Leptin and the GA genotype of rs2167270 of the LEP gene increase the risk of prediabetes

MC4R, LEP, and LEPR genes are involved in the hypothalamic leptin-melanocortin regulation pathway, which is important for energy homeostasis. Our study aimed to evaluate the associations between the MC4R rs17782313, LEP rs7799039, and LEPR rs1137101 polymorphisms with obesity-related parameters in childhood and adulthood. The data were obtained from the Kaunas Cardiovascular Risk Cohort study, which started in 1977 with 1082 participants aged 12–13 years. In 2012–2014, the follow-up survey was carried out. Genotype analysis of all respondents (n = 509) aged 48–49 years was performed for the gene polymorphisms using Real-Time Polymerase Chain Reaction. Anthropometric measurements were performed in childhood and adulthood. In childhood, only skinfold thicknesses were associated with gene variants being the lowest in children with MC4R TT genotype and LEP AG genotype. In adulthood, odds of obesity and metabolic syndrome was higher in MC4R CT/CC genotype than TT genotype carriers (OR 1.8; 95% CI 1.2–2.8 and OR 1.6; 95% CI 1.1–2.4, respectively). In men, physical activity attenuated the effect of the MC4R rs17782313 on obesity. The LEP GG genotype was associated with higher BMI, waist circumference, and visceral fat level only in men. No associations of the LEPR rs1137101 polymorphisms with anthropometric measurements and leptin level were found. In conclusion, the associations of the MC4R and LEP gene polymorphisms with obesity-related parameters strengthened with age.

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Mutations in Leptin (LEP) Gene Are Associated with Carcass and Meat Quality Traits in Crossbreed Rabbits

Animal Biotechnology ◽

10.1080/10495398.2017.1347881 ◽

2017 ◽

Vol 29 (2) ◽

pp. 153-159 ◽

Cited By ~ 3

Author(s):

Lukasz Migdal ◽

Konrad Koziol ◽

Sylwia Palka ◽

Wladyslaw Migdal ◽

Tomasz Zabek ◽

...

Keyword(s):

Meat Quality ◽

Quality Traits ◽

Meat Quality Traits ◽

Lep Gene

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Effect of G2548A polymorphism in the leptin gene on the BMI level in human population

Acta Agraria Debreceniensis ◽

10.34101/actaagrar/68/1762 ◽

2016 ◽

pp. 5-10

Author(s):

Kritína Candráková ◽

Anna Trakovická

Keyword(s):

Human Population ◽

Genetic Relatedness ◽

Heterozygous Genotype ◽

Dominant Form ◽

Snp Polymorphism ◽

Pcr Rflp ◽

Pcr Products ◽

Agarose Electrophoresis ◽

Prevalence Of Obesity ◽

Lep Gene

The polymorphism in leptin (LEP 2548A) seems to influence obesity among others genes. The aim of this study is to investigate the effect of the G2548A polymorphism on body mass index. We included 79 people from Slovakia with some genetic relatedness and used barrels kit to isolate the genomic DNA from an adenoblast swab- from the salivary. PCR products were amplified by pursued polymorphisms and G2548A, we restriction-analyzed them and then we identified the specific fragments describing the presence of chosen SNP polymorphism by the agarose electrophoresis, to analyze SNP polymorphism by PCR-RFLP method. The LEP gene had increased frequency of G allele (0.5506). The most common genotype occurring in the gene LEP was heterozygous genotype (AG) and the least frequent genotype in LEP was AA (0.1899). Taking the age into account the BMI is higher if the G allele occurs in the LEP gene. Moreover, if the G allele genotype was situated in dominant form, then the highest average BMI was present. According to the results we can assume that the AA genotype (LEP) has a protective effect on the prevalence of obesity compared to the other genotypes.

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Leptin G-2548A Gene Polymorphism is Positively Associated with Increased Plasma Leptin and Glucose Levels in Obese Saudi Patients Irrespective Status of Blood Pressure

10.21203/rs.3.rs-148642/v2 ◽

2021 ◽

Author(s):

Lotfi S. Bin Dahman ◽

Nasser M. Al-Daghri

Keyword(s):

