scholarly journals STANDARDIZATION AND TOXICOLOGICAL STUDIES OF THE LEAF OF SENNA SIAMEA IRWIN & BARNEBY (FABACEAE) COLLECTED FROM AGULU IN AWKA SOUTH OF ANAMBRA STATE

2021 ◽  
Vol 3 (01) ◽  
pp. 25-36
Author(s):  
Chinasa Charity Ezea
Keyword(s):  
Methanol Extract ◽  
Proximate Analysis ◽  
Water Soluble ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Standard Methods ◽  
Toxicological Effect ◽  
Toxicological Effects ◽  
Toxicological Studies ◽  
Phytochemical Constituents

Background: Pharmacognostic standards and toxicological studies of the leaf of S. siamea were carried out. Objectives: To determine the quantitative phytochemical constituents present, pharmacognostic standards and  toxicological effect of the methanol leaf extract and fractions of S. siamea. Methodology: Fresh leaves of S. siamea were collected, dried, pulverizes to powder and subjected to pharmacognostic and quantitative phytochemical analysis. The pulverized leaves were cold macerated in methanol for three days with intermittent shaking and exchange of fresh solvent every 24 hrs (aliquot extraction). Fractionation was done using n-hexane, ethylacetate and butanol. Chromatographic analysis of the methanol extract and fractions were obtained using standard methods. The methanol extract and fractions where screened for phytochemical constituents and toxicological effects using standard methods. Acute toxicity (LD50) of the extract was determined  using Lorke’s method. Results: Macroscopic and microscopic examinatios revealed acute apex, pinnate veination and diacytic stomata, trichomes respectively. Phytochemical screening of the extract and fractions revealed the presence of flavonoids, alkaloids, saponins, tannins etc. Proximate analysis of S. siamea revealed moisture content of 5.50 %, total ash value of 15.00 %, water soluble ash of 2.00 % etc.  The LD50 was found to be > 5000 mg/kg. Increase in ALT, AST and ALP when metanol extract and fractions were administered were not statistically different from the control group, indicating no hepatotoxic potential. Increase in PCV, Hb and RBC when metanol extract and fractions were administered were not statistically different from control group. The non-significant decrease in the levels of ALP, ALT and AST indicates that the extract has possible hepatoprotective effect. The non-significant decrease in the levels of RBC, Hb and PCV indicates that the extract does not affect the heamatopoietic system when administered orally and at the doses used in the study. Conclusions: Specific standards obtained from the study may be compiled into a monograph of S.siemea plant that may constitute in herbal pharmacopoeia.

scholarly journals PHYSICOCHEMICAL AND PHYTOCHEMICAL ANALYSIS OF Mangifera indica linn.( AAMRA KERNEL )

2020 ◽  
Vol p4 (05) ◽  
pp. 2394-2400
Author(s):  
Pooja Dogra ◽  
A. Rama Murthy ◽  
Sumit Nathani
Keyword(s):  
Mangifera Indica ◽  
Natural Antioxidants ◽  
Water Soluble ◽  
Phytochemical Analysis ◽  
Tropical Regions ◽  
Potential Source ◽  
Mango Kernel ◽  
Phytochemical Constituents ◽  
Phytochemical Studies

Introduction: Mangifera Indica Linn., Anacardiaceae family, is a large evergreen tree of tropical and sub-tropical regions of the world. Mango kernel can be used as a potential source for functional food ingredi-ents, animal feeds, antimicrobial compounds and cosmetic due to its high quality of fat and protein as well as high levels of natural antioxidants. Aim: The aim of the present study was to evaluate the physicochemical and phytochemical analysis of Mangifera Indica Linn. Methods Aamra Kernel were collected, cleaned, dried and powdered. Aqueous and alcoholic extracts of Aamra kernel were analyzed for physicochemical and phytochemical constituents. The study was conduct-ed as per the guidelines of Ayurvedic pharmacopeia of India. Results: The result of physicochemical parameters (moisture content: 10.2%, total ash: 2.12%, acid insolu-ble ash: 0.3%, water soluble ash: 1.42%, aqueous extractive value: 14.67% and alcoholic extractive value 12.98%). The phytochemical studies revealed the presence of active constituents, carbohydrates, amino acid, steroids, proteins, saponins, alkaloids, glycosides, tannins in aqueous and alcoholic extracts. Conclusion: All studies standardization parameters like physicochemical and phytochemical screening pa-rameters provide the knowledge in the identification, authentication of kernel of Mangifera Indica Linn. Hence it is concluded that this drug supposed to be great beneficial and boon for our society.

