Effects of Thymoquinone and Iksan 526 callus Extract on B16F10 and A375 Cell Lines

2020 ◽  
Vol 16 (6) ◽  
pp. 479-491
Author(s):  
Seong-Hui Eo ◽  
Seon-Mi Yu ◽  
Yohan Han ◽  
Jung Han ◽  
Su Min Kim ◽  
...  
Keyword(s):  
Cell Lines ◽  
A375 Cell

scholarly journals Gene Instability-Related lncRNA Prognostic Model of Melanoma Patients via Machine Learning Strategy

2021 ◽  
Vol 2021 ◽  
pp. 1-22
Author(s):  
Kexin Yan ◽  
Yutao Wang ◽  
Yining Shao ◽  
Ting Xiao
Keyword(s):  
Machine Learning ◽  
Genomic Instability ◽  
Cell Lines ◽  
Prognostic Model ◽  
Expression Profiles ◽  
Support Vector ◽  
Learning Methods ◽  
Machine Learning Methods ◽  
A375 Cell ◽  
Melanoma Patients

Background. Melanoma is a common tumor characterized by a high mortality rate in its late stage. After metastasis, current treatment methods are relatively ineffective. Many studies have shown that long noncoding RNA (lncRNA) may participate in gene mutation and genomic instability in cancer. Methods. We downloaded transcriptome data, mutation data, and clinical follow-up data of melanoma patients from The Cancer Genome Atlas. We divided samples into groups according to the number of somatic cell mutations and then performed a differential analysis to screen out the differentially expressed genes. We then divided samples into genomic unstable and genomic stable groups. We compared lncRNA expression profiles in these groups and constructed a protein-coding genes network coexpressed with selected lncRNA to analyze the pathways enriched by these genes. Two machine learning methods, least absolute shrinkage and selector operation (LASSO) and support vector machine-recursive feature elimination (SVM-RFE), were applied to conduct the lncRNA-related prognostic model. Afterward, we performed survival analysis, risk correlation analysis, independent prognostic analysis, and clinical subgroup model validation. Finally, through wound healing assay and transwell assay, the function of AATBC was verified by A375 cell lines. Results. We screened 61 prognostic-related lncRNAs and constructed an lncRNA-mRNA coexpression network based on these lncRNAs. Seven lncRNAs were selected as common characteristic factors based on the two machine learning methods. The model formula was as follows: risk score = 0.085 ∗ AATBC + 0.190 ∗ AC026689.1−0.117 ∗ AC083799.1 + 0.036 ∗ AC091544.6−0.039 ∗ LINC01287−0.291 ∗ SPRY4.AS1 + 0.056 ∗ ZNF667.AS1. The seven lncRNAs in this formula are key candidates. Cell experiments have verified that knocking down AATBC in A375 cell lines can reduce the proliferation and invasion ability of melanoma cells. Conclusion. The lncRNA we identified provides a new way to study lncRNA’s role in the genomic instability of melanoma. Our findings may provide essential candidate biomarkers for the diagnosis and treatment of melanoma.

Naringin and 5-fluorouracil suppress inflammatory Cytokines in human skin cancer cell line

2021 ◽  
Vol 12 (1) ◽  
pp. 729-733
Author(s):  
Deepa Suruli ◽  
Fathima Bushra Sheriff Mirza ◽  
Gloria Jemmi Christobel R ◽  
Amuthavalli Kottaiswamy ◽  
Shila Samuel ◽  
...  
Keyword(s):  
Skin Cancer ◽  
Cell Line ◽  
Human Skin ◽  
Cell Lines ◽  
Cancer Cell ◽  
A375 Cell ◽  
Ic50 Values ◽  
Anti Inflammatory ◽  
A375 Cells ◽  
Inflammatory Effect