Blood Pressure ◽

Gene Polymorphism ◽

Plasma Glucose ◽

Hdl Cholesterol ◽

Cross Sectional Study ◽

Cross Sectional ◽

Glucose Levels ◽

Lep Gene ◽

Plasma Leptin ◽

Saudi Patients

Abstract The association between LEP G-2548A gene polymorphism with increased plasma leptin and glucose levels and blood pressure in a sample of obese Saudi patients has been evaluated. This is a cross-sectional study involved 206 Saudi adult subjects (94 males and 112 females), randomly selected from the primary health care centers, Riyadh, Saudi Arabia. The study sample was categorized into three groups: 50 normotensive ND controls (age: 47.9±5.4 yr.; BMI 22.9±2.1 Kg/m2), 80 obese normotensive ND (age: 47.7±6.0 yr.; BMI 34.1±4.2 Kg/m2) and 76 obese hypertensive with T2D patients (age: 49.4±5.9 yr.; BMI: 35.1±4.7 Kg/m2). Analyses of LEP G-2548A gene polymorphism were determined using polymerase chain reaction (PCR), followed by restriction fragment length polymorphism (RFLP) with 2U of HhaI restriction enzyme. Plasma leptin and insulin levels were measured using the Luminex instrument. Fasting plasma glucose, total cholesterol, HDL-cholesterol, and triglycerides were measured using a chemical autoanalyzer Konelab machine. Also, blood pressure and anthropometric data were measured. The association analysis with metabolic parameters showed that homozygous AA of the LEP gene had significantly higher plasma glucose levels and HOMA-IR compared with homozygous GG (6.8±0.55 vs. 5.8±0.30; p< 0.04; 4.1±0.84 vs. 2.6±0.67; p=0.03) respectively. Besides, heterozygous GA had significantly higher plasma leptin levels compared with homozygous GG (40.0±2.6 vs. 29.6±2.6; P= 0.04). GA, AA, GA+AA genotypes of the LEP G-2548A gene polymorphism are more prevalent among individuals with hyperglycemia (OR= 3.7, 95% CI= 1.6 to 8.4, P= 0.001; OR= 3.2, 95% CI= 1.2 to 8.6, P= 0.03; OR= 3.5, 95%CI= 1.6 to 7.7, P= 0.001) respectively. A allele of the LEP gene is more prevalent among subjects with hyperglycemia (OR= 1.9, 95%CI= 1.2 to 3.0, P=0.006). G-2548A variant of the LEP gene may not be considered as a genetic risk factor for hypertension in Saudi obese patients. However, the genotypes (GA and AA) and -2548AA allele of this gene may represent important risk factors predisposing healthy subjects to develop T2DM irrespective of the status of blood pressure.

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Genetic Characterization of Thyroglobulin and Leptin Genes in Pasundan Cattle at West Java

Buletin Peternakan ◽

10.21059/buletinpeternak.v43i1.38227 ◽

2019 ◽

Vol 43 (1) ◽

Author(s):

Widya Pintaka Bayu Putra ◽

Saiful Anwar ◽

Syahruddin Said ◽

Romanos Albert Adhitya Indarto ◽

Putri Wulandari

Keyword(s):

Genetic Characterization ◽

Rare Allele ◽

Basic Information ◽

Dominant Allele ◽

West Java ◽

Pcr Rflp ◽

Molecular Selection ◽

Lep Gene ◽

Informative Content

The Thyroglobulin (TG) and Leptin (LEP) genes are two candidate genes that widely used for molecular selection to improve carcass traits in beef cattle. This research was carried out to identify the genetic characterization of TG and LEP genes from 47 heads of Pasundan cows at West Java using PCR-RFLP method. Research shows that TG gene of Pasundan cattle is monomorphic with C allele as the dominant allele (1.00). However, LEP gene of Pasundan cattle is polymorphic with C allele as the dominant allele (0.98) and T as the rare allele (0.02). The polymorphic informative content (PIC) and numberof effective allele (ne) values in the LEP gene in the animal studied were 0.04 and 1.04 respectively. It was concluded that TG/BstYI and LEP/Sau3AI gene in the present study can not be used as molecular selection in Pasundan cattle. These results are important as the basic information for preparing the molecular selection program in the future.

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INFLUENCE OF LEPTIN (LEP) GENE POLYMORPHISM ON DAIRY AND MEAT PRODUCTIVITY OF HOLSTEIN HEIFERS

Scientific Notes Kazan Bauman State Academy of Veterinary Medicine ◽

10.31588/2413-4201-1883-245-1-24-28 ◽

2021 ◽

Vol 245 (1) ◽

pp. 24-28

Author(s):

E.R. Gaynutdinova ◽

◽

N.Yu. Safina ◽

Sh.K. Shakirov ◽

M.I. Varlamova ◽

...

