scholarly journals Synthesis of a novel aminobenzene-containing hemicucurbituril and its fluorescence spectral properties with ions

2021 ◽  
Vol 17 ◽  
pp. 2840-2847
Author(s):  
Qingkai Zeng ◽  
Qiumeng Long ◽  
Jihong Lu ◽  
Li Wang ◽  
Yuting You ◽  
...  
Keyword(s):  
Experimental Evidence ◽  
Fluorescence Intensity ◽  
Spectral Properties ◽  
Association Constant ◽  
Fluorescence Spectra ◽  
Metal Cations ◽  
Fluorescent Chemosensor ◽  
Strong Interactions ◽  
Alkaline Environment ◽  
Coupling Strategy

A novel hemicucurbituril-based macrocycle, alternately consisting of amidobenzene and 2-imidazolidione moieties was designed and synthesized. Based on the fragment coupling strategy, nitrobenzene-containing hemicucurbituril was firstly prepared facilely under alkaline environment, and reduction of the nitro groups produced the desired amidobenzene-containing hemicucurbituril. As an original fluorescent chemosensor, it exhibited strong interactions with Fe3+ over other metal cations. The experimental evidence of fluorescence spectra suggested that a 1:1 complex was formed between this macrocycle and Fe3+ with an association constant up to (2.1 ± 0.3) × 104 M−1. Meanwhile, this macrocycle showed no obvious or only slight enhancement of the fluorescence intensity with selected anions.

A phthalocyanine-based fluorescent sensor for Zn2+ ion

2013 ◽  
Vol 17 (01n02) ◽  
pp. 99-103 ◽  
Author(s):  
Hui He ◽  
Jian-Yong Liu ◽  
Dennis K.P. Ng
Keyword(s):  
Metal Ions ◽  
Spectral Properties ◽  
Fluorescence Spectra ◽  
Near Infrared ◽  
Fluorescent Sensor ◽  
High Sensitivity ◽  
Absorption And Fluorescence Spectra

This paper describes the preparation and spectral properties of a near-infrared fluorophore in which two bis(2-picolyl)amino moieties are axially linked to a silicon(IV) phthalocyanine core. The effects of various metal ions on its absorption and fluorescence spectra have been examined. The results indicate that this compound shows a high sensitivity and moderate selectivity toward Zn2+ ion.

scholarly journals Competitive Binding of Novel Cyanine Dye AK3-5 and Europium Coordination Complexes to DNA

2019 ◽  
Keyword(s):  
Dna Binding ◽  
Fluorescence Intensity ◽  
Binding Sites ◽  
Association Constant ◽  
Antitumor Agents ◽  
Coordination Complexes ◽  
Cyanine Dye ◽  
Europium Complexes ◽  
Intensity Decrease ◽  
Dna Binding Sites

The present study was undertaken to assess the applicability of the novel trimethine cyanine dye AK3-5 as a competitive ligand for the antitumor agents, Eu(III) coordination complexes (EC), in the DNA-containing systems, using the displacement assay as an analytical instrument. The analysis of fluorescence spectra revealed a strong association of AK3-5 with nucleic acids, with the strength of interaction being higher for the double stranded DNA, compared to the single-stranded RNA. The binding parameters of the cyanine dye have been determined in terms of the McGhee & von Hippel neighbouring site-exclusion model and a classical Langmuir model. The AK3-5 association constant in the presence of DNA was found to be equal to 5.1×104 M-1, which is consistent to those of the well-known DNA intercalators. In turn, the binding of the cyanine to the RNA was characterized by a significantly lower association constant ( ~ 3.4×103 M-1) indicating either the external or “partially intercalated” binding mode. The addition of the europium complexes to the AK3-5-DNA system was followed by the fluorescence intensity decrease, with a magnitude of this effect being dependent on the EC structure. The observed fluorescence decrease of AK3-5 in the presence of europium complexes V7 and V9 points to the competition between the cyanine dye and antitumor drugs for the DNA binding sites. The dependencies of the AK3-5-DNA fluorescence intensity decrease vs. europium complex concentration were analyzed in terms of the Langmuir adsorption model, giving the values of the drug association constant equal to 5.4×104 M-1and 3.9×105 M-1 for the europium complexes V7 and V9, respectively. A more pronounced decrease of the AK3-5 fluorescence in the presence of V5 and V10 was interpreted in terms of the drug-induced quenching of the dye fluorescence, accompanying the competition between AK3-5 and Eu(III) complexes for the DNA binding sites. Cumulatively, the results presented here strongly suggest that AK3-5 can be effectively used in the nucleic acid studies and in the dye-drug displacement assays.

