Phenolic acids and ruminal parameters of different varieties of sugarcane in natura or ensiled

This study aimed to determine and measure the phenolic acids in four varieties of sugarcane (RB 855536, RB 765418, SP 80-1842 and SP 80-1816) as fresh and ensiled, and assess the influence of these acids on in situ degradability of dry matter and neutral detergent fiber. The experimental design was completely randomized in a factorial design. For the fraction of dry matter (DM), varieties RB 855536 and SP 80-1842 had average 17.80% higher compared to others. The SP80-1842 and RB 855536 varieties did not differ (p > 0.05), with an average of DM effective degradability of 33.70%. For silage, the highest levels of p-coumaric acid were found in the variety RB 855536, which was 24.64% higher than the varieties RB 765418 and SP 80-1842 (average 12.75%) and 52.60% of the variety SP 80-1816 (average 8.02%), since for the in natura form, varieties did not differ between each other (p > 0.05), and had an average of 8.86%. Silage variety SP 80-1816 has ferulic acid content 40.82% higher than the other varieties, with an average of 4.63%. The examination of the concentrations of phenolic acids p-coumaric and ferulic are not sufficient to justify the effect of microbial action on the varieties studied in different forms of processing.

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Silages of pearl millet submitted to nitrogen fertilization

Ciência Rural ◽

10.1590/s0103-84782014000500025 ◽

2014 ◽

Vol 44 (5) ◽

pp. 918-924 ◽

Cited By ~ 1

Author(s):

Ricardo Martins Araujo Pinho ◽

Edson Mauro Santos ◽

Fleming Sena Campos ◽

João Paulo de Farias Ramos ◽

Carlos Henrique Oliveira Macedo ◽

...

Keyword(s):

Lactic Acid ◽

Chemical Composition ◽

Experimental Design ◽

Pearl Millet ◽

Nitrogen Fertilization ◽

Acid Content ◽

Dry Matter ◽

Neutral Detergent Fiber ◽

Lactic Acid Content ◽

Split Plot

This study aimed to evaluate the fermentation characteristics, losses and the chemical composition of two pearl millet genotypes silages submitted to nitrogen fertilization. The experimental design was a completely randomized blocks in a split plot scheme 2x5 (two nitrogen genotypes doses x five doses of nitrogen), with four replicates. Nitrogen doses were 0, 20, 40, 60, 80kg ha-1 and the pearl millet genotypes were the variety ADR300 and the hybrid ADR7010. The hybrid ADR 7010 showed average lactic acid content higher than the variety ADR 300, at all doses of N, recording values ranging from 4.09 to 10.46dag kg-1. There was an interaction between nitrogren doses and genotypes for the neutral detergent fiber, which ranged from 51.81 to 63.63dag kg-1 of dry matter. Dry matter recovery decreased linearly with increasing nitrogen doses only for hybrid ADR7010, the same did not happen for the ADR300. The nitrogen fertilization does not favor the fermentation characteristics and increases DM losses of the hybrid ADR7010.

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In situ degradability of elephant grass ensiled with increasing levels of pineapple agro-industrial by-product

Semina Ciências Agrárias ◽

10.5433/1679-0359.2016v37n4supl1p2807 ◽

2016 ◽

Vol 37 (4Supl1) ◽

pp. 2807 ◽

Cited By ~ 2

Author(s):

Ana Cristina Holanda Ferreira ◽

Norberto Mario Rodriguez ◽

José Neuman Miranda Neiva ◽

Patrícia Guimarães Pimentel ◽

Silas Primola Gomes ◽

...

Keyword(s):

