EFFECT OF THE FODDER ADDITIVE GEMOVIT-MEYAN ON THE CONTENT OF LEAD AND CADMIUM IN THE BODIES AND TISSUES OF WHITE RATS IN SUBACUTE EXPERIMENT

2019 ◽  
Vol 1 (4) ◽  
pp. 456-460
Author(s):  
V.I. Dorozhkin ◽  
◽  
G.I. Pavlenko ◽  
N.A. Brichko ◽  
N.S. Pavlova ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Feed Additive ◽  
Potential Effectiveness ◽  
Additional Tool ◽  
Lead And Cadmium ◽  
White Rats ◽  
Animal Organs

When white rats are poisoned with lead and cadmium at a dose of 50 and 5 mg/ kg of feed, the largest accumulation of heavy metals is observed in the liver (4,04 mg/kg Pb and 6,13 mg/kg Cd) and kidneys (4,13 mg/kg Pb and 9,09 mg/ kg Cd), a rather high content of lead was also noted in the spleen (3,77 mg/kg). The use of the gemovit-meyan feed additive for 2 months on the background of poisoning led to a decreasing in the content of cadmium in all the studied animal organs by 40...60 %, while for lead there was only a tendency to reduce its content in the organs. On the basis of the obtained data, it is possible to conclude about the potential effectiveness of gemovit-meyan in poisoning with heavy metals and recommend its further study as an additional tool that can reduce heavy metals intoxication.

scholarly journals Effect of Diatomite on the Accumulation of Lead and Cadmium in the Body of White Rats

2021 ◽  
Vol 37 ◽  
pp. 00144
Author(s):  
Vasily I. Dorozhkin ◽  
Natalia A. Brichko ◽  
Natalia S. Pavlova ◽  
Galina I. Pavlenko
Keyword(s):  
Heavy Metals ◽  
Animal Husbandry ◽  
Cadmium Accumulation ◽  
The Body ◽  
Feed Additive ◽  
Lead And Cadmium ◽  
White Rats ◽  
Accumulation Of Metals ◽  
Livestock Products ◽  
Cadmium And Lead

Every year, pollution by eco-toxicants of natural and anthropogenic origin is becoming more and more acute. Some of the most toxic among them are heavy metals, especially cadmium and lead. These elements do not decompose. Once released into the environment, these elements continue to circulate in water, soil, and air for a very long time, accumulating in plants and livestock products. Therefore, there is a problem of obtaining safe agricultural products in areas contaminated with heavy metals. In animal husbandry, synthetic and natural enterosorbents are used for obtaining safe products. For example, such enterosorbents are shungites, bentonites, diatomites, clays. We tested diatomite from the Kamyshlovskoye deposit at a dose of 5.0 g/kg of feed to reduce cadmium accumulation and lead during their combined intake. We investigated the feed intake of a combination of cadmium and lead at doses of 5.0 and 50.0 mg/kg feed. The result of this experiment is the accumulation of metals in the liver, kidneys, and bones, and, in addition, in the testes. The use of diatomite to reduce the accumulation of heavy metals has not been successful. There is an increase in lead accumulation in the liver, kidneys, and testes. The content of cadmium in diatomite is 3.86 ± 0.20 mg/kg, lead – 105.0 ± 7.2 mg/kg. The results obtained indicate that diatomite as a feed additive to reduce lead and cadmium intoxication did not show protective properties.

EFFECT OF L-CYSTEINE ON THE ACCUMULATION OF CADMIUM AND LEAD IN THE BODY OF WHITE RATS

2020 ◽  
Vol 1 (3) ◽  
pp. 378-383
Author(s):  
V.I. Dorozhkin ◽  
◽  
G.I. Pavlenko ◽  
N.A. Brichko ◽  
N.S. Pavlova ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
The Body ◽  
Feed Additive ◽  
Heavy Metal Poisoning ◽  
Maximum Accumulation ◽  
Lead And Cadmium ◽  
White Rats ◽  
Metal Poisoning ◽  
Cadmium And Lead

The feed additive L-cysteine ​​in a therapeutic dose was used for 2 months against the background of poisoning of white rats with a combination of lead and cadmium at a level of 10 MPC (50 and 5 mg / kg, respectively). The maximum accumulation of heavy metals was found in the kidneys (Pb up to 8,15 mg / kg, Cd 2,51 mg / kg) and liver (Pb up to 7,44 mg / kg, Cd 1,80 mg / kg) of rats. With the introduction of L-cysteine ​​into the feed, the content of lead decreased by 2 times and cadmium by 2 ... 4 times in all investigated organs. Thus, L-cysteine has shown a fairly complete protective effect in heavy metal poisoning and can be recommended as a means that can reduce heavy metal (HM) intoxication.

