The effect of chemical mutagens on the characteristics of plants Nigella damascena L. variety Bereginya in M1 generation

We studied the effect of chemical mutagens EMS, NMU and a new mutagen DG-2 on the traits of Nigella damascena L. variety Bereginya in the generation M1. It was found that the treatment of love-in-a-mist seeds with these substances changed such characteristics as plant survival, plant height, and the number of seeds per plant. The greatest influence on these indicators was exerted by nitrosomethylurea. In our study, all chemical mutagens led to a decrease in plant survival and mainly caused plant oppression. In all treatments, a decrease in the total plant height was revealed in comparison with the control. In some cases the number of seeds per plant has decreased. The tested mutagens caused the appearance of morphoses in plants with a frequency of 3.87% to 0.48%, characterized by a change in color and shape of the leaves and the plant dwarfism. The appearance of plants with altered characteristics in the M1 generation assumes hereditary mutations of a different nature in the subsequent generations.

The effect of the chemical mutagens EMS (ethyl methanesulfonate), NMU (nitrosomethylurea), and the new mutagen DG-2 on a number of traits of Nigella damascena L. of the variety “Charivnytsya” in the M1 generation was studied. It was found that treating love-in-a-mist with these chemical mutagens changed such characteristics as plant survival, plant height, number of capsules per plant, number of side shoots and number of seeds from one plant. The dependence of the change in the studied traits on the type of chemical mutagen, its concentration and exposure duration was revealed. Nitrosomethylurea had the greatest influence on plant survival. After treatment with this mutagen at a maximum concentration of 0.05% and with a maximum exposure of 16 hours, the number of surviving plants was 4.3%. The effect of DG-2 mutagen at the concentration of 0.5% and 16 hours’ exposure on the survival of M1 plants was the least different from the control group. In this treatment 60.6% of plants survived, with a control value of 61.7%. The tested mutagens caused the appearance in plants of chlorophyll morphoses with a frequency of up to 0.93%, they were characterized by a change in the color of the leaves and capsule leaflets. Nitrosomethylurea had the greatest influence on the plant height and the number of seeds per plant at the concentrations of 0.01 and 0.05% with 6 and 16 hours’ exposure, respectively. After treatment with NMU at the concentration of 0.05% with exposure of 16 hours all plants were found to be unviable. After treatment with NMU at the concentration of 0.01% with 6 hours’ exposure, the average number of seeds per plant was minimal - 17.5 pieces, and plant height - 25.9 cm. The smallest but statistically significant effect on the studied parameters was noted after treatment with mutagen DG-2. In the case of the mutagen concentration of 0.05% and 16 hours’ exposure the average plant height was 40.7 cm, and the average number of seeds - 112.0 pieces, with control values of 47.0 cm and 237.1 pieces, respectively. The revealed changes in the M1 generation imply mutations of different directions in the next generation of love-in-a-mist.


2017 ◽  
Vol 23 (1) ◽  
Author(s):  
A. B. SAGADE

The study of the effect of three well known mutagens, ethyl methane sulphonate (EMS), methyl methane sulphonate (MMS) and gamma rays (GR) on the yield contributing traits of the urdbean variety TPU-4 were carried out in the M3 generation. Effect of selected mutagenic treatments/doses of EMS (0.02, 0.03 and 0.04 M), MMS (0.0025, 0.05 and 0.01 M) and (GR) (30, 40 and 50 KR) on different yield contributing traits like plant height, plant spread, number of pods per plant, pod length, number of seeds per pod, seed yield per plant and 100 seed weight were analyzed in the M3 populations of the variety TPU-4. Seeds of M2 plants and control were harvested separately and sown to raise M3 population.. Genetic variabilty in the mutagen administered M3 progeny of the urdbean variety TPU-4 was analyzed by employing statistical methods. Data on mean values and shift in the mean of seven quantitative traits was evaluated on individual plant basis. The experimental findings revealed that concentrations / dose of the all these mutagens showed inhibitory effect on plant height, number of pods per plant, pod length and number of seeds per pod. Lower concentrations of mutagens exerted a promotory effect on plant spread, 100 seed weight and seed yield per plant while higher concentrations of these mutagens inhibited them to different extent.