Experimental Design ◽

Crude Protein ◽

Dry Matter ◽

Corn Silage ◽

Neutral Detergent Fiber ◽

Elephant Grass ◽

Acid Detergent Fiber ◽

Potential Alternative

The objective of this study was to evaluate the in situ degradability of dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), and hemicellulose of elephant grass (Pennisetumpurpureum, Schum.) with increasing levels (0; 35.0; 70.0, 105.0, and 140.0 g kg-1) of dried pineapple (Ananascomosus, L.) by-product (DPBP). The experimental silos consisted of plastic drums with 210 L capacity. After weighing and homogenizing the elephant grass with the DABP, the material was inserted in the silos (126 kg silage, at a density of 600 kg m-3) and compressed. After 45 days of ensilage, silos were opened and samples of the silages were collected for the trial. The study of degradability in situ was conducted using a rumen-fistulated male cattle. The animal was fed with corn silage supplemented with 1 kg concentrate daily. Five levels of addition of the by-product were tested, with three replicates and five times of incubation with an animal. The experimental design was completely randomized with split plots, in which the proportions of DPBP were the treatments (plots), the different silos were the replicates, and the incubation times were the subplots. The levels of inclusion of DPBP provided an increase (P < 0.05) in disappearance of DM and CP and in the effective degradability of DM, NDF, ADF, and hemicellulose. The dried pineapple by-product is a potential alternative to be used as additive in the ensilage of elephant grass, as it provides an increase in the rumen degradability parameters.

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In situ ruminal degradation of phenolic acid, cellulose and hemicellulose in crop brans and husks differing in ferulic and p-coumaric acid patterns

The Journal of Agricultural Science ◽

10.1017/s0021859615000489 ◽

2015 ◽

Vol 153 (7) ◽

pp. 1312-1320 ◽

Cited By ~ 3

Author(s):

B. B. CAO ◽

R. WANG ◽

H. J. YANG ◽

L. S. JIANG

Keyword(s):

Ferulic Acid ◽

Phenolic Acids ◽

Phenolic Acid ◽

Coumaric Acid ◽

Forage Crops ◽

Maize Bran ◽

Cereal Brans ◽

Ruminal Degradation ◽

Ruminant Animals

SUMMARYLignification-associated phenolic acids are widely distributed in graminaceous plant cell walls. Nylon bags containing maize bran, wheat bran, millet husk and rice husk were incubated in the rumens of five Charolais (♂) × Nanyang (♀) crossbred steers for 6, 12, 24, 36, 48 and 72 h. The in situ ruminal disappearance of ester-linked phenolic acids linearly increased in the brans with increasing incubation time, and the disappearance was greater for ester-linked ferulic acid (FAest) than for ester-linked p-coumaric acid (PCAest). The disappearances of FAest and PCAest were positively correlated with disappearances of neutral detergent fibre (NDF), cellulose and hemicellulose. The effective degradabilities of NDF, cellulose and hemicellulose in the brans were markedly greater than the effective degradabilities of these components in the husks, and were negatively correlated with the contents of Lignin (sa), ether-linked ferulic acid, PCAest and ether-linked p-coumaric acid in both the cereal brans and husks. These findings suggested that breeding forage crops with modified phenolic acid contents could represent an alternative strategy to promote further increases in fibre digestibility of cereal residue feeds for ruminant animals.

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Potential degradation and colonization time of ruminal microorganisms on the particles of different oilseeds-crushed, and its chemical composition

Bioscience Journal ◽

10.14393/bj-v37n0a2021-48122 ◽

2021 ◽

Vol 37 ◽

pp. e37001

Author(s):

Rafael Henrique de Tonissi Buschinelli de Goes ◽

Kennyson Alves de Souza ◽

Milene Puntel Osmari ◽

Thiago José de Lira Cardoso ◽

Raquel Tenório de Oliveira ◽

...

Keyword(s):

