scholarly journals Chemical constituents and bactericidal and fungicide potential of the essential oil of Pimenta dioica Lindl against pathogenic microorganisms

2021 ◽  
pp. 112-121
Author(s):  
Gustavo O. Everton ◽  
Ana Patrícia M. Pereira ◽  
Nilton S. C. Mafra ◽  
Paulo S. Santos Júnior ◽  
Thércia G. Martins ◽  
...  
Keyword(s):  
Essential Oil ◽  
Chemical Constituents ◽  
Dilution Method ◽  
Pathogenic Microorganisms ◽  
E Coli ◽  
Fusarium Sp ◽  
Pimenta Dioica ◽  
Antimicrobial Assay ◽  
Broth Dilution ◽  
Potential Use

This study evaluated the chemical profile and antimicrobial activity of essential oil (EO) of P. dioica. The EOs were extracted by hydrodistillation and chemically characterized by gas chromatography coupled to mass spectrometry (GC/MS). The total phenolics were quantified by the Folin Ciocalteu method. For the antimicrobial assay, the Disc Diffusion and Broth Dilution method were applied to obtain the minimum inhibitory concentration and minimum bactericidal concentration. The main constituent of the EO was eugenol. The EO showed bactericidal activity against E. coli, S. aureus, P. aeruginosa, Salmonella sp., B. cereus, P. mirabilis, K. pneumoniae, S. sonnei, C. albicans, Fusarium sp., Penicillium sp. and Aspergilus sp. The results obtained are encouraged by the potential use of the EO studied in the control and combat of pathogenic microorganisms.

scholarly journals Amtimicrobial activity of essential oil of Melissa officinalis L, Lamiaceae

2005 ◽  
Vol 59 (9-10) ◽  
pp. 243-247 ◽  
Author(s):  
Nada Anicic ◽  
Suzana Dimitrijevic ◽  
Mihailo Ristic ◽  
Slobodan Petrovic ◽  
Slobodan Petrovic
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Bacterial Species ◽  
Antimicrobial Properties ◽  
Dilution Method ◽  
Melissa Officinalis ◽  
E Coli ◽  
Diffusion Technique ◽  
Main Components ◽  
Broth Dilution

The antimicrobial activity of the essential oil of Melissa officinalis was investigated in this paper. The essential oil was obtained by the principle of water and steam and analyzed by GC and GC-MS using FID and MSD. The main components of the oil of Melissa officinalis were geranial (17.30%), neral (14.70%) and citronellal (10.70%). The antimicrobial properties were tested against the following bacterial species: B subtilis, B.cereus Bifidobacterium sp., Corynobacterium sp., E. coli, Klebsiella sp., L. acidophilus, L. fermentum, L. plantarum, L. rhamnosus, Listeria monocytogenes, P. vulgaris, P. aerugenosa, S. enteritidis, Shigella sp., S. aureus, and fungi Candida albicans, Alternarija sp. and Aspergillus niger. The diffusion technique was used for testing: the antimicrobial activity, and the MIC was determined by the broth dilution method. The essential oil of M. officinalis showed high antimicrobial activity.

scholarly journals Chemical Composition and Antimicrobial Activities of the Essential Oil From the Leaves of Pterocephalus hookeri

2020 ◽  
Vol 15 (12) ◽  
pp. 1934578X2098123
Author(s):  
Peng-fei Yang ◽  
Hui Lu ◽  
Qiong-bo Wang ◽  
Zhi-wei Zhao ◽  
Qiang Liu ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Chemical Constituents ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Flame Ionization ◽  
And Staphylococcus Aureus ◽  
Antimicrobial Assay ◽  
Detailed Chemical

Detailed chemical constituents of essential oil from the Pterocephalus hookeri leaves and its antimicrobial activities were investigated in this study. The essential oil, obtained by hydrodistillation, was characterized by gas chromatography-flame ionization detection and gas chromatography-mass spectrometry analyses. Among the 90 identified compounds, hexadecanoic acid (21.27%), phytol (8.03%), furfural (7.08%), oleic acid (5.25%), and phytone (4.56%) were the major components. In the antimicrobial assay, the essential oil showed strong inhibitory activities against Escherichia coli, Candida albicans, and Staphylococcus aureus with minimum inhibitory concentration values of 31.3, 62.5, and 125 µg/mL, respectively. To our knowledge, this is the first report concerning chemical composition and antimicrobial activities of the essential oil from Pterocephalus hookeri.

