STUDY OF TOXICOLOGICAL PROPERTIES AND LOCAL IRRITANT EFFECT OF THE DRUGS INSACAR TOTAL C PLUS AND INSACAR TOTAL K PLUS ON LABORATORY RATS

White Rats ◽

Active Substances ◽

The study of acute cutaneous toxicity and local irritant effect of the drugs Insacar Total C Plus and Insacar Total K Plus on mongrel white rats was conducted in doses of 5000 and 10 000 mg/kg of body weight (BW). When the drugs were applied to the skin of rats, no animal deaths were recorded during the entire observation period. Thus, the LD50 is more than 10 000 mg / kg of BW. A single application of the drugs to the skin in rats at a dose of 1 ml/100 g (10 000 units) in two experimental groups on the 7th and 9th day of the study showed the most pronounced irritant effect. In this regard, the drugs have an irritating effect – 1 point. The studied drugs Insacar Total C Plus and Insacar Total K Plus belong to the group of insectoacaricidal and anthelmintic agents. The substances included in its composition have a contact and systemic effect on ectoparasites and nematodes. They are complex medicines containing 4 active components – moxidectin, pyriproxifen, imidacloprid, praziquantel. Such a combination of active substances is characterized by effectiveness against a larger number of parasite species, stages of their development, and in addition, reduce the likelihood of developing resistance of pathogens to drugs.

DETERMINATION OF SAFETY PROFILE OF REPARATIVE ACTIVITY OF THE NEW HYGIENIC PRODUCT “APIPROL”

Wiadomości Lekarskie ◽

10.36740/wlek202108118 ◽

2021 ◽

Vol 74 (8) ◽

pp. 1885-1890

Author(s):

Lyudmila Kravchenko ◽

Elena Appelkhans ◽

Liana Unhurian ◽

Sergey Shcherbakov ◽

Olga Goncharenko ◽

...

Keyword(s):

Antibacterial Properties ◽

Biologically Active ◽

Single Application ◽

Regenerative Processes ◽

Active Components ◽

White Rats ◽

Experimental Trauma ◽

Daily Dose ◽

Further Development

The aim: To prove safety of the new elixir and determine the effect on regenerative processes under conditions of the experimental trauma. Materials and methods: The formula of the new dental elixir “Apiprol” includes biologically active components of bee products (propolis, comb capping wax), plant origin compounds. The elixir was single administered, intragastrally to white nonlinear mice weighing (22±2) g at doses at 50 mg/kg, 500 mg/kg, 5000 mg/kg. Acute toxicity of the new hygienic product “Apiprol” was investigated by a single application at the rate of 1000 and 5000 mg/kg animal weight. Subacute (chronic) toxicity was studied when elixir was applied to the skin of 10 mice at a single daily dose of 500 mg/kg for one month. A local irritant effect of the elixir on the oral mucosa was examined in 14 white rats daily after application for 3–5 min. The study of reparative activity was carried out on an experimental model of the wound process. Results: The elixir’s components promote tissues regeneration, rapid epithelialization and wound surfaces healing, having a highly immune stimulating activity and antibacterial properties. Conclusions: The performed study proved a complete safety of the new elixir “Apiprol” and indicated a necessity of the further development of this preparation in order to create a greater variety of dental means produced in Ukraine.

FILMS IN RUSSIAN MEDICINE AND COSMETOLOGY: DEVELOPMENT HISTORY, CLASSIFICATION, TECHNOLOGY

Pharmacy & Pharmacology ◽

10.19163/2307-9266-2020-8-2-124-132 ◽

2020 ◽

Vol 8 (2) ◽

pp. 124-132

Author(s):

Victoria M. Kishchenko ◽

Vladislav V. Vernikovsky ◽

Igor M. Privalov ◽

Aleksander M. Shevchenko

Keyword(s):

