Effect of Saccharomyces cerevisiae addition to feed contaminated with aflatoxin B1 on the health and performance indices of tambaqui (Colossoma macropomum) fingerlings

2021 ◽  
pp. 1-12
Author(s):  
R.E.E. Pinheiro ◽  
A.M.D. Rodrigues ◽  
E.K.F. Batista ◽  
A.M. Monte ◽  
M.N. Ribeiro ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Experimental Unit ◽  
Histopathological Analysis ◽  
Performance Indices ◽  
Initial Weight ◽  
Randomised Study ◽  
Liver And Kidney ◽  
Final Length ◽  
And Performance ◽  
Aflatoxin B

The objective of this study was to evaluate the effects of the addition of Saccharomyces cerevisiae to feed contaminated with aflatoxin B1 (AFB1) on the performance and health of tambaqui fingerlings. A fully randomised study design was used, represented by four treatments: (T1) with 0.0 μg/kg of AFB1 and 0.0 colony forming unit (cfu)/g of yeast (control); (T2) with 0.0 μg/kg of AFB1 and 106 cfu/g of yeast; (T3) with 100 μg/kg of AFB1 and 0.0 cfu/g of yeast and (T4) with 100 μg/kg of AFB1 and 106 cfu/g of yeast, with four repetitions per treatment. Over a period of 34 days, 192 fingerlings with an initial weight of 2.9±0.2 g and length of 26.1±1.3 mm were used, distributed in 16 tanks with 12 fish per experimental unit. The following parameters were evaluated: water quality, zootechnical performance, histopathological analysis, counting and isolation of yeasts from the feed and intestine. No significant differences were observed (P>0.05) in the initial weight, initial length, final length, specific growth rate, mean length gain, Fulton condition factor (K Factor) or survival rate of the tambaqui fingerlings during the trial period. Statistical differences (P<0.05) were found between the treatments with respect to fingerling final weight, mean weight gain and average feed intake. Histopathological changes in the liver and kidney tissues from tambaqui fingerlings were more pronounced in T3 and T4. Based on histopathological findings, the yeast was unable to reduce the characteristic effects of aflatoxicosis in tambaqui fingerlings fed with 100 μg/kg AFB1. The S. cerevisiae A8L2 strain was able to colonise the intestine of tambaqui fingerlings, however, its addition in conjunction with 100 μg/kg AFB1 to fingerling feed did not promote improvements in performance indices or the histopathological parameters of these animals.

scholarly journals Digestible threonine-to-lysine ratio for piglets under health challenge

2020 ◽  
Vol 9 (12) ◽  
pp. e21691210899
Author(s):  
Gregório Murilo de Oliveira Júnior ◽  
Valéria Vânia Rodrigues ◽  
Priscila Furtado Campos ◽  
Andressa da Silva Formigoni ◽  
Wilams Gomes dos Santos ◽  
...  
Keyword(s):  
Block Design ◽  
Experimental Unit ◽  
Serum Urea ◽  
Weaned Piglets ◽  
Initial Weight ◽  
Quadratic Effect ◽  
Randomized Block Design ◽  
Carcass Parameters ◽  
Liver And Kidney ◽  
Plasma Haptoglobin

Aiming to determine the ration between digestible lysine and digestible threonine (Thr/Lys) for weaned piglets at 27 days, kept in the nursery under sanitary challenge conditions, 105 hybrid piglets with initial weight of 7.2 ± 0.41 kg, were distributed in randomized block design inside of five treatments (Thr/Lys ratios of 0.56; 0.63; 0.70; 0.77 and 0.84), seven replicates and three piglets per experimental unit. The Thr/Lys ratios did not affect (P>0.05) piglet performance. There was quadratic effect (P=0.02) of the Thr/Lys ratio on the protein/fat ratio and the estimated point of the bigger protein/fat ratio occurred with the Thr/Lys ratio of 0.68. The Thr/Lys ratio did not affect (P>0.05) the carcass parameters and serum urea levels, however, the liver and kidney weights raised with the increase (P=0.01) in Thr/Lys rate. The Thr/Lys ratio had a quadratic effect (P=0.01) on rate of plasma haptoglobin, Thr/Lys ratio was estimated at 0.74. It was concluded that the ratio between digestible threonine with digestible lysine for piglets from 27 to 48 days of age raised under sanitary challenge conditions is 0.74.