THE RESULTS OF STUDYING THE EFFICIENCY OF DIATOMITE AT INTOXICATION OF ANIMALS WITH CADMIUM AND LEAD

2021 ◽  
Vol 1 (2) ◽  
pp. 214-222
Author(s):  
V.I. Dorozhkin ◽  
◽  
G.I. Pavlenko ◽  
N.S Pavlova ◽  
D.A. Drozdov ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Body Weight ◽  
Blood Serum ◽  
Feed Additive ◽  
Protective Properties ◽  
Sh Groups ◽  
Lead And Cadmium ◽  
White Rats ◽  
Cadmium And Lead ◽  
Heavy Metal Intoxication

The combination of cadmium and lead in doses at the level of 10 MPC for feed caused in white rats a decrease in body weight and immunoglobulin content in blood serum, an increase in the summation threshold indicator, a decrease of the amount of protein in urine and SH-groups in blood serum, a significant increase in mass coefficients of the liver and kidneys. The use of diatomite as a means to reduce heavy metal intoxication slightly increased body weight. The use of means did not lead to normalization of the summation threshold indicator, did not significantly affect the content of SH-groups. In the group of animals treated with diatomite, the mass coefficients of the liver remained significantly higher than the control values. The obtained results indicate that diatomite did not show protective properties as a feed additive to reduce lead and cadmium intoxication.

scholarly journals Effect of sodium selenite and feed additive “Metisevit plus” on morphological parameters of blood of rats at the intoxication of Cadmium and Lead

2020 ◽  
Vol 22 (97) ◽  
pp. 52-57
Author(s):  
S. О. Slobodian ◽  
B. V. Gutyj ◽  
S. D. Murska
Keyword(s):  
Heavy Metals ◽  
Sodium Selenite ◽  
Hemoglobin Level ◽  
The Body ◽  
Lead Intoxication ◽  
Feed Additive ◽  
Morphological Parameters ◽  
Lead And Cadmium ◽  
Cadmium And Lead ◽  
Experimental Group

Heavy metals, Lead and Cadmium, are hazardous environmental pollutants. In the body of mammals, Cadmium and Lead have a toxic effect on a number of organs and systems. The mechanism of action of these poisons is to inhibit the activity of enzyme systems by blocking carboxyl, amine and SH groups of protein molecules. The aim of the study was to investigate the effect of sodium selenite and feed additive “Metisevit plus” on the morphological parameters of rat blood by Cadmium and Lead intoxication. The experiments were carried out on 200–220 g male Wistar rats, out of which 3 groups of animals were formed: a control and two experimental animals. Rats in the control and experimental groups were administered 16.6 % aqueous lead acetate at a dose of 100 mg/kg and 0.029 % aqueous cadmium chloride at a dose of 2.0 mg/kg. The rats of the first experimental group additionally used sodium selenite. The rats of the second experimental group additionally used the feed additive “Metisevit plus”. Lead-cadmium loading in rats was accompanied by erythrocytopenia and leukopenia. The lowest erythrocyte count was in the blood of diseased rats at 21 days, where it decreased by 41.3 %, respectively, compared to the initial values. It was found that hemoglobin level decreased to 90.3 ± 2.63 g/l during Cadmium and Lead intoxication in control rats. At the same time as the decrease in hemoglobin level and the number of erythrocytes in intoxicated rats, an increase in the average hemoglobin content in one erythrocyte of blood was observed up to 20.57 ± 1.20 pg As a result of the development of lead-cadmium intoxication in ill rats, leukopenia was noted, which, in our opinion, is associated with systemic lesions of cadmium and lead in the blood-forming organs of animals that suppress leukopoiesis. It was found that the number of leukocytes in patients of rats of group C decreased by 36.1 % compared with the initial data. The use of sodium selenite and the feed additive “Metisevit plus” in rats under lead-cadmium loading contributed to the increase in the number of red blood cells and hemoglobin level, as well as the decrease in the number of leukocytes in their blood. The improvement of normalizing effect on the morphological parameters of blood of rats under conditions of loading with heavy metals was shown by the feed additive “Metisevit plus” in comparison with sodium selenite.