Quantum Dots CdSe/ZnS-Loaded Poly(D,L-Lactide-Co-Glycolide) Nanoparticles: Physicochemical Characterization and Application

2006 ◽  
Vol 505-507 ◽  
pp. 667-672 ◽  
Author(s):  
Chih Hui Yang ◽  
Kuo Chin Lin ◽  
Yu Huai Chang ◽  
Yu Cheng Lin
Keyword(s):  
Quantum Dots ◽  
Fluorescence Intensity ◽  
Fluorescence Spectra ◽  
Uv Light ◽  
Physicochemical Characterization ◽  
Plga Nanoparticles ◽  
Optimum Concentration ◽  
Cellular Internalization ◽  
Optimum Concentration Range

This paper described and characterized the quantum dots (QDs) with/without the polymeric PLGA applied in MC3T3E-1 delivery. Neat QDs were treated with various solvents, temperatures, exposure time and concentration to evaluate their stability and efficacy. We found that the intensity degree of fluorescence spectra (QDs) in different solvents follows the order: ether > THF > acetone > chloroform > methanol. Importantly, the QDs become inactive after 8-hr dissolution in the solvents of ether, THF or chloroform. According to this result, acetone and methanol are ideal solvents for QDs. The optimum concentration range of QDs in acetone is 5 to 10 mg/mL. We found that no obvious difference of fluorescence intensity was detected in QDs stored respectively at 4 °C, 24 °C and 44 °C (8-hour). When QDs were exposed to UV light (312 nm) for 2 hr, serious decay of fluorescence intensity was observed. In order to extend the application of QDs in medical areas, we encapsulated them in individual biocompatible poly(d,l-lactide-co-glycolide) (PLGA) nanoparticles for in-vitro imaging of endocytosis in MC3T3E-1 cells. We demonstrated that the polymeric PLGA have the ability to permeate the cells for cellular internalization; the endocytotic activity could be enhanced by the polymeric QDs-encapsulated PLGA.

scholarly journals Establishment of Fluorescence Sensitization Method for Hydroxysafflor Yellow A

2020 ◽  
Vol 2020 ◽  
pp. 1-13 ◽  
Author(s):  
CAO Haiyan ◽  
QIN Xiude ◽  
LIU Chen ◽  
ZHAO Xinzhe ◽  
MA Yuhui ◽  
...  
Keyword(s):  
Detection Limit ◽  
Fluorescence Intensity ◽  
Fluorescence Spectra ◽  
Pharmacological Effects ◽  
Absorption And Fluorescence Spectra ◽  
Hydroxysafflor Yellow A ◽  
T24 Cells ◽  
Fluorescence Sensitization ◽  
In Cells

As the main active ingredient in Chinese medicine safflower, hydroxysafflor yellow A (HSYA) has multiple pharmacological effects. In the work, the absorption and fluorescence spectra of HSYA under different environmental conditions (such as acidity, temperature, ions, viscosity, and surfactant) were investigated. The fluorescence intensity of HSYA varied greatly with acidity, temperature, viscosity, and surfactant, but was less affected by common cations and anions. Among various surfactants, we found that borax can significantly enhance the HSYA fluorescence intensity, and thus, a borax-HSYA sensitization system for HSYA fluorescence was established. In the optimized sensitization system, the fluorescence intensity of HSYA increased by 20 times and showed a good linearity with HSYA concentrations in the range of 0∼10 μM with a detection limit of 8 nM. The borax-HSYA sensitization system is nontoxic to T24 cells and mice and can be used for the fluorescence imaging of HSYA in cells, thereby providing an effective method for analyzing HSYA in vitro and monitoring its metabolism in cells.