Preparation and Biochemical Evaluation of Functionalized Multi-Walled Carbon Nanotubes with Punica granatum Extract

2019 ◽  
Vol 15 (1) ◽  
pp. 138-144 ◽  
Author(s):  
Ahmed A. Haroun ◽  
Abdel-Tawab H. Mossa ◽  
Samia M.M. Mohafrash
Keyword(s):  
Liver Enzymes ◽  
Histopathological Analysis ◽  
Pomegranate Peel ◽  
Liver And Kidney ◽  
Multi Walled Carbon Nanotubes ◽  
Pomegranate Peel Extract ◽  
Walled Carbon Nanotubes ◽  
Functionalized Mwcnts ◽  
Peel Extract

Background: Funcionalized multi-walled carbon nanotubes (ox-MWCNTs) were used for the preparation of therapeutic nanoparticles for delivery of some bioactive compounds. Consequently, this work deals with the preparation of grafted MWCNTs with n-vinyl caprolactam in the presence of pomegranate peel extract (P. granatum), titanium dioxide (TiO2) and/or silver nanoparticeles and their toxic effects on male mice using in vivo biological examination (liver and kidney dysfunction biomarkers) and the histopathological analysis. Methods: P. granatum extract was immobilized onto functionalized MWCNTs using simple adsorption technique. Moreover, The prepared materials were analyzed by Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM). In vivo examination using liver and kidney dysfunction biomarkers was investigated. In addition, the histopathological study was carried out. Results: The ox-MWCNTs induced significant elevation in the liver enzymes including AST, ALT and ALP relative to the control group. While, the treatment with P. granatum extract only did not induce any change in the liver and kidney biomarkers. In other words, P. granatum extract loaded onto functionalized MWCNTs showed low effects on liver enzymes and kidney function biomarkers in the treated mice in comparison with ox-MWCNTs and extract separately. Moreover, histopathological analysis revealed that the P. granatum extract functionalized MWCNTs exhibited normal renal tissue with no histopathological alteration. Conclusion: The grafted MWCNTs with n-vinyl caprolactam in the presence of pomegranate peel extract (P. granatum), titanium dioxide (TiO2) and/or silver nanoparticeles were successfully prepared. SEM-micrographs showed complete coating of MWCNTs fiber with the extract. The prepared materials resulted in no toxic effects and the histopathological findings were confirmed by inflammation of the liver and kidney tissues.

scholarly journals PSXII-17 Trace minerals source influences the performance of Nellore cattle in the growing phase during the transition between dry to rainy season in Brazil

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 445-446
Author(s):  
Iorrano A Cidrini ◽  
Josiane F Lage ◽  
Igor Ferreira ◽  
Karla Oliveira ◽  
Laura F Prados ◽  
...  
Keyword(s):  
Rainy Season ◽  
Relative Bioavailability ◽  
Trace Minerals ◽  
Experimental Unit ◽  
Liver Concentration ◽  
Nellore Cattle ◽  
And Performance ◽  
Urochloa Brizantha ◽  
Cu And Zn ◽  
Matter Basis

