scholarly journals INFLUENCE OF LEPTIN (LEP) GENE POLYMORPHISM ON DAIRY AND MEAT PRODUCTIVITY OF HOLSTEIN HEIFERS

2021 ◽  
Vol 245 (1) ◽  
pp. 24-28
Author(s):  
E.R. Gaynutdinova ◽  
◽  
N.Yu. Safina ◽  
Sh.K. Shakirov ◽  
M.I. Varlamova ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Holstein Cows ◽  
Holstein Cattle ◽  
Frequency Of Occurrence ◽  
Dna Testing ◽  
Economic Traits ◽  
Blood Samples ◽  
Testing Frequency ◽  
Lep Gene ◽  
Meat Productivity

The aim of this study was to research the association between leptin gene polymorphism in Holstein cows with their dairy and meat traits of productivity. To conduct DNA testing on the LEP gene blood samples were selected from 228 Holstein heifers. Animal genotyping according to the leptin gene was performed by AC-PCR. All possible genotypes of the LEP gene were identified in the course of DNA testing. Frequency of occurrence of genotypes and alleles was ranked as fol-lows: CC – 32.5 %, TC – 49.1 %, TT – 18.4 % and C – 0.57, T – 0.43. The results of this study show that animals with genotype TT of LEP gene can be useful in breeding for the improvement of population of Holstein cattle by economic traits associated to the dairy and meat productivity. This research was supported by the FASO Russia project АААА-А18-118031390148-1.

DNA TESTING OF THE GPX-1 GENE POLYMORPHISM IN CATTLE

2020 ◽  
pp. 37-39
Author(s):  
Н.Ю. САФИНА ◽  
Ш.К. ШАКИРОВ ◽  
Э.Р. ГАЙНУТДИНОВА ◽  
З.Ф. ФАТТАХОВА
Keyword(s):  
Hydrogen Peroxide ◽  
Glutathione Peroxidase ◽  
Gene Polymorphism ◽  
Holstein Cattle ◽  
Dna Testing ◽  
Gene Encoding ◽  
Glutathione Peroxidase 1 ◽  
Antioxidant Protein ◽  
Pcr Rflp ◽  
Hardy Weinberg Equilibrium

Глутатионпероксидаза-1 (GPX-1) является геном, кодирующим внутриклеточный антиоксидантный белок, который ферментативно восстанавливает перекись водорода до воды, отвечает за поддержание целостности биомолекул. Целью работы было изучение полиморфизма гена глутатионпероксидаза-1 у крупного рогатого скота голштинской породы. В ходе ДНК-тестирования 148 коров методом ПЦР-ПДРФ установлено, что исследуемая популяция полиморфна по гену GPX-1. Частота встречаемости аллелей составила: С — 0,503 и Т — 0,497; генотипов ТС — 60,1%, СС — 20,3 и ТТ — 19,6%. Исследованная порода находилась в пределах генетического равновесия, согласно закону Харди-Вайнберга (Р˂0,05). Полученные данные свидетельствовали о биоразнообразии изучаемой популяции голштинского крупного рогатого скота. Glutathione peroxidase-1 (GPX-1) is a gene encoding an intracellular antioxidant protein that enzymatically reduces hydrogen peroxide to water, is responsible for maintaining the integrity of biomolecules. The aim of this study was to examine the glutathione peroxidase-1 gene polymorphism in the Holstein cattle stock. During the DNA testing of 148 cows by using the PCR- RFLP method it was found that the population under study is polymorphic by the GPX-1 gene. Allele frequency was: С — 0.503 and Т — 0.497; genotype frequency was: ТС – 60.1, СС — 20.3 and ТТ — 19.6 %. Examined breed was within the Hardy-Weinberg equilibrium (Р>0.05). The findings indicate the biodiversity of the population of Holstein cattle.

scholarly journals Influence of polymorphism of complex of csn3 and lgb genes on the dairy productivity in holstein cattle

2020 ◽  
pp. 64-67
Author(s):  
Natalia Yurevna Safina ◽  
Elza Ravilevna Gaynutdinova ◽  
Farida Fattihovna Zinnatova ◽  
Shamil Kasimovich Shakirov ◽  
Gazimzyan Salimovich Sharafutdinov
Keyword(s):  
Mass Fraction ◽  
Allelic Polymorphism ◽  
Holstein Cattle ◽  
Frequency Of Occurrence ◽  
Dna Testing ◽  
Minimum Level ◽  
Milk Productivity ◽  
Pcr Rflp ◽  
High Level ◽  
Beta Lactoglobulin

