scholarly journals DISTRIBUTION OF PCN PATHOTYPES IN POLAND

2019 ◽  
pp. 3-8
Author(s):  
Anna Przetakiewicz
Keyword(s):  
Potato Production ◽  
Globodera Rostochiensis ◽  
Soil Samples ◽  
Potato Cultivars ◽  
Globodera Pallida ◽  
European Countries ◽  
Regional Survey ◽  
Potato Crops ◽  
Starch Potato

Both Globodera rostochiensis (Wollenweber, 1923) Behrens, 1975 and Globodera pallida (Stone, 1973) are considered the most economically important nematode pest. Annual regional survey conduct in Poland have revealed high infestation of pathotype Ro1 to the northwest of the country and presence of new pathotype Ro5 of G. rostochiensis. In 2009-2013 fifty eight soil samples were tested. parasitic root organisms cause significant losses in quantity and quality of potato crops. In many European countries PCN have become a major problem in potato production mainly in starch potato cultivars. The pathotype Ro1 was detected mostly in central and northern Poland and pathotype Ro5 in four separated provinces: malopolskie, lubuskie, lubelskie and pomorskie. The abundance of cysts in location where the new pathotype was detected suggests that is a thread of further spread of pathotype Ro5 of G. rostochiensis in the country. Consequently, producers and farmers need to grow resistant potato cultivars to keep nematode population below damaging level. These results indicate the urgent need to prevent the spread of PCN to non-infested areas. This report focused on identification of nematode pathotypes in soil samples from infested polish fields and presentation of G. rostochiensis distribution on territory of Poland.

scholarly journals First Report of the Pale Potato Cyst Nematode Globodera pallida from Bosnia and Herzegovina

Plant Disease ◽  
2014 ◽  
Vol 98 (4) ◽  
pp. 575-575 ◽  
Author(s):  
B. Nježić ◽  
B. Gerič Stare ◽  
S. Širca ◽  
N. Grujić
Keyword(s):  
Bosnia And Herzegovina ◽  
Potato Production ◽  
Cyst Nematode ◽  
Soil Samples ◽  
Potato Cyst Nematode ◽  
Globodera Pallida ◽  
Economic Losses ◽  
Cyst Nematodes ◽  
Seed Potatoes ◽  
Infested Field

Potato cyst nematodes (PCN), the golden cyst nematode Globodera rostochiensis (Woll.) Bahrens, and the pale potato cyst nematode G. pallida (Stone) stand out by their remarkable and efficient parasitic adaptations causing high economic losses in potato production worldwide. They are A2 quarantine pests in EPPO countries as well as in Bosnia and Herzegovina. Up to now, only G. rostochiensis was reported from Bosnia and Herzegovina in 2011 (1). A systematic survey on the presence of PCN on entire territory of Bosnia and Herzegovina started in 2011. During the PCN survey, each year 120 soil samples of 1.5 liters were collected in the Republic of Srpska, one of the two entities of Bosnia and Herzegovina. The samples were collected by soil auger 10 cm in length with a diameter of 1.5 cm consisting of 100 cores. Entire samples were processed by Seinhorst elutriator (4). In autumn of 2012, PCN viable cysts were found in two soil samples originating from one field. From one sample, 12 cysts were extracted, and 2 from another sample. The morphology of cysts and second stage juveniles and molecular analysis established the identity of this species as pale cyst nematode G. pallida (3). In addition, the sequencing of the ribosomal DNA region revealed unequivocal similarity to G. pallida (GenBank Accession No. HF968449), while PCR-RFLP analysis (2) showed European type of G. pallida. The infested field is located in Rogatica, 70 km east of Sarajevo, which is one of the main seed and potato production areas in Bosnia and Herzegovina. The field history revealed that farm-propagated, farm-saved seed potatoes could spread the nematodes to other fields as well. Therefore, 26 additional samples were taken from the fields that belong to the infested field owner and the surrounding fields, but no cysts were found in additional samples. To determine infestation focus and its size, the infested field (1.1 ha) was divided into 46 plots (25 × 10 m) and resampled by taking samples of 60 cores per plot. The detailed sampling of the field revealed a high infestation of 1 cyst per gram of soil in the infestation focus. The high infestation level and the propagation of farm-own seed potatoes suggest that the introduction of G. pallida might have occurred several years ago, probably with imported seed potatoes. The infested field was subjected to the phytosanitary measure of banning potato production for a period of 6 years with the possibility of its prolongation, if cysts with live content are found afterward. It is expected that the nematode is present in other fields due to the farmer's practices of propagating farm-saved seed potatoes and deficient field machinery hygiene. Therefore, the whole area will be intensively monitored for the presence of PCN in the future. An adequate pest management plan will be prepared after PCN pathotype identification. To our knowledge, only one field was found infested with G. pallida during the 3-year PCN survey in Bosnia and Herzegovina. Strict phytosanitary measures for preventing further PCN introductions and spreading should be intensified. References: (1) I. Ostojić et al. Plant Dis. 95:883, 2011. (2) S. Širca et al. Phytopathol. Mediterr. 49:361, 2010. (3) A. M. Skantar et al. J. Nematol. 39:133, 2007. (4) J. van Bezooijen. Methods and Techniques for Nematology, Wageningen University, 2006.