2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Raphael Adu-Gyamfi ◽  
Ruth Prempeh ◽  
Issahaku Zakaria

In Ghana, sesame is cultivated in some districts of northern Ghana. Genotypes cultivated are land races that are low yielding leading to decline in production. There is the need for improvement of these land races to generate high yielding cultivars. Characterization of genetic diversity of the sesame land races will be of great value in assisting in parental lines selection for sesame breeding programmes in Ghana. Twenty-five sesame land races were collected from five districts in northern Ghana noted for sesame cultivation. Seeds collected were planted in three replicates in randomized complete block design and were evaluated for a number of morphological characters. Data collected were subjected to Principal Component Analysis (PCA) and a dendrogram showing similarity between the accessions were drawn. Data on number of capsules per plant, number of seeds per capsule, and plant height at flowering were subjected to analysis of variance using GenStat Discovery Edition 4. Molecular genetic diversity was assessed by using thirty eight SSR markers widely distributed across sesame genome to characterize the materials. Twenty-one out of the 38 primers were polymorphic. Cluster analyses using the Euclidean similarity test and a complete link clustering method were used to make a dendrogram out of the morphological data. Analysis of variance showed that capsule number was significantly different; a range of 54.9 and 146.7 was produced. The number of seeds per capsule varied significantly and the variation between highest and lowest accession in seed production was 33%. Plant height was also significantly different ranging from 60.6 to 94.1 cm. Using morphological traits the accessions clustered into two major groups and two minor groups and variation among accessions were 10-61%. On the other hand, SSR marker-based dendrogram revealed five major and two minor groups. It showed that variation among the accessions was low, 10-20%. Heterozygosity was 0.52, total alleles produced were 410, and average allele per locus was 19.52. Six accessions, C3, C4, S5, W1, W3, and W5 fell in five different clusters in the SSR dendrogram and in six clusters in the morphomolecular based dendrogram. These accessions were noted for high capsule number per plant and seeds number per capsule and are recommended for consideration as potential parental lines for breeding programme for high yield.


2019 ◽  
Vol 17 (1) ◽  
pp. 33-38
Author(s):  
Swapan Kumar Paul ◽  
Mosa Morsheda Khatun ◽  
Md Abdur Rahman Sarkar

Sulphur is a component of plant amino acids, proteins, vitamins, and enzyme structures which influence the productivity of oil seed and total oil content. The experiment was conducted to find out the effect of sulphur on the seed yield and oil content of sesame in Bangladesh. The experiment comprised three varieties of sesame viz. Binatil-2, Binatil-3 and BARI Til-4 and six levels of sulphur (S) viz. 0, 10, 20, 30, 40 and 50 kg S ha–1. The experiment was laid out in a randomized complete block design with three replications. Dry matter production, crop characters, yield components, seed yield and oil content were significantly influenced by variety, level of sulphur and their interaction. The highest dry matter production plant–1 at 50 DAS (17.56 g), plant height (101.3 cm), number of branches plant–1 (3.66),  number of pods plant-1 (41.56), number of seeds pod-1 (58.83),  seed yield    (747.2 kg ha-1), stover yield (2243.0 kg ha–1) and oil content (40.03%) were obtained in BARI Til-4 while the corresponding lowest values of all parameters were recorded in Binatil-2. In case of sulphur application, the highest dry matter production plant–1 at 50 DAS (20.81 g), plant height (109.7 cm), number of branches plant–1 (3.87),  number of pods plant–1 (46.13),  number of seeds pod-1 (56.67),  seed yield (800.0 kg ha–1), stover yield (2787 kg ha–1 ) and oil content (43.97%) were obtained when crop was fertilized with 30 kg S ha–1 while the lowest seed yield (502.2 kg ha–1), stover yield (1550.0 kg ha–1) and oil content (32.80%) were obtained in control (0 kg S ha–1). BARI Til-4 fertilized with 30 kg S ha–1 produced the highest dry matter plant–1 at 50 DAS (24.80 g), number of pods plant–1 (51.13), seeds pod–1 (62.0) and seed yield (1011.0 kg ha–1). The highest oil content (43.97%) was also recorded in BARI Til-4 fertilized with 30 kg S ha–1, which was as good as that of BARI Til-4 fertilized with 40 kg S ha–1. Therefore, BARI Til-4 fertilized with 30 kg S ha–1 can be considered as a promising practice in respect of seed yield and oil content of sesame in Bangladesh. J. Bangladesh Agril. Univ. 17(1): 33–38, March 2019


2017 ◽  
Vol 16 (2) ◽  
Author(s):  
Ridwan Kusuma ◽  
Nyimas Sa’diyah ◽  
Yayuk Nurmiaty

