Cold Pretreatment-Induced Changes in Antioxidant Enzyme Activities and Relative Water Content and Soluble Sugars in Shoots and Roots of Soybean Seedlings

2007 ◽  
Vol 7 (8) ◽  
pp. 1525-1530 ◽  
Author(s):  
Leila Zeinali Yadegari ◽  
Reza Heidari . ◽  
Jirair Carapetian .
Keyword(s):  
Water Content ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Relative Water Content ◽  
Soluble Sugars ◽  
Antioxidant Enzyme Activities ◽  
Cold Pretreatment ◽  
Soybean Seedlings ◽  
Relative Water ◽  
Induced Changes

scholarly journals Antioxidant enzyme activities in Allium species and their cultivars under water stress

2008 ◽  
Vol 53 (No. 12) ◽  
pp. 517-523 ◽  
Author(s):  
J. Csiszár ◽  
E. Lantos ◽  
I. Tari ◽  
E. Madoşă ◽  
B. Wodala ◽  
...  
Keyword(s):  
Water Stress ◽  
Water Content ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Moisture Stress ◽  
Water Withdrawal ◽  
Antioxidant Enzyme Activities ◽  
Glutathione S Transferase ◽  
Soil Moisture Stress ◽  
Relative Water

We compared the enzymatic antioxidative defence mechanisms of some regional subspecies of <I>Allium (A. cepa </I>L., <I>A. ascalonicum</I> auct. hort., A.<I> sativum</I> L.) cultivated mainly in the western regions of Romania, and two modern Hungarian climate resistant F <sub>1</sub> hybrids. The variability in the activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), glutathione reductase (GR) and glutathione S-transferase (GST) and their changes under soil moisture stress were investigated. 1-week-long water stress revealed that among three <I>Allium</I> species, relative water content decreased only in <I>A. ascalonicum</I> leaves (up to 16%). Unlike root enzymes, the activities of the shoot enzymes, especially POD, GR and GST showed a stronger correlation with the water content of the leaves after one week of water withdrawal; regression coefficients (<I>R</I><sup>2</sup>) were 0.359, 0.518 and 0.279, respectively. The ancient populations with elevated (or highly inducible) antioxidant enzyme activities may be interesting for further research and for breeding of new <I>Allium</I> varieties.

scholarly journals Antioxidant Enzymatic Activities and Growth Response of Quinoa (Chenopodium quinoa Willd) to Exogenous Selenium Application

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 719
Author(s):  
Ahlam Khalofah ◽  
Hussein Migdadi ◽  
Ehab El-Harty
Keyword(s):  
Water Content ◽  
Photosynthetic Pigments ◽  
Relative Water Content ◽  
Defense Mechanism ◽  
Higher Plants ◽  
Growth Parameters ◽  
Soluble Sugars ◽  
Low Concentrations ◽  
Relative Water ◽  
High Concentrations

Selenium is a trace element essential to many organisms, including higher plants. At low concentrations, it enhances growth and development; however, it is toxic at high concentrations. The development of crops with proper levels of selenium will be worth for both nutrition and Se-based therapeutics. This study aimed to investigate the morphological, physiological, and biochemical responses of the quinoa plant to 0, 2.5, 5, 10, and 20 mg/L of Na2SeO3·5H2O. Selenium at low concentrations (2.5 and 5 mg/L), quinoa plant showed a significant increase of growth parameters, relative water content, photosynthetic pigments, proline, total soluble sugars, and antioxidant enzymes activities as (superoxide dismutase (SOD), catalase (CAT), peroxidase (POD, ascorbate peroxidase (APX), and glutathione reductase (GR)), and contents of malondialdehyde (MDA) and H2O2 were reduced. However, high concentrations (10 and 20) mg/L caused a decrease in plant growth parameters, relative water content, and photosynthetic pigments. In contrast, excess selenium increased the oxidative stress monitored by hydrogen peroxide and lipid peroxidation levels. The enzymatic antioxidant system responded to the selenium supply significantly increased. Osmolytes compounds, such as total sugars and proline, increased in selenium-treated plants. The increase in these osmolytes compounds may show a defense mechanism for the osmotic readjustment of quinoa plants to mitigate the toxicity caused by selenium. This study shows the morphological and physiological responses that must be considered for success in the sustainable cultivation of quinoa plants in environments containing excess selenium.

scholarly journals Study of Some Mechanisms of Tolerance and Avoidance of Water Deficit in Barley (Hordeum vulgare L.)

2019 ◽  
Vol 67 (6) ◽  
pp. 1503-1512
Author(s):  
Mimouna Zerrouki ◽  
Zineb Regagba ◽  
Ahmed Adda
Keyword(s):  
Hordeum Vulgare ◽  
Drought Tolerance ◽  
Water Supply ◽  
Water Content ◽  
Water Deficit ◽  
Relative Water Content ◽  
Soluble Sugars ◽  
Hordeum Vulgare L ◽  
Relative Water ◽  
Barley Genotypes

Some mechanisms of drought tolerance and avoidance have been studied in eight barley genotypes. These include three local cultivars (Ttichedrett, Beldi, Saida) and five introduced (Malouh, Seg09, Mari29, Awblack, Beecher) which were grown under three different water supply (100% FC, 60% FC, 30% FC). The increase in intensity of the water deficit reduces differently the relative water content among tested genotypes. Thus, in the treatment of 60% FC, this reduction varies between 1% (Beldi) and 6% (Tichedrett, Beecher), while in the lot of 30% FC it reaches the limits of 3% (Beldi) and 18% (Seg09). The results show that plants grown under water deficit (60% CC, 30% FC) accumulate significantly soluble sugars (r = 0.54**) and K+ (r = 0.26**) compared to control plants (100% CC). Thus, under 30% FC, the accumulation of soluble sugars reached values of 195% and 208% observed at Mari29 and Awblack. The accentuation of the water deficit causes a significant reduction of the RWL particularly after 120 mn (r = -0.27**) which is accompanied by an increase of the stomatic resistance (r = 0.53**). To limit dehydration under the water deficit, tested genotypes were distinguished into three groups by adopting avoidance, tolerance or by combining the two strategies.

