scholarly journals Comparison of Honey versus Polylactide Anti-Adhesion Barrier on Peritoneal Adhesion and Healing of Colon Anastomosis in Rabbits

2019 ◽  
Vol 7 (10) ◽  
pp. 1597-1601 ◽  
Author(s):  
Ali Reza Negahi ◽  
Parisa Hosseinpour ◽  
Mohammad Vaziri ◽  
Hamed Vaseghi ◽  
Pedram Darvish ◽  
...  
Keyword(s):  
Intestinal Anastomosis ◽  
Adhesion Formation ◽  
Postoperative Adhesion ◽  
Control Group ◽  
Colon Anastomosis ◽  
Human Beings ◽  
Peritoneal Adhesion ◽  
Adhesion Barrier ◽  
Anastomosis Leakage ◽  
Barrier Film

BACKGROUND: Postoperative adhesion is still a consequence of intra-abdominal surgeries, which results in bowel obstruction and abdominopelvic pain. Bowel anastomosis as a common abdominal surgery has the incidence of leakage in up to 30% of patients that increase morbidity and mortality. Due to similar pathways of adhesion formation and wound healing, it is important to find a way to reduce adhesions and anastomosis leakage. AIM: This study was designed to compare antiadhesive as well as anastomosis healing improvement effect of honey and polylactide anti-adhesive barrier film. METHODS: Forty-five rabbits divided into three groups of honey, adhesion barrier film, and control group in an animal study. Under a similar condition, rabbits underwent resection and anastomosis of cecum under general anaesthesia. In the first group, honey was used at the anastomosis site, in the second one polylactide adhesion barrier film utilised, and the third one was the control group. Adhesion, as well as anastomosis leakage, was assessed after 21 days. Data were analysed using the Statistical Package for Social Scientists (SPSS) for Windows version 25. RESULTS: Three groups of 15 rabbits were studied. The results showed that mean peritoneal adhesion score (PAS) was lower in the honey group (1.67) in comparison to the adhesion barrier film group (3.40) and the control group (6.33). CONCLUSION: Bio-absorbable polylactide barrier has an anti-adhesion effect but is not suitable for intestinal anastomosis in rabbits. Further studies needed to evaluate these effects on human beings.

scholarly journals Evaluation of adhesion barrier types in a rat hepatectomy-induced adhesion model

2020 ◽  
Author(s):  
Atsushi Shimizu ◽  
Miho Kai ◽  
Masako Tasaki ◽  
Naotaka Chino ◽  
Kiyoshi Hasegawa ◽  
...  
Keyword(s):  
Foreign Body Reaction ◽  
Histopathological Examination ◽  
Adhesion Formation ◽  
Mesothelial Cells ◽  
Control Group ◽  
Sprague Dawley ◽  
Liver Lobe ◽  
Adhesion Barrier ◽  
Sprague Dawley Rats ◽  
Adhesion Model

Abstract Background: Adhesion formation after hepatectomy creates problems for repeat hepatectomy. This study aimed to compare the effectiveness of a spray (AdSpray) and sheet adhesion barrier (Seprafilm) in a rat hepatectomy-induced adhesion model.Methods: Thirty male Sprague-Dawley rats underwent partial resection of the left lateral liver lobe. They were randomly assigned to control (n=10), AdSpray (n=10), and Seprafilm groups. Seven days after surgery, the animals were sacrificed, and adhesions at the hepatic resection surface were blindly evaluated.Results: In the control group, adhesions were formed in all 10 animals (100%), with a 69% adhesion extent (mean). In the AdSpray group, the incidence of adhesions (40%) and the adhesion extent (mean, 10%) were significantly lower than in the control group. In the Seprafilm group, the adhesion extent (mean, 30%) was significantly lower than in the control group. As for histopathological examination, animals in the AdSpray group showed a similar healing profile to that of the control group without delayed healing and regeneration of mesothelial cells. In contrast, the Seprafilm group showed ongoing foreign body reaction to Seprafilm, and regeneration of mesothelial cells was immature at 7 days.Conclusions: Both the spray-type gel and sheet adhesion barriers significantly reduced adhesion formation after hepatectomy. The spray-type adhesion barrier caused no adverse events and induced favorable healing. These adhesion barriers may be effective in hepatectomy.