scholarly journals In vitro and in vivo anti-trypanosomal potentials of Afrormosia laxiflora and Khaya senegalensis against Trypanosoma brucei brucei

2018 ◽  
Vol 39 (3) ◽  
pp. 269-284
Author(s):  
G.D. Chechet ◽  
J Yahaya ◽  
A.J. Nok
Keyword(s):  
Body Weight ◽  
Trypanosoma Brucei ◽  
Post Infection ◽  
Methanol Extract ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Trypanosoma Brucei Brucei ◽  
Khaya Senegalensis

Animal African trypanosomiasis (AAT) also known as Nagana is a resurgent disease in Africa. Medicinal plants are being used in less developed countries for the treatment of various diseases including trypanosomiasis, due to the high cost of currently available drugs. Most of these plants have been useful sources of treatment of various diseases based on information obtained from folk medicine but have not been scientifically certified. Here, we investigated the in vitro and in vivo anti-trypanosomal potentials of the methanol extract of Aformorsia laxiflora and Khaya senegalensis against T. b. brucei. Phytochemical screening as well as LD50 of the plant extracts was carried out following standard procedures. Parasitemia was monitored daily while Packed Cell Volume was determined at three time points (days 1, 4 and 7) during the course of the infection. The phytochemical analysis showed the presence of saponins, alkaloids, flavonoids, antraquinones, resins and tanins. However, steriods/terpenoids were absent in K. senegalensis but present in A. laxiflora. The toxicity of methanol extract of both A. laxiflora and K. senegalensis was above 5000mg/kg body weight. Methanol extracts of A. laxiflora (leaves) and K. senegalensis (stem bark) showed promising trypanocidal potential in vitro against T. b. brucei at concentrations of 10, 15, 25mg/ml and 40 and 20mg/ml respectively. At these concentrations, both extracts immobilized the parasites within 55mins post-incubation. In general, A. laxiflora leaf extract demonstrated prophylactic activity against T. b. brucei in vivo at a dose of 500mg/Kg body weight particularly in group C animals where a delayed pre-patent period (6 days post-infection), extended survival (14 days post-infection) and significant (P<0.05) reduction in the parasite burden confirmed by an absence of anemia (PCV 47.00±0.8 %) was observed when compared to the infected untreated control group. K. senegalensis extract on the other hand did not show anti-trypanosomal activity in the treated groups (1, 2, and 3). Based on these observations, it was therefore deduced that the methanol extract of leaves of A. laxiflora possessed the ability to ameliorate the burden of the disease and could be a plausible candidate for drug development against the disease.Keywords: Trypanosoma brucei brucei, Afromosia laxiflora, Khaya senegalensis, anti-trypanosomal, in vitro, in vivo

scholarly journals Comparative Analysis on the Phytochemical, Proximate and Mineral Composition of the Seeds and Peels of Lime

2021 ◽  
Vol 46 (2) ◽  
Author(s):  
D. Uzama ◽  
S. C. Okolo ◽  
R. U. Okoh-Esene ◽  
A. B. Adebiyi ◽  
A.T. Orishadipe
Keyword(s):  
Comparative Analysis ◽  
Mineral Composition ◽  
Crude Protein ◽  
Proximate Analysis ◽  
Phytochemical Analysis ◽  
Mineral Analysis ◽  
Volatile Oils ◽  
Crude Fibre ◽  
Standard Methods ◽  
Proximate And Mineral Composition