The study presents the results obtained in the course of DNA testing of allelic polymorphism by PCR-RFLP on CSN3 and LGB genes and the associations of their complex genotypes to the indicators of milk productivity of Holstein cow-heifers.Genetic polymorphism was detected by digestion with the endonucleases Hinf I (CSN3) and Hae III (LGB). All integrated CSN3/LGB genotypes were identified during the work. The maximum frequency of occurrence is observed in a combination of heterozygous AB/AB genotypes – 120 animals (25.5%), which is the fourth part of the studied cattle population. The group of animals with homozygous BB/BB genotypes of CSN3/LGB genes is the smallest one – 10 animals (2.1%). Milk productivity test, based on the complex CSN3 and LGB genotypes is observed in heifers with an integrated combination of CSN3BBLGBAAshowed that a significantly high level of productivity for 305 days of lactation – 7239.8 kg, and a minimum level of productivity (6222.9 kg) is observed in heifers with the complex CSN3BBLGBAB genotype, compared with indicators of milk yield of cow-heifers with other integrated genotypes. According to the content of the mass fraction of fat, the best indicators were established in the subpopulation of animals with the complex AA/AB genotype – 3.90%, and the worst result was recorded in the group of animals with the complex AB/AA genotype, which amounted to 3.44%. The high content of the mass fraction of protein was observed in individuals by the complexof the CSN3 and LGB genes BB/AA – 3.43%, and a reduced level of protein was observed in heifers with a combination of homozygous AA/AA and BB/BB genotypes by the genes under study – 3.18%. Thus, individuals carrying the homozygous A allele of the kappa-casein (CSN3) gene and the homozygous B allele of the beta-lactoglobulin (LGB) gene in the integrated genotype favorably differed on the main indicators of milk productivity.

scholarly journals Genetic parameters of milk productivity for three lactations of Holstein cattle with different genotypes of LEP gene

2020 ◽  
Vol 27 ◽  
pp. 00061
Author(s):  
Tahir M. Akhmetov ◽  
Natalia Yu. Safina ◽  
Azat M. Alimov ◽  
Margarita I. Varlamova
Keyword(s):  
Milk Yield ◽  
Holstein Cows ◽  
High Yield ◽  
Allelic Polymorphism ◽  
Dna Testing ◽  
Pcr Method ◽  
Lep Gene ◽  
The Republic ◽  
Daily Milk Yield ◽  
Daily Milk

The research presents the findings of DNA testing of allelic polymorphism by the AC-PCR method of the LEP gene. The research was conducted among 172 Holstein cows at Integrated Agricultural Production Centre “Stud farm named after Lenin” in Atninsky district of the Republic of Tatarstan in 2017–2018. All genotypes of the LEP gene were identified through the research. Associations of the leptin gene polymorphism with dynamics of milk production during three lactations of Holstein cows were established. The best indicators for all three lactations were found in a group of animals with the TT genotype of the LEP gene. These individuals are characterized by increased milk yield, a high yield index, and tend to increase the average daily milk yield during three lactations. These individuals are characterized by increased milk yield, a high milk yield index, and tend to increase the average daily milk yield during three lactations.

scholarly journals Effect of the bovine TG5 gene polymorphism on milk- and meat-producing ability

2020 ◽  
Vol 13 (10) ◽  
pp. 2046-2052
Author(s):  
I. Dolmatova ◽  
T. Sedykh ◽  
F. Valitov ◽  
R. Gizatullin ◽  
D. Khaziev ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Fat Metabolism ◽  
Fat Content ◽  
Milk Productivity ◽  
Clear Trend ◽  
Black And White ◽  
Republic Of Bashkortostan ◽  
The Republic ◽  
Productivity Indicators ◽  
Meat Productivity

Aim: This study aimed to determine the effect of thyroglobulin (TG5) gene polymorphism on milk and meat productivity in the various cattle breeds currently bred in the Republic of Bashkortostan. Materials and Methods: The test was performed on dairy cattle of Black-and-White, Bestuzhev, and Simmental breeds, and meat cattle of Hereford and limousine breeds. The purpose of the test was to search for associations between the polymorphic alleles of the thyroglobulin (TG5) gene and economically useful traits. Results: All studied breeds showed a frequency predominance of the TG5C allele (from 0.56 to 0.71). A clear trend of an effect of the genotypes of the TG5 gene on milk-productivity indicators was revealed; cows with the TG5TT genotype have the highest milk yield and fat content in milk. The milk of cows of Bestuzhev and Simmental breeds that possessed this genotype was also characterized by higher protein content. Conclusion: We identified an effect of the polymorphism of the TG5 gene in the Hereford and limousine breeds on fat metabolism intensity indicators, such as fat output and fat content, in the longissimus muscle and in the general sample of ground beef.

scholarly journals Leptin G-2548A Gene Polymorphism is Positively Associated with Increased Plasma Leptin and Glucose Levels in Obese Saudi Patients Irrespective Status of Blood Pressure    