scholarly journals The Inhibitory Effects of Naringin in a Rat Model of Postoperative Intraperitoneal Adhesion Formation

2022 ◽  
Vol 2022 ◽  
pp. 1-10
Author(s):  
Xiaoqiang Shi ◽  
Yunhua Wu ◽  
Enmeng Li ◽  
Li Zhang ◽  
Yanfei Ma ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rat Model ◽  
Adhesion Formation ◽  
Drug Candidate ◽  
Control Group ◽  
High Dose ◽  
Collagen Deposition ◽  
Peritoneal Adhesion ◽  
Inflammatory Reactions ◽  
Intraperitoneal Adhesions

Background. Many attempts have been made to inhibit the formation of postoperative intraperitoneal adhesions, but the results have been discouraging. Therefore, the identification of effective preventative measures or treatments is of great importance. In this study, the substantial potential of naringin (NG) to reduce peritoneal adhesions was validated in a rat model. Materials and Methods. A rat peritoneal adhesion model was established by abrasion of the cecum and its opposite intraperitoneal region under aseptic surgical conditions. After the operation, three groups of NG-treated rats were given 2 mL of NG by gavage at different concentrations (40, 60, or 80 mg/kg/d). The sham, control, and hyaluronan (HA) groups were given equal volumes of normal saline daily. On the 8th day, all rats were sacrificed 30 min after the administration of an activated carbon solution (10 mL/kg) by oral gavage. Intraperitoneal adhesion formation was adequately evaluated by necropsy, hematoxylin and eosin (HE) staining, Sirius red staining, immunofluorescence staining, enzyme-linked immunosorbent assays, and reactive oxygen species (ROS) probes. The gastrointestinal dynamics of the rats were assessed on the basis of a small intestinal charcoal powder propulsion test and the detection of motilin and gastrin levels in serum. Results. Intraperitoneal adhesions were markedly reduced in the group of rats receiving high-dose NG. Compared with the control group, the high-dose NG group showed clear reductions in inflammatory reactions, oxidative stress, collagen deposition, and fibroblast formation in the adhesion tissue and enhanced gastrointestinal dynamics ( P  < 0.05). Conclusion. NG alleviated the severity of intraperitoneal adhesions in a rat model by reducing inflammation, oxidative stress, collagen deposition, and fibroblast formation, highlighting the potential of NG as a drug candidate to prevent postoperative peritoneal adhesion formation.

scholarly journals Effect of Emodin on Preventing Postoperative Intra-Abdominal Adhesion Formation

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Guangbing Wei ◽  
Yunhua Wu ◽  
Qi Gao ◽  
Cancan Zhou ◽  
Kai Wang ◽  
...  
Keyword(s):  
Adhesion Formation ◽  
Major Complication ◽  
Chinese Herbs ◽  
Postoperative Adhesion ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Abdominal Adhesions ◽  
Abdominal Adhesion ◽  
Naturally Occurring ◽  
Oral Doses

Background. Postoperative intra-abdominal adhesions are a major complication after abdominal surgery. Although various methods have been used to prevent and treat adhesions, the effects have not been satisfactory. Emodin, a naturally occurring anthraquinone derivative and an active ingredient in traditional Chinese herbs, exhibits a variety of pharmacological effects. In our study, we demonstrated the effect of emodin treatment on preventing postoperative adhesion formation. Materials and Methods. A total of 48 rats were divided into six groups. Abdominal adhesions were created by abrasion of the cecum and its opposite abdominal wall. In the experimental groups, the rats were administered daily oral doses of emodin. On the seventh day after operation, the rats were euthanized, and blood and pathological specimens were collected. Abdominal adhesion formation was evaluated by necropsy, pathology, immunohistochemistry, Western blot, and enzyme-linked immunosorbent assay analyses. Results. Abdominal adhesions were markedly reduced by emodin treatment. Compared with the control group, collagen deposition was reduced and the peritoneal mesothelial completeness rate was higher in the emodin-treated groups. Emodin had anti-inflammatory effects, reduced oxidative stress, and promoted the movement of the intestinal tract (P<0.05). Conclusion. Emodin significantly reduced intra-abdominal adhesion formation in a rat model.