Polymorphism of p-450 cytochrome detoxication genes in adolescents depending on the degree of contamination of the organism by heavy metals

2020 ◽  
Vol 99 (5) ◽  
pp. 478-482
Author(s):  
N. P. Setko ◽  
A. G. Setko ◽  
Ekaterina V. Bulycheva ◽  
A. V. Tyurin ◽  
E. Yu. Kalinina
Keyword(s):  
Heavy Metals ◽  
Environmental Factors ◽  
Heavy Metal Contamination ◽  
Atomic Absorption Spectrophotometry ◽  
The Body ◽  
Lead And Cadmium ◽  
Cyp2b6 Gene ◽  
Cytochrome P 450 ◽  
Gene Cyp1a1 ◽  
Group 1

Introduction. Changes in the body of children and adolescents aimed at adapting to environmental factors are determined by genetic polymorphism in xenobiotic biotransformation genes, determining the degree of susceptibility of the child’s body to pollutants, which is the basis of modern personalized preventive medicine when managing risks to the health of the child population under the influence of environmental factors. Material and methods. Trace elements, including heavy metals, lead and cadmium, were determined in the hair of 256 practically healthy teenagers by atomic absorption spectrophotometry. Depending on the level of content of the latter, two groups of adolescents were formed to determine six genes of the cytochrome P-450 family. Group 1 consisted of adolescents whose cadmium lead content exceeded the average Russian indices. The second group included adolescents whose heavy metals were above the level of average Russian standards. Results. Studies have shown that in adolescents of the 1st group, compared with the data of adolescents of the 2nd group, an increase in the number of carriers of two mutant alleles at the locus rs 1048943 (gene CYP1A1) is 3.08 times, rs 464621 (gene CYP1A1) is 1. 8 times; locus rs 2069522 (CYP1A2 gene) 3.63 times; locus rs 1799853 (CYP2C9 * 2 gene) 4.5 times; locus rs 1057910 (gene CYP2C9 * 3) 3.8 times and locus rs 2279343 (gene CYP2B6) 4.25 times. Moreover, carriers of two normal alleles in adolescents of the first group at the locus rs 1048943 (gene CYP1A1) were 5.14 times; locus rs 2279343 (CYP2B6 gene) was 6.5 fold less than among adolescents of the 2nd group; and at the locus rs 464621 (gene CYP1A1), rs 2069522 (gene CYP1A2), rs 1799853 (gene CYP2C9 * 2), rs 1057910 (gene CYP2C9 * 3) there were no carriers of normal homozygotes. Conclusion. Group 1 adolescents with heavy metal contamination of the body are carriers significantly in a greater number of pathological mutations in the genes of the cytochrome P-450 detoxification system in comparison with data from group 2 adolescents.

Determination of Some Heavy Metals in Milk of Cow Grazing At Selected Areas, of Kaduna Metropolis

2017 ◽  
Vol 7 (7) ◽  
pp. 341 ◽  
Author(s):  
Mahmud Mohammed Imam ◽  
Zahra Muhammad ◽  
Amina Zakari
Keyword(s):  
Heavy Metals ◽  
Cadmium Toxicity ◽  
Research Work ◽  
The Body ◽  
Cow Milk ◽  
Scientific Model ◽  
Potential Health ◽  
Milk Samples ◽  
Lead And Cadmium ◽  
Digestion Technique

In this research work the concentration of zinc, copper, lead, chromium, cadmium, and nickel in cow milk samples obtained from four different grazing areas   (kakuri, kudendan, malali, kawo) of Kaduna metropolis. The samples were digested by wet digestion technique .The trace element were determined using bulk scientific model VPG 210 model  Atomic Absorption Spectrophotometer (AAS).. The concentration of the determined heavy metal were The result revealed that Cr,  Ni and Cd were not detected in milk samples from Kawo, Malali  and Kudendan whereas lead (Pb) is detected in all samples and found to be above  the stipulated limits of recommended dietary allowance (NRC,1989) given as 0.02mg/day. Cu and Zn are essential elements needed by the body for proper metabolism and as such their deficiency or excess is very dangerous for human health. However, they were found in all samples and are within the recommended limits while Cd (2.13 – 3.15 mg/kg) in milk samples from Kakuri was found to be above such limit (0.5mg/day). Cow milk samples analyzed for heavy metals in this research work pose a threat of lead and cadmium toxicity due to their exposure to direct sources of air, water and plants in these grazing areas, thereby, resulting to a potential health risk to the consumers.