Organic Matter ◽

Chemical Composition ◽

Crude Protein ◽

Dry Matter ◽

Neutral Detergent Fiber ◽

The Other ◽

Biodiesel Production ◽

Ether Extract ◽

Colonization Time

This study was carried out to evaluate by-product of the biodiesel industry as canola, safflower, forage turnip, and soybean crushed on the chemical composition, in situ degradability, and colonization time. Canola (Brassica napus L. var. oleifera), safflower (Carthamus tinctorius L.), forage turnip (Raphanus stivus L. var. oleiferus Metzg), and soybean (Glycine max) grains went through the oil extraction process by means of a cold pressing, resulting in the oilseeds-crushed. The treatments identification included: CAN – Canola crushed; SAF – Safflower crushed; TUR – Forage turnip crushed; and SOY – Soybean crushed. The oilseed-crushed treatments were quantified about mineral (calcium, magnesium, copper, iron, manganese, zinc, phosphorus and potassium), chemical composition (dry matter, ash, organic matter, crude protein, ether extract, neutral detergent fiber, acid detergent fiber, total carbohydrates, non-fibrous carbohydrates, and total digestible nutrient contents), In situ degradability, and colonization time. Magnesium, phosphorus, and zinc showed the greater values for TUR treatment 3.46, 27.4, 39.8, respectively, when compared to the other oilseed-crushed treatments. The TUR treatment had the lowest organic matter, whereas had the greater (p≤0.05; TUR and SOY treatments) for the non-fibrous carbohydrates. Ether extract was not affected (p>0.05) with the different oilseed-crushed treatments. Neutral detergent fiber was affected (p≤0.05) for CAN and SAF treatments with the greater values, 344 and 500 g/kg of dry matter, respectively. Soluble and potentially degradable fractions for SAF treatment showed similar results. Whereas the constant rate of degradation, presented the lowest value when compared to the other treatments. Effective degradability of crude protein was greater for CAN 63.2% than SOY 65.9% treatment, which had the lowest value. Potential degradability of crude protein did not differ between treatments. Colonization time for dry matter and crude protein were similar between TUR and SOY treatments. In conclusion, oilseeds-crushed from the biodiesel production can be targeted/used, as feed with great protein and energetic potential in the ruminant’s production, considering the need of correct formulation and ingredients knowledge.

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Evaluation of the Nutrient Composition, In Vitro Fermentation Characteristics, and In Situ Degradability of Amaranthus caudatus, Amaranthus cruentus, and Amaranthus hypochondriacus in Cattle

Animals ◽

10.3390/ani11010018 ◽

2020 ◽

Vol 11 (1) ◽

pp. 18

Author(s):

Kim Margarette C. Nogoy ◽

Jia Yu ◽

Young Gyu Song ◽

Shida Li ◽

Jong-Wook Chung ◽

...

Keyword(s):

Crude Protein ◽

Dry Matter ◽

Gas Production ◽

Nutrient Composition ◽

Neutral Detergent Fiber ◽

Amaranthus Cruentus ◽

Amaranthus Caudatus ◽

Amaranthus Hypochondriacus

The amaranth plants showed high potential feed value as forage for ruminants. An in-depth study of this plant, particularly in cattle, will help extend its utilization as an alternative protein and fiber feed source in cattle feeding. In this study, the nutrient compositions of three different species of amaranth, Amaranthus caudatus L., Amaranthus cruentus L., and Amaranthus hypochondriacus L.—two varieties for each species, A.ca 74, A.ca 91, A.cu 62, A.cu 66, A. hy 30, and A. hy 48—were evaluated. The in vitro technique was used to evaluate the fermentation characteristics such as total gas production, total volatile fatty acids (VFA) concentration, pH, and ammonia concentration of the rumen fluid. Moreover, the effective degradabilities of dry matter (EDDM) and crude protein (EDCP) of the amaranth forages were determined through in situ bag technique. The amaranth forages: A. caudatus, A. cruentus, and A. hypochondriacus showed better nutritive value than the locally produced forages in Chungcheong province of Korea. The CP of the amaranth ranged from 11.95% to 14.19%, and the neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents ranged from 45.53% to 70.88% and 34.17% to 49.83%, respectively. Among the amaranth varieties, A. hypochondriacus 48 showed the most excellent ruminant feed nutrient quality (CP, 14.19%; NDF, 45.53%; and ADF, 34.17%). The effective degradabilities of dry matter (EDDM; 33–56%) and crude protein EDCP (27–59%) of the amaranth were lower compared to other studies, which could be due to the maturity stage at which the forages were harvested. Nonetheless, A. hypochondriacus 48 showed the highest EDDM (56.73%) and EDCP (59.09%). The different amaranth species did not differ greatly in terms of total VFA concentration or molar proportions, total gas production, or ammonia-N concentration. The high nutrient composition, and highly effective degradability of dry matter and crude protein, coupled with the favorable fermentation characteristics, suggest that the amaranth forages showed good to excellent feed quality for cattle.

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In situ degradability of dry matter and fibrous fraction of sorghum silage

Acta Scientiarum Animal Sciences ◽

10.4025/actascianimsci.v38i2.29576 ◽

2016 ◽

Vol 38 (2) ◽

pp. 171 ◽

Cited By ~ 1

Author(s):

Renê Ferreira Costa ◽

Daniel Ananias de Assis Pires ◽

Marielly Maria Almeida Moura ◽

José Avelino Santos Rodrigues ◽

Vicente Ribeiro Rocha Júnior ◽

...