Systemic Effect ◽

Biologically Active ◽

Active Components ◽

Application Form ◽

State Register ◽

History Of ◽

State Of Research ◽

The Moment ◽

Pharmacologically Active ◽

Since the moment of their appearance in the second half of the 20th century, application forms have attracted the attention of the specialists involved in the skin application of pharmacologically active agents. Herewith, both localized exposure to the external integuments and the possibility of achieving a systemic effect, are of interest. The range of products used in modern films, is also wide – from pharmaceutical substances to biologically active components of cosmetics.The aim of the present work is to study the current state of research in the field of the creation and improvement of medicinal and cosmeceutical films.Materials and methods. The study was conducted on the base of patent information (fips.ru, findpatent.ru) and information and search databases - the State register of medicines (grls.rosminzdrav.ru) and the data from the Federal accreditation service (www.fsa.gov.ru), as well as scientific libraries (Google Scholar, eLIBRARY, PubMed) and reference literature.Results. Native and foreign medicinal films have longer than a 50-year history of their existence in the pharmaceutical market. Modern scientists’ interest in this application form, does not fade away due to a great number of its positive characteristics. In addition to pharmaceutical applications, films are widely used in cosmetics in the form of masks applied to the skin. Biologically active substances are widely used in cosmetics which, in recent years, has led to the emergence of a group of cosmeceutical products that combine medical and cosmetic films. The article also discusses film manufacturing technology, active substances, as well as polymers used for medicinal and cosmetic films presented in the Russian market.Conclusion. The analysis of the literature data makes it possible to conclude that the development of films is promising in both medicine and cosmeceuticals.

Toxicological Evaluation of the Complex Insectoacaricidal Preparation "Neoterika Protecto 12"

Russian Journal of Parasitology ◽

10.31016/1998-8435-2018-12-3-60-66 ◽

2018 ◽

Vol 12 (3) ◽

pp. 60-66

Author(s):

E.N. Indyuhova ◽

M.V. Arisov ◽

G.B. Arisova ◽

I.A. Stepanova

Keyword(s):

Aqueous Suspension ◽

Hazardous Substances ◽

Laboratory Animals ◽

Hazard Class ◽

Toxicological Evaluation ◽

Cutaneous Toxicity ◽

Oral Supplementation ◽

Subacute Toxicity ◽

White Rats ◽

The purpose of the research: toxicological evaluation of a medicinal product for veterinary use "Neoterica Protecto 12" on laboratory animals. Materials and methods. White outbred rats, mice and guinea pigs were used in the experiment. In the study of acute oral toxicity in mice and rats, acute cutaneous toxicity, subacute toxicity, irritant action of an aqueous suspension of a combination of active substances, allergenic properties of the collar «Neoterika Protecto 12» conventional methods were used. Results and discussion. The median lethal doses LD50 for oral supplementation to white mice (1070 mg/kg) and oral supplementation to white rats (3210 mg/kg) were diagnosed, which allowed to designate the preparation to the 3rd hazard class (moderately hazardous substances). In the study of acute cutaneous toxicity on white rats LD50 amounted to more than 10 000 mg/kg, therefore, according to the generally accepted hygienic classification, the preparation belongs to the 4th hazard class during cutaneous application. In the study of subacute toxicity of the medication on rats for 6 months after application of the aqueous suspension of the combination of active ingredients in doses of 1000, 500 and 200 mg/kg, no changes in general state and behavior were observed in animals. When an aqueous suspension of a combination of active substances of the collar was applied to the skin of rats, there were no signs of irritant effect, but a mild effect on the mucous membrane of the guinea pig's eyes was recorded. It was found that the preparation does not have allergic and sensitizing effects.

Studies of rootstocks for stone fruit breeds (sweet cherry) in the condition of Hissar valley in Tajikistan

Agricultural Technologies ◽

10.35599/agritech/01.04.05 ◽

2019 ◽

Vol 1 (4) ◽

pp. 28-34

Author(s):

Rahima I. Ismoilova ◽

Sodzhida D. Umarova

Keyword(s):

Sweet Cherry ◽

Sour Cherry ◽

High Specificity ◽

Stone Fruit ◽

Wild Cherry ◽

Phenological Observation ◽

The Individual ◽

Leaf System ◽

This paper is about studying the rootstocks for stone fruit breeds (sweet cherry) in condition of Hissar valley in Tajikistan. Each type of rootstock has its own biological characteristics and imposes specific requirements for growing and development, both during reproduction in the mother plantation and during the growth of trees. For example, the root system in sour cherry is more superficial that of wild sweet cherry. Therefore, the care of trees grafted on sour cherry and wild cherry and of mother plantation bushes of these rootstocks cannot be same. Besides, there are very significant differences among the individual groups of rootstocks. Wild cherry, Mahaleb cherry and Lubskaya cherry are used as rootstocks in the conditions of the Hissar Valley in Tajikistan. High specificity of sweet cherry cultivar varieties depends on the rootstocks. Phenological observation were carried out in our experiments during years 2013-2018 in order to study their winter resistance, yield capacity and fruit quality. The same care for root and grafted plants was carried out during the entire observation period. At the same time a certain ratio between the leaf system of the rootstock and the graft was maintained by trimming the crown. As a result of the evolution and selection, we have identified the wild cherry forms which are distinguished by the highest yields. The most valuable cultivar varieties are Napoleon cherry and Bagration cherry. Compotes made of these varieties have received high evaluation in tasting.

