Elevated Serum Concentrations of Polymorphonuclear Neutrophilic Leukocyte Elastase in Systemic Sclerosis: Association with Pulmonary Fibrosis

2009 ◽  
Vol 36 (1) ◽  
pp. 99-105 ◽  
Author(s):  
TOSHIHIDE HARA ◽  
FUMIHIDE OGAWA ◽  
KOICHI YANABA ◽  
YOHEI IWATA ◽  
EIJI MUROI ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Pulmonary Fibrosis ◽  
Elevated Serum ◽  
Serum Levels ◽  
Oxidative Stress Marker ◽  
Surfactant Protein D ◽  
Serum Concentrations ◽  
Leukocyte Elastase ◽  
Pmn Elastase ◽  
Pmn Élastase

ObjectiveTo determine the serum concentrations and clinical association of polymorphonuclear neutrophilic leukocyte (PMN) elastase in patients with systemic sclerosis (SSc).MethodsSerum PMN elastase levels from 21 patients with limited cutaneous SSc (lSSc) and 32 with diffuse cutaneous SSc (dSSc) were examined by ELISA.ResultsSerum PMN elastase levels were elevated in patients with SSc, especially dSSc, compared to healthy controls. SSc patients with elevated serum PMN elastase levels had more frequent presence of pulmonary fibrosis, arthritis, contracture of phalanges, and diffuse pigmentation. Anticentromere antibody was detected less frequently in SSc patients with elevated serum PMN elastase levels than in controls. Consistently, serum PMN elastase levels also correlated positively with serum levels of KL-6 and surfactant protein-D, serological markers for pulmonary fibrosis. Serum PMN elastase levels were also associated with levels of serum 8-isoprostane, an oxidative stress marker in SSc.ConclusionSerum PMN elastase levels were elevated in patients with SSc, and it was more prominent in patients with pulmonary fibrosis, suggesting that serum PMN elastase is a novel serological marker for SSc-related pulmonary fibrosis.

Use of Serum Clara Cell 16-kDa (CC16) Levels as a Potential Indicator of Active Pulmonary Fibrosis in Systemic Sclerosis

2011 ◽  
Vol 38 (5) ◽  
pp. 877-884 ◽  
Author(s):  
MINORU HASEGAWA ◽  
MANABU FUJIMOTO ◽  
YASUHITO HAMAGUCHI ◽  
TAKASHI MATSUSHITA ◽  
KATSUMI INOUE ◽  
...  
Keyword(s):  
Longitudinal Study ◽  
Systemic Sclerosis ◽  
Pulmonary Fibrosis ◽  
Lupus Erythematosus ◽  
Characteristic Curve ◽  
Serum Levels ◽  
Clara Cell ◽  
Inactive Disease ◽  
Surfactant Protein D ◽  
Healthy Controls

Objective.To clarify the clinical significance of concentrations of serum Clara cell 16-kDa protein (CC16; previously denoted CC10) in the diagnosis and monitoring of pulmonary fibrosis (PF) in patients with systemic sclerosis (SSc); and to compare CC16 levels with levels of the current most reliable serum markers for PF, such as Krebs von den Lungen-6 (KL-6) antigen and surfactant protein-D (SP-D).Methods.Serum levels of CC16, KL-6, and SP-D were determined by ELISA in 92 patients with SSc, 20 patients with systemic lupus erythematosus (SLE), and 20 healthy controls. In a retrospective longitudinal study, correlation of serum CC16 levels with the activity of PF was assessed in 16 SSc patients with PF.Results.Although CC16 levels were higher in patients with SSc than in SLE patients or healthy controls, the difference was not significant. Increased serum CC16 levels were associated with involvement of PF, especially active PF, as well as KL-6 and SP-D. Receiver operating characteristic curve analysis revealed that the utility of CC16 is slightly inferior to KL-6, but was comparable with that of SP-D for detecting PF in patients with SSc. In the longitudinal study, serum levels of CC16, KL-6, and SP-D were significantly decreased in the inactive disease phase compared to the active disease phase.Conclusion.CC16 levels can be used as a potential serum biomarker for PF in addition to KL-6 and SP-D in patients with SSc.