Keyword(s):

Experimental Design ◽

Dry Matter ◽

Degradation Rate ◽

Degradation Kinetics ◽

Grain Sorghum ◽

Dual Purpose ◽

Ruminal Degradability ◽

Sorghum Silage ◽

Kinetics Of

This study aimed to evaluate in situ degradability and degradation kinetics of DM, NDF and ADF of silage, with or without tannin in the grains. Two isogenic lines of grain sorghum (CMS-XS 114 with tannin and CMS-XS 165 without tannin) and two sorghum hybrids (BR-700 dual purpose with tannin and BR-601 forage without tannin) were ensiled; dried and ground silage samples were placed in nylon bags and introduced through the fistulas. After incubation for 6, 12, 24, 48, 72 and 96 hours, bags were taken for subsequent analysis of fibrous fractions. The experimental design was completely randomized with 4 replicates and 4 treatments and means compared by Tukey’s test at 5% probability. As for the DM degradation rate, silage of CMSXS165without tannin was superior. Silages of genotypes BR700 and CMSXS 114 with tannin showed the highest values of indigestible ADF (59.54 and 43.09%). Regarding the NDF, the potential degradation of silage of CMSXS165 line without tannin was superior. Tannin can reduce ruminal degradability of the dry matter and fibrous fractions.

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Ruminal degradation characteristics of corn and corn–sunflower intercropped silages prepared at two stages of maturity

Canadian Journal of Animal Science ◽

10.4141/cjas92-100 ◽

1992 ◽

Vol 72 (4) ◽

pp. 881-889 ◽

Cited By ~ 3

Author(s):

Z. Mir ◽

P. S. Mir ◽

S. Bittman ◽

L. J. Fisher

Keyword(s):

Dry Matter ◽

Degradation Rate ◽

Soluble Fraction ◽

Corn Silage ◽

Neutral Detergent Fiber ◽

Acid Detergent Fiber ◽

Ruminal Degradation ◽

Whole Plants ◽

Two Stages

The degradation characteristics of dry matter (DM), protein, neutral detergent fiber (NDF) and acid detergent fiber (ADF) of corn–sunflower intercrop silage (CSS) and monoculture corn silage (CS) prepared from whole plants, harvested at two stages of maturity, were compared using eight mature rumen-cannulated steers. The degradation characteristics were determined by incubating the silages in nylon bags for up to 72 h in the rumen of animals fed the respective silages. The degradation characteristics determined for the silages were the soluble fraction, the insoluble but degradable fractions, the rate of degradation of the degradable fractions of silage DM and protein, and the in situ disappearance of NDF and ADF after 0.5 and 72 h of incubation. The rate of particulate passage from the rumen was determined using chromium-mordanted NDF of the four silages. Values were used to estimate effectively degraded DM and protein. The rates of DM and protein degradation were highest for late-cut CSS (6.3 and 6.0% h−1, respectively) and the least for late-cut CS (2.5 and 0.8% h−1, respectively). Averaged across stages of maturity, more (P < 0.05) DM and protein were effectively degraded with CSS (57.4 and 70.1%, respectively) than with CS (48.8 and 48.7%). Degradation of NDF in early-cut CSS was lower (P < 0.05) than in CS after 72 h of incubation. ADF disappearance from all of the silages after 72 h of incubation was similar. Ruminal degradation of DM and protein in CSS was greater than in CS, which may affect efficiency of utilization of CSS. Key words: Degradation rate, effective degradability, corn silage, intercropped corn–sunflower, steers

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Degradación microbiana in situ de tejidos foliares de gramíneas y leguminosas y su relación con indicadores de calidad nutricional

Ciencia y Tecnología Agropecuaria ◽

10.21930/rcta.vol6_num1_art:41 ◽

2005 ◽

Vol 6 (1) ◽

pp. 100

Author(s):

Diego Chamorro ◽

Juan Evangelista Carulla ◽

Pablo Cuesta

Keyword(s):