Abstract The relative bioavailability of trace minerals may vary between the sources and impact the animal performance. The study was carried out to evaluate the effect of TM source (Cu and Zn) on the liver concentration and performance of Nellore cattle supplemented in the growing phase during the transition between dry to rainy season. A hundred and twenty Nellore young bulls (BW = 349.72 ± 24 kg; 24 months), blocked by BW, were assigned randomly to twelve paddocks (six paddocks/treatment and 10 animals/paddock). Animals grazing Urochloa brizantha cv. Marandu and supplemented at 5 g/kg BW with soybean meal, corn and mineral mix (250 g/kg of CP and 650 g/kg de NDT, natural matter basis). Supplements consisted of 40 mg Cu and 148 mg Zn/kg DM from either inorganic TM (ITM) or hydroxy TM (HTM) Intellibond® sources. Liver samples were collected at the beginning and end of the study (three animals per paddock). Animals were weighed at each period (30 days) during three periods. Period was used as a REPEATED measurement for BW and values of initial BW as independent covariates for all variables. Paddock was considered experimental unit. Data were analyzed by ANOVA using PROC MIXED, SAS 9.4. The animals supplemented with HTM had higher (+0.037 kg/day) general ADG (day 0 to 90; P = 0.012), with 0.469 and 0.506 kg/day to ITM and HTM, respectively. Final BW (day 90) from animals fed HTM was 5.13 kg higher than ITM (391.97 vs. 397.11 kg BW; P = 0.030). No differences were found in the liver concentrations of Cu and Zn between ITM or HTM (P ≥ 0.288). Cu and Zn from HTM improve the performance of animals supplemented in pasture compared to ITM sources.

scholarly journals Sectorization for Water Distribution Systems with Multiple Sources: A Performance Indices Comparison

Water ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 131
Author(s):  
Jezabel D. Bianchotti ◽  
Melina Denardi ◽  
Mario Castro-Gama ◽  
Gabriel D. Puccini
Keyword(s):  
Distribution Systems ◽  
Water Distribution ◽  
Distribution Networks ◽  
Performance Criteria ◽  
Performance Indices ◽  
Multiple Sources ◽  
Multiobjective Problems ◽  
Open Questions ◽  
Minimum Number ◽  
And Performance

Sectorization is an effective technique for reducing the complexities of analyzing and managing of water systems. The resulting sectors, called district metering areas (DMAs), are expected to meet some requirements and performance criteria such as minimum number of intervention, pressure uniformity, similarity of demands, water quality and number of districts. An efficient methodology to achieve all these requirements together and the proper choice of a criteria governing the sectorization is one of the open questions about optimal DMAs design. This question is addressed in this research by highlighting the advantages of three different criteria when applied to real-word water distribution networks (WDNs). To this, here it is presented a two-stage approach for optimal design of DMAs. The first stage, the clustering of the system, is based on a Louvain-type greedy algorithm for the generalized modularity maximization. The second stage, the physical dividing of the system, is stated as a two-objective optimization problem that utilises the SMOSA version of simulated annealing for multiobjective problems. One objective is the number of isolation valves whereas for the second objective three different performance indices (PIs) are analyzed and compared: (a) standard deviation, (b) Gini coefficient and (c) loss of resilience. The methodology is applied to two real case studies where the first two PIs are optimized to address similar demands among DMAs. The results demonstrate that the proposed method is effective for sectorization into independent DMAs with similar demands. Surprisingly, it found that for the real studied systems, loss of resilience achieves better performance for each district in terms of pressure uniformity and demand similarity than the other two specific performance criteria.

scholarly journals Evaluation of the Coating with TiO2 Nanoparticles as an Option for the Improvement of the Characteristics of NiTi Archwires: Histopathological, Cytotoxic, and Genotoxic Evidence

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Javier Morán-Martínez ◽  
Roberto Beltrán del Río-Parra ◽  
Nadia Denys Betancourt-Martínez ◽  
Rubén García-Garza ◽  
Joel Jiménez-Villarreal ◽  
...  
Keyword(s):  
Cell Viability ◽  
Blood Sample ◽  
Tio2 Nanoparticles ◽  
Niti Alloy ◽  
Renal Tissue ◽  
Male Rats ◽  
Histopathological Analysis ◽  
Liver And Kidney ◽  
The Times ◽  
Nuclear Alterations