Elevated Serum Concentrations of Triggering Receptor Expressed on Myeloid Cells-1 in Diffuse Cutaneous Systemic Sclerosis: Association with Severity of Pulmonary Fibrosis

2010 ◽  
Vol 37 (4) ◽  
pp. 787-791 ◽  
Author(s):  
HAJIME TOMITA ◽  
FUMIHIDE OGAWA ◽  
TOSHIHIDE HARA ◽  
KOICHI YANABA ◽  
YOHEI IWATA ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Pulmonary Fibrosis ◽  
Vital Capacity ◽  
Normal Values ◽  
Elevated Serum ◽  
Myeloid Cells ◽  
Serum Concentrations ◽  
Diffusion Capacity ◽  
Healthy Control ◽  
Diffuse Cutaneous Systemic Sclerosis

Objective.To determine serum concentrations and clinical association of soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) in patients with systemic sclerosis (SSc).Methods.Serum sTREM-1 levels from 17 patients with limited cutaneous SSc (lSSc), 24 patients with diffuse cutaneous SSc (dSSc), and 29 healthy control individuals were examined by ELISA.Results.Total SSc patients exhibited significantly elevated serum sTREM-1 levels relative to controls (p < 0.01). Serum sTREM-1 levels were significantly elevated in patients with dSSc compared to controls (p < 0.005) and lSSc patients (p < 0.05). By contrast, sTREM-1 levels in lSSc were similar to those in controls. Serum sTREM-1 levels were significantly elevated in SSc patients with decreased percentage vital capacity (%VC). Consistent with this, serum sTREM-1 levels in SSc patients correlated negatively with %VC (r = −0.24, p < 0.005). Among SSc patients with pulmonary fibrosis, sTREM-1 levels were significantly increased in patients with decreased %VC or decreased percentage of diffusion capacity for carbon monoxide relative to those with normal values (p < 0.05).Conclusion.Serum sTREM-1 levels were elevated in dSSc patients and correlated with severity of pulmonary fibrosis, suggesting that serum sTREM-1 is a novel serological marker for the disease severity of SSc.

scholarly journals Elevated Serum Levels of Arachidonoyl-lysophosphatidic Acid and Sphingosine 1-Phosphate in Systemic Sclerosis

2009 ◽  
pp. 168-176 ◽  
Author(s):  
Akira Tokumura ◽  
Laura D. Carbone ◽  
Yasuko Yoshioka ◽  
Junichi Morishige ◽  
Masaki Kikuchi ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Lysophosphatidic Acid ◽  
Elevated Serum ◽  
Serum Levels ◽  
Sphingosine 1 Phosphate

scholarly journals The overexpression of peroxiredoxin-4 affects the progression of idiopathic pulmonary fibrosis

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Tetsuya Hanaka ◽  
Takashi Kido ◽  
Shingo Noguchi ◽  
Sohsuke Yamada ◽  
Hirotsugu Noguchi ◽  
...  
Keyword(s):  
Idiopathic Pulmonary Fibrosis ◽  
Pulmonary Fibrosis ◽  
Healthy Volunteers ◽  
Area Under The Curve ◽  
Serum Levels ◽  
Lung Epithelial Cells ◽  
Enzyme Linked Immunosorbent Assay ◽  
Surfactant Protein D ◽  
Intratracheal Administration ◽  
Inflammatory Changes

Abstract Background Acute exacerbation of idiopathic pulmonary fibrosis (AE-IPF) is life-threatening. Several serum biomarkers, such as Krebs von den Lungen-6 (KL-6) and surfactant protein D (SP-D), are clinically used for evaluating AE-IPF, but these biomarkers are not adequate for establishing an early and accurate diagnosis of AE-IPF. Recently, the protective roles of the members of the peroxiredoxin (PRDX) family have been reported in IPF; however, the role of PRDX4 in AE-IPF is unclear. Methods Serum levels of PRDX4 protein, KL-6, SP-D and lactate dehydrogenase (LDH) in 51 patients with stable IPF (S-IPF), 38 patients with AE-IPF and 15 healthy volunteers were retrospectively assessed using enzyme-linked immunosorbent assay. Moreover, as an animal model of pulmonary fibrosis, wild-type (WT) and PRDX4-transgenic (Tg) mice were intratracheally administered with bleomycin (BLM, 2 mg/kg), and fibrotic and inflammatory changes in lungs were evaluated 3 weeks after the intratracheal administration. Results Serum levels of PRDX4 protein, KL-6, SP-D and LDH in patients with S-IPF and AE-IPF were significantly higher than those in healthy volunteers, and those in AE-IPF patients were the highest among the three groups. Using receiver operating characteristic curves, area under the curve values of serum PRDX4 protein, KL-6, SP-D, and LDH for detecting AE-IPF were 0.873, 0.698, 0.675, and 0.906, respectively. BLM-treated Tg mice demonstrated aggravated histopathological findings and poor prognosis compared with BLM-treated WT mice. Moreover, PRDX4 expression was observed in alveolar macrophages and lung epithelial cells of BLM-treated Tg mice. Conclusions PRDX4 is associated with the aggravation of inflammatory changes and fibrosis in the pathogenesis of IPF, and serum PRDX4 may be useful in clinical practice of IPF patients.