Study on Three-Dimensional Fluorescence Spectra of Oil Concentration in Water

2011 ◽  
Vol 322 ◽  
pp. 144-147
Author(s):  
Yu Tian Wang ◽  
Li Li Yang ◽  
Xin Qiong Lu
Keyword(s):  
Quantitative Analysis ◽  
Fluorescence Intensity ◽  
Fluorescence Spectra ◽  
Three Dimensional ◽  
Measurement Model ◽  
Excitation Wavelength ◽  
Experimental Basis ◽  
Oil In Water ◽  
Oil Concentration ◽  
Fluorescence Spectrometer

According to the Lambert-Beer laws, the fluorescence spectra of oil in water were investigated by measuring excitation-emission matrixes with FS920 fluorescence spectrometer. On the basis of the three-dimensional fluorescence spectra of oil in water, the excitation wavelength of 290nm and emission wavelength of 324nm were chosen for the quantitative analysis of oil in water. The results show that the concentration of oil in water is linearly proportional to the fluorescence intensity in the range of 1ppm-100ppm, and the measurement model of concentration is obtained with the linear correlation coefficient of 0.9993. The artificial samples has also been tested and the results are satisfactory. The recovery is in the range of 92%-106%. The research provides experimental basis for the quantitative analysis of multi-component oil in water.

scholarly journals Experimental evidence of TICT state in 4-piperidinyl-1,8-naphthalimide – a kinetic and mechanistic study

2018 ◽  
Vol 20 (15) ◽  
pp. 10155-10164 ◽  
Author(s):  
Zoltán Szakács ◽  
Sylvia Rousseva ◽  
Márton Bojtár ◽  
Dóra Hessz ◽  
István Bitter ◽  
...  
Keyword(s):  
Excited State ◽  
Experimental Evidence ◽  
Fluorescence Spectra ◽  
Mechanistic Study

The excited state processes in N-propyl-4-piperidinyl-1,8-naphthalimide have been studied by measuring its fluorescence spectra and decay curves in solvents of different polarity and viscosity and also in a frozen solvent glass.

Inner Filter Effect on Fluorescence Dyes Spectra in Methanol Solution

2016 ◽  
Vol 675-676 ◽  
pp. 704-707 ◽  
Author(s):  
Saksit Sukprasong ◽  
Yongyut Manjit ◽  
Apichart Limpichaipanit ◽  
Athipong Ngamjarurojana
Keyword(s):  
Fluorescence Intensity ◽  
Fluorescence Spectra ◽  
Inner Filter Effect ◽  
Fluorescent Dye ◽  
Concentration Effect ◽  
Wavelength Shift ◽  
Filter Effect ◽  
High Concentrations ◽  
Fluorescence Dyes ◽  
Dye Solutions

This research was conducted to study the inner filter effect on the fluorescence spectra of fluorescence dyes. The concentration effect on all of fluorescent dye solutions showed the same trend in terms of changes in fluorescence intensity spectra. At low concentrations, the fluorescence intensity increased when the concentration of fluorescent dye solution increased. However, at high concentrations, the fluorescence intensity decreased when the concentration of fluorescent dye solution increased. Interestingly, the result of fluorescence spectra in dye solutions showed that the fluorescence intensity maxima in all dye solutions were shifted to a higher wavelength (red-shift) when the concentration of fluorescent dye solution increased. The results of concentration effect on fluorescence intensity and wavelength-shift in dyes solution can be explained by inner filter effect on fluorescent dye solutions.

