Copper nanoparticles together with cold atmospheric plasma improves the growth and physiological indices of Dracocephalum moldavica in hydroponic system

2018 ◽  
Vol 7 (3) ◽  
pp. 130-139
Author(s):  
Kazhal Haddadian ◽  
Alireza Iranbakhsh ◽  
Ramazan Ali Khavari-Nejad ◽  
Mahmood Ghoranneviss
Keyword(s):  
Copper Nanoparticles ◽  
Dry Weight ◽  
Culture Conditions ◽  
Atmospheric Plasma ◽  
Shoot Height ◽  
Cold Atmospheric Plasma ◽  
Shoot Dry Weight ◽  
Physiological Indices ◽  
Soluble Leaf Protein ◽  
Herbal Plant

This experiment was conducted to investigate the effect of copper nanoparticles and cold atmospheric plasma on growth and physiological indices of Dracocephalum moldavica herbal plant under hydroponic culture conditions. The factors investigated in this experiment were copper nanoparticles at four levels of zero (control), 25, 50, and 75 ppm and cold atmospheric plas-ma at three times of zero (control), 20, and 30 seconds. The results showed that application of cold atmospheric plasma led to an increase in the shoot height, root length, shoot dry weight, root dry weight, leaf area, chlorophyll a, chlorophyll b, total chlorophyll, soluble leaf protein; it also decreased proline content. In addition, there was no significant difference between cold atmospheric plasma at the two durations of 20 and 30 seconds. Moreover, compared with the control and non-nanoparticle copper treatments, the use of copper nanoparticles improved the measured indices; accordingly, when making its greatest effect, the use of copper nanoparticles led to 27.77% increase in shoot height, 73.76% in root length, 67.98% in shoot dry weight, 87.67% in root dry weight, 22.83% in leaf area, 76.19% in soluble leaf protein in treatment with 25 ppm of copper and 39.42%, 50%, and 42.36% increase in chlorophyll a, chlorophyll b, total chlorophyll, respectively in treatment with 50 ppm of copper nanoparticles. Considering the obtained results, it can be concluded that the use of 25 ppm of copper nanoparticles (due to the application of nanoparticle instead of non-nanoparticle copper) within 20 seconds of cold atmospheric plasma improves the growth and physiological indices of D. moldavica herbal plant under hydroponic culture conditions.

scholarly journals AVOCADO SEEDLINGS MULTIPLE STEMS PRODUCTION

2016 ◽  
Vol 38 (2) ◽  
Author(s):  
MARCUS VINICIUS SANDOVAL PAIXÃO ◽  
JOSÉ CARLOS LOPES ◽  
EDILSON ROMAIS SCHMILDT ◽  
RODRIGO SOBREIRA ALEXANDRE ◽  
CAROLINE MERLO MENEGHELLI
Keyword(s):  
Root Length ◽  
Dry Weight ◽  
Adventitious Rooting ◽  
Dry Mass ◽  
Stem Diameter ◽  
Experimental Unit ◽  
Shoot Height ◽  
Shoot Dry Weight ◽  
Federal Institute ◽  
Multiple Stems

ABSTRACT This study aimed to evaluate the potential of multi-stems in avocado seeds according to their mass as well as the adventitious rooting of multi-stem budding with or without the use of auxin. The research was carried out at the Vegetation House of Federal Institute of Espírito Santo, Campus Santa Teresa -ES, with seeds of different masses: <60 g, 61 to 80 g, 81 to 100 g and >100 g, in which each experimental unit was made of five seeds, distributed within five repetitions, under a completely randomized design. The seeds were put to germinate and the percentage number of emergence and multiple stems were evaluated. After 150 days, the following evaluations were carried out: survival of rooted cuttings; number of leaves; stem diameter; root length; root volume; root and shoot fresh mass; root and shoot dry mass; shoot height; absolute growth and shoot growth rate; shoot dry weight/root dry mass ratio; shoot height/stem diameter ratio; shoot height/root length and Dickson's quality index ratio. Avocado seeds with mass over 100 g and between 81-100 g presented higher percentage of multiple stems. Rods over 20 cm that were not treated with IBA (indole-3-butyric acid) resulted on avocado plants of better quality. The use of IBA (2000 mg L-1) does not affect the rooting and growth of avocado's multi-stem plants.