scholarly journals A Facile Synthesis and Studies of Some New Chalcones and Their Derivatives Based on Heterocyclic Ring

2012 ◽  
Vol 9 (4) ◽  
pp. 1897-1905 ◽  
Author(s):  
A. Solankee ◽  
K. Patel ◽  
R. Patel
Keyword(s):  
Inhibitory Concentration ◽  
Guanidine Hydrochloride ◽  
Heterocyclic Ring ◽  
Dilution Method ◽  
Facile Synthesis ◽  
Phenyl Hydrazine ◽  
Gram Negative ◽  
E Coli ◽  
Heterocyclic Aldehydes ◽  
Broth Dilution

Chalcones(6a-f)have been prepared by the condensation of ketone(5)and different aromatic and heterocyclic aldehydes. These chalcones(6a-f)on treatment with guanidine hydrochloride and phenyl hydrazine hydrochloride in presence of alkali give aminopyrimidines(7a-f)and phenylpyrazolines(8a-f)respectively. All the newly synthesized compounds have been characterized on the basis of IR,1HNMR spectral data as well as physical data. Antibacterial activity (minimum inhibitory concentration MIC) against Gram-positiveS. aureusMTCC 96 andS. pyogeneusMTCC 442 and Gram-negativeP. aeruginosaMTCC 1688 andE. coliMTCC 443 bacteria, as well as antifungal acivities (MIC) againstC. albicansMTCC 227,A. nigerMTCC 282 andA. clavatusMTCC 1323 were determined by broth dilution method.

scholarly journals IN VITRO MICROBIAL TIME-KILLING CURVE FOR NEWLY SYNTHESIZED AMINOACETYLENIC-2-MERCAPTOBENZOTHIAZOLE COMPOUND

2017 ◽  
Vol 9 (10) ◽  
pp. 125
Author(s):  
Aseel Alsarahni ◽  
Zuhair Muhi Eldeen ◽  
Elham Al-kaissi ◽  
Hiba Al-malliti
Keyword(s):  
Minimum Bactericidal Concentration ◽  
Viable Count ◽  
Broth Culture ◽  
Dilution Method ◽  
Time Intervals ◽  
Minimum Fungicidal Concentration ◽  
E Coli ◽  
Different Types ◽  
Broth Dilution

Objective: To determine the time needed for killing different types of microorganisms by a newly synthesized 2-mercapto-1,3-benzothiazole derivative in comparison to ciprofloxacin and fluconazole.Methods: The minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC) for 2-{[4-(2,6-dimethylPiperidin-1-yl)but-2-yn-1-yl]Sulfanyl}-1,3-benzothiazole(AZ3) compound were determined, using the broth dilution method. The MBC and MFC dilutions were prepared. Broth cultures of Staphylococcus aureus (S. aureus), Bacillus subtilis (B. subtilis), Escherichia coli (E. coli), and Pseudomonas aeruginosa (P. aeruginosa) were incubated at 37 °C for 24 h, and Candida albicans (C. albicans) was incubated at 25 °C for 48 h. 0.1 ml of each broth culture represent 1.5 x 106 CFU/ml was challenged with 9.9 ml broth containing the MBC or MFC concentrations of the AZ3 compound. From each sample at different time intervals, 1 ml was taken and added to 9 ml of sterile distilled water, in order to neutralize the effect of AZ3. Serial dilution was done and a viable count was determined from the appropriate dilutions.Results: The viability of the P. aeruginosa, E. coli, S. aureus, B. subtilis and C. albicans were killed within 3.5 h, 5 h, 24 h, 3 h and 5 h respectively. The time killing curves showed that AZ3 needed longer time for killing S. aureus than the time needed to kill B. subtilis. On the other hand, AZ3 needed a shorter time to kill P. aeruginosa, than the time needed to kill E. coli. In comparison with ciprofloxacin, AZ3 needed a shorter time to kill P. aeruginosa and E. coli, and the same time to kill B. subtilis, while it needed longer time than ciprofloxacin to kill S. aureus. In comparison with fluconazole, AZ3 with lower MFC than fluconazole needed longer time to kill C. albicans.Conclusion: AZ3 showed promising antimicrobial killing activities, in compared with ciprofloxacin and fluconazole, which promoted our interest to investigate the time of killing needed for other 2-mercaptobenzothiazole derivatives against different types of microorganisms.