THE INFLUENCE OF SORPTION-DETOXIFYING MEANS ON CUMULATION OF 90SR IN BONE TISSUE OF WHITE RATES WITH COMBINED INTAKE OF 137CS, CD AND PB

Problems of Veterinary Sanitation, Hygiene and Ecology ◽

10.36871/vet.san.hyg.ecol.201803023 ◽

2018 ◽

Vol 1 (3) ◽

pp. 129-133

Author(s):

L. L. Zakharova ◽

◽

G. A. Zhorov ◽

V. N. Obryvin ◽

◽

...

Keyword(s):

Bone Tissue ◽

Chemical Nature ◽

Mechanisms Of Action ◽

The Body ◽

Biologically Active ◽

Laboratory Studies ◽

Animal Body ◽

White Rats ◽

Combined Use ◽

The article presents the results of laboratory studies of the effect of sorption-detoxifying means on the accumulation of 90Sr in the body of white rats. The efficiency of a number of selective and polyfunctional sorbents, detoxicants and other biologically active substances and the developed on their basis sorption-detoxifying complexes as means of efferent therapy and detoxification of the animal body at the combined intake of xenobiotics of radiation and chemical nature was evaluated. It was found that 90Sr cumulation in bones (at the level of 53,3–60,8%) was reduced to the greatest extent with the combined use of substances of different mechanisms of action and origin. The effectiveness of separate use of detoxifying drugs did not exceed 29,1%.

Changes in the occurrence of rainfall-induced landslides in Calabria, Southern Italy, in the 20th century

Natural Hazards and Earth System Sciences Discussions ◽

10.5194/nhessd-3-3579-2015 ◽

2015 ◽

Vol 3 (6) ◽

pp. 3579-3619

Author(s):

S. L. Gariano ◽

O. Petrucci ◽

F. Guzzetti

Keyword(s):

Southern Italy ◽

Geographical Area ◽

Daily Rainfall ◽

Recent Period ◽

Rainfall Events ◽

Rain Gauges ◽

Geographical Variations ◽

The Impact ◽

Abstract. We exploit a catalogue of 1466 rainfall events with landslides in the 90 year period 1921–2010 to study temporal and geographical variations in the occurrence of landslides in Calabria, Southern Italy. We use daily rainfall records obtained by a network of 318 rain gauges to reconstruct 448 493 rainfall events. Combining the rainfall and the landslide information, we obtain a catalogue of 1466 rainfall events with landslides (REL) in Calabria from 1921 to 2010, where a REL is the occurrence of one or more landslide during or immediately after a rainfall event. We find that the geographical and the temporal distributions of the rainfall-induced landslides have changed in the observation period. The average and the maximum values of the cumulated event rainfall that have resulted in landslides in the recent-most 30 year period 1981–2010 are lower than the values necessary to trigger landslides in previous periods, whereas the duration of the rainfall events that triggered landslides has remained the same. This can be considered evidence of variations in rainfall conditions, but also an increase in the vulnerability of the territory. We further find that the yearly distribution of rainfall-induced landslides has changed in the observation period, analysing the variations in the number of rainfall events with landslides occurred in each month in three 30 year periods. To investigate variations in the impact of REL on the population, we compared the number of REL in each of the 409 municipalities in Calabria, with the size of the population in the municipalities, measured by national Censuses conducted in 1951, 1981, and 2011. For the purpose, we adopted two strategies. The first strategy considered impact as IREL = #REL/P and the second strategy measured impact as RREL = #REL × P, where #REL is the total number of REL in a period, and P is the size of the population in the same period and geographical area. Considering the entire observation period, IREL and RREL have both increased in Calabria. However, considering the changes between the recent period 1981–2010 and the previous period 1951–1980, results are more variegated with a number of municipalities where IREL and RREL have increased, or decreased. Municipalities where IREL has increased are mainly in the mountains, and municipalities where RREL has increased are mainly along the coasts.