2021 ◽  
Author(s):  
Lotfi S. Bin Dahman ◽  
Nasser M. Al-Daghri
Keyword(s):  
Blood Pressure ◽  
Gene Polymorphism ◽  
Plasma Glucose ◽  
Hdl Cholesterol ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Glucose Levels ◽  
Lep Gene ◽  
Plasma Leptin ◽  
Saudi Patients

Abstract The association between LEP G-2548A gene polymorphism with increased plasma leptin and glucose levels and blood pressure in a sample of obese Saudi patients has been evaluated. This is a cross-sectional study involved 206 Saudi adult subjects (94 males and 112 females), randomly selected from the primary health care centers, Riyadh, Saudi Arabia. The study sample was categorized into three groups: 50 normotensive ND controls (age: 47.9±5.4 yr.; BMI 22.9±2.1 Kg/m2), 80 obese normotensive ND (age: 47.7±6.0 yr.; BMI 34.1±4.2 Kg/m2) and 76 obese hypertensive with T2D patients (age: 49.4±5.9 yr.; BMI: 35.1±4.7 Kg/m2). Analyses of LEP G-2548A gene polymorphism were determined using polymerase chain reaction (PCR), followed by restriction fragment length polymorphism (RFLP) with 2U of HhaI restriction enzyme. Plasma leptin and insulin levels were measured using the Luminex instrument. Fasting plasma glucose, total cholesterol, HDL-cholesterol, and triglycerides were measured using a chemical autoanalyzer Konelab machine. Also, blood pressure and anthropometric data were measured. The association analysis with metabolic parameters showed that homozygous AA of the LEP gene had significantly higher plasma glucose levels and HOMA-IR compared with homozygous GG (6.8±0.55 vs. 5.8±0.30; p< 0.04; 4.1±0.84 vs. 2.6±0.67; p=0.03) respectively. Besides, heterozygous GA had significantly higher plasma leptin levels compared with homozygous GG (40.0±2.6 vs. 29.6±2.6; P= 0.04). GA, AA, GA+AA genotypes of the LEP G-2548A gene polymorphism are more prevalent among individuals with hyperglycemia (OR= 3.7, 95% CI= 1.6 to 8.4, P= 0.001; OR= 3.2, 95% CI= 1.2 to 8.6, P= 0.03; OR= 3.5, 95%CI= 1.6 to 7.7, P= 0.001) respectively. A allele of the LEP gene is more prevalent among subjects with hyperglycemia (OR= 1.9, 95%CI= 1.2 to 3.0, P=0.006). G-2548A variant of the LEP gene may not be considered as a genetic risk factor for hypertension in Saudi obese patients. However, the genotypes (GA and AA) and -2548AA allele of this gene may represent important risk factors predisposing healthy subjects to develop T2DM irrespective of the status of blood pressure.

scholarly journals Genetic variations of the bovine MX1 and their association with mastitis

2017 ◽  
Vol 62 (No. 4) ◽  
pp. 157-167
Author(s):  
N. Chen ◽  
F. Wang ◽  
N. Yu ◽  
Y. Gao ◽  
J. Huang ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Wide Spectrum ◽  
Holstein Cows ◽  
Holstein Cattle ◽  
Nucleotide Polymorphisms ◽  
Coding Region ◽  
Bacterial Strains ◽  
Cattle Breeds ◽  
Chinese Cattle ◽  
Mx1 Gene

The primary agent of mastitis is a wide spectrum of bacterial strains; however, viral-related mastitis has also been reported. The MX dynamin-like GTPase 1 (MX1) gene has been demonstrated to confer positive antiviral responses to many viruses, and may be a suitable candidate gene for the study of disease resistance in dairy cattle. The present study was conducted to investigate the genetic diversity of the MX1 gene in Chinese cattle breeds and its effects on mastitis in Holstein cows. First, polymorphisms were identified in the complete coding region of the bovine MX1 gene in 14 Chinese cattle breeds. An association study was then carried out, utilizing polymorphisms detected in Holstein cows to determine the associations of these single nucleotide polymorphisms (SNPs) with mastitis. We identified 13 previously reported SNPs in Chinese domestic cattle and four of them in Holstein cattle. A novel 12 bp indel was also discovered in Holstein cattle. In addition, haplotype frequencies and linkage disequilibrium of four SNPs detected in Holstein cows were investigated. Analysis of these four SNPs in Chinese Holstein cows revealed two SNPs (g.143181370 T&gt;C and g.143182088 C&gt;T)<br /> significantly (P &lt; 0.05) associated with somatic cell score (SCS). The results indicated that SNPs in the MX1 gene might contribute to the variations observed in the SCS of dairy cattle. Therefore, implementation of these two mutations in selection indexes of the dairy industry might be beneficial by favouring the selection individuals with lower SCS.