scholarly journals Isolation and Antimicrobial Activity of β-Sitosterol-3-O-Glucoside from Lannea Kerstingii Engl. & K. Krause (Anacardiacea)

2016 ◽  
Vol 06 (01) ◽  
pp. 004-008
Author(s):  
Njinga N. S. ◽  
Sule M. I. ◽  
Pateh U. U. ◽  
Hassan H. S. ◽  
Abdullahi S. T. ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Wide Spectrum ◽  
Diffusion Method ◽  
Dilution Method ◽  
Minimum Fungicidal Concentration ◽  
E Coli ◽  
H Nmr ◽  
Vacuum Liquid Chromatography ◽  
Broth Dilution ◽  
C Nmr

AbstractThe emergence of more and more drug resistance bacteria has led to the study of the antimicrobial activity of the compound isolated from Lannea kerstingii Engl. & K. Krause (Anacardiacea) since the active principles of many drugs found in plants are secondary metabolites. A compound was isolated using dry vacuum liquid chromatography and eluting with CHCl3 -EtOAc and monitored using TLC. 3 1 13 The glycoside was characterized using 1 H NMR and 13 C NMR spectra recorded in DMSO-d6 at 400 MHz and 125 MHz, respectively. The antimicrobial activity of the compound was determined using agar diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal/minimum fungicidal concentration (MBC/MFC) was determined using broth dilution method. The compound isolated was found to be β-sitosterol-3-O-glucoside. The β-sitosterol-3-O-glucoside (200μg/ml) was active against S. aureus, Methicillin Resistant Staphylococcus aureus, P. mirabilis, S. typhi, K. pneumoniae, E. coli, B. subtilis with zone of inhibition ranging from 24mm to 34mm and inactive against P. aeroginosa and Proteus vulgaris. It was also active against the fungi C. albicans and C. tropicalis but inactive against C. krusei. The MIC ranged from 25 to 50 μg/ml while the MBC/MFC ranged from 50 to 200 μg/ml. These results show the wide spectrum antimicrobial activity of β-sitosterol-3-O-glucoside.

scholarly journals Microbial Contamination of Chicken Litter Manure and Antimicrobial Resistance Threat in an Urban Area Setting in Cameroon

Antibiotics ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 20
Author(s):  
Marie Paule Ngogang ◽  
Tambo Ernest ◽  
Jennifer Kariuki ◽  
Mohamed Moctar Mouliom Mouiche ◽  
Jeanne Ngogang ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Dilution Method ◽  
Salmonella Spp ◽  
E Coli ◽  
Chicken Litter ◽  
Convenience Sampling ◽  
And Control ◽  
Broth Dilution

We conducted a pilot study to assess microbiological safety of chicken litter, an affordable organic and main fertilizer used in Cameroon and worldwide. A convenience sampling of 26 farms was done and a questionnaire was administered. Samples of litter were aseptically collected. E. coli and Salmonella spp. were isolated using CLSI standards. Antibiotic susceptibility testing was performed using the disc diffusion method and a micro broth dilution method for colistin. In broiler farms, 90% of participating farmers gave antibiotic prophylaxis. The prevalence of E. coli and Salmonella spp. was 59.1% and 15.5%, respectively. All E. coli isolates were multidrug resistant as well as 36.4% for Salmonella spp. No resistance was found against cefepime and imipenem. All Salmonella spp. tested were found sensitive to colistin while 26.7% of E. coli spp. were colistin resistant. Contamination of chicken litter may be an underestimated source of antimicrobial resistance (AMR) transmission towards animals, humans and the environment with multidrug resistant E. coli and Salmonella spp. This shows the need and opportunity for a One Health approach in AMR surveillance and control in Cameroon. Continued surveillance in chicken litter would enable monitoring of AMR risks and trends.

