Exosomal microRNA‑302a promotes trophoblast migration and proliferation, and represses angiogenesis by regulating the expression levels of VEGFA in preeclampsia

IntroductionObesity is a risk factor for type 2 diabetes mellitus (T2DM) and cardiovascular disease. T2DM increases the risk of cardiovascular-related death. We investigated changes in circulating exosomal microRNA (miRNA) profiles in patients with DM with obesity compared with patients without DM with obesity.Research design and methodsThis prospective study involved 29 patients with obesity (patients without DM=16, patients with DM=13) and healthy volunteers (HVs) (n=18). We measured circulating levels of exosomal miRNAs by next-generation sequencing and compared miRNA levels across the three groups.ResultsThe expression levels of 25 miRNAs (upregulated=14, downregulated=11) differed between patients with obesity with DM and patients with obesity without DM. Compared with HV, patients with DM with obesity had 53 dysregulated miRNAs. Additionally, moving stepwise from HV to patients with obesity without DM to patients with obesity with DM, there was a consistent increase in expression levels of miR-23a-5p and miR-6087 and a consistent decrease in expressions levels of miR-6751-3p.ConclusionsOur data show that the exosomal miRNAs is altered by dysregulated glucose metabolism in patients with obesity. This circulating exosomal miRNA signature in patients with obesity with or without DM is a potential biomarker and therapeutic target in these patients.

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Diagnosis of Hyperacute and Acute Ischaemic Stroke: The Potential Utility of Exosomal MicroRNA-21-5p and MicroRNA-30a-5p

Cerebrovascular Diseases ◽

10.1159/000488365 ◽

2018 ◽

Vol 45 (5-6) ◽

pp. 204-212 ◽

Cited By ~ 14

Author(s):

Wei Wang ◽

Dong-Bin Li ◽

Ru-Ying Li ◽

Xia Zhou ◽

Dong-Ju Yu ◽

...

Keyword(s):

Ischaemic Stroke ◽

Early Stage ◽

Area Under The Curve ◽

Recovery Phase ◽

Accurate Diagnosis ◽

Chain Reaction ◽

Expression Levels ◽

Transmission Electron ◽

Polymerase Chain ◽

Exosomal Microrna

Background: Early and accurate diagnosis of ischaemic stroke (IS) requires the use of an optimized biomarker. Exosomal microRNAs have the potential to serve as biomarkers owing to their stability and specificity. We investigated the expression levels of plasma-derived exosomal microRNA-21-5p and microRNA-30a-5p in the different phases of IS. Methods: One hundred forty-three patients with IS and 24 non-stroke controls were enrolled. The patients were divided into the following 5 groups: 1 group for the hyperacute phase IS (HIS, within 6 h); two for the acute phase IS (AIS, including days 1–3 and days 4–7); one for the subacute phase IS (SIS, days 8–14); and one for the recovery phase IS (RIS, days >14). Plasma exosomes were isolated using a QIAGEN exoRNeasy kit and examined by transmission electron microscopy, nanoparticle tracking, and flow cytometry. The expression levels of miRNA-21-5p and miRNA-30a-5p were detected by quantitative real-time polymerase chain reaction. Results: The plasma exosomal miR-21-5p levels in SIS and RIS were significantly higher than that in controls (p < 0.05 and p < 0.01 respectively). The levels of miR-30a-5p in HIS were significantly higher (p < 0.05) and in AIS (days 1–3) were lower than that in controls (p < 0.05). In AIS (days 1–3), both miRNAs were decreased compared with the HIS group (p = 0.053 and 0.001, respectively). The area under the curve (AUC) of the miR-21-5p was 0.714 for SIS (95% CI 0.570–0.859, p = 0.007), 0.734 for RIS (95% CI 0.596–0.871, p = 0.003); the AUC of the miR-30a-5p was 0.826 for HIS (95% CI 0.665–0.988, p = 0.001), 0.438 for AIS (days 1–3; 95% CI 0.240–0.635, p = 0.516). Conclusions: The plasma-derived exosomal miR-21-5p and miRNA-30a-5p in combination are promising biomarkers for diagnosing IS and distinguishing among HIS, SIS, and RIS, especially miRNA-30a-5p for the diagnosis of the HIS phase. Our results provide a new reference for clinicians to apply in early-stage diagnosis and identifies the possible value of biomarkers for IS thrombolysis therapy.

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Investigation of SPARC protein expression levels at dopaminergic system in mouse brain treated with repeated morphine administration

Neuroscience Research ◽

10.1016/s0168-0102(00)81595-3 ◽

2000 ◽

Vol 38 ◽

pp. S125

Author(s):

S Akiduki

Keyword(s):

Protein Expression ◽

Mouse Brain ◽

Dopaminergic System ◽

Expression Levels ◽

Morphine Administration

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Gene expression analysis of metabolic markers during bone regeneration in a rat model

Osteologie/Osteology ◽

10.1055/s-0037-1620051 ◽

2014 ◽

Vol 23 (03) ◽

pp. 207-211

Author(s):

C. Kasch ◽

A. Osterberg ◽

Thordis Granitzka ◽

T. Lindner ◽

M. Haenle ◽

...