Nutritional Quality ◽

Dry Matter ◽

Neutral Detergent Fiber ◽

Dry Matter Degradation ◽

Leaf Tissues ◽

Dry Seasons ◽

Stylosanthes Scabra ◽

Negative Relationships

Para cuantificar la degradación in situ de tejidos vegetales y su relación con la composición química de especies forrajeras se seleccionaron láminas foliares de las gramíneas Bouteloua repens y Bothriochloa pertusa y foliolos de las leguminosas Stylosanthes scabra, Desmodium barbatum y Tephrosia cinerea. En el rumen se incubaron muestras de 10 mm de largo durante 0, 12, 24, 48 y 72 horas; para la lectura del área residual se digitalizaron ocho tejidos. A las 24 y 48 h de incubación B. pertusa presentó relaciones positivas entre la degradación de la epidermis adaxial (EA) y la FDN (R2= 90,2), entre el mesófilo y la DIVMS (R2=80,1), y entre los tejidos lentamente degradables (TLD) y la FDA (R2= 83,9); y relaciones negativas entre EA y DIVMS (R2= –73,1), los TLD con la DEF (R2= –74,3), la EA con la PC (R2= –87,6), y el esclerenquima con la DIVMS y la PC (R2= –84,3 y R2= –90,8). Después de 72 horas de incubación las mayores áreas residuales en gramíneas fueron la estructura kranz entre 34,2% y 36,5%, el mesófilo entre 20,9% y 21,4%, el xilema de 11,5% a 17,1% y la EA entre 11,8% y 13,9%; no obstante el esclerénquima se relacionó estrechamente con indicadores de calidad nutricional. Las gramíneas obtuvieron mayores porcentajes de tejidos no degradables que las leguminosas; por lo tanto, la DIVMS y la DEF fueron menores. Entre las leguminosas, S. scabra, presentó la mayor digestibilidad de los tejidos rápidamente degradables, tanto en sequía como en lluvia; en la época seca se incrementó en 134,6% la degradación de estos tejidos. Adicionalmente, en esta especie el área residual de los taninos no presentó relación directa con la degradación del mesófilo. En leguminosas la tasa y extensión de la degradación de las epidermis se incrementaron marcadamente a mayor tiempo de incubación, presentándose diferencias entre especies, situación que no exhibieron las gramíneas. En D. barbatum la degradación de la EA se asoció con la DIVMS y la DEF, explicando en 77,4% y 72,95% estos porcentajes; de igual manera se reportaron relaciones negativas entre la degradabilidad de la epidermis y los porcentajes de FDN, FDA, lignina y taninos (R2= –0,76; R2= –0,79; R2= –0,53 y R2= –0,76, respectivamente). Microbial in situ degradation of grasses and legumes leaf tissues and its realtionship with nutritional quality and precipitationA study was conducted to measure the in situ de- gradation of plant tissues and its relationship with forage chemical composition in leaves of the grasses Bouteloua repens and Bothriochloa pertusa and of the legumes Stylosanthes scabra, Desmodium barbatum and Tephrosia cinerea. Forage samples of 10 mm in length were incubated in the rumen for 0, 12, 24, 48 and 72 h and tissue residual area was determined by reading the residual area in eight digitalized leaf samples per forage. In B. pertusa, after 24 and 48 h of incubation there were positive relations between the degradation of adaxial epidermis (AE) and the neutral detergent fiber (NDF, R2 = 90.2), between mesophyll and in vitro dry matter degradability (IVDMD, R2 = 80,1), and between slowly degradable tissues (SDT) and the acid detergent fiber (ADF, R2 = 83.9); and negative relationships between AE and IVDMD (R2 = -73,1), between SDT and effective dry matter degradation (EDMD, R2 = -74,3), between AE and crude protein (CP, R2 = -87,6), and between sclerenchyma and IVDMD with CP (R2 = -84,3 and R2 = -90,8, respectively). After 72 h of incubation, the greater residual areas in grasses were the Kranz structure (34.2% to 36.5%), mesophyll (20.9% to 21.4%), xylem (11.5% to 17.1%) and AE (11.8% to 13.9%). The area of sclerenchyma was related closely to indicators of nutritional quality. Overall, the grasses had greater content of non-degradable tissues than the legumes; therefore, their IVDMD and EDMD were lower. Among the legumes, S. scabra showed the greater digestibility of rapidly degradable tissues, both during the rainy and dry seasons. At the dry season, the degradation of these tissues increased by 134.6%. Additionally, in this legume, the content of tannins did not have a direct relationship with the degradation of mesophyll. The rate and extension of the degradation of epidermis in legumes, were noticeably increased with increasing length of incubation, existing differences between species, a situation which was not observed in the grasses. In D. barbatum, the degradation of the AE was positively associated with both the IVDMD and EDMD (R2 = 77,4% and 72,95%, respectively). On the other hand, the degradability of epidermis and the percentage of NDF had negative relations with ADF, lignin and tannins (R2 = -0,76; R2 = -0,79; R2 = -0,53 and R2 = -0,76, respectively).

