scholarly journals ULTRAMICROSCOPIC ERYTHROCYTES PROFILE AS A COMPONENT OF THE BABESIOSIS PATHOGENESIS

2021 ◽  
Vol 74 (4) ◽  
pp. 911-914
Author(s):  
Inna I. Torianyk
Keyword(s):  
Energy Dispersive Spectrometer ◽  
Three Dimensional ◽  
Healthy People ◽  
Domestic Dogs ◽  
Scanning Electronic Microscopy ◽  
Electronic Microscopy ◽  
Size And Shape ◽  
Comparison Results ◽  
Three Dimensional Coordinate ◽  
Scanning Electron

The aim is to identify the ultramicroscopic features of the erythrocytes as a component of the babesiosis pathogenesis using scanning electronic microscopy. Materials and methods: Blood samples from 18 domestic dogs with clinically, microscopically, molecularly and genetically confirmed babesiosis served as the study material. The group of comparative control consisted of clinically healthy people (n=31) and domestic dogs (n=6). The method of scanning electron microscopy was used to study the spatial (three-dimensional coordinate system: the plane formed by the axes OX; OY; OZ) pathology of the size and shape of erythrocytes, microrelief of blood cells surfaces, membranopathies (raptures, macromolecules, conglomerates, micropores). The native peripheral blood smears of various origins were performed in accordance with the generally accepted method without fixation, staining/contrast. For this purpose, 0.01 ml of native blood was applied to the degreased surface of the metal platform (S=1 cm2), dried in the air of the laboratory premise, analyzed (× 2000), using the resources of the equipment (SEO-SEM Inspect S50-B; energy dispersive spectrometer AZtecOne with detector X-MaxN20 (Oxford Instruments plc, UC) and power source in the gun: tungsten cathode with thermoelectron emission. This scanning electron microscope made it possible to examine the object under conditions of accelerating voltage of 100 kV. The analysis of the obtained results was performed in comparison. Results: Under conditions of babesiosis development, the erythrocyte link of hematopoiesis responded by the reduction of the number of normocytes (99.0 % in clinically healthy people and domestic dogs, 77.3 % in dogs with babesiosis), increased of the number of regenerative forms of erythrocytes (1% in clinically healthy people and domestic dogs, 1,5 % in dogs with babesiosis), appearanced of degenerative forms of erythrocytes with characteristic pathology of cell size and shape, membranopathies (damage of the integrity of the membrane with the formation of defects). Conclusions: Changes of the ultramicroscopic erythrocytes profile is one of the leading component of the babesiosis pathogenesis. The use of scanning electronic microscopy helps to obtain ultramicroscopic data on the presence of extraerythrocytic forms of Babesia on the background of the impossibility of diagnosis of anisochromia with detailing of inclusions in erythrocytes.

scholarly journals The Use of Calcium Lactate to Enhance the Durability and Engineering Properties of Bioconcrete

2021 ◽  
Vol 13 (16) ◽  
pp. 9269
Author(s):  
Saddam Hussein Abo Sabah ◽  
Luis Hii Anneza ◽  
Mohd Irwan Juki ◽  
Hisham Alabduljabbar ◽  
Norzila Othman ◽  
...  
Keyword(s):  
Calcium Lactate ◽  
Engineering Properties ◽  
Optimal Conditions ◽  
Scanning Electronic Microscopy ◽  
Electronic Microscopy ◽  
Sem Images ◽  
X Ray ◽  
Predicted Values ◽  
Potential Option ◽  
Scanning Electron

This study investigated the optimization of the bioconcrete engineering properties and durability as a response of the calcium lactate (CL) content (0.22–2.18 g/L) and curing duration (7–28 days) using the response surface methodology (RSM). Scanning electronic microscopy (SEM) was conducted to evaluate the microstructure of calcium precipitated inside the bioconcrete. The results indicated that the optimal conditions for the engineering properties of concrete and durability were determined at 2.18 g/L of CL content after 23.4 days. The actual and predicted values of the compressive strength, splitting tensile strength, flexural strength, and water absorption were 43.51 vs. 43.43, 3.19 vs. 3.19, 6.93 vs. 5.50, and 7.55 vs. 7.55, respectively, with a level of confidence exceeding 95%. The scanning electron microscope (SEM) images and energy-dispersive X-ray spectroscopy (EDX) proved that the amount of calcium increased with the increase in CL content up to 2.81 g/L at 23.4 days, reducing the pores inside the concrete and making it a great potential option for healing of concrete structures.