Copper and Inhibitors Induced Changes on Subcellular Antioxidant Enzyme Activities in the Leaves of Elsholtzia haichowensis

2012 ◽  
Vol 518-523 ◽  
pp. 5539-5544
Author(s):  
Dong Yuan
Keyword(s):  
Antioxidant Enzymes ◽  
Nadph Oxidase ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Defense Mechanism ◽  
Additional Treatment ◽  
Antioxidant Enzyme Activities ◽  
Cell Compartments ◽  
Elsholtzia Haichowensis ◽  
Induced Changes

Under hydroponics, shoots of the copper (Cu) accumulator Elsholtzia haichowensis after 100 μM CuSO4 treatment 6 day were additionally treated with different inhibitors, then the effects of Cu and inhibitors on antioxidant enzyme activities in leave cells of E. haichowensis were investigated. Our results showed that Cu treatment significantly increased the activities of plasma membrane–bound NADPH oxidase, apoplastic peroxidase, apoplastic superoxide dismutase (SOD), symplastic SOD and symplastic ascorbate peroxidase in leaves of E. haichowensis. When additional treatment with N-N-diethyldithiocarbamate as an inhibitor of SOD, NaN3 as an inhibitor of peroxidase, diphenyleneiodonium as an inhibitor of NADPH oxidase and1,2-dihydroxybenzene-3,5-disulphonic acid as an O2•– scavenger, the activities of Cu-induced antioxidant enzymes in leave cells of E. haichowensis were significantly inhibited. The results showed that Cu-induced increase in the activities of various antioxidant enzymes in different cell compartments may represent a defense mechanism against oxidative stress.

scholarly journals Wound-Induced Changes in Antioxidant Enzyme Activities in Skin Mucus and in Gene Expression in the Skin of Gilthead Seabream (Sparus aurata L.)

Fishes ◽  
2021 ◽  
Vol 6 (2) ◽  
pp. 15
Author(s):  
Cristóbal Espinosa-Ruíz ◽  
María Ángeles Esteban
Keyword(s):  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Stress Proteins ◽  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Antioxidant Enzyme Activities ◽  
Skin Mucus ◽  
Expression Levels ◽  
Induced Changes ◽  
Related Proteins

This study investigated the antioxidant enzyme activities in the skin mucus of gilthead seabream (Sparus aurata L.) at 3 and 7 days post-wounding (dpw). The expression levels of the genes that encode stress proteins (grp170, grp94, grp75, sod and hsp70) and skin regeneration-related proteins (tf, igf1, tgfb1, der1, apo1 and erdj3) in the skin also were determined. Mucus and skin samples were obtained from the left and right flanks of non-wounded and wounded fish. In both flanks of the wounded fish, catalase and glutathione reductase activities in the skin mucus increased (p < 0.05) at 3 and 7 dpw (100 ± 31% and 111 ± 25%, respectively), whereas superoxide dismutase activity increased (p < 0.05) only at 7 dpw (135 ± 15%). The expression levels of stress proteins in the skin of the wounded flank of the wounded fish mainly increased at 7 dpw (grp170 increased to 288 ± 85%, grp94 to 502 ± 143%, grp75 to 274 ± 69%, sod to 569 ± 99%, and hsp70 increased to 537 ± 14%) (p < 0.05). However, the expression levels of the tissue regeneration-related genes varied depending on the flank investigated, on the experimental time, and on the gene studied. To the best of our knowledge, this is the first work to determine the effect of a wound in different skin parts of the same fish.

scholarly journals Foliar application of melatonin alleviates the effects of drought stress in rice (Oryza sativa L.) seedlings

2021 ◽  
Vol 49 (3) ◽  
pp. 12417
Author(s):  
Parichart SILALERT ◽  
Wattana PATTANAGUL
Keyword(s):  
Drought Stress ◽  
Plant Growth ◽  
Enzyme Activities ◽  
Electrolyte Leakage ◽  
Relative Water Content ◽  
Foliar Application ◽  
Antioxidant Enzyme Activities ◽  
Rice Seedlings ◽  
Relative Water ◽  
Effects Of Drought

Melatonin (N-acetyl-5-methoxytryptamine) plays an essential role in abiotic stress in plants, but its mechanism in drought tolerance is unclear. To better understand the protective roles of melatonin against drought stress, we investigated the effect of foliar application with exogenous melatonin on plant growth, physiological responses, and antioxidant enzyme activities in rice seedlings under drought stress. Rice seedlings were grown for 21 days, and foliar sprayed with 0, 50, 100, 200, and 300 µM melatonin. The control plant was watered daily, while the others were subjected to drought stress by withholding water for seven days. The results showed that drought stress significantly reduced plant growth, relative water content, and chlorophyll content. Electrolyte leakage, malondialdehyde (MDA) content and hydrogen peroxide (H2O2) were also negatively affected by drought stress. Application of melatonin alleviated the effects of drought stress by increasing plant growth, improving relative water content and chlorophyll content, and decreasing electrolyte leakage, MDA, and H2O2. Foliar application with melatonin also increased antioxidant enzyme activities, including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and guaiacol peroxidase (GPX). In addition, melatonin also enhanced proline and total soluble sugar accumulation during drought stress. It is, therefore, suggested that foliar application with 100 µM melatonin was the most effective for reducing the adverse effects of drought stress in rice plants.

Sign in / Sign up

Export Citation Format

Share Document