Gastro-intestinal polypeptides in patients treated for medullary carcinoma of the thyroid

1984 ◽  
Vol 106 (1) ◽  
pp. 112-115 ◽  
Author(s):  
Bente Rasmusson
Keyword(s):  
Serum Gastrin ◽  
Clinical Course ◽  
Elevated Serum ◽  
Medullary Carcinoma ◽  
Serum Levels ◽  
High Serum ◽  
Serum Concentrations ◽  
Serum Calcitonin ◽  
Basal Serum ◽  
Previously Treated

Abstract. In 12 patients treated 2 to 58 months previously for medullary carcinoma of the thyroid, basal serum concentrations of calcitonin, gastrin, vasoactive intestinal polypeptide, glucagon, insulin, and pancreatic polypeptide were measured in search of any correlation between these and the clinical course of the disease. All patients had elevated serum calcitonin levels indicating present disease. One patient had increased serum concentrations of several hormones. Another had achlorhydria and high serum gastrin levels. No relationship between calcitonin and gastro-intestinal polypeptides was found in 11 patients. No correlations were found between serum levels of polypeptides and the occurrence of diarrhoea in 5 patients. It is concluded that gastro-intestinal polypeptides, which are produced by other apudomas, are not secreted in more than normal concentrations under basal conditions, by the majority of patients previously treated for medullary carcinoma of the thyroid.

AB0057 Increased IL-35 Serum Levels in Systemic Sclerosis Indicates Association with Pulmonary Fibrosis

2015 ◽  
Vol 74 (Suppl 2) ◽  
pp. 909.2-909
Author(s):  
A.T. Dantas ◽  
S.M.C. Gonçalves ◽  
M.C. Pereira ◽  
R.S.G. Gonçalves ◽  
C.D.L. Marques ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Pulmonary Fibrosis ◽  
Serum Levels

Elevated serum levels of interleukin-1β and interleukin-33 in patients with systemic sclerosis in Chinese population

2016 ◽  
Vol 77 (2) ◽  
pp. 151-159 ◽  
Author(s):  
Y.-J. Zhang ◽  
Q. Zhang ◽  
G.-J. Yang ◽  
J.-H. Tao ◽  
G.-C. Wu ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Chinese Population ◽  
Elevated Serum ◽  
Serum Levels ◽  
Interleukin 1Β ◽  
Interleukin 33

scholarly journals Concentrations of Mg, Ca, Fe, Cu, Zn, P and anthropometric and biochemical parameters in adults with chronic heart failure

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12207
Author(s):  
Iwona Gorący ◽  
Ewa Rębacz-Maron ◽  
Jan Korbecki ◽  
Jarosław Gorący
Keyword(s):  
Heart Failure ◽  
Total Cholesterol ◽  
Biochemical Parameters ◽  
Elevated Serum ◽  
Serum Levels ◽  
High Density ◽  
Emission Spectrometry ◽  
Low Density ◽  
Serum Concentrations ◽  
Presenting Symptoms

Background The study investigated the relationship between the concentrations of Mg, Ca, Fe, Cu, Zn, P and anthropometric and biochemical parameters in the blood serum of patients with heart failure (HF) and the potential influence on the development and progression of HF. Material & methods The study included 214 patients (155 men and 59 women), aged 40–87 years, presenting symptoms or signs typical of HF (according to the NYHA functional classification). Serum concentrations were determined for Mg, Ca, Fe, Cu, Zn, P, C-reactive protein (CRP), creatinine, urea, triglyceride levels (TG), total cholesterol (CH), high density protein (HDL), low density protein (LDL). The levels of macro-and microminerals were analysed using inductively coupled serum optical emission spectrometry (ICP-OES). Results Our study confirmed the role of known risk factors in the development of heart failure, including: overweight, diabetes, hypertension, high triglycerides (TG), high total cholesterol (CH), high levels of low density protein (LDL) and reduced levels of high density protein (HDL), high CRP, high creatinine. Moreover, deficient serum concentrations of Mg (47% of the studied men and 54% of the women) and Cu (in 44% of men and more than 30% of women) were observed, as well as subnormal serum Fe (2% of women) and Zn (1% of men). Elevated serum Ca was found in 50% of men and 49% of women. In 44% of the studied men and 52% of the studied women, P levels in serum were also above-average. The study revealed a significant positive correlation between serum levels of Ca and Mg, and also Ca and Cu in women. In men, serum Cu was positively correlated with Mg and Ca concentrations. In patients from group 1 (NYHA I–II), Mg content was positively correlated with Ca and Cu. In this patient group, Ca was also positively associated with Cu content in serum. In group 2 (NYHA III-IV), serum Mg concentration was significantly positively correlated with that of Cu and Ca. Conclusions Changes in the serum concentrations of macro-and microminerals may significantly affect the severity of HF in Polish patients.

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