Elemental Analysis of Reed and Manufacture of Composites Using Mixture of Reed and Poplar

2012 ◽  
Vol 248 ◽  
pp. 243-249
Author(s):  
Xin Zhou Wang ◽  
Jing Wu ◽  
Yu He Deng ◽  
Cheng Bin Liao ◽  
Xiang Lin Fan ◽  
...  
Keyword(s):  
Urea Formaldehyde ◽  
Energy Dispersive Spectrometer ◽  
Physical And Mechanical Properties ◽  
Water Repellent ◽  
Formaldehyde Resin ◽  
Mixing Ratio ◽  
Scanning Electronic Microscopy ◽  
Electronic Microscopy ◽  
Additional Water ◽  
High Silica

Reed is a natural biological material and has great potential as reinforced material in bio-composites.The morphology and element contents of reed stem were investigated using scanning electronic microscopy equipped with energy dispersive spectrometer (SEM/EDS). And the effects of the mass mixing ratio of reed and poplar particles, dosage of water repellent on the physical and mechanical properties of reed/poplar composites were determined. Results indicated that the outside surface of reed stem has high silica content.The mass mixing ratio of 6:4 or 5:5 resulted in an optimum reed/poplar products. As the mass mixing ratio ranged from 4:6 to 10:0, the composites could be produced without adding additional water repellent. Therefore, it is possible to manufacture qualified reed/poplar composites by using urea-formaldehyde resin.

APPLICATION OF SCANNING ELECTRONIC MICROSCOPY FOR THE PURPOSE OF STUDYING CANDIDA ALBICANS BIOFILM ON THE SURFACE OF BASIC PLASTICS OF REMOVABLE ORTHOPEDIC DESIGNS

2019 ◽  
Vol 64 (5) ◽  
pp. 308-313 ◽  
Author(s):  
M. G. Chesnokova ◽  
V. A. Chesnokov ◽  
A. Yu. Mironov
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Candida Albicans ◽  
Electron Microscope ◽  
Biofilm Formation ◽  
Scanning Electronic Microscopy ◽  
Electronic Microscopy ◽  
Orthopedic Rehabilitation ◽  
Candida Albicans Biofilms ◽  
Scanning Electron

The most common pathology in the clinic of orthopedic dentistry is the presence of partial adentia in patients, manifested in the form of defects of dentition of various localization and length. Removable orthopedic structures in the oral cavity are a potential place for adhesion and colonization of microorganisms. The aim of the research was to study Candida albicans biofilms on the surface of base plastics of removable orthopedic structures using scanning electron microscopy. 175 cultures of C. albicans were isolated and identified from the oral mucosa of patients at various stages of orthopedic rehabilitation. When studying the surface of samples of plastics of hot and cold type polymerization and Candida biofilms using a JEOL JCM 5700 scanning electron microscope (JEOL, Japan), features of biofilm formation were established. An assessment of the nature of the manifestation of the hemagglutinating activity of clinical strains of Candida fungi in the hemagglutination test with human erythrocytes I (O), II (A) of the human and guinea pig blood groups was carried out. The total number of hemagglutinating strains was 37.14%, with the prevalence of the proportion of manna-resistant (MRHA) cultures - 23.43% of cases. Micrographs of the C. albicans yeast-like biofilm biofilm were obtained on the surface of hot and cold-type plastics in incubation dynamics. Scanning electron microscopy revealed the most pronounced changes in the surface of hot plastics of polymerization compared to cold plastics with long incubation of C. albicans, which characterize the loosening of plastics and the appearance of cracks on the surface, and the cracking of a yeast-like fungus biofilm was noted.

Micro-Morphology Character and Mineral Composition of PM10 in Nanjing Typical Areas

2012 ◽  
Vol 198-199 ◽  
pp. 81-84
Author(s):  
Qiang Hua Zhang ◽  
Ying Ying Shi ◽  
Qin Zhong
Keyword(s):  
Mineral Composition ◽  
Energy Dispersive Spectrometer ◽  
High Volume ◽  
Scanning Electronic Microscopy ◽  
Electronic Microscopy ◽  
Mineral Particles ◽  
Air Sampler ◽  
Irregular Shapes ◽  
Eds Analysis ◽  
Micro Morphology

The goal of this paper was to study the micro-morphology and mineral composition of PM10 in Nan jing typical areas were studied using scanning electronic microscopy and energy dispersive spectrometer (SEM/EDS). Samples were collected as PM10 for 24 h using high-volume air sampler from Dachang and Shanxilu area. The SEM/EDS analysis showed that mineral particles with regular shapes were popular in PM10 in Dachang District, and mineral particles with irregular shapes were popular in PM10 in Shanxilu area respectively. The composition of mineral particles with regular shapes were mainly carbon ates, sulphates, and aluminosilicates, while mineral particles with irregular shapes were mainly soot aggregates, biomass and primitive minerals.

Observing of Surface Microstructure Pearl in Bionic Materials by Scanning Electron Microscopy

2012 ◽  
Vol 531 ◽  
pp. 379-382 ◽  
Author(s):  
Wei Lin Shi ◽  
Ye Fei Jin ◽  
Qian Xu
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Surface Microstructure ◽  
Scanning Electronic Microscopy ◽  
Electronic Microscopy ◽  
High Quality ◽  
Regular Shape ◽  
Scanning Electron

We present the surface microstructure of pearls in bionic materials investigated by means of scanning electronic microscopy (SEM). We found that surface microstructure of pearls in bionic materials with superior luster and quality are built up by aragonite blocks with regular shape, such as around, polygon or diamond, while pearls high luster are composed of by spindly shaped blocks, and pearls with weak luster and quality are made of some undefined aragonite blocks. Clearly, the regular shaped aragonite blocks are the most idea blocks in crystallography for building high quality pearls. The study of surface microstructure pearl in bionic materials will be helpful for the development of bionic materials.