scholarly journals Morphological characteristics and distribution of Globodera species in Slovenia

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 354-357
Author(s):  
G. Urek ◽  
S. Širca ◽  
V. Meglič
Keyword(s):  
Hot Spot ◽  
Morphological Characteristics ◽  
Globodera Rostochiensis ◽  
Cyst Nematode ◽  
Soil Samples ◽  
Globodera Pallida ◽  
Cyst Nematodes ◽  
First Time ◽  
Austrian Border ◽  
Central Slovenia

Surveillance on cyst nematodes in Slovenia started already in 1963. We have found Globodera rostochiensis for the first time in 1971 and for the second time in 1975 (two cysts). In 1999 cysts of yellow potato cyst nematode were found again. A small hot spot was detected along the Slovene Austrian border. Cysts of Globodera rostochiensis were extracted from samples taken in 1.6 hectare field. In 2001 we surveyed 194 fields with the surface of 468.29 ha. We discovered another hot spot of Globodera rostochiensis in the Central Slovenia. Cysts of Globodera achilleae are seldom discovered in Slovenia. During the period between 1980 and 2000 we extracted altogether 206 cysts. In 2001 we found three fields where we extracted at first seven cysts. With more detailed survey we extracted another 30 cysts. In a few soil samples taken from the imported potatoes from Italy we have intercepted cysts of Globodera pallida. For that reason shipments were returned. In a similar way in 2002 shipments from Croatia were returned because of G. rostochiensis presence. Specimens of Globodera rostochiensis and G. achilleae species found in Slovenia were morphometricaly handeled.

scholarly journals A Survey of Globodera Rostochiensis on Potato Crops in Himachal Pradesh; Occurrence and Detection.

2021 ◽  
Author(s):  
Ashvika Pathania ◽  
Joydeep Dutta ◽  
Shashank Garg
Keyword(s):  
Population Density ◽  
Standard Procedure ◽  
Globodera Rostochiensis ◽  
Cyst Nematode ◽  
Himachal Pradesh ◽  
Soil Samples ◽  
Species Level ◽  
Molecular Techniques ◽  
Species Dominance ◽  
Potato Crops

Abstract A survey was carried out to estimate the frequency and occurrence of potato cyst nematode (PCN) in potato cultivating fields in different districts of Himachal Pradesh. A total of 109 composite soil samples were collected from 5 districts of Himachal Pradesh, India. Nematode cysts were extracted from the soil using standard procedure. The population density and percentage of occurrence of cyst nematode were calculated. According to the results district, Mandi had the highest PCN cyst population density, and the percentage of occurrence is 100%. District Kangra had the least PCN cyst population density and the percentage of occurrence is 66.6%. This survey showed the occurrence Globodera rostochiensis district-wise. The collected cysts were identified by morphological and molecular techniques, up to species level based on nucleotide homology and phylogenetic analysis. According to morphometrics distance between the vulva and anus is very less (65.69 ±22.2) µm. The outcome sequences are showed 95-99.05% similarity with sequence I.D KJ636272.1 from the Netherlands. This survey indicates that PCN Globodera rostochiensis were widely distributed in the potato cultivating fields of Himachal Pradesh. The present study helped determine the distribution of PCN in the soil and species dominance in particular areas. Hence, it helps design different strategies against PCN management.