scholarly journals Association study of rs1801282 PPARG gene polymorphism and immune cells and cytokine levels in a Spanish pregnant women cohort and their offspring

2020 ◽  
Vol 27 (1) ◽  
Author(s):  
Maria García-Ricobaraza ◽  
Mercedes García-Bermúdez ◽  
Francisco J. Torres-Espinola ◽  
M. Teresa Segura Moreno ◽  
Mathieu N. Bleyere ◽  
...  
Keyword(s):  
Immune System ◽  
Gene Polymorphism ◽  
Cord Blood ◽  
Pregnant Women ◽  
Venous Blood ◽  
Innate Immune ◽  
Peroxisome Proliferator ◽  
Blood Samples ◽  
Inflammatory Reactions ◽  
Immune Parameters

Abstract Background Peroxisome proliferator activated receptor gamma (PPARG) belongs to the nuclear receptor superfamily functioning as transcription factors to regulate cellular differentiation, development and metabolism. Moreover, it has been implicated in the regulation of lipid metabolism, as well as the maturation of monocytes/macrophages and the control of inflammatory reactions. The aim of this study was to evaluate the relationship between the Pro12Ala (rs1808212) PPARG gene polymorphism on immune molecular and cellular components in mothers and their offspring participating in the PREOBE study. Methods DNA from maternal venous blood samples at 24, 34 and 40 gestational weeks, plus cord blood samples was extracted. Pro12Ala PPARG polymorphism genotyping was performed, and immune system markers were analyzed by flow cytometry. Results Study findings revealed no effect of rs1808212 PPARG genotypes on innate immune parameters in mothers and their offspring; however, CD4 + /CD8 + ratio were decreased at 24 and 34 weeks in pregnant women carrying the CG (Pro12Ala) rs1808212 polymorphism, (p = 0,012 and p = 0,030; respectively). Only CD19 levels in peripheral blood were significantly higher at delivery in pregnant women carrying the CC (Pro12Pro) genotype (p ≤ 0.001). Moreover, there were statistically significant differences in leukocytes and neutrophils maternal levels at 34 weeks of gestation, being lower in carriers of Pro12Ala genotype (p = 0.028 and p = 0.031, respectively). Conclusions Results suggest that Pro12Ala PPARG polymorphism may have an effect on some cell and immune parameters in pregnant women during pregnancy and at time of delivery. However, newborn innate immune system does not seems to be influenced by PPARG Pro12Ala polymorphism in cord blood.

Possible Causes and Consequences of the Distribution of Separate Allelic Variants of the LEP Gene in the Groups of Ayrshire and Holstein Cattle

2018 ◽  
Vol 54 (12) ◽  
pp. 1466-1470
Author(s):  
N. V. Kovalyuk ◽  
V. F. Satsuk ◽  
E. V. Machulskaya ◽  
Yu. Yu. Shakhnazarova
Keyword(s):  
Holstein Cattle ◽  
Allelic Variants ◽  
Lep Gene

SP031 Evidence-based guidelines for the pre-analytical phase of DNA, RNA and cell-free DNA testing in blood samples

2013 ◽  
Vol 49 ◽  
pp. S8-S9
Author(s):  
M. Pazzagli ◽  
F. Malentacchi ◽  
R. Wyrich ◽  
C.C. Hartmann ◽  
P. Verderio ◽  
...  
Keyword(s):  
Evidence Based ◽  
Dna Testing ◽  
Cell Free Dna ◽  
Blood Samples ◽  
Free Dna ◽  
Analytical Phase ◽  
Evidence Based Guidelines

scholarly journals Exteriority of Holstein Cattle of Various Origins in the Conditions of the Tyumen Region

2019 ◽  
Author(s):  
Marina Svyazhenina
Keyword(s):  
Dairy Farming ◽  
European Countries ◽  
Holstein Cows ◽  
Holstein Cattle ◽  
Animal Products ◽  
Tyumen Oblast

The modern production of animal products depends largely on the quality of animals. Dairy farming is most often based on the use of Holstein cows as one of the most adapted to industrial conditions[1]. The Tyumen Oblast has been importing the cattle of this breed since 2006, and it was imported from different European countries, as well as was obtained as a result of the absorption crossbreeding of cows of the domestic black-motley breed [2–4]. Since the productivity of animals to a large extent depends not only on the origin, but also on the external qualities of animals, a study of the exteriors of the first exteriors of the Korovogolshtin breed was carried out. In the course of the study, it was found that both the origin and the ”farm” factor were influenced by the exterior quality of the animals. Moreover, the influence of the ”farm” factor was greater than the origin of cows. The cows of their own breeding, obtained as a result of absorption crossbreeding, did not differ significantly from the imported cows by the majority of external indicators. This allows us to conclude that the imports may be replaced by our own reproduction, including the use of absorption crossbreeding of black and motley and Holstein cattle.

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