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Nutritive value of sugarcane silages with different bacterial additives and fermentation periods

Revista Brasileira de Saúde e Produção Animal ◽

10.1590/s1519-9940210212020 ◽

2020 ◽

Vol 21 ◽

Author(s):

Ivone Rodrigues da Silva ◽

Francirose Shigaki ◽

Rosane Cláudia Rodrigues ◽

Ana Paula Ribeiro Jesus ◽

Clésio dos Santos Costa ◽

...

Keyword(s):

Crude Protein ◽

Dry Matter ◽

Nutritional Value ◽

Nutritive Value ◽

Soluble Fraction ◽

B Value ◽

Neutral Detergent Fiber ◽

Acid Detergent Fiber ◽

Fermentation Period

ABSTRACT The objective of this study was to evaluate the nutritive value of sugarcane silage with or without inoculation with P. acidipropionici or L. buchneri, over three fermentation periods. The experimental design was completely randomized in a 3 x 3 inoculant by fermentation period factorial arrangement (without inoculant, inoculant 1, inoculant 2; x three fermentation periods, 10, 60, 90 days). Values of pH, dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), hemicellulose (HEM) and lignin were determined and in situ DM degradability profiles were modelled for parameters a, b and c, potential degradation (A) and effective degradability (ED). The 90 day fermentation yielded a lower pH for both inoculants. There was an interaction between inoculant and fermentation period (P < 0.05) for DM content, with a reduction in silage DM without the additive at 90 days. The CP, HEM, ADF and lignin contents of sugarcane were not influenced by the treatments. The addition of P. acidipropionici provided the lowest NDF content at 10 days and presented a higher fraction a, potential degradation and ED. At 60 days, there was no variation in soluble fraction, the control silage showed a higher fraction b, higher potential degradation and ED. At 90 days of fermentation, L. buchneri silages presented a higher fraction a, degradation rate and DE and a higher b value was obtained in the silage without inoculant. Inoculants are effective in maintaining the silage DM content and nutritional value during prolonged fermentation periods.

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The effects of plant density and irrigation on phenolic content in cauliflower

Horticultural Science ◽

10.17221/60/2016-hortsci ◽

2017 ◽

Vol 44 (No. 4) ◽

pp. 178-185 ◽

Cited By ~ 2

Author(s):

Alina Kałużewicz ◽

Jolanta Lisiecka ◽

Monika Gąsecka ◽

Włodzimierz Krzesiński ◽

Tomasz Spiżewski ◽

...

Keyword(s):

Phenolic Compounds ◽

Ferulic Acid ◽

Gallic Acid ◽

Caffeic Acid ◽

Phenolic Acids ◽

Plant Density ◽

Phenolic Content ◽

Sinapic Acid ◽

Dry Weight ◽

Coumaric Acid

This study was conducted to study the influence of plant density and irrigation on the content of phenolic compounds, i.e., phenolic acids and flavonols in cv. ‘Sevilla’ cauliflower curds. Levels of phenolic acids and flavonols were in the range of 3.0–6.2 mg and 25.4–87.8 mg/100 g of dry weight, respectively, depending on plant density and irrigation. Of the phenolic acids, caffeic acid was detected in the highest amount, followed by p-coumaric acid, sinapic acid, gallic acid, and ferulic acid. Of the two flavonols detected, the levels of quercetin were higher than those of kaempferol. The content of the detected phenolic acids (with the exception of ferulic acid) and both flavonols increased with increasing plant density. Furthermore, the concentration of phenolic compounds (with the exception of ferulic acid) was significantly higher under irrigation.

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