Comparative Analysis on the Phytochemical, proximate and mineral composition of the seeds and peels of lime was carried out. The phytochemical, and proximate screenings were carried out using standard methods. The mineral analysis was carried out by using Atomic Absorption Spectrophotometer. The proximate analysis revealed that the seeds contain Moisture 8.20%, Ash 12.09%,Crude fat 8.50%,Crude Protein 1.93%,Crude fibre 20.71%, and Carbohydrate 48.58% while the peels contain Moisture10.60%, Ash 15.27%, Crude fat14.67%, Crude Protein 2.30%, Crude fibre23.36% and Carbohydrate 33.80%.The phytochemical analysis revealed the presence of alkaloid, saponins, flavonoids, terpenoids, phenols, and volatile oils in both the seeds and peels of the lime. In addition, the seeds contain steroids, while the peels contain tannins, balsams and anthraquinone. The mineral analysis revealed that the seeds contains Ni(0.17mg/100g),Pb(0.01mg/100g),Mn (0.20mg/100g),Zn(0.57mg/100g)Cr(0.17mg/100g),Mg(9.02mg/100g),Ca(27.02mg/100g),Cd(0.01mg/100g), Fe(2.55mg/100g) and Cu(4.81mg/100g) while the peels contain Ni(0.15mg/100g),Pd(0.21mg/100g),Mn(0,21mg/100g),Zn(0.23mg/100g),Cr(0.13mg/100g),Mg(7.61mg/100g),Ca(100.22mg/100g),Cd(0.01mg/100g),Fe(1.67mg/100g) and Cu(0.21mg/100g).The lime seeds and peels can serve as potential sources of drugs and nutrition with the seeds having higher concentration of minerals. 

scholarly journals ANTIMICROBIAL ACTIVITY OF LEMON PEEL (CITRUS LIMON) EXTRACT

2017 ◽  
Vol 9 (4) ◽  
pp. 79 ◽  
Author(s):  
Junab Ali ◽  
Biswajit Das ◽  
Trideep Saikia
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Citrus Limon ◽  
Phytochemical Analysis ◽  
Bacterial Strains ◽  
Gram Negative ◽  
Standard Methods ◽  
Phytochemical Constituents

Objective: To evaluate the antimicrobial activity of methanolic extract from the peel of the fruit of Citrus Limon (Family-Rutaceae) in conjugation with phytochemical analysis.Methods: The methanolic extract from the peel of the fruit of Citrus Limon (Family-Rutaceae) was separated from fruits, shade dried, powdered and extracted using methanol, analysed for phytochemical constituents using standard methods. The antimicrobial activity of the plant extract was examined against 2 bacterial strains among one is Gram-positive (Staphylococcus aureus) and other is Gram-negative (Escherichia coli) and 1 fungal strains (Candida albicans) using agar well diffusion method.Results: The present investigation shows the phytochemical analysis, antimicrobial activity of the methanolic extract of the fruits peel Citrus limon. Various phytochemical analyses revealed the presence of alkaloids, saponin, flavonoids, carbohydrates, glycosides and citric acids and tannins. The antimicrobial activity of the methanolic extract of the plant showed significant result against all the of the test organisms.Conclusion: The present study concluded that methanolic extract of the peel of Citrus Limon contains the high presence of phytochemicals. The methanolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards. 

scholarly journals PRELIMINARY PHYTOCHEMICAL ANALYSIS AND ANTIMICROBIAL ACTIVITY ETHANOLIC EXTRACTS OF DRIED FRUITS OF SOLANUM TORVUM (FAMILY-SOLANACEAE)

2017 ◽  
Vol 9 (3) ◽  
pp. 123
Author(s):  
Lawrence Kalita ◽  
Biswajit Dash ◽  
Uttam Borah ◽  
Juman Deka ◽  
Suvakanta Dash
Keyword(s):  
Antimicrobial Activity ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Bacterial Strains ◽  
Solanum Torvum ◽  
Dried Fruits ◽  
Standard Methods ◽  
Aerial Parts ◽  
Ethanolic Extracts ◽  
Phytochemical Constituents

Objective: To study the antimicrobial activity ethanolic extracts of dried fruits of Solanumtorvum (family-solanaceae) along with preliminary phytochemical analysis.Methods: The ethanolic extract of dried fruits of the herb Solanumtorvum (family-solanaceae) were prepared and analyzed for phytochemical constituents using standard methods. The antimicrobial activity of the plant extracts were examined against 2 bacterial strains among one is gram positive and other is gram negative and 2 fungus using agar well diffusion method.Results: The present experiment shows the phytochemical analysis, antimicrobial activity of the ethanolic extract of dried fruits of the plant Solanumtorvum (family-solanaceae). Various phytochemical analysis revealed the presence of alkaloids, saponin, flavonoids, carbohydrates, glycosides, Steroids, proteins and amino acids and tannins. All the extracts of the plant showed antimicrobial activity against most of the test organisms.Conclusion: It can be concluded ethanolic extract of whole aerial parts of the Solanumtorvum (family-solanaceae) contain the high presence of phytochemicals. This extract was found to possess promising antimicrobial activity when compared with the standards.