Soybean consumption here in Indonesia continues to increase each year that is notaccompanied by an increase in soybean production. One way to increase soybeanproduction by using improved varieties. The purpose of this study were (1) Estimating thevalue of the diversity of phenotypes soy F6 generations from crosses between Wilis x Mlg2521,(2) Estimating the heritability estimates soybean F6 generations from crosses Wilis x Mlg2521,(3) Know the numbers expectation of F6 generation crosses Wilis x Mlg2521. The researchwas conducted from March 2014 until June 2014 at the Land Lab Lampung StatePolytechnic and observations made in Laboratory of Seed and Plant Breeding, University ofLampung. Soybean seeds used were F6 generation zuriat from Wilis x Mlg2521, Wilis andMlg2521. The treatment laid out in a randomized block design, 2 replications. The resultsshowed that (1) The diversity of phenotypes population F6 for characters date of flowering,harvesting age, plant height, number of productive branches, total number of pods plant,weight of 100 grains, grain weight plant and number of seeds plant including all the broadcriteria, (2) The value of heritability estimates the population F6 for characters date offlowering, harvesting age, plant height, number of productive branches, total number ofpods plant, weight of 100 grains, grain weight per plant and number of seeds plantbelonging to the high criteria, (3) Number expectations of the population F6 is 7-64-1-8 and7-64-1-3.Keywords: heritability, phenotypic variance, soybean


2020 ◽  
Vol 8 (03) ◽  
pp. 289-295
Author(s):  
Warlinson Girsang ◽  
Rosmaria Girsang

The research was carried out in Dolog Masagal Subdistrict, Simalungun Regency, altitude ± 1.100 m above sea level. The research was conducted from January to May 2019. The purpose of this study was to determine the tolerance level of several hybrid corn varieties to cob rot disease. Methods for conducting the research used randomized block design (RBD) with 1 factor. Types of hybrid corn varieties tested were: Pioneer 29 (V1), NK 99 (V2), Pioneer 12 (V3), NK 22 (V4), Pioneer 4 (V5), Nusantara I (V6), Asia 1 (V7).The parameters observed were: plant height, cob diameter, cob length containing seeds, number of seed rows per cob, number of seeds per row, number of seeds per cob,  husk cover in cob, cob rot intensity, the amount of dry production per plot, and the weight of 1.000 seeds. Based on the research results, different types of hybrid corn varieties produce different tolerances of cob rot resistance in the Simalungun highlands. The most tolerant variety of cob rot disease is Pioneer 29 (mild damage scale = 0 - 10%). Different types of varieties planted also affect various components of growth and production, among others: plant height, number of seeds per row, number of seed rows per cob, husk cover on cob, dry production per plot, and weight of 100 grains. As for the parameters of cob diameter, cob length containing seeds and the number of seed rows per cob, the difference in varieties did not significantly affect.


Agriculture ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 412 ◽  
Author(s):  
Mariusz Jerzy Stolarski ◽  
Michał Krzyżaniak ◽  
Dariusz Załuski ◽  
Józef Tworkowski ◽  
Stefan Szczukowski

Perennial crops harvested in short rotations provide substantial amounts of biomass. This study determined the survival rate, biometric features and yield of fresh and dry biomass of 15 willow genotypes (including seven varieties and eight clones), cultivated at two different sites in two consecutive three-year harvest rotations. The study revealed the very high impact of the genotype (81% of the total variance) on the willow yield. The harvest rotation, along with the genotype, had a significant impact on the plant survival rate and the number of shoots per stool. Willow biomass was mainly affected by the plant height, its survival rate and shoot diameter. The significantly highest fresh (106 Mg ha−1) and dry biomass yield (54.0 Mg ha−1) was obtained from the Żubr variety of S. viminalis, which distinguished this variety from the other genotypes. The mean yield for the best three and five genotypes was 13% and 17% lower, respectively, and the mean yield for the whole experiment was 37% lower compared to the mean yield of the best variety (Żubr). Therefore, the choice of a willow genotype is of key importance for successful willow production.


2018 ◽  
Vol 10 (9) ◽  
pp. 6 ◽  
Author(s):  
Andre A. Diatta ◽  
Wade E. Thomason ◽  
Ozzie Abaye ◽  
Larry J. Vaughan ◽  
Thomas L. Thompson ◽  
...  

Mungbean [Vigna radiata (L.) Wilczek] is a short-duration and relatively drought-tolerant crop grown predominantly in the tropics. This grain legume can improve soil fertility through biological nitrogen (N) fixation. To assess the effects of Bradyrhizobium (group I) inoculation on yield and yield attributes of mungbean, a greenhouse study was conducted during Fall 2016 with two mungbean cultivars (‘Berken’ and ‘OK2000’), two inoculum treatments (inoculated and uninoculated), and two soil textures (loamy sand and silt loam). Pots were laid out in a completely randomized design and treatment combinations were replicated seven times. The main effects of cultivar and soil texture significantly (P ≤ 0.05) affected mungbean seed weight and plant residue mass. Seed yield (13%), plant residue (22%), and protein content (6%) of OK2000 were significantly higher than Berken cultivar. A 31% seed yield and 40% plant residue increase were recorded on silt loam soil compared to loamy sand soil. Significant increase in plant height (18%) and number of pods per plant (21%) were also recorded when mungbean plants were grown on silt loam compared to loamy sand soil. Bradyrhizobium inoculation significantly increased the number of pods per plant, the number of seeds per plant, and seed yield. [Cultivar × inoculation] and [cultivar × soil texture] interactions had significant (P ≤ 0.05) effects on number of seeds per pods and plant height, respectively. Understanding the agronomic practices and soil physical properties that may limit mungbean production could help in optimizing its establishment and growth in non-traditional growing areas.


Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 725 ◽  
Author(s):  
Lisa Burgel ◽  
Jens Hartung ◽  
Daniele Schibano ◽  
Simone Graeff-Hönninger

The impact of exogenously applied plant growth regulators (PGR), 1-naphthalenaecetic acid (NAA), 6-benzylaminopurine (BAP), and a mixture of both (NAA/BAP-mix), was investigated in regard to plant height, length of axillary branches, number of internodes, biomass yield and cannabinoid content of three different phytocannabinoid-rich (PCR) Cannabis genotypes. The results showed that total plant height was significantly reduced under the application of NAA (28%), BAP (18%), and NAA/BAP-mix treated plants (15%). Axillary branch length was also significantly reduced by 58% (NAA) and 30% (NAA/BAP-mix). BAP did not significantly reduce the length of axillary branches. The number of internodes was reduced by NAA (19%), BAP (10%), and the NAA/BAP-mix (14%) compared to the untreated control. NAA application influenced the plant architecture of the tested cv. KANADA beneficially, resulting in a more compact growth habitus, while inflorescence yield (23.51 g plant−1) remained similar compared to the control (24.31 g plant−1). Inflorescence yield of v. 0.2x and cv. FED was reduced due to PGR application while cannabinoid content remained stable. Overall, the application of PGR could be used on a genotype-specific level to beneficially influence plant architecture and optimize inflorescence yield per unit area and thus cannabinoid yield, especially in the presence of space limitations under indoor cultivation.


2018 ◽  
Vol 6 (2) ◽  
pp. 214
Author(s):  
Md. Isfatuzzaman Bhuyan ◽  
K M. Mehadi Hassan ◽  
Nowrose Jahan Lipi ◽  
Md Rafiq Uddin ◽  
Md Monirul Islam ◽  
...  

A study was conducted in the Department of Agronomy, Bangladesh Agricultural University (BAU), Mymensingh from April to August 2012 to examine the salinity tolerance of eight jute varieties (CVE-3, C-83, CVL-1, BJC-7370, O-795, O-9897, OM-1, O-72) and two kenaf varieties (HC-95 and HC-2). Initially germination of these varieties were evaluated under six salinity levels viz. 0mM, 20mM, 40mM, 60mM, 80mM, and 100mM NaCl in the seed laboratory of the Department of Agronomy. Afterwards, all the varieties was grown in pots in the net house under four salinity levels viz. 0mM, 25mM, 50mM, and 75mM. The results from the germination study revealed that under control condition (0mM NaCl) all the jute varieties showed germination more than 80% both at 7 and at 14 days after seed sowing, whereas kenaf varieties had germination a little less than 80%. Among the jute varieties, O-72 showed the highest germination (92%), which was statistically similar with those of OM-1(91%), O-795 (90%), and C-83(87%). Salinity stress decreased germination drastically in all of the jute and kenaf varieties. A salinity level of 100mM caused the highest germination inhibition (74.70%) in jute variety CVL-1, which was very close to those of BJC-7370 and O-72. On the other hand, the lowest germination inhibition (51.11%) was recorded in jute variety O-795. The results of the pot trail showed that the plant characters of jute and kenaf varieties were affected significantly by salinity stress. All the varieties produced their respective plant height, number of leaves per plant, and plant dry weight under control condition (no salinity). Among these varieties, CVE-3 produced the highest plant height (145.2cm), and total dry weight (22.55g), whereas O-72 produced the highest number of leaves per plant (24.67). All these plant characters decreased sharply due to salinity stress irrespective of variety. However, the rate of decrease of plant characters occurred differentially in the jute and kenaf varieties. The highest rate of decrease in number of leaves (74.22%) was found from the variety CVE-3, whereas the lowest one was recorded from the variety HC-2 (51.68%). Salinity stress caused the highest decrease in total plant dry weight (73.68%) in the variety CVL-1 and plant height (73.64%) also in the variety CVL-1. On the contrary, the lowest decrease in plant dry weight (50.99%) was found in the variety O-9897 and plant height also in variety O-9897 (50.88%). Based on the results from germination test and pot trail, it can be inferred that jute variety O-9897 appeared to be the most salt tolerant followed by O-795, HC-2, HC-95, CVE-3, O-72, C-83, BJC-7370, OM-1, and CVL-1.    


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