scholarly journals Effect of ligustrazine nanoparticles on Th1/Th2 balance by TLR4/MyD88/NF-κB pathway in rats with postoperative peritoneal adhesion

BMC Surgery ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Lili Yang ◽  
Ziyu Lian ◽  
Bin Zhang ◽  
Zhengjun Li ◽  
Li Zeng ◽  
...  
Keyword(s):  
Critical Role ◽  
Adhesion Formation ◽  
Sodium Hyaluronate ◽  
Control Release ◽  
Entrapment Efficiency ◽  
Postoperative Adhesion ◽  
Sprague Dawley ◽  
Peritoneal Adhesion ◽  
Western Blot Assay

Abstract Background Postoperative peritoneal adhesion (PPA) is regarded as fibrous bands connecting both injured abdominal wall and organs or adjacent tissues. It is associated with T helper (Th)1 and Th2 differentiation. However, the critical role of the immunopathogenesis of adhesion formation was precisely unknown. The aim of this study was to investigate the effect of a new agent polylactic acid (PLA) nanoparticles loaded with ligustrazine, that is, ligustrazine nanoparticles (LN) on PPA and identify the potential mechanism. Methods Twenty-four Sprague–Dawley rats were randomly divided into the sham, model, LN, and sodium hyaluronate (SH) groups. The structure of LN, including entrapment efficiency (EE) and loading capacity (LC), and in vitro drug release were calculated. Adhesions were scored and the Masson's trichrome staining was used to determine the collagen deposition. The expressions of TLR4, MyD88, and NF-κB were measured by qRT-PCR, immunohistochemistry, and western blot assay. Moreover, Th1-related cytokines (IFN-γ, IL-12), Th2-related cytokines (IL-4, IL-6) in the cecum tissue and serum were conducted by ELISA. Results LN had good EE, LC, and control-release delivery characters with fairly uniform diameter and spherical morphology. It could effectively prevent adhesion formation after surgery. Besides, it could reduce collagen fibers accumulation, downregulate the expression levels of TLR4, MyD88, and NF-κB, and maintain Th1/Th2 balance. Conclusions Ligustrazine nanoparticles had effective effects on Th1/Th2 balance by regulating TLR4/MyD88/NF-κB pathway in PPA rats. It may be served as a promising therapy on postoperative adhesion formation.

scholarly journals Adhesion prevention after endometriosis surgery — results of a randomized, controlled clinical trial with second-look laparoscopy

2021 ◽  
Author(s):  
Bernhard Krämer ◽  
Jürgen Andress ◽  
Felix Neis ◽  
Sascha Hoffmann ◽  
Sara Brucker ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Saline Solution ◽  
Adhesion Formation ◽  
Adhesion Prevention ◽  
Control Group ◽  
Controlled Clinical Trial ◽  
Randomized Controlled Clinical Trial ◽  
Adhesion Barrier ◽  
Randomized Controlled ◽  
Endometriosis Surgery

Abstract Purpose Adhesion formation after endometriosis surgery is a severe problem affecting up to 90% of patients. Possible complications include chronic pain, ileus, and secondary infertility. Therefore, effective adhesion prophylaxis is desirable, for which the adhesion barrier 4DryField® PH is evaluated in the present clinical study. It is a starch-based powder that forms a gel after irrigation with saline solution and thus separates surgical sites as physical barrier for adhesion prevention. Methods Fifty patients with extensive and deep infiltrating endometriosis were included in this prospective, randomized, controlled clinical trial with two-staged laparoscopic approach. The patients were randomized into two groups, one receiving 4DryField® PH and the other irrigation with saline solution for adhesion prevention. Adhesion formation was directly scored during second-look interventions considering incidence, extent, and severity. Adhesion prevention treatment in the second surgery was performed corresponding to the first intervention to evaluate the long-term outcome in the later course. Results Both groups were comparable with respect to relevant patient parameters. Severity and extent of adhesions were significantly reduced by 85% in the 4DryField® PH group compared to the control group (mean total adhesion score 2.2 vs. 14.2; p = 0.004). Incidence of adhesion formation based on the number of affected sites was significantly reduced by 53% in the intervention vs. control group (mean 1.1 vs. 2.3 sites; p = 0.004). Follow-up of secondary endpoints is not yet completed; results will become available at a later stage. Conclusion Adhesion formation could be reduced significantly by 85% by application of the adhesion barrier 4DryField® PH. Trial registration Trial registration main ID: DRKS00014720, secondary ID: U1111-1213-4142; date of registration 09th May 2018.