scholarly journals Toxicological Effect of Euphorbia heterophylla Linn

2021 ◽  
pp. 1-15
Author(s):  
J. K. Kone ◽  
A. K. Onifade
Keyword(s):  
White Blood Cells ◽  
Clinical Manifestations ◽  
Negative Control Group ◽  
Negative Control ◽  
Hot Water ◽  
Control Group ◽  
Albino Rats ◽  
Toxicological Effect ◽  
Toxicological Effects ◽  
Animal House

Euphorbia heterophylla has been reported to possess enormous medicinal benefits hence there is a need for holistic research on its toxicological effects. Four different solvents (cold and hot water, chloroform and 80% methanol) were used to extract the aerial parts of Euphorbia heterophylla Linn. Experimental albino rats were obtained from Federal University of Technology Akure’s Microbiology Department Animal House. Toxicological analysis of Euphorbia heterophylla extracts was carried out on the experimental albino rats. The animals were grouped into thirteen with three rats in a group different concentrations of the extracts were administered orally to different groups of the rats for a period of two weeks (14 days). Sterile distilled water was administered to the negative control group. During the 14 days of extracts administration, the animals were observed for clinical manifestations like salivation, nervousness, imitability, itching of the nose and diarrhoea. After the expiration of the two weeks, the animals were sacrificed and vital organs like heart, liver, kidney and intestine were harvested and examined for any degenerative changes and lesions. Blood samples were collected before and after the administration of the extracts to check for blood parameters (Packed Cell Volume (PCV), white blood cell count (total and differential), erythrocyte sedimentation rate (ESR) and haemoglobin (Hb) estimation. The toxicological results of the extracts showed various degrees of abnormalities detected in the histopathological sections of the intestine, kidney and the heart. It was found that all the extracts at different concentrations burnt the intestinal walls of all the animals tested. There were significant reductions in the total white blood cells (WBC) and neutrophil counts while there was a significant increase in the lymphocytes count after the administration of the extracts. The findings of this study revealed that Euphorbia heterophylla had toxicological effects on the albino rats organs but increases there lymphocytes count, boosting their immune system, however advance researches to eliminate the toxic phytochemicals from the extract is recommended.

Phytochemical, Quantitative Proximate and In vitro Anti-inflammatory Study of the Crude Methanol Extract of Stachytarpheta indica Leaves (Verbenaceae)

2019 ◽  
pp. 153-162
Author(s):  
Cyril Ogbiko ◽  
Dickson Achimugu Musa ◽  
Musa Usman Dabai ◽  
Ibeabuchi Jude Ali ◽  
Abubakar Sani Yelwa ◽  
...  
Keyword(s):  
Cardiac Glycosides ◽  
Methanol Extract ◽  
Proximate Analysis ◽  
Water Soluble ◽  
Inflammatory Activity ◽  
Dependent Manner ◽  
Anti Inflammatory ◽  
Dose Dependent ◽  
Crude Methanol Extract

Herbal medicine despite its increase usage globally is being plagued by inadequate research on their effectiveness. Stachytarpheta indica commonly known as snakeweed is used locally in the management of asthma, headache, alopecia, bronchitis, bruise, chest cold, constipation, itch, diarrhea, skin sore, vermifuge, dysentery, dysmenorrhea among others. This study investigated the phytochemical composition, quantitative proximate parameters and in vitro anti-inflammatory activity of the leaves of S. indica using established methods. Results confirmed the presence of alkaloids, saponins, carbohydrate, cardiac glycosides, terpenoid, tannin, anthraquinones, phenolics and flavonoid. Moisture content (9.16 ± 0.04%), total ash (19.42 ± 0.15%), water insoluble ash (7.17 ± 0.22%), acid insoluble ash (3.63 ± 0.26%), alcohol soluble extractive (0.99 ± 0.06%) and water-soluble extractive (0.36 ± 0.02%) values were obtained from the quantitative proximate analysis. An infusion of the methanol leaf extract of Stachytarpheta indica demonstrated significant anti-inflammatory activity in a dose dependent manner comparable to that of indomethacin standard.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACT OF WHOLE AERIAL PART OF THE HERB LEUCAS PLUKENETII SPRENG (FAMILY-LAMINACEAE)