scholarly journals Quarantine nematode species and pathotypes potentially dangerous for domestic potato production: populations diversity and the genetics of potato resistance

2020 ◽  
Vol 24 (7) ◽  
pp. 705-721
Author(s):  
N. V. Mironenko ◽  
T. A. Gavrilenko ◽  
A. V. Khiutti ◽  
O. S. Afanasenko
Keyword(s):  
Molecular Markers ◽  
Genetic Variability ◽  
Functional Organization ◽  
Potato Production ◽  
Potato Cultivars ◽  
Globodera Pallida ◽  
Parasitic Nematodes ◽  
Cyst Nematodes ◽  
Resistant Cultivars ◽  
Potato Resistance

The review considers quarantine species and nematode pathotypes potentially dangerous for domestic potato production. Potatoes are affected by more than 30 types of parasitic nematodes, but the review focuses on the most harmful representatives of genera that cause great damage to potato production: Globodera, Ditylenchus, Nacob bus and Meloidogyne. Phytopathological and molecular methods of identification of species and pathotypes and the main achievements in studying the population variability of parasitic potato nematodes were analyzed. It was shown that due to the peculiarities of the life cycle of nematodes and lability of their genomes, the genetic variability of these organisms is very high, which creates a threat of forming new pathogenic genotypes of the parasites. The information about the intra- and interpopulation variability of nematodes is important for studying the ways of introduction and distribution of separate species, as well as for searching for the correlations of molecular markers with the pathotype. Phylogenetic studies based on modern data on genetic variability of populations have allowed to reveal species complexes in Globodera pallida (Stone) Behrens and Nacobbus aberrans (Thorne) Thorne & Allen (sensu lato), including cryptic species. The main components of successful protection preventing a wide distribution of parasitic nematodes are quarantine measures, agricultural techniques, biological methods of protection and cultivation of resistant cultivars. Special attention in the review is paid to the breeding of potato cultivars with durable resistance to various nematode pathotypes, because the cultivation of such varieties is the most ecologically safe and economically advantageous way to prevent epiphytoties. Currently, significant progress has been made in the genetic protection of potato cultivars, especially against cyst-forming nematodes. The review provides data on sources of potato resistance to parasitic nematodes identified in collections of wild and cultivated species. Data on identified R-gens and QTL of resistance that have been introduced into breeding varieties using different methods and approaches are analyzed. The literature data on the study of structural and functional organization of genes for resistance to potato cyst nematodes are given. The results of molecular research on revealing the polymorphisms of loci involved in the control of resistance to cyst and gall nematodes, the development of molecular markers of certain genes and their use in marker-assisted selection for developing of new resistant cultivars, including those with group resistance, are considered.

scholarly journals Effect of Colletotrichum coccodes on Potato Yield, Tuber Quality, and Stem Colonization During Spring and Autumn

Plant Disease ◽  
1999 ◽  
Vol 83 (6) ◽  
pp. 561-565 ◽  
Author(s):  
Leah Tsror (Lahkim) ◽  
Orly Erlich ◽  
Marina Hazanovsky
Keyword(s):  
Vascular Tissue ◽  
Potato Yield ◽  
Potato Cultivars ◽  
Colletotrichum Coccodes ◽  
Tuber Quality ◽  
Yield Losses ◽  
Inoculation Methods ◽  
Significant Yield ◽  
Potato Crops

The response of five potato cultivars to Colletotrichum coccodes was tested in artificially inoculated fields for three consecutive spring and autumn seasons during 1994 to 1996. Significant yield reductions (22 to 30%) were observed in all tested cultivars. Results varied between years, but yield losses were more severe in autumn than in spring. Stem infections of plants were observed 90 days after planting on the surface of the stem and in vascular tissue. C. coccodes inoculation also resulted in reduction of the quality of daughter tubers. Cultivars Cara and Nicola were found to be less susceptible to tuber infection than Alpha, Desiree, and Agria. The incidence of diseased daughter tubers was higher when the soil was infested than when the foliage was inoculated. C. coccodes contamination of dry stems at harvest (in inoculated plots) was relatively high in all cultivars, with no difference between inoculation methods. Thus, C. coccodes infection not only affects potato yield and the quality of potatoes for seed and consumption, but also contaminates soil and serves as an important source of inoculum for future potato crops.