Decrease in VEGF-Induced Pericardial Adhesion Formation Using Bevacizumab After Surgery

2018 ◽  
Vol 26 (1) ◽  
pp. 21-26 ◽  
Author(s):  
Hosseinali Jelveh Moghaddam ◽  
Marjan Aghajani ◽  
Ehsan Raeis-Abdollahi ◽  
Mahdieh Faghihi ◽  
Ali Dabbagh ◽  
...  
Keyword(s):  
Inflammatory Responses ◽  
Adhesion Formation ◽  
High Sensitivity ◽  
Postoperative Adhesion ◽  
Control Group ◽  
C Reactive Protein ◽  
Protein Levels ◽  
Reactive Protein ◽  
Cd 31 ◽  
Pericardial Adhesions

Objectives. Some degrees of postoperative cardiac adhesions occur in response to the first cardiac surgery in patients that may limit surgeons for subsequent operations and increase the risk of heart injury. In this article, we established a model of postoperative pericardial adhesions, and because vascular endothelial growth factor (VEGF) seems to initiate adhesion formation through inflammatory responses, we used an anti-VEGF antibody, that is, bevacizumab, to examine its effects on postoperative adhesion formation. Methods. Twenty Wistar rats were divided in 2 groups: control and bevacizumab. After chest opening, pericardial sac was opened and the heart was fully exposed. In the bevacizumab group, bevacizumab (2.5 mg/kg) was applied locally on the heart and then the chest was closed. The control group received saline solution as placebo. After 42 days, high-sensitivity C-reactive protein in peripheral blood was measured, and re-sternotomy was performed to measure severity of pericardial adhesions. Then, the hearts were collected from all rats to evaluate percentage of CD-31-positive cells (as a marker of angiogenesis) using immunohistochemical staining. Results. When the bevacizumab group was compared with the control group, we found that the mean score of adhesion (0.89 ± 0.38 vs 2.56 ± 0.41) and CD-31 expression (27.45 ± 3.75% vs 56.26 ± 1.98%) was decreased significantly after bevacizumab administration. However, we did not find any difference in high-sensitivity C-reactive protein levels of control and bevacizumab animals. Conclusion. In the current study, bevacizumab administration could effectively reduce adhesion formation after first sternotomy by preventing VEGF-induced angiogenesis through CD-31 downregulation.

scholarly journals Evaluation of adhesion barrier types in a rat hepatectomy-induced adhesion model

2020 ◽  
Author(s):  
Atsushi Shimizu ◽  
Miho Kai ◽  
Masako Tasaki ◽  
Naotaka Chino ◽  
Kiyoshi Hasegawa ◽  
...  
Keyword(s):  
Animal Studies ◽  
Histopathological Examination ◽  
Adhesion Formation ◽  
Mesothelial Cells ◽  
Control Group ◽  
Sprague Dawley ◽  
Liver Lobe ◽  
Adhesion Barrier ◽  
Sprague Dawley Rats ◽  
Adhesion Model

Abstract Background: Adhesion formation after hepatectomy creates problems for repeat hepatectomy. This study aimed to compare the effectiveness of a spray (AdSpray) and sheet adhesion barrier (Seprafilm) in a rat hepatectomy-induced adhesion model.Methods: Thirty male Sprague-Dawley rats underwent partial resection of the left lateral liver lobe. They were randomly assigned to control (n=10), AdSpray (n=10), and Seprafilm groups. Seven days after surgery, the animals were sacrificed, and adhesions at the hepatic resection surface were blindly evaluated.Results: In the control group, adhesions were formed in all 10 animals (100%), with a 69% adhesion extent (mean). In the AdSpray group, the incidence of adhesions (40%) and the adhesion extent (mean, 10%) were significantly lower than in the control group (incidence; p = 0.0147, adhesion extent; p = 0.0007). In the Seprafilm group, the incidence of adhesions was 70%. The adhesion extent of Seprafilm (mean, 30%) was significantly lower than in the control group (p = 0.0492). No significant differences were observed between the AdSpray and Seprafilm groups. As for histopathological examination, animals in the AdSpray group showed a similar healing profile to that of the control group without delayed healing and regeneration of mesothelial cells. In contrast, the Seprafilm group showed ongoing foreign body reaction to Seprafilm, and regeneration of mesothelial cells was immature at 7 days.Conclusions: Both the spray-type gel and sheet adhesion barriers significantly reduced adhesion formation after hepatectomy. The spray-type adhesion barrier caused no adverse events and induced favorable healing. These adhesion barriers may be effective in hepatectomy. Further animal studies and clinical trials are required to determine their benefits in clinical use.

