laboratory experiment was carried out using ‘PKM 1’ chilli (Capsicum annuum L.) seed to standardize bioprimed seeds under moisture stress condition inorder to improve seed germination and seedling vigour. To induce the moisture stress, optimize the different concentration of water holdings capacity viz., 80, 60, 40 and 20% were used for best bioprimed seed treatment (biocontrol agents (Pseudomonas fluorescens 60% 12 h, Trichoderma viride 60% 9 h) and liquid biofertilizers (Azospirillum 10% 9 h and Phosphobacteria 15% 9h )) along with hydroprimed seed and control seed. Seed bioprimed with Pseudomonas fluorescens 60% for 12 h improved the germination percentage ( 82), root length (14.2 cm), shoot length (6.2 cm), dry matter production (0.0489 g 10 seedlings-1) and vigour index (1673) compared to control seed. Seed biopriming with  Pseudomonas fluorescens 60% for 12 h can be adopted to improve seed germination and seedling vigour under moisture stress condition upto 20%.

scholarly journals Swailing affects seed germination of plants of European bio-and agricenosis in a different way

2017 ◽  
Vol 12 (1) ◽  
pp. 62-75 ◽  
Author(s):  
Renata Bączek-Kwinta
Keyword(s):  
Seed Germination ◽  
Agricultural Practice ◽  
Seedling Vigour ◽  
Habitat Composition ◽  
Indirect Impact ◽  
Wild Thyme ◽  
Plant Habitat ◽  
Seed Testa ◽  
Stimulation Of

AbstractSwailing as a part of agricultural practice is an illegal habit in many European countries. The indirect effect of swailing is the emission of volatiles (SGV), hence the aim of the study was to identify their impact to seeds of different species occurring or grown Europe. It was carried out on seeds of 29 species of 10 botanical families within the angiosperms. The response to SGV was more or less differentiated within a family, and even within the species, e.g. in the case of tomato. The stimulation of germination and/or increased seedling vigour was established in celery, green- and red-leafed basil, white and red cabbage, white clover and wild thyme. The same effect was noticed for the seeds of stratified broadleaf plantain and the positively photoblastic seeds of German chamomile germinated in darkness. The inhibition of seed germination and/ or reduced seedling vigour was demonstrated in case of caraway, dill and forget-me-not. Similar results were obtained in the experiments carried outin vitroand in the soil, hence it can be assumed that the indirect impact of SGV on plant habitat composition is likely. The interaction of SGV compounds with seed testa and seed phytohormones is discussed.

scholarly journals IDENTIFICATION OF DROUGHT TOLERANT WHEAT GENOTYPES UNDER WATER DEFICIT CONDITIONS

2016 ◽  
Vol 4 (2) ◽  
pp. 206-214 ◽  
Author(s):  
Zaid Chachar ◽  
N. A. Chachar ◽  
Q.I. Chachar ◽  
S.M Mujtaba ◽  
G.A Chachar ◽  
...  
Keyword(s):  
Water Stress ◽  
Seed Germination ◽  
Osmotic Stress ◽  
Root Length ◽  
Dry Weight ◽  
Shoot Length ◽  
Wheat Genotypes ◽  
Maximum Reduction ◽  
Drought Tolerant

Climate change is emerging phenomena and causing frequent drought which lead to scaricity of water, which ultimately nagetively affecting wheat (Triticumaestivum L.) yield all around the world. The aim of this study was to explore the potential deought tolerant wheat genotypes for candidate genes exploration. This study was conducted during the year 2014-2015 at Plant Physiology Division, Nuclear Institute of Agriculture (NIA) Tandojam. The six wheat genotypes (cv. MT-1/13, MT-2/13, MT-3/13, MT-4/13 Chakwal-86 and Khirman) were investigated for their response at germination and seedling stage under different water stress treatments (0, -0.5, -0.75 and -1.0 MPa) in controlled conditions. The results of experiments with reference to genotypes revealed that genotype Chakwal-86 shows maximum seed germination (82.58 %), while the genotype Khirman shows maximum shoot length  (7.23 cm), root length  (15.1 cm), shoot fresh wt. (5.85 g 10-1shoots), root fresh wt.  (3.45 g 10-1roots), shoot dry wt. (1.33 g 10-1shoots), root dry wt. (0.69 g 10-1roots). Among the genotypes tested Khirman and MT-4/13 are the tolerant genotypes had the potential to perform better under drought conditions, whereas  MT-4/13 and Chakwal-86 were moderate tolerant under water stress conditions. Moreover, the genotypes i.e. MT-1/13 and MT-2/13 are the sensitive genotypes under drought environment. It is concluded from present in-vitro studies that osmotic stress significantly reduced the seed germination shoot/root length fresh and dry weight in all six wheat genotypes. The maximum reduction was found at higher osmotic stress induced by PEG-6000 (-1.0 MPa) significantly.

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