Keyword(s):

Gene Expression ◽

Gene Expression Analysis ◽

Saline Solution ◽

Female Rats ◽

Synthesis Rate ◽

Metabolic Markers ◽

Expression Levels ◽

Fibrotic Tissue ◽

Intermittent Pth ◽

Lower Expression

SummaryThe RANK/RANKL/OPG system plays an important role in the regulation of bone metabolism and bony integration around implants. The aim of this study was to analyse gene expression of OPG, RANK, and RANKL in regenerating bone during implant integration. Additionally, the effect of intermittent para - thyroid hormone (PTH) treatment was analysed. A titanium chamber was implanted in the proximal tibiae of 48 female rats. The animals received either human PTH or saline solution (NaCl). After 21 and 42 days, RNA was isolated from tissue adjacent to the implant and expression of RANK, RANKL, and OPG was analysed. After 21 days, very low expression levels of all genes were shown. In contrast, increased gene expression after 42 days was determined. Expression of RANK and RANKL was lower than that for OPG. The lower expression levels after 21 days might be due to still ossifying, fibrotic tissue around the titanium chamber. An increased OPG synthesis rate associated with decreased RANKL expression after 42 days revealed bone-forming processes. Despite significant differences in gene expression between the time points, only slight differences were observed between application of intermittent PTH and NaCl after a period of 42 days.

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High Level Expression of Active Human Prothrombin in a Vaccina Virus Expression System

Thrombosis and Haemostasis ◽

10.1055/s-0038-1656335 ◽

1992 ◽

Vol 68 (02) ◽

pp. 119-124 ◽

Cited By ~ 10

Author(s):

F G Falkner ◽

P L Turecek ◽

R T A MacGillivray ◽

W Bodemer ◽

F Scheiflinger ◽

...

Keyword(s):

Vaccinia Virus ◽

Cell Lines ◽

Expression System ◽

Human Cell Line ◽

Cell System ◽

Time Saving ◽

Expression Levels ◽

Mammalian Cell Lines ◽

Cell Line Hela ◽

Virus Expression

SummaryWe have worked out an efficient and time saving procedure for the expression of recombinant human prothrombin. The glycoprotein was expressed in the vaccinia virus expression system in several mammalian cell lines. The kidney cell lines Vero and BHK and the human cell line Hela were found to efficiently secrete prothrombin. Expression levels of 3–4 µg of factor II per 106 cells per day corresponding to 18–23 mU per 106 cells per day were achieved. Since the expression levels obtained with the vaccinia virus/Vero cell system were comparable to those obtained in amplified transformed CHO cells it provides an alternative system for the efficient expression of human prothrombin and may allow to further elucidate structure-function relationships of (pro)thrombin and its various effectors.

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Gene expression levels of Casein kinase 1 (CK1) isoforms are correlated to adiponectin levels in adipose tissue and site-specific phosphorylation mediated by CK1 influences multimerization of adiponectin

Zeitschrift für Gastroenterologie ◽

10.1055/s-0038-1669041 ◽

2018 ◽

Vol 56 (08) ◽

pp. e345-e346

Author(s):

P Xu ◽

P Fischer-Posovszky ◽

M Wabitsch ◽

U Knippschild

Keyword(s):

Gene Expression ◽

Adipose Tissue ◽

Casein Kinase ◽

Casein Kinase 1 ◽

Site Specific ◽

Expression Levels ◽

Gene Expression Levels

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Dynamics of fatty acid regulatory genes during ovarian development in Penaeus monodon

Reproduction ◽

10.1530/rep-18-0143 ◽

2018 ◽

Cited By ~ 1

Author(s):

Pacharawan Deenarn ◽

Punsa Tobwor ◽

Rungnapa Leelatanawit ◽

Somjai Wongtriphop ◽

Jutatip Khudet ◽

...

Keyword(s):

Fatty Acid ◽

Ovarian Development ◽

Penaeus Monodon ◽

Chain Fatty Acid ◽

Regulatory Genes ◽

Ovarian Maturation ◽

Long Chain Fatty Acid ◽

Eyestalk Ablation ◽

Regulatory Pathways ◽

Expression Levels

The delay in ovarian maturation in farmed black tiger shrimp Penaeus monodon has resulted in the widespread practice of feeding broodstock with the polychaetes Perinereis nuntia and their unilateral eyestalk ablation. Although this practice alters fatty acid content in shrimp ovaries and hepatopancreas, its effects on fatty acid regulatory genes have yet to be systematically examined. Here, microarray analysis was performed on hepatopancreas and ovary cDNA collected from P. monodon at different ovarian maturation stages, revealing that 72 and 58 genes in fatty acid regulatory pathways were differentially expressed in hepatopancreas and ovaries respectively. Quantitative real-time PCR analysis revealed that ovarian maturation was associated with higher expression levels of acetyl-CoA acetyltransferase, acyl-CoA dehydrogenase, acyl-CoA oxidase 3 and long-chain fatty acid transport protein 4 in hepatopancreas, whereas the expression levels of 15 fatty acid regulatory genes were increased in shrimp ovaries. To distinguish the effects of different treatments, transcriptional changes were examined in P. monodon with stage 1 ovaries before polychaete feeding, after one-month of polychaete feeding and after eyestalk ablation. Polychaete feeding resulted in lower expression levels of enoyl-CoA hydratase and acyl-CoA synthetase medium-chain family member 4, while the expression level of phosphatidylinositide phosphatase SAC1 was higher in shrimp hepatopancreas and ovaries. Additionally, eyestalk ablation resulted in a higher expression level of long-chain fatty acid-CoA ligase 4 in both tissues. Together, our findings describe the dynamics of fatty acid regulatory pathways during crustacean ovarian development and provide potential target genes for alternatives to eyestalk ablation in the future.

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