Design and synthesis of a novel rhodamine-based chemosensor and recognition study to Fe3+

2016 ◽  
Vol 22 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Feifei Zhang ◽  
Qi Gao ◽  
Junxia Zhao ◽  
Man Ge ◽  
Yinjuan Bai
Keyword(s):  
Fluorescence Intensity ◽  
Rhodamine B ◽  
Association Constant ◽  
Color Change ◽  
Condensation Reaction ◽  
Crystal Data ◽  
Optimum Conditions ◽  
Design And Synthesis ◽  
Colorimetric Chemosensor ◽  
Binding Stoichiometry

AbstractA fluorescent and colorimetric chemosensor Rh1 for Fe3+ was synthesized by condensation reaction of rhodamine B hydrochloride and 2-aminothiazole, and its structure was confirmed by NMR, IR, HRMS and crystal data. Upon coordination with Fe3+ in CH3CN-H2O (1:1, v/v) solution, the spirolactam of Rh1 is opened, which results in a dramatic enhancement of fluorescence intensity as well as the color change of the solution. Most importantly, other metal ions show no obvious interference with the detection of Fe3+. Under the optimum conditions described, the fluorescence intensity is linearly proportional to the concentration of Fe3+ in the range of 2 μm ~ 7 μm. The Job’s plot indicates a 1:1 binding stoichiometry between Rh1 and Fe3+. The association constant (Ka) is 2.26 × 104m-1.

scholarly journals Improved spectrofluorimetric methods for determination of penicillamine in capsules

2010 ◽  
Vol 8 (4) ◽  
pp. 892-898 ◽  
Author(s):  
Rasha Shaalan
Keyword(s):  
Acidic Medium ◽  
Spectral Properties ◽  
Fluorescence Spectra ◽  
Reaction Conditions ◽  
Bulk Powder ◽  
Compound Method ◽  
Subsequent Measurement ◽  
Medium Method ◽  
Good Agreement

AbstractTwo simple, sensitive and specific fluorimetric methods have been developed for the determination of Penicillamine (PNC), a sulphur containing compound. Method (I) involves the reaction of PNC with 2′,7′-bis(acetoxymercuri)-fluorescein (AMF) in the presence of Kolthoff’s buffer, pH 8.2, with subsequent measurement of fluorescence spectra at 520 nm (λEx 497 nm). Method (II) is based on PNC being oxidized into penicillaminic acid using Cerium (IV) in an acidic medium. Method sensitivity has been improved using sodium triphosphate which enhances the luminescence intensity of Ce(III). Fluorescence spectra were then measured at 348 nm (λEx 293 nm). The reaction conditions and the fluorescence spectral properties have been investigated for both methods. Under the described conditions, the proposed methods were applicable over the concentration ranges 0.0048 − 0.0288 µg mL−1 and 0.096 − 0.288 µg mL−1 with mean percentage recoveries 99.95 ± 1.29 and 100.04 ± 1.10 for methods I and II, respectively. The proposed methods were validated in terms of accuracy, precision, LOD and LOQ and robustness and then were successfully applied to the determination of PNC in bulk powder and in capsules as well as in the presence of the related disulphide. The results obtained were determined to be in good agreement with those obtained using a previously reported method.

Research on Photochromic Materials with Synthesis and Application of 1-(2-methyl-3-benzothienyl)-2-[2-methyl-(5-ethynyl)trimethylsilane-3-thienyl] Perfluorocyclopentene

2014 ◽  
Vol 1003 ◽  
pp. 35-38
Author(s):  
Le Le Ma ◽  
Hong Yan Xu ◽  
Gang Liu
Keyword(s):  
Fluorescence Intensity ◽  
Fluorescence Spectra ◽  
Uv Light ◽  
Optoelectronic Properties ◽  
Closed Ring ◽  
Open Ring ◽  
Photochromic Materials ◽  
Hexane Solution

A new unsymmetrical photochromic diarylethene compound, 1-(2-methyl-3-benzothienyl)-2-(2-methyl-(5-ethynyl) trimethylsilane-3-thienyl) perfluorocyclopentene (1o) has been synthesized, and its optoelectronic properties, such as photochromism and the fluorescence spectra of diarylethene 1o in hexane solution was investigated. The results showed that this compound exhibited reversible photochromism in solution. The maxima absorption of compound closed-ring isomer 1c are 538 nm. Its fluorescence intensity decreased along with the photochromism from open-ring isomers to closed-ring isomers upon irradiation with 297 nm UV light.

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