EFFECT OF LOCAL UV RADIATION ON THE MORPHOFUNCTIONAL STATE OF THE SKIN OF GUINEA PIGS IN EARLY AND REMOTE PERIODS AFTER IRRADIATION

Wiadomości Lekarskie ◽

10.36740/wlek202004115 ◽

2020 ◽

Vol 73 (4) ◽

pp. 705-707

Author(s):

Tetyana V. Zvyagintseva ◽

Svitlana I. Myronchenko ◽

Nataliia I. Kytsiuk ◽

Olga V. Naumova

Keyword(s):

Ultraviolet Irradiation ◽

Guinea Pigs ◽

Acute Inflammation ◽

Elastic Fibers ◽

Control Group ◽

Morphological Picture ◽

Morphometric Methods ◽

The aim is to establish the features of morphological and morphometric changes in the skin of guinea pigs in erythemal, early post-erythemal and late post-erythemic periods after local ultraviolet irradiation. Materials and methods: Studies were conducted on 54 albino guinea pigs weighing 400-500 g. Ultraviolet erythema was caused by irradiation in 1 minimum erythemal dose. The control group included intact guinea pigs. After 2, 4 hours, on the 3rd, 8th, 15th, 21st, 28th day, the fragments of the irradiated skin were investigated using histochemical and morphometric methods. Results: After 2, 4 hours after irradiation, dyscirculatory changes in the skin develop. By the 3rd day of the experiment a morphological picture of acute inflammation in the epidermis and dermis develops, apoptotic keratinocytes appear (sunburn cells), which is accompanied by thickening of the epidermis and an increase in the density of fibroblasts. By the 8th day proliferative-hyperplastic and degenerative changes begin to prevail, including dystrophic nature, the thickness of the epidermis and the density of fibroblasts reach a maximum. In the long term, on the 15-28th day, dystrophic changes of the epidermis, dyskeratosis, changes in the number and structure of elastic fibers with an increase in uneven fibrosis, collagenization processes and the development of sclerotic changes, as well as a significant thickening of the epidermis, an increase in the density of fibroblasts are observed. Conclusions: The data obtained indicate pronounced morphofunctional changes in the skin in the zone of local ultraviolet irradiation observed throughout the entire observation period.

Active Substances from Callicarpa nudiflora Exert Anti-Cervicitis Effects and Regulate NLRP3-Associated Inflammation

Molecules ◽

10.3390/molecules26206217 ◽

2021 ◽

Vol 26 (20) ◽

pp. 6217

Author(s):

Tianchi Liu ◽

Ruiqi Wang ◽

Chenpeng Liu ◽

Jiahong Lu ◽

Yitao Wang ◽

...

Keyword(s):

Chinese Medicine ◽

Ethanol Extract ◽

Inflammatory Factor ◽

Potential Mechanism ◽

Active Components ◽

New Agents ◽

Scientific Support ◽

Luohuazizhu suppository is a Traditional Chinese Medicine used in clinic to treat cervicitis, which is prepared from Callicarpa nudiflora Hook. et Arn (C. nudiflora), an herbal Chinese medicine named Luohuazizhu. This study aimed to figure out the active constituents of C. nudiflora and the potential mechanism for its anti-cervicitis effect. The ethanol extract in C. nudiflora (CNE) and the different fractions of CNE extracted by petroleum ether (CNE-p), dichloromethane (CNE-d), and n-butanol (CNE-b) were tested in vivo for their anti-cervicitis effects. Then the isolated compounds from the CNE-p were tested in vitro for their anti-inflammatory activities. The results displayed that CNE-p, CNE-d, and CNE-b exhibited adequate anti-cervicitis effects, with CNE-p showing the highest efficacy. Further experiment demonstrated that CNE-p could significantly inhibit the expression of NLRP3 in vitro. Six diterpenoids obtained from the CNE-p showed the ability to regulate inflammatory factor levels in vitro. Among these compounds, compounds 1 (callicarpic acid A) and 2 (syn-3,4-seco-12S-hydroxy-15,16-epoxy-4(18),8(17),3(16),14(15)-labdatetraen-3-oic acid) were the most effective agents, and they also inhibited the expression level of NLRP3 in vitro. The results confirmed that C. nudiflora has significant anti-cervicitis effects and the diterpenoids were most likely to be its active components. These data provide scientific support for the clinic usage of Luohuazizhu suppository and the development of new agents in treating cervicitis.