Naringin is a citrus flavonoid recently studied for anti-inflammatory activity in numerous cancer cells. In this study, the anti-inflammatory properties of naringin along with 5-fluorouracil in human skin cancer cell lines A375 was analyzed. A375 cells were treated with naringin, 5-fluorouracil alone, and combination. MTT assay and cell viability assays was demonstrated to detect the inhibitory effects of naringin or 5-fluorouracil on cell proliferation. mRNA expression of TNFα, IL-6, IL-1β, and NFκB were determined using quantitative RT-PCR.  The effect of naringin and 5-fluorouracil combination significantly inhibited the growth and proliferation of the A375 cells in a concentration dependent manner with the IC50 values of naringin (24.75 μM) 5-fluorouracil (2.5 μM). The combination of naringin+5-fluorouracil on A375 cell lines at a concentration of half IC50 values (12µM+1 μM). Naringin and 5-fluorouracil combination also decreased the level of TNFα, IL-6, IL-1β, and NFκB mRNA in the A375 cell line. Naringin and 5-fluorouracil exerted anti-inflammatory effect through the suppression of NF-kB, IL-1β, TNFα, IL-6 in A375 cells. Taken together, our results suggested that treating A375 with naringin and 5-fluorouracil combination may have future applications in treating skin cancers through its anti-inflammatory effect.

A highly selective, sensitive and reversible fluorescence chemosensor for Zn2+ and its cell viability

2016 ◽  
Vol 45 (9) ◽  
pp. 3927-3935 ◽  
Author(s):  
Anoop Kumar Saini ◽  
Mansi Srivastava ◽  
Vinay Sharma ◽  
Veenu Mishra ◽  
Shaikh M. Mobin
Keyword(s):  
Detection Limit ◽  
Cell Viability ◽  
Cell Lines ◽  
Sensing Properties ◽  
A375 Cell ◽  
Mcf 7

A new ligand (H2L) was synthesized and designed for sensing properties. H2L shows fluorescence ‘switch ON’ with Zn2+ ions with a detection limit of 1.47 μM. The H2L and 1 showed marginal toxicity against MCF-7 and A375 cell lines.

Virtual Screening for Type II B Inhibitors of B-RafV600E Kinase

2020 ◽  
Vol 16 (3) ◽  
pp. 222-230
Author(s):  
Kai-Xiong Qiu ◽  
Wen Zhang ◽  
Fang Yu ◽  
Wei Li ◽  
Zhong-Wen Sun ◽  
...  
Keyword(s):  
Virtual Screening ◽  
Cell Lines ◽  
Binding Free Energy ◽  
Type Ii ◽  
Combined Approach ◽  
Pharmacophore Modelling ◽  
A375 Cell ◽  
Important Target ◽  
Zinc Database ◽  
Inhibitory Activities

Background: B-RafV600E kinase was identified as an important target in current cancer treatment, and the type II B inhibitors show good qualities in preclinical studies. Therefore, it is very important to discover novel II B inhibitors of B-RafV600E kinase. Methods: In order to discover novel II B inhibitors of B-RafV600E kinase, virtual screening against ZINC database was performed by using a combination of pharmacophore modelling, molecular docking, 3DQSAR model and binding free energy (ΔGbind) calculation studies. The inhibitory activities against A375 cell lines of the hit compounds were tested by using MTT assay. Results: Five promising hit compounds were obtained after screening, and all the five hit compounds showed good inhibitory rates against A375 cell lines. Conclusion: The combined approach of the virtual screening in our work is effective, which can be used to discover novel inhibitors with a new skeleton. In addition, the five compounds obtained from the screening showed good inhibitory rates against A375 cell lines, which can be considered to develop new II B inhibitors of B-RafV600E kinase.

scholarly journals Triterpenoid Saponin and Lignan Glycosides from the Traditional Medicine Elaeagnus angustifolia Flowers and Their Cytotoxic Activities

Molecules ◽  
2020 ◽  
Vol 25 (3) ◽  
pp. 462 ◽  
Author(s):  
Jianxin Han ◽  
Xiaoyu Chen ◽  
Wei Liu ◽  
Hao Cui ◽  
Tao Yuan
Keyword(s):  
Traditional Medicine ◽  
Cell Lines ◽  
Spectroscopic Data ◽  
Cytotoxic Activities ◽  
Triterpenoid Saponin ◽  
Elaeagnus Angustifolia ◽  
A375 Cell ◽  
Extensive Analysis ◽  
Ic50 Values ◽  
Lignan Glycosides

A new triterpenoid saponin, named terpengustifol A (1), and two new lignan glucosides, phengustifols A and B (2 and 3), were isolated from the flowers of Elaeagnus angustifolia. Their structures were determined by the extensive analysis of the spectroscopic data (including NMR and HRMS) and ECD calculations. Compound 1 possesses an unusual monoterpene (Z)-6-hydroxy-2,6-dimethylocta-2,7-dienoyl unit at C-21. Compounds 2 and 3 are a pair of diastereoisomers, while their aglycones are a pair of enantiomers. Compounds 1 and 2 exhibited moderate cytotoxic activities against A375 cell lines with IC50 values at 12.1 and 15.6 μM, respectively. This is firstly reported the triterpenoid saponin and lignans isolated from the Elaeagnus angustifolia flowers.