Pollution retention capabilities of roadside soils

1999 ◽  
Vol 39 (2) ◽  
pp. 201-208 ◽  
Author(s):  
C. Dierkes ◽  
W. F. Geiger
Keyword(s):  
Heavy Metals ◽  
Water Quality ◽  
Drinking Water ◽  
Groundwater Recharge ◽  
Drinking Water Quality ◽  
Traffic Density ◽  
Roadside Soils ◽  
Highway Runoff ◽  
Lead And Cadmium ◽  
Lead Zinc

Runoff from highways contains significant loads of heavy metals and hydrocarbons. According to German regulations, it should be infiltrated over embankments to support groundwater-recharge. To investigate the decontaminating effect of greened embankments, soil-monoliths from highways with high traffic densities were taken. Soils were analyzed to characterize the contamination in relation to distance and depth for lead, zinc, copper, cadmium, PAH and MOTH. Lysimeters were charged in the field and laboratory with highway runoff to study the effluents under defined conditions. Concentrations of pollutants in roadside soils depend on the age of embankments and traffic density. Highest concentrations were found in the upper 5 cm of the soil and within a distance of up to two metres from the street. Concentrations of most pollutants decreased rapidly with depth and distance. Lead and cadmium could not be detected in lysimeter effluent. Zinc and copper were found in concentrations that did not exceed drinking water quality limits.

scholarly journals Paper-based electroanalytical devices for stripping analysis of lead and cadmium in children's shoes

RSC Advances ◽  
2020 ◽  
Vol 10 (68) ◽  
pp. 41482-41487
Author(s):  
Chen-Chen Zhu ◽  
Ning Bao ◽  
Xiao-Lei Huo
Keyword(s):  
Heavy Metals ◽  
Toxic Heavy Metals ◽  
Stripping Analysis ◽  
Lead And Cadmium ◽  
Potential Sources

Children's shoes are potential sources of toxic heavy metals, especially for younger children.

scholarly journals Use of Post-Extraction Fir Wood Greenery Residues by the Bioconversion Method with the Production of Feed Additives

Forests ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 272
Author(s):  
Olga O. Mamaeva ◽  
Elena V. Isaeva
Keyword(s):  
Heavy Metals ◽  
Weight Loss ◽  
Maximum Permissible Concentration ◽  
Forest Resources ◽  
Feed Additives ◽  
Feed Additive ◽  
Pleurotus Pulmonarius ◽  
Balsam Poplar ◽  
Fallen Leaves ◽  
Protein Feed

The effectiveness of forest resources depends on the comprehensiveness and rationality of their consumption and processing into finished products. This article discusses the problem of using solid fir wood greenery residues generated during the industrial production of essential oils. Bioconversion is considered to be the most promising use. The objective of this research was to study the chemical composition of bioconversion products of fir wood greenery-based substrates. The РР-3.2 strain of Pleurotus pulmonarius (Fr.) Quél was used as a biodestructor. In the process of bioconversion, the contents of polysaccharides and lignin substances is reduced to 38% and 28%, respectively. Up to 20% of protein accumulates in bioconversion products of fir wood greenery. The amount of nucleic acids is not more than 1.5 g per kg; the contents of heavy metals, such as mercury, cadmium, arsenic, and lead, do not exceed the maximum permissible concentration standards. The substrate weight loss reaches 15%. When fallen leaves and post-extraction poplar bud residues are added to the substrate, the substrate-destroying activity of fungi increases, and the protein content increases by 3%. The digestibility of products as a result of bioconversion increases 1.6–2.8 times depending on the substrate composition. The obtained data support the recommendation of post-fermented substrates based on fir wood greenery and balsam poplar biomass for use as a protein feed additive.

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