scholarly journals Reproducibility of suppression of Pythium wilt of cucumber by compost

2014 ◽  
Vol 23 (3) ◽  
pp. 236-245 ◽  
Author(s):  
Mauritz Vilhelm Vestberg ◽  
Sanna Kukkonen ◽  
Päivi Parikka ◽  
Dan Yu ◽  
Martin Romantschuk
Keyword(s):  
Raw Materials ◽  
Dry Weight ◽  
Shoot Height ◽  
Bacterial Diseases ◽  
Sphagnum Peat ◽  
Shoot Dry Weight ◽  
Suppressive Capacity ◽  
The Difference ◽  
Set Up

There is increasing global interest in using compost to suppress soil-borne fungal and bacterial diseases and nematodes. We studied the reproducibility of compost suppressive capacity (SC) against Pythium wilt of cucumber using nine composts produced by the same composting plant in 2008 and 2009. A bioassay was set up in a greenhouse using cucumber inoculated with two strains of Pythium. The composts were used as 20% mixtures (v:v) of a basic steam-sterilized light Sphagnum peat and sand (3:1, v:v). Shoot height was measured weekly during the 5-week experiment. At harvest, the SC was calculated as the % difference in shoot dry weight (DW) between non-inoculated and inoculated cucumbers. The SC was not affected by year of production (2008 or 2009), indicating reproducibility of SC when the raw materials and the composting method are not changed. Differences in shoot height were not as pronounced as those for shoot DW. The results were encouraging, but further studies are still needed for producing compost with guaranteed suppressiveness properties.

scholarly journals Emergence and Shoot Growth of Cosmos and Marigold from Paclobutrazol-treated Seed

2001 ◽  
Vol 19 (1) ◽  
pp. 11-14 ◽  
Author(s):  
Wallace G. Pill ◽  
James A. Gunter
Keyword(s):  
Growth Medium ◽  
Shoot Growth ◽  
Dry Weight ◽  
Tagetes Patula ◽  
Shoot Height ◽  
Shoot Dry Weight ◽  
Grade 5 ◽  
Treated Seed ◽  
Emergence Percentage ◽  
And Control

Abstract This study was conducted to determine whether treating seeds of ‘Sensation Mixed’ cosmos (Cosmos bipinnatus Cav.) and ‘Bonanza Gold’ marigold (Tagetes patula L.) with paclobutrazol (PB) could suppress seedling growth. Seeds were soaked in solutions of 0, 500 or 1000 mg PB/liter (ppm PB) for 16 hours at 25C (77F) or they were primed [−0.5 MPa (−5 bars) for 7 days at 20C (68F)] in Grade 5 exfoliated vermiculite moistened with 0, 500 or 1000 ppm PB solltuions. Soaked and primed seeds were dried for 1 day at 19C (65F) and 25% relative humidity. These seeds and control (non-treated) seeds were sown into plug cells containing peat-lite. Increasing PB concentration decreased cosmos shoot height at 32 days after planting (DAP), but decreased emergence percentage, responses that were more pronounced with priming than with soaking. A 1 ppm PB growth medium drench [30 ml/cell(0.2 mg PB/cell)] and, to a greater extent a 10 mg PB/liter (ppm PB) shoot spray [2 ml/shoot (0.02 mg PB/shoot)], both applied at 10 DAP, resulted in greater cosmos shoot height suppression at 32 DAP than treatment of seeds with 1000 ppm PB. Soaking marigold seeds in 1000 ppm PB failed to decrease shoot height below those of plants from non-treated seeds at 32 DAP. However, exposure to 1000 ppm PB during priming of marigold seeds resulted in a similar shoot height suppression (13%) as the growth medium drench, and similar shoot dry weight reduction (21%) as the shoot spray. Suppression of shoot growth by this seed treatment was short-term since by five weeks after transplanting into 15 cm (6 in) pots, only marigold plants that had received the growth medium drench or shoot spray were smaller than those of control plants. Treating marigold seeds with 1000 mg ppm PB used about one-fifth the PB used to drench the growth medium.