scholarly journals VOLATILE COMPOSITION, ANTIOXIDANT PROPERTY AND ANTIMICROBIAL ACTIVITIES AGAINST FOOD-BORNE BACTERIA OF VIETNAMESE THYME (Thymus vulgaris L.) ESSENTIAL OIL

2019 ◽  
Vol 57 (3B) ◽  
pp. 127 ◽  
Author(s):  
Cung Thi To Quynh ◽  
Vu Thu Trang
Keyword(s):  
Essential Oil ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Thymus Vulgaris ◽  
Dilution Method ◽  
Volatile Components ◽  
E Coli ◽  
Food Borne ◽  
Inhibition Zones ◽  
Bacteria And Fungi

The essential oil (EO) obtained from the leaves of the thyme (Thymus vulgaris L.) grown in Vietnam was found to contain thymol (39.79%), cymene (17.33%), and γ-terpinene (13.45%) as the main volatile components. The antimicrobial activities of this oil were screened against several food-borne bacteria and fungi species. Significant growth inhibition effects against food-borne bacteria Escherichia coli, Staphylococcus aureus, Bacillus cereus, and Salmonella Typhimurium were observed using the standard disc diffusion method. Thyme EO showed the antibacterial effect against all the test pathogenic strains with the inhibition zones were 23.3 ± 0.4 mm, 24.7 ± 0.4 mm, 29.0 ± 0.7 mm, 32 ± 0.7 mm in diameter against B. subtilis,  E. coli, S. Typhimurium and S. aureus, respectively. The minimum inhibitory concentration (MIC) determined by micro-dilution method in MHB liquid medium was 1.56 µl/ml. The bactericidal concentrations (MBC) was 3.13 µl/ml for three isolates from B. subtilis,  E. coli, and S. aureus, while the MBC tested for S. typhi was 1.56 µl/ml. The antifungal properties of the thyme EO were also determined in this study against three important pathogenic fungi such as Candida albicans, Rhizoctonia solani and Fusarium oxysporum with the inhibition zones ranging approximately from 23.20 ± 0.06 to 44.10 ± 0.03 mm. On the other hands, the results also showed the antioxidant activity of Vietnamese thyme EO and suggested that thyme EO can be applied in food industries as natural flavoring preservatives/additives to control food spoilage and food born bacteria and fungi.

scholarly journals Antimicrobial activity of berries and leaves essential oils of Macedonian Juniperus foetidissima Willd. (Cupressaceae)

2015 ◽  
Vol 61 (01) ◽  
pp. 3-11 ◽  
Author(s):  
Floresha Sela ◽  
Marija Karapandzova ◽  
Gjose Stefkov ◽  
Ivana Cvetkovikj ◽  
Elena Trajkovska-Dokikj ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Essential Oil ◽  
Essential Oils ◽  
Dilution Method ◽  
Moderate Activity ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Germacrene D ◽  
Broth Dilution

Chemical composition and antimicrobial activity of leaves and berries essential oils from Juniperus foetidissima Willd. (Cupressaceae) grown in R. Macedonia (RM) was investigated. GC/FID/MS analysis was carried out and 93 components were identified, representing 89.7-96.5% of the oils. The major components of the berries essential oil were α-pinene (19.2%), limonene (24.9%) and cedrol (23.1%), followed by smaller amounts of b-funebrene, trans-caryophyllene, germacrene D and d-cadinene. The composition of the leaves essential oil was variable depending on the region of collection. Accordingly, samples originated from southeastern RM contained essential oil with α-pinene (67.6%) and limonene (10.0%), from central part of RM with limonene (17.9-27.1%) and cedrol (28.8-33.9%), while samples from southwestern RM contained oil with terpinen-4-ol (19.1%), cis-thujone (8.3%), germacrene D (11.0%) and d-cadinene (6.3%) as predominant components in the oil. Antimicrobial screening of the essential oils was made by disc diffusion and broth dilution method against 16 bacterial strains of Gram-positive and Gram-negative bacteria and one strain of Candida albicans. The leaves essential oil showed stronger antimicrobial activity against Staphylococcus aureus, Streptococcus pyogenes and Haemophilus influenzae (MIC = 125 ml/ml) and moderate activity against Campylobacter jejuni (MIC > 500 ml/ml). Other investigated bacterial strains and Candida albicans were completely resistant to the antimicrobial activity of J. foetidissima essential oils.