2017 ◽  
Vol 9 (3) ◽  
pp. 87
Author(s):  
Uttam Borah ◽  
Biswajit Dash ◽  
Suvakanta Dash ◽  
Lawrence Kalita
Keyword(s):  
Antimicrobial Activity ◽  
Aerial Part ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Bacterial Strains ◽  
Standard Methods ◽  
Aerial Parts ◽  
Phytochemical Constituents

Objective: To evaluate the antimicrobial activity of ethanolic extract of Leucas Plukenetii Spreng (family-laminaceae) in conjugation with phytochemical analysis.Methods: The ethanolic extract of whole aerial parts of the herb Leucas Plukenetiispreng (family-laminaceae) was prepared and analysed for phytochemical constituents using standard methods. The antimicrobial activity of the plant extract was examined against 2 bacterial strains among one is gram positive and other is gram negative and 2 fungal strains using agar well diffusion method.Results: The present investigation shows the phytochemical analysis, antimicrobial activity of the ethanolic extract of the plant Leucas Plukenetii Spreng. Various phytochemical analyses revealed the presence of alkaloids, saponin, flavonoids, carbohydrates, glycosides, Steroids, proteins and amino acids and tannins. The antimicrobial activity of the ethanolic extract of the plant showed significant result against all the of the test organisms.Conclusion: The present study concluded that ethanolic extract of whole aerial parts of the Leucas Plukenetii Spreng contains the high presence of phytochemicals. The ethnaolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards.

scholarly journals Phytochemical Analysis and Antimalarial Activity Aqueous Extract ofLecaniodiscus cupanioides Root

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Mikhail Olugbemiro Nafiu ◽  
Taoheed Adedeji Abdulsalam ◽  
Musbau Adewumi Akanji
Keyword(s):  
Body Weight ◽  
Aqueous Extract ◽  
Antimalarial Activity ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Weight Group ◽  
Body Weights ◽  
Phytochemical Constituents ◽  
The Mean ◽  
Group 1

Root aqueous extract ofLecaniodiscus cupanioideswas evaluated for antimalarial activity and analyzed for its phytochemical constituents. Twenty-four (24) albino mice were infected by intraperitoneal injection of standard inoculum of chloroquine sensitivePlasmodium berghei(NK 65). The animals were randomly divided into 6 groups of 3 mice each. Group 1 served as the control while groups II–IV were orally administered 50, 150, and 250 mg/kg body weights of extract. Groups 5 and 6 received 1.75 and 5 mg/kg of artesunate and chloroquine, respectively. The results of the phytochemical analysis showed the presence of alkaloids (2.37%), saponin (0.336), tannin (0.012 per cent), phenol (0.008 per cent), and anthraquinone (0.002 per cent). There was 100 per cent parasite inhibition in the chloroquine group and 70 per cent in the 50 mg/kg body weight on day 12, respectively. The mean survival time (MST), for the control group was 14 days, artesunate 16 days, and chloroquine 30 days, while the groups that received 50 and 250 mg/kg body weight recorded similar MST of 17 days and the 150 mg/kg body weight group recorded 19 days. The results obtained indicated that the aqueous extract ofLecaniodiscus cupanioidesmay provide an alternative antimalarial.

scholarly journals Pharmacognostic Standardization, Phenolic and in Vitro Antioxidant Activity of Silene villosa (Family: Caryophyllaceae)

2017 ◽  
Vol 9 (3) ◽  
pp. 106 ◽  
Author(s):  
Ahmed I. Foudah
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Antioxidant Property ◽  
Water Soluble ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Microscopic Features

The present study was designed to explore some of the quality principles and <em>in vitro</em> antioxidant exploration of <em>Silene villosa</em> “a non-intentional” herb. The pharmacognostical study and phytochemical evaluation of the herbs of <em>S. villosa</em> was carried out following the reported methods. The total phenolic contents and antioxidant potential of the methanol extract was also evaluated applying the standard methods. Microscopic features of the Transverse Section (T.S) of the root were observed as epidermis, medullary rays, xylem cells, phloem cells. The microscopic study of the powder showed the presence of spiral vessels, lignified fibre or tannin containing cells. The extractive value showed the water soluble materials were higher than the alcohol soluble materials. Primarily phytochemical analysis and total phenolic contents (11.78±0.169 %) in powder and extract respectively were conducted. The methanol extract of <em>S. villosa</em> showed dose dependent antioxidant activity. The present study helps in authentication of <em>S. villosa</em> and enhances further exploration of the health benefits due to the presence of phenolic contents and antioxidant property.

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