Alpha-1-Antitrypsin Antagonizes Cisplatin-Induced Cytotoxicity in Prostate Cancer (PC3) and Melanoma Cancer (A375) Cell Lines

2016 ◽  
Vol 23 (2) ◽  
pp. 335-343 ◽  
Author(s):  
Mila Ljujic ◽  
Sanja Mijatovic ◽  
Mirna Z. Bulatovic ◽  
Marija Mojic ◽  
Danijela Maksimovic-Ivanic ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Lines ◽  
Melanoma Cancer ◽  
A375 Cell ◽  
Alpha 1 Antitrypsin

(EB) Virus: Latency and Expression in Transplanted and Cultured Human Cells

1971 ◽  
Vol 29 ◽  
pp. 368-369
Author(s):  
B. G. Uzman ◽  
M. M. Kasac ◽  
H. Saito ◽  
A. Krishan
Keyword(s):  
Cell Lines ◽  
Primary Cultures ◽  
Virus Particles ◽  
Barr Virus ◽  
Virus Latency ◽  
Virus Surveillance ◽  
Cultured Human Cells ◽  
Epstein Barr ◽  
Serial Transplantation ◽  
Tubular Arrays

In conjunction with the cultivation and transplantation of cells from human tumors by the Programs of Microbiology and Immunogenetics, virus surveillance by electron microscopy has been routinely employed. Of particular interest in this regard have been 3 cell lines cultured from lymph nodes or spleen of 2 patients with Hodgkin's disease and 1 patient with Letterer-Siwe's disease. Each of these cell lines when transplanted in Syrian hamster neonates conditioned with anti-lymphocyte serum grew as serially transplantable tumors; from such transplants of the 3 cell lines cell cultures were retrieved.Herpes type virus particles (Figs. 1, 2, 3) were found in the primary cultures of all three lines, in frozen thawed aliquots of same, and in cultures retrieved from their tumors growing by serial transplantation in hamsters. No virus was detected in sections of 25 of the serially transplanted tumors. However, in 10 such tumors there were repeated instances of tubular arrays in the cisternae of the endoplasmic reticulum (Fig. 4). On serologic examination the herpes virus was shown to be the Epstein-Barr virus.

Comparison of Choriocarcinoma and Normal Placenta

1971 ◽  
Vol 29 ◽  
pp. 324-325
Author(s):  
John C. Garancis ◽  
Roland A. Pattillo ◽  
Robert O. Hussa ◽  
Jon V. Straumfjord
Keyword(s):  
Cell Lines ◽  
Small Cells ◽  
Tissue Cultures ◽  
Glycogen Depletion ◽  
Cell Surfaces ◽  
Intercellular Spaces ◽  
Concomitant Increase ◽  
Gestational Choriocarcinoma ◽  
Cytoplasmic Glycogen ◽  
Normal Placenta

Two different cell lines (Be-Wo and Jar) of human gestational choriocarcinoma have been maintained in continuous tissue culture for a period of four and two years respectively without losing the ability to elaborate human chorionic gonadotropin (HCG). Tissue cultures, as revealed by electron microscopy, consisted of small cells with single nuclei. In some instances cell surfaces were provided with microvilli but more often the intercellular spaces were narrow and bridged by desmosomes. However, syncytium was not formed. Endoplasmic reticulum (ER) was poorly developed in both cell lines, except in some Be-Wo cells it was prominent. Golgi complex, lysosomes and numerous free ribosomes, as well as excessive cytoplasmic glycogen, were present in all cells (Fig. 1). Glycogen depletion and concomitant increase of ER were observed in many cells following a single dose of 10 ugm/ml of adrenalin added to medium (Fig. 2).

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