Effect of Herbicides on Germination and Growth of Four Grass Weeds

Weed Science ◽  
1984 ◽  
Vol 32 (4) ◽  
pp. 489-493 ◽  
Author(s):  
Frank L. Young ◽  
David R. Gealy ◽  
Larry A. Morrow
Keyword(s):  
Bromus Tectorum ◽  
Soil Surface ◽  
Dry Weight ◽  
Shoot Elongation ◽  
Downy Brome ◽  
Aegilops Cylindrica ◽  
Shoot Height ◽  
Shoot Dry Weight ◽  
Jointed Goatgrass ◽  
Germination And Growth

In the greenhouse, glyphosate [N-(phosphonomethyl)glycine] at 0.6 kg ae/ha applied directly to seeds alone or seeds on the soil surface reduced germination and shoot dry weight of common rye (Secale cerealeL. ♯3SECCE). Paraquat (1,1′-dimethyl-4,4′-bipyridinium ion) applied similarly at 0.6 kg ai/ha reduced germination and shoot dry weight of downy brome (Bromus tectorumL. ♯ BROTE) and wheat (Triticum aestivumL. ‘Daws' ♯ TRZAX). Metribuzin [4-amino-6-tert-butyl-3-(methylthio)-as-triazin-5 (4H)-one] at 0.6 kg ai/ha applied to seeds, soil, or seeds and soil had very little effect on germination, but significantly reduced shoot dry weight of common rye, downy brome, wheat, and jointed goatgrass (Aegilops cylindricaHost. ♯ AEGCY). Pronamide [3,5-dichloro(N-1,1-dimethyl-2-propynyl)benzamide] at 0.6 kg ai/ha, and propham (isopropyl carbanilate) at 3.4 kg ai/ha plus extender (p-chlorophenyl-N-methylcarbamate) at 0.4 kg ai/ha substantially reduced shoot height and dry weight of all species, regardless of application method, with pronamide completely inhibiting shoot elongation and dry-weight production in three of the four species.

scholarly journals The Impact of Nickel Mining on Soil Properties and Growth of Two Fast-Growing Tropical Trees Species

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Ricksy Prematuri ◽  
Maman Turjaman ◽  
Takumi Sato ◽  
Keitaro Tawaraya
Keyword(s):  
Forest Soil ◽  
Dry Weight ◽  
Natural Forest ◽  
Total Carbon ◽  
Available Phosphorus ◽  
Shoot Height ◽  
Fast Growing ◽  
Shoot Dry Weight ◽  
Nickel Mining ◽  
The Impact

Opencast nickel mining is common in natural forests of Indonesia. However, rehabilitation of postmining degraded land is difficult. We investigated the effect of opencast nickel mining on soil chemical properties and the growth of two fast-growing tropical tree species, Falcataria moluccana and Albizia saman. Soil was collected from post-nickel mining land and a nearby natural forest. Soil pH, available phosphorus (P) concentration, total carbon (TC) and total nitrogen (TN) concentration, C/N ratio, cation exchange capacity (CEC), and exchangeable K, Na, Mg, Ca, Fe, and Ni concentrations were determined. Falcataria moluccana and A. saman were then grown in the collected soils for 15 weeks in a greenhouse. Shoot height and shoot and root dry weights of the seedlings were measured. The post--nickel mining soils TN, TC, available P, CEC, and exchangeable Ca and Na concentrations decreased by 98%, 93%, 11%, 62%, 85%, and 74%, respectively, in comparison with the natural forest soils. The pH of postmining soil was higher than natural forest soil. Shoot dry weight of F. moluccana seedlings grown in postmining soil was significantly ( P < 0.05 ) lower than that of seedlings grown in natural forest soil. However, there was no difference in shoot dry weight between A. saman seedlings grown in natural forest soil and postmining soil, as well as root dry weights of both species. The results indicate that opencast nickel mining decreased soil fertility, which subsequently inhibited the growth of F. moluccana and A. saman seedlings.

scholarly journals Influence of culture vessel volume and ventilation on in vitro culture and adenosine accumulation of Anoectochilus setaceus Blume