scholarly journals A Concept for Three-Dimensional Particle Metrology Based on Scanning Electron Microscopy and Structure-from-Motion Photogrammetry

2020 ◽  
Vol 125 ◽  
Author(s):  
Vipin N. Tondare
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Structure From Motion ◽  
3D Model ◽  
Three Dimensional ◽  
Sem Images ◽  
Shape Information ◽  
Size And Shape ◽  
Future Work ◽  
Scanning Electron

Scanning electron microscopy (SEM) has been frequently used for size and shape measurements of particles. SEM images offer two-dimensional (2D) information about a particle’s lateral dimensions. Unfortunately, information about the particle’s three-dimensional (3D) size and shape remains unavailable. To resolve this issue, I propose a new concept in SEM: 3D particle metrology obtained by applying structure-from-motion (SfM) algorithms to multiple rotational SEM images of particles deposited onto a cylindrical substrate to generate a 3D model from which size and shape information can be extracted. Particles can have any size that is suitable for SEM imaging. SEM images of the sample can be acquired from 0° to 360° using a rotational-tip SEM substage. Here, I will discuss the concept and, for clarity, illustrate it with aquarium gravel particles that are glued onto a craft roll and imaged optically before generating the 3D model of that handmade craft. Future work will include the experimental SEM realization, as well as further development of the SfM algorithms. In my view, this proposed concept may become an integral part of SEM-based particle metrology.

ABOUT TRANSLOCATION OF BACTERIA IN PRACTICALLY HEALTHY PEOPLE

2016 ◽  
Vol 1 (61) ◽  
pp. 102-106
Author(s):  
Катола ◽  
Viktor Katola
Keyword(s):  
Blood Plasma ◽  
Healthy People ◽  
Poor Health ◽  
Scanning Electronic Microscopy ◽  
Electronic Microscopy ◽  
Gold Miners ◽  
Translocation Of Bacteria

At research of blood plasma by scanning electronic microscopy in group of 67 practically healthy inhabitants of Blagoveshchensk in 77.6% of cases asymptomatic bacteremia, i.e. translocation of elementary bodies of L-forms bacteria was found out and only in 22.4% of the citizens it was not visualized. The same changes were also revealed in 2 practically healthy gold miners working in difficult environment. Presumably, the translocation of elementary bodies of bacteria occurs owing to the moderate infringement of microbic landscape of thick intestines and insignificant dysfunction of intestines barriers, and it inherently proves poor health of respondents.

Study for Laser Cladding of Ni60A on Copper Substrate by Laser

2008 ◽  
Vol 392-394 ◽  
pp. 125-130 ◽  
Author(s):  
Feng Jie Tian ◽  
Wei Jun Liu ◽  
Xiao Feng Shang ◽  
Guang Yang
Keyword(s):  
Solid Solution ◽  
Laser Cladding ◽  
Erosion Resistance ◽  
Energy Dispersive Spectrometer ◽  
Copper Substrate ◽  
Major Elements ◽  
Bonding Interface ◽  
Scanning Electronic Microscopy ◽  
Electronic Microscopy ◽  
Cladding Layer

The Ni60A powder was clad on the copper by the laser through coaxial power feeding. The hardness and erosion resistance of the substrate and the cladding layer were measured and made contrasts. The morphology and the microstructures of the cladding layer were analyzed by scanning electronic microscopy as well as the microstructure of bonding interface. The contents of major elements in the bonding interface were studied by energy dispersive spectrometer. Moreover, large amount of (Cu and Ni) solid solution formed a metallurgic and tight bonding with the good dilution between the substrate and cladding layer.

Research on the Conductive Property of CNT Nanopaper Facilitates the Actuation in Shape Memory Polymer Composites

2014 ◽  
Vol 887-888 ◽  
pp. 112-115
Author(s):  
A Ying Zhang
Keyword(s):  
Shape Memory ◽  
Shape Memory Polymer ◽  
Three Dimensional ◽  
Scanning Electronic Microscopy ◽  
Electronic Microscopy ◽  
Polycarbonate Membrane ◽  
Dc Voltage ◽  
Multi Walled Carbon Nanotube ◽  
Wide Range ◽  
Conductive Property

A method of synthesizing three-dimensional self-assembled multi-walled carbon nanotube (MWCNT) nanopaper on hydrophilic polycarbonate membrane was investigated. In combination with shape-memory polymer (SMP), the conductive MWCNT nanopaper facilitates the actuation in SMP composite induced by electrically resistive heating. The process is based on the very well-defined dispersion of nanotube and controlled pressure vacuum deposition procedure. The morphology and structure of the nanopaper are characterized with scanning electronic microscopy (SEM) over a wide range of scale sizes. A continuous and compact network observed from the microscopic images indicates that the MWCNT nanopaper could have highly conductive property. The experiment results show that the electrical resistivity of MWCNT nanopaper decreased as temperature increase. SMP composites recovery from a fixed benting shape to the oroginal shape under a constant DC voltage.

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