THE COMPETITIVENESS OF POLISH AND FOREIGN VARIETIES IN POTATOES PRODUCTION IN POLAND

2017 ◽  
Vol XIX (4) ◽  
pp. 141-147 ◽  
Author(s):  
Wojciech Nowacki
Keyword(s):  
Food Processing ◽  
Certified Seed ◽  
Potato Production ◽  
Potato Cultivars ◽  
Utility Value ◽  
Seed Potatoes ◽  
The Look ◽  
Starch Potato

The aim of this article is to assess the competitiveness of domestic and foreign potato cultivars grown in the country and to analyze the use of certified seed potatoes by the producers. The agronomic and utility value indicators were adopted as the measure of competitiveness of varieties. It has been shown that table cultivars of polish breeding in relation to foreign cultivars have generally higher agrotechnical value and higher yields but at the same time in many characteristics they have lower utility value (for example the look of tubers). The rate of use of certified seed potatoes depends on the degree of marketability of particular potato production sectors. It ranges from just 6.4% in multidirectional production to 25% in table and starch potato production, up to 40% in the production of early table potatoes and for food processing.

scholarly journals Evaluation of Potato Cultivars and Breeding Lines for Resistance to Globodera Rostochiensis and Globodera Pallida

2017 ◽  
Vol 76 (1) ◽  
pp. 3-8
Author(s):  
Anna Przetakiewicz ◽  
Dorota Milczarek
Keyword(s):  
Potato Cultivar ◽  
Nematode Resistance ◽  
Globodera Rostochiensis ◽  
Potato Cultivars ◽  
Globodera Pallida ◽  
Assessment Procedure ◽  
Breeding Lines ◽  
Alternative Approach ◽  
Biological Tests ◽  
Significant Yield

Abstract Nematodes are among the most important agents affecting potato crops. Heavy infestations by Globodera rostochiensis and G. pallida can cause significant yield losses and limit the choice of potato cultivars that can be grown effectively (Oerke, 2006). Breeding of new potato cultivar resistant to G. rostochiensis and G. pallida is a long-term process. It is preceded by screening of potato breeding lines for resistance in repeated biotests, which seems to be the most effective and safest method of protection. Evaluation of nematode resistance is an important element of characterization of breeding lines and new cultivars. Resistance to Globodera spp. is evaluated in biological tests. The use of DNA markers for detecting nematode resistance genes may be an alternative approach to phenotypic evaluation of resistance degree of potato plants (Jena and Mackill, 2008). The goal of this report is focused on a description of resistance assessment procedure of breeding lines and varieties of potato to PCN and on comparison of biological and molecular methods of resistance evaluation. Presented information is addressed to both breeders and Polish inspection services.

INTERNATIONAL GRANT COOPERATION AS A TOOL FOR THE DEVELOPMENT OF PROFESSIONAL COMPETENCIES OF UNIVERSITY TEACHERS

2018 ◽  
pp. 4-8
Author(s):  
Milan Chmura
Keyword(s):  
Higher Education ◽  
Czech Republic ◽  
Professional Education ◽  
European Countries ◽  
International Project ◽  
The Czech Republic ◽  
University Teachers ◽  
Quality Of Higher Education ◽  
Education And Development

The education and development of university teachers have its justifcation and its importance is signifcant not only in the Czech Republic but also abroad. This study provides an analysis of further professional education of university teachers in the Czech Republic and in selected European countries. Subsequently, it presents an international project with participants from the Czech Republic, Ukraine, Slovakia and Poland, which, ultimately, plays a role in the improvement of the quality of higher education.

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