2018 ◽  
Vol 15 (2) ◽  
pp. 307-317
Author(s):  
Vũ Quốc Luận ◽  
Nguyễn Bá Nam ◽  
Vũ Thị Hiền ◽  
Nguyễn Phúc Huy ◽  
Hoàng Thanh Tùng ◽  
...  
Keyword(s):  
Liquid Medium ◽  
Dry Weight ◽  
Culture Conditions ◽  
Culture Vessel ◽  
Shoot Height ◽  
Factors Affecting ◽  
Vessel Volume ◽  
Plastic Bag ◽  
Tlc Analysis

Anoectochilus setaceus is a medicinal plant used as a traditional medicine as well as nutritious products for health care in China and many Asian countries. In conventional propagation, the the vessel type, material and volume lead to differences in the moisture content between the internal and external conditions which result in great impact on the quality of in vitro plantlets as well as subsequent growth at nursery. In this study, several factors affecting the multiplication and regeneration of A. setaceus cultured in vitro were investigated. The highest percentage of shoot regeneration (52.88%) from ex vitro stem nodes was obtained when explants were cultured on SH medium supplemented with 1 mg/l BA, 30 g/l sucrose with cotton wool plug as the substrate and pH of the medium was adjusted to 5.8 prior to autoclaving. In the present study, culture vessel volume and ventilation culture conditions indicated to have effect on shoot quality. Results showed that 3-liter Erlenmeyer flasks in combination with ventilation caps resulted in better shoot growth and development (shoot height: 10.75 cm; 9.45 leaves per shoot; fresh- and dry- weight of 2.45 and 0.219 g per shoot, respectively; leaf diameter: 2.85 cm and SPAD: 39.99). Results of chromatography (TLC) analysis indicated that explants gave the same Rf value as that of authentic Adenosine. Qualifying Adenosine using TLC showed that extracts of shoots cultured on solid and liquid medium (ventilation plastic bag), and that of shoots cultured in 3-liter vessel with liquid medium (with and without ventilation) gave the same pattern in the respects of color and Rf value.

scholarly journals Response of Sophora secundiflora to Nitrogen Form and Rate

HortScience ◽  
2011 ◽  
Vol 46 (9) ◽  
pp. 1303-1307 ◽  
Author(s):  
Genhua Niu ◽  
Denise Rodriguez ◽  
Mengmeng Gu
Keyword(s):  
Dry Weight ◽  
Nitrogen Form ◽  
Shoot Height ◽  
Root Dry Weight ◽  
Shoot Dry Weight ◽  
Shoot Ratio ◽  
Significant Difference ◽  
Root To Shoot Ratio ◽  
Total Dry Weight ◽  
Mountain Laurel

Texas mountain laurel (Sophora secundiflora) is a native shrub tolerating drought, heat, windy conditions, and alkaline or wet soils. However, its availability is somewhat low and little information is available on nutrient requirement and other culture information. Two greenhouse experiments were conducted to quantify the responses of Texas mountain laurel to different forms and rates of nitrogen (N) fertilizer. In Expt. 1, 1-year old seedlings were treated for 194 days with three NO3:NH4 ratios at 25:75, 50:50, and 75:25 and two rates of N at 100 and 200 mg·L−1 in a factorial design. There was no interaction between the N rate and form on any growth parameters. Nitrogen form did not significantly affect shoot dry weight, root dry weight, root–to-shoot ratio, or the total dry weight. There was no significant difference between N rate of 100 and 200 mg·L−1 on root dry weight, root-to-shoot ratio, or the total dry weight. The shoot dry weight of Texas mountain laurel fertilized with 100 mg·L−1 was higher compared with that of the plants fertilized at 200 mg·L−1. The reduced shoot dry weight at N of 200 mg·L−1 was the result of the higher substrate salinity. In Expt. 2, seedlings were fertilized with five N rates (50, 100, 150, 200, and 250 mg·L−1) for 203 days. Plants watered with 150, 200, and 250 mg·L−1 were taller than those fertilized with 50 mg·L−1. The shoot height of plants watered with 100 mg·L−1 was only significantly different from 50 mg·L−1. For rapid growth of Texas mountain laurel, a N rate range of ≈150 mg·L−1 was recommended supplied with a combination of NO3-N and NH4-N in the ratios of 0.3 to 3.0.