scholarly journals Methylene blue 1% solution on the prevention of intraperitoneal adhesion formation in a dog model

2013 ◽  
Vol 43 (9) ◽  
pp. 1668-1674
Author(s):  
Marco Augusto Machado Silva ◽  
Olízio Claudino da Silva ◽  
Luiz Antônio Franco da Silva ◽  
Raquel Cunha Ribeiro ◽  
Paula Marina Brito Jorge ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Surgical Intervention ◽  
Adhesion Formation ◽  
Falciform Ligament ◽  
Postoperative Adhesion ◽  
Control Group ◽  
Surgical Trauma ◽  
The Mean ◽  
Trauma Model ◽  
Treatment Control Group

Intraperitoneal adhesions usually are formed after abdominal surgeries and may cause technical difficulties during surgical intervention, chronic abdominal pain and severe obstructions of the gastrointestinal tract. The current study aimed to evaluate the efficacy of methylene blue (MB) 1% solution on the prevention of intraperitoneal postsurgical adhesion formation in a canine surgical trauma model. Twenty bitches were submitted to falciform ligament resection, omentectomy, ovariohysterectomy and scarification of a colonic segment. Prior to abdominal closure, 10 bitches received 1mg kg-1 MB intraperitoneally (MB group) and 10 bitches received no treatment (control group, CT). On the 15th postoperative day the bitches were submitted to laparoscopy to assess adhesions. The mean adhesion scores were 13.9 (±5.6) for MB group and 20.5 (±6.4) for the CT group (P=0,043). In conclusion, the 1% MB solution was efficient on the prevention of intraperitoneal postoperative adhesion formation in bitches, especially those involving the colonic serosa.

scholarly journals The Improvement of Laparoscopic Surgical Skills Obtained by Gynecologists after Ten Years of Clinical Training Can Reduce Peritoneal Adhesion Formation during Laparoscopic Myomectomy: A Retrospective Cohort Study

2017 ◽  
Vol 2017 ◽  
pp. 1-5
Author(s):  
Valerio Mais ◽  
Michele Peiretti ◽  
Luigi Minerba
Keyword(s):  
Clinical Training ◽  
Adhesion Formation ◽  
Laparoscopic Myomectomy ◽  
Postoperative Adhesion ◽  
Peritoneal Adhesion ◽  
Laparoscopic Skills ◽  
Operative Laparoscopy ◽  
Second Look ◽  
Main Variable ◽  
Peritoneal Adhesion Formation

Objective. To evaluate if improvement of laparoscopic skills can reduce postoperative peritoneal adhesion formation in a clinical setting. Study Design. We retrospectively evaluated 25 women who underwent laparoscopic myomectomy from January 1993 to June 1994 and 22 women who underwent laparoscopic myomectomy from March 2002 to November 2004. Women had one to four subserous/intramural myomas and received surgery without antiadhesive agents or barriers. Women underwent second-look laparoscopy for assessment of peritoneal adhesion formation 12 to 14 weeks after myomectomy. Adhesions were graded according to the Operative Laparoscopy Study Group scoring system. The main variable to be compared between the two cohorts was the proportion that showed no adhesions at second-look laparoscopy. Results. Demographic and surgical characteristics were similar between the two cohorts. No complications were observed during surgery. No adverse events were recorded during postoperative course. At second-look laparoscopy, a higher proportion of adhesion-free patients was observed in women who underwent laparoscopic myomectomy from March 2002 to November 2004 (9 out of 22) compared with women who underwent the same surgery from January 1993 to June 1994 (3 out of 25). Conclusion. The improvement of surgeons’ skills obtained after ten years of surgery can reduce postoperative adhesion formation.

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