Keyword(s):

Gene Polymorphism ◽

Holstein Cows ◽

Holstein Cattle ◽

Frequency Of Occurrence ◽

Dna Testing ◽

Economic Traits ◽

Blood Samples ◽

Testing Frequency ◽

Lep Gene ◽

Meat Productivity

The aim of this study was to research the association between leptin gene polymorphism in Holstein cows with their dairy and meat traits of productivity. To conduct DNA testing on the LEP gene blood samples were selected from 228 Holstein heifers. Animal genotyping according to the leptin gene was performed by AC-PCR. All possible genotypes of the LEP gene were identified in the course of DNA testing. Frequency of occurrence of genotypes and alleles was ranked as fol-lows: CC – 32.5 %, TC – 49.1 %, TT – 18.4 % and C – 0.57, T – 0.43. The results of this study show that animals with genotype TT of LEP gene can be useful in breeding for the improvement of population of Holstein cattle by economic traits associated to the dairy and meat productivity. This research was supported by the FASO Russia project АААА-А18-118031390148-1.

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THE LEPTIN GENE IS A MARKER FOR THE CELL THERAPY OF METABOLIC SYNDROME

Biomeditsina ◽

10.33647/2074-5982-15-3-12-22 ◽

2019 ◽

pp. 12-22

Author(s):

N. V. Petrova

Keyword(s):

Gene Expression ◽

Metabolic Syndrome ◽

Cell Therapy ◽

Bone Marrow Cells ◽

Liver And Pancreas ◽

A Cell ◽

Polymerase Chain ◽

Lep Gene ◽

The Brain

It is shown that the level of the Lep gene expression is a marker for B/Ks-Leprᵈᵇ/+ mice, which line serves as an optimal model for describing metabolic syndrome (MS) in preclinical studies. Mice were transplanted with cultured isogenic bone marrow cells (BMC) from heterozygous db/+ donors. The recipients were divided into two groups according to an early or advanced stage of MS development. We analyzed the expression of the Lep gene on the 3rd, 8th and 14th day following the administration of stem BMCs in the brain, liver and pancreas cells by polymerase chain reaction (PCR) in real time. The Lep gene expression was evaluated in terms of the number of cDNA copies. According to our data, leptin is a complete regulator of metabolic processes due to its effect on the hypothalamus, which, together with the hippocampus, controls the production of acetylcholine and insulin in the brain. We have proven the role of the Lep gene as a quantitative criterion for evaluating the effi cacy of a cell therapy in MS.

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Associations of ADIPOQ and LEP Gene Variants with Energy Intake: A Systematic Review

Nutrients ◽

10.3390/nu11040750 ◽

2019 ◽

Vol 11 (4) ◽

pp. 750

Author(s):

Caroline Kroll ◽

Silmara Mastroeni ◽

Paul Veugelers ◽

Marco Mastroeni

Keyword(s):

Systematic Review ◽

Energy Intake ◽

Latin American ◽

Positive Association ◽

Bibliographic Databases ◽

Gene Variants ◽

Case Control Studies ◽

Cross Sectional ◽

Adipoq Gene ◽

Lep Gene

This systematic review aims to evaluate the association of adiponectin (ADIPOQ) and leptin (LEP) gene variants with energy intake. Cross-sectional, cohort, and case–control studies that reported an association of leptin and/or adiponectin gene variants with energy intake were included in this review. Human studies without any age restrictions were considered eligible. Detailed individual search strategies were developed for each of the following bibliographic databases: Cochrane, Latin American and Caribbean Center on Health Sciences Information (LILACS), PubMed/MEDLINE, Scopus, and Web of Science. Risk of bias assessment was adapted from the Downs and Black scale and was used to evaluate the methodology of the included studies. Seven studies with a pooled population of 2343 subjects were included. The LEP and ADIPOQ gene variants studied were LEP-rs2167270 (k = 1), LEP-rs7799039 (k = 5), ADIPOQ-rs2241766 (k = 2), ADIPOQ-rs17300539 (k = 1), and ADIPOQ marker D3S1262 (k = 1). Two of the seven studies reviewed demonstrated a positive association between the LEP-rs7799039 polymorphism and energy intake. Two other studies—one involving a marker of the ADIPOQ gene and one examining the ADIPOQ-rs17300539 polymorphism—also reported associations with energy intake. More research is needed to further elucidate the contributions of genetic variants to energy metabolism.

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