scholarly journals Effects of Some Growth Retardants on Shoot and Root Growth of Two Cool-season Turfgrasses

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 895E-895 ◽  
Author(s):  
Fahed A. Al-Mana ◽  
Hesham H. Abdel-Kader ◽  
Ritchard J. Bisarove
Keyword(s):  
Root Growth ◽  
Perennial Ryegrass ◽  
Dry Weight ◽  
Major Effect ◽  
Cool Season ◽  
Shoot Height ◽  
Red Fescue ◽  
Shoot Dry Weight ◽  
Turf Quality ◽  
Shoot And Root Growth

Effects of mefluidide, paclobutrazol, and their mixture on shoot and root growth of perennial ryegrass (Lolium perenne L. `Wendy') and creeping red fescue (Festuca rubra L. `Dawson') were studied under container culture. Mefluidide applied alone or in combination with paclobutrazol caused significant reduction in shoot and root growth of perennial ryegrass and red fescue. These treatments also enhanced turf green color of both species and increased their root–shoot percentage, with no major effect on turf quality. Paclobutrazol applied alone reduced shoot height of perennial ryegrass and red fescue by 10% and 32%, respectively, and caused little reduction in their shoot weights, with no effect on turf quality and color. Although paclobutrazol applied alone reduced the root length and percentage of root–shoot dry weight of perennial ryegrass, it did not affect red fescue.

scholarly journals Sensitivity of tumor versus normal cell migration and morphology to cold atmospheric plasma‐treated media in varying culture conditions

2019 ◽  
Vol 17 (2) ◽  
pp. 1900103 ◽  
Author(s):  
Marina A. Pranda ◽  
Brittney J. Murugesan ◽  
Andrew J. Knoll ◽  
Gottlieb S. Oehrlein ◽  
Kimberly M. Stroka
Keyword(s):  
Cell Migration ◽  
Normal Cell ◽  
Culture Conditions ◽  
Atmospheric Plasma ◽  
Cold Atmospheric Plasma

scholarly journals Controlled-Release Fertiliser and Substrates on Seedling Growth and Quality in Agonandra brasiliensis in Roraima

2020 ◽  
Vol 8 (3) ◽  
pp. 70 ◽  
Author(s):  
Aline das Graças Souza ◽  
Oscar José Smiderle ◽  
Raiovane Araújo Montenegro ◽  
Thiago Komuro Moriyama ◽  
Thiago Jardelino Dias
Keyword(s):  
Controlled Release ◽  
High Standard ◽  
Dry Weight ◽  
Organic Substrate ◽  
Shoot Height ◽  
Shoot Dry Weight ◽  
Slow Development ◽  
Collar Diameter ◽  
Total Dry Weight

The aim of this study was to verify the effect of different substrates, in the presence and absence of controlled-release fertiliser, on the growth and morphological quality of seedlings of Agonandra brasiliensis Miers ex Benth. & Hook.f., in the state of Roraima, Brazil. The experimental design was a 2 * 5 factorial scheme, with and without the addition of 1.0 g L-1 NPK 18-05-09 formulation controlled-release encapsulated fertiliser (Forth CoteR) and five substrates. The following were evaluated: shoot height (H), collar diameter (CD), increase in shoot length (IncH) and collar diameter (IncCD), shoot dry weight (SDW), root dry weight (RDW), total dry weight (TDW) and Dickson quality index (DQI). The use of NPK 18-05-09 formulation controlled-release fertiliser (Forth CoteR) in a medium-sand substrate is recommended for obtaining plants of Agonandra brasiliensis of greater robustness, balance of biomass distribution, and a high standard of quality. In the substrate composed of soil from the cerrado + carbonised rice husks - CRH + organic substrate (2:1:1), the addition of NPK 18-05-09 formulation Forth CoteR is not necessary to obtain Agonandra brasiliensis plants of good morphological quality. Substrate 3, composed of soil + CRH (3:1), with or without the addition of NPK 18-05-09 formulation Forth CoteR, is not recommended for producing plants of Agonandra brasiliensis, due to the slow development and reduced morphological quality of the plants.

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