CONSERVATIVE TREATMENT OF HEMORRHOIDS. AN ALTERNATIVE TO SURGICAL METHODS OR COMPONENTS? CHORUS PROGRAM RESULTS

Koloproktologia ◽

10.33878/2073-7556-2018-0-1-27-35 ◽

2018 ◽

pp. 27-35

Author(s):

E. A. Zagryadskiy ◽

A. M. Bogomazov ◽

E. B. Golovko

Keyword(s):

Surgical Treatment ◽

Conservative Treatment ◽

Clinical Manifestations ◽

Surgical Methods ◽

Micronized Purified Flavonoid Fraction ◽

Flavonoid Fraction ◽

Observational Group ◽

Grade Iii ◽

OBJECTIVE. Determine the frequency of clinical manifestations of hemorrhoids and constipation in people seeking advice about hemorrhoids. In the course of the treatment of patients with hemorrhoids phlebotropic evaluate the effectiveness of therapy micronized purified flavonoid fraction (MPFF). MATERIALS AND METHODS. This multicenter study, including screening and observation part, which is part of the International Research «CHORUS» (Chronic venous and hemorrhoid diseases evaluation and scientific research), conducted in nine centers in different regions of Russia, 80 doctors of Coloproctology. In the screening group included 2668 patients who had investigated the incidence of constipation, as a risk factor for hemorrhoids. Conservative treatment, the foundation of which was, Moffitt therapy, received 1952 patients with stage I-IV hemorrhoids. Evaluating the effectiveness of the treatment was evaluated on the basis of a questionnaire. RESULTS. The questionnaire shows that constipation suffered - 766 (28,8 %) patients. Violation of defecation patterns and changes in stool consistency was observed in 1155 (43,9%) and 633 (25.5 %), respectively. At the same time, 288 (11,1 %) indicated a tendency to loose stools and diarrhea. Conservative treatment, the foundation of which is phlebotropic MPFF therapy conducted in patients of observational group has shown its efficiency in all grades of hemorrhoids. During the entire observation period of conservative treatment was effective in 1489 (76,3 %) patients. Surgical treatment was performed in 463 (23 %) patients grade I-IV hemorrhoids, the main part of patients with grade III -199 (43,1 %) and grade IV hemorrhoids - 68 (64,2 %). CONCLUSION. Conservative treatment of hemorrhoid disease, which is the basis on phlebotropic MPFF therapy, is effective at all stages of hemorrhoids, but in patients with grade III and grade IV disease requires surgical treatment.

The role of brief intervention in achieving and maintaining abstinence in patients with alcoholic liver disease

Medical Council ◽

10.21518/2079-701x-2019-21-182-188 ◽

2020 ◽

pp. 182-188

Author(s):

V. D. Lunkov ◽

M. V. Maevskaya ◽

V. T. Ivashkin

Keyword(s):

Liver Disease ◽

Alcoholic Liver Disease ◽

Psychological Intervention ◽

Decompensated Cirrhosis ◽

Control Group ◽

Factors Associated ◽

Group A ◽

Objective of the study. prove the effectiveness of brief psychological intervention (BPI) conducted by an internist in achieving and maintaining abstinence in patients with alcoholic liver disease (ALD).Materials and methods. A total of 65 patients were included in the study: 29 patients in the BPI group and 36 in the historical control group. A comparative analysis of the frequency of achievement and maintenance of abstinence and analysis of factors associated with these parameters were conducted.Results of the study. The frequency of achieving abstinence was significantly higher in the BPI group compared with the control group after 6, 9, 12 and 24 months from the date of inclusion in the study (p <0.001, p = 0.002, p = 0.001, p = 0.017, respectively; criterion χ2). The frequency of failures to achieve abstinence in the CPC group was significantly lower than in the control group after 6 months and in general for the entire observation period (p = 0.004, p = 0.005, respectively; criterion χ2). Provision of BPIs for 12 months after alcohol-induced decompensation serves as a factor that is reliably associated with achieving total abstinence within 24 months (p = 0.001, criterion χ2). Decompensated cirrhosis of the liver serves as factors independently associated with failures to achieve abstinence within 24 months after alcohol-induced illness (OS: 10.72 [95% CI 2.17–52.81]; p = 0.004) and the absence of BPI after discharge from the hospital (OSH BPI: 0.80 [95% CI 0.14–0.479]; p = 0.006)Conclusion. BPIs provided by an internist to the patients with ABD for 12 months after alcohol-induced decompensation leads to a higher rate of achieving total abstinence and decrease in the frequency of failures to achieve abstinence within 24 months after discharge from the hospital.