For the EPD, different voltages and different times were used. Male rats were used in four groups (n=3) with different treatments. The blood sample was obtained for genotoxic analysis and liver and kidney organs were removed for histopathological analysis. The amount of NPs TiO2 deposited on the samples of the arches increases gradually in the times of 15 and 30 s. At all voltages, however, at 45, 60, 75, and 90 s, there is an increase up to 25 V. Cell viability in lymphocytes treated with TiO2 NPs did not cause genotoxicity. In the histopathological findings of hepatic and renal tissue, nuclear alterations and necrosis were observed. The objective of the study was to improve the physical and biocompatibility characteristics of the NiTi arches for which the EPD is used. The technique for the deposition of TiO2 NPs was used, where this technique could be used as an economical and versatile way to perform homogeneous depositions even on surfaces with the complexity of the NiTi alloy. As for genotoxicity and cytotoxicity, we continue to have controversial results.

scholarly journals Reference evapotranspiration for Londrina, Paraná, Brazil: performance of different estimation methods

2017 ◽  
Vol 38 (4Supl1) ◽  
pp. 2363
Author(s):  
Rodrigo Dlugosz da Silva ◽  
Marcelo Augusto de Aguiar e Silva ◽  
Marcelo Giovanetti Canteri ◽  
Juliandra Rodrigues Rosisca ◽  
Nilson Aparecido Vieira Junior
Keyword(s):  
Reference Evapotranspiration ◽  
Meteorological Data ◽  
Global Radiation ◽  
Alternative Methods ◽  
Estimation Methods ◽  
Performance Indices ◽  
Excellent Performance ◽  
Study Region ◽  
Temperature Radiation ◽  
And Performance

Aiming at assessing the performance of alternative methods to Penman-Monteith FAO56 for estimating the reference evapotranspiration (ETo) for Londrina, Paraná, Brazil, the methods temperature radiation, Hicks-Hess, Hargreaves-Samani (1982), Turc, Priestley-Taylor, Tanner-Pelton, Jensen-Haise, Makkink, modified Hargreaves, Stephens-Stewart, Abtew, global radiation, Ivanov, Lungeon, Hargreaves-Samani (1985), Benavides-Lopez, original Penman, Linacre, Blaney-Morin, Romanenko, Hargreaves (1974), McCloud, Camargo, Hamon, Kharrufa, McGuiness-Bordne, and Blaney-Criddle were compared to that standard method recommended by FAO. The estimations were correlated by linear regression and assessed by using the Person’s correlation coefficient (r), concordance index (d), and performance index (c) using a set of meteorological data of approximately 40 years. The methods modified Hargreaves, Stephens-Stewart, Abtew, global radiation, Ivanov, Lungeon, Hargreaves-Samani (1985), Benavides-Lopez, original Penman, and Linacre should be avoided, as they did not present excellent results. The methods McCloud, Camargo, Hamon, Kharrufa, McGuinness-Bordne, Blaney-Criddle, Hargreaves (1974), Romanenko, and Blaney-Morin were classified as very bad, not being recommended. In contrast, the methods temperature radiation, Hicks-Hess, Hargreaves-Samani (1982), Turc, Priestley-Taylor, Tenner-Pelton, Jensen-Haise, and Makkink presented excellent performance indices and can be applied in the study region.

scholarly journals Evaluation of different corn textures in dry grain or silage forms for piglets from 7 to 15 kg

2009 ◽  
Vol 38 (9) ◽  
pp. 1757-1761
Author(s):  
José Vieira Neto ◽  
Vinícius de Souza Cantarelli ◽  
Elias Tadeu Fialho ◽  
Márcio Gilberto Zangeronimo ◽  
Nikolas de Oliveira Amaral ◽  
...  
Keyword(s):  
Dry Matter ◽  
Block Design ◽  
Metabolizable Energy ◽  
Experimental Unit ◽  
Feed Conversion ◽  
Initial Weight ◽  
Large White ◽  
Randomized Block Design ◽  
Independent Form ◽  
Ensilage Corn