Design, Synthesis and Antimicrobial Study of Novel 1-(1,3-benzothiazol-2- yl)-3-chloro-4H-spiro[azetidine-2,3'-indole]-2',4(1'H)-diones Through Ketene– imine Cycloaddition Reaction

2020 ◽  
Vol 17 (2) ◽  
pp. 141-148
Author(s):  
Divyani Gandhi ◽  
Ayushi Sethiya ◽  
Dinesh Kr. Agarwal ◽  
Prakash Prajapat ◽  
Shikha Agarwal
Keyword(s):  
Candida Albicans ◽  
Antimicrobial Agents ◽  
Cycloaddition Reaction ◽  
Antimicrobial Activities ◽  
Dilution Method ◽  
Bacterial Strains ◽  
Design Synthesis ◽  
E Coli ◽  
Excellent Activity ◽  
Broth Dilution

: The present study deals with the synthesis of novel 1-(1,3-benzothiazol-2-yl)-3-chloro-4Hspiro[ azetidine-2,3'-indole]-2',4(1'H)-dione derivatives from the reaction of 3-(1,3-benzothiazol-2- ylimino)-1,3-dihydro-2H-indol-2-one derivatives with chloroacetyl chloride in the presence of triethylamine (TEA). The mechanism involved simple acid or base catalysed reaction through the formation of Schiff base followed by cyclisation via ketene–imine cycloaddition reaction. All synthesized compounds were characterized by FT-IR, 1H-NMR, 13C-NMR, and elemental analysis. The antimicrobial activities of the synthesized derivatives 5a-5g were examined via Micro Broth Dilution method against bacterial strains Bacillius subtilis, Staphylcoccus aureus, E. coli, P. aeruginosa, and fungal strain Candida albicans for determining MIC values. Ampicillin, chloramphenicol, and griseofulvin were used as standard drugs. : The MIC values for antimicrobial activity of synthesized compounds were examined using Micro Broth Dilution method. Compounds 5a, 5b, and 5c were found effective against E. coli (MTCC 442) and P.aeruginosa (MTCC 441) and all compounds showed moderate to excellent activity against Streptococcus aureus (MTCC 96) and Bacillius subtilis (MTCC 441). Regarding the antifungal screening, compounds 5a, 5b, and 5c exhibited excellent activity against Candida albicans MTCC 227. 1-(1,3-benzothiazol-2-yl)-3-chloro-4H-spiro[azetidine-2,3'-indole]-2',4(1'H)-dione derivatives may be used as potential lead molecules as effective antimicrobial agents.

scholarly journals Preparation of the Essential Oil from Artemisia Argyi Grown in Qichun, China and its application in Antibacterial effection

2020 ◽  
Vol 189 ◽  
pp. 02016
Author(s):  
Hui Hu ◽  
Qingan Li ◽  
Shenxi Chen ◽  
Yuancai Liu ◽  
Huameng Gong ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Chemical Constituents ◽  
Volatile Oil ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Components ◽  
Dilution Method ◽  
Artemisia Argyi ◽  
Coating Method ◽  
Potential Applications

To evaluate the antibacterial activity and chemical constituents of the essential oil from the artemisia argyi grown in Qichun (China). METHODS: Steam distillation method was used to extract volatile oil from Artemisia argyi. The antibacterial effect of the volatile oil was investigated by the plate coating method and the double gradient liquid dilution method. Gas chromatography-mass spectrometry(GC-MS) was applied for the identification of chemical constituents in volatile oil from Artemisia argyi and the relative percentage of each component was calculated by area normalization. RESULTS: The essential oil from artemisia argyi grown in Qichun (China) has significant antibacterial activity against staphylococcus aureus, pseudomonas aeruginosa, salmonella, candida albicans, aspergillus niger and aspergillus flavus. And fifty chemical components were detected in the essential oil, and twenty compounds were identified, accounting for 95.95% of total essential oil. And the artemisol in artemisia argyi grown in Qichun (China) was found to be the highest compared with the same species from other producing areas. CONCLUSION: The essential oil from artemisia argyi grown in Qichun (China) was a potent antibacterial plant extract with potential applications as an antibacterial drugs or food preservative.

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