The objective of this study was to evaluate different textures and storage forms of corn in piglet diets. Two experiments were conducted, one of which tested digestibility of nutrients and the other diet performance. In the digestibility trial, 24 crossbred (Landrace X Large White) barrows with an initial weight of 18.7 ± 1.5 kg were used. They were kept in metabolic cages in a randomized block design using the factorial arrangement 2 X 2 (type of corn - dent and flint X type of storage - moist grain silage and dry grain) and six replicates, with one animal as the experimental unit. The values of metabolizable energy (ME) obtained were 3841 kcal of ME/kg of dry matter (DM) from dry corn, 3912 kcal of ME/kg of DM from moist corn, 4022 kcal of DM from ensiled dry corn and 3928 kcal of ME/kg of DM from ensiled humidity corn. The ensilage process increases the digestibility coefficient of dry matter and crude protein and increases the digestible energy of the diets, independent of the process form. The type of corn did not influence these variables. In the performance trial, 60 barrows (initial weight of 6.5 ± 1.5 kg) of the same stock were used during 28 days of post-weaning distributed in the same experimental design, with five replicates and three animals as the experimental unit. The treatments did not influence the daily weigh gain or the daily feed intake. The dentate corn and the ensilage process decreased the feed conversion of the independent form (no significant interaction). The substitution of dry for ensilage corn increased the digestibility of the nutrients in the diets and the feed conversion of the piglets from 7 to 15 kg. Dentate corn promotes best feed conversion in this phase.

scholarly journals Reference evapotranspiration for Londrina, Paraná, Brazil: performance of different estimation methods

2017 ◽  
Vol 38 (4Supl1) ◽  
pp. 2363
Author(s):  
Rodrigo Dlugosz da Silva ◽  
Marcelo Augusto de Aguiar e Silva ◽  
Marcelo Giovanetti Canteri ◽  
Juliandra Rodrigues Rosisca ◽  
Nilson Aparecido Vieira Junior
Keyword(s):  
Reference Evapotranspiration ◽  
Meteorological Data ◽  
Global Radiation ◽  
Alternative Methods ◽  
Estimation Methods ◽  
Performance Indices ◽  
Excellent Performance ◽  
Study Region ◽  
Temperature Radiation ◽  
And Performance

Aiming at assessing the performance of alternative methods to Penman-Monteith FAO56 for estimating the reference evapotranspiration (ETo) for Londrina, Paraná, Brazil, the methods temperature radiation, Hicks-Hess, Hargreaves-Samani (1982), Turc, Priestley-Taylor, Tanner-Pelton, Jensen-Haise, Makkink, modified Hargreaves, Stephens-Stewart, Abtew, global radiation, Ivanov, Lungeon, Hargreaves-Samani (1985), Benavides-Lopez, original Penman, Linacre, Blaney-Morin, Romanenko, Hargreaves (1974), McCloud, Camargo, Hamon, Kharrufa, McGuiness-Bordne, and Blaney-Criddle were compared to that standard method recommended by FAO. The estimations were correlated by linear regression and assessed by using the Person’s correlation coefficient (r), concordance index (d), and performance index (c) using a set of meteorological data of approximately 40 years. The methods modified Hargreaves, Stephens-Stewart, Abtew, global radiation, Ivanov, Lungeon, Hargreaves-Samani (1985), Benavides-Lopez, original Penman, and Linacre should be avoided, as they did not present excellent results. The methods McCloud, Camargo, Hamon, Kharrufa, McGuinness-Bordne, Blaney-Criddle, Hargreaves (1974), Romanenko, and Blaney-Morin were classified as very bad, not being recommended. In contrast, the methods temperature radiation, Hicks-Hess, Hargreaves-Samani (1982), Turc, Priestley-Taylor, Tenner-Pelton, Jensen-Haise, and Makkink presented excellent performance indices and can be applied in the study region.

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