scholarly journals Microbiological water quality monitoring of four ponds from Lagoa do Sino Farm located in São Paulo State, Brazil

2021 ◽  
Vol 43 ◽  
pp. e52901
Author(s):  
Patrícia Alexandre Evangelista ◽  
Mayara Andrade Martins de Souza ◽  
Iuri Emanuel de Paula Ferreira ◽  
Natan de Jesus Pimentel-Filho

This study aimed to evaluate the microbiological quality of the water of four ponds used for irrigation on the Lagoa do Sino Farm, as well as to perform the genotypic characterization of virulence factors in Escherichia coli isolates. Sampling was conducted for 11 months, between 2015 and 2016. Samples were analyzed for the presence of thermotolerant coliforms, E. coli and heterotrophs. DNA was extracted from E. coli isolates, followed by genotypic characterization by polymerase chain reaction. Agricultural activities and pesticides used in the sampling period were documented in order to assess possible relationships between agricultural activities and microbiological water quality. The absence of suitable riparian vegetation around all the ponds was observed, benefiting the entry of organic matter and contaminants in the water body. A high index of thermotolerant coliforms in some months indicated the possibility of the transmission of pathogenic microorganisms in these ponds. The values found in some months were above the regulatory limits for water potability and water intended for irrigation. The agrochemicals used in the period seem to influence the results obtained. All 17 E. coli isolates showed at least one of the virulence genes estA, stx1, stx2, and aatA, indicating enterotoxigenic, enterohaemorrhagic or enteroaggregative nature. The presence of E. coli in the waters may be associated with the presence of animals. The water samples analyzed are not suitable for irrigation of vegetables that are consumed raw and/or low lying fruits ingested without skin removal. It is essential to broaden the control of the use of chemicals, as well as the preservation of riparian vegetation to improve the quality of water used in the farm's agricultural activities.

1995 ◽  
Vol 31 (5-6) ◽  
pp. 19-26 ◽  
Author(s):  
G. J. Medema ◽  
I. A. van Asperen ◽  
J. M. Klokman-Houweling ◽  
A. Nooitgedagt ◽  
M. J. W. van de Laar ◽  
...  

This pilot study was carried out to determine the relationship between microbiological water quality parameters and the occurrence of health complaints among triathletes. Data were collected at an Olympic distance triathlon (n=314) and a run-bike-run (n=81; controls for exposure to fresh water). At the time of the triathlon, the concentrations of Escherichia coli , thermotolerant coliforms, faecal streptococci, entero- and reoviruses, F-specific RNA phages, Salmonella, Campylobacter, Aeromonas, Plesiomonas shigelloides, Pseudomonas aeruginosa and Staphylococcus aureus were examined over the swimming course. Information on the occurrence of health complaints during the competition and in the week thereafter was collected through a written questionnaire. The results show that triathletes and run-bike-runners are comparable with respect to factors other than water exposure (age, sex, training history, physical stress, lower intestinal health complaints during the competition) that may influence the occurrence of health complaints in the week after the competition. Triathletes and run-bike-runners reported gastro-intestinal (7.7% vs 2.5%), respiratory (5.5% vs 3.7%), skin/mucosal (2.6% vs 1.2%), general (3.5% vs 1.2%) and total symptoms (14.8% vs 7.4%) in the week after the event. The health risks for triathletes for all symptom groups are not significantly higher than for run-bike-runners. The geometric mean concentration of faecal indicator bacteria is relatively low: E. coli 170/100 ml; faecal streptococci 13/100 ml, enteroviruses were present at concentrations of 0.1/l. The group of triathletes was homogeneusly and relatively intensely exposed to water; they all swam in the same body of water at the same time and 75% reported to have swallowed freshwater. It was concluded that this study design is suitable to study the relationship between health complaints and microbiological water quality. In the summers of 1993 and 1994, a study will be carried out concerning several run-bike-runs and triathlons in freshwaters of different quality.


2006 ◽  
Vol 69 (8) ◽  
pp. 2029-2035 ◽  
Author(s):  
S. F. TYRREL ◽  
J. W. KNOX ◽  
E. K. WEATHERHEAD

The growth in United Kingdom salad production is dependent on irrigation to maintain product quality. There are concerns that irrigation with poor-quality water could pose a disease risk. This article examines the key issues in the emerging debate on the microbiological quality of water used for salad irrigation in the United Kingdom. The links between irrigation water quality and foodborne disease, and the current international guidance on irrigation water quality, are firstly reviewed. The findings indicate that a number of recent food-poisoning outbreaks have been linked to the consumption of fresh fruits and vegetables and that unhygienic product handling is implicated as the principal source of contamination. There is also credible evidence that salads contaminated in the field, including by irrigation water, can pose a small disease risk at the point of sale. Although irrigation water–quality standards exist in various forms internationally, there is no nationally agreed on standard used in the United Kingdom. This paper then describes the results of a survey conducted in 2003 of United Kingdom irrigation practices that might influence the microbiological quality of salads. The survey showed that surface water is the principal irrigation water source, that overhead irrigation predominates, that the gap between the last irrigation and harvest may be <24 h in many cases, and that current water-quality monitoring practices are generally very limited in scope. This paper concludes with a discussion of the issues emerging from the review and survey, including the need for improved water-quality monitoring, and the problems associated with establishing water-quality standards that could be either too strict or too lax.


2000 ◽  
Vol 124 (3) ◽  
pp. 417-425 ◽  
Author(s):  
M. RUTTER ◽  
G. L. NICHOLS ◽  
A. SWAN ◽  
J. DE LOUVOIS

Results from statutory testing of private water supplies in nine Public Health Laboratories in England were compiled, and the effects of supply class, source, treatment and location on water quality were examined. A total of 6551 samples from 2911 supplies was examined, over a 2-year period, of which 1342 (21%) samples, and 949 (33%) supplies on at least one occasion, failed current regulations for Escherichia coli. Total coliforms, including E. coli, were detected in 1751 (27%) samples from 1215 (42%) supplies. The percentage of samples positive for E. coli was highest in summer and autumn, and lowest in winter. Samples taken from larger supplies and from boreholes were less frequently contaminated than those from other sources. Chlorination, filtration or UV light treatment improved the bacteriological quality of supplies, but still resulted in a low level of compliance with the regulations. The public health implications of the study are discussed.


2021 ◽  
Vol 43 ◽  
pp. e66
Author(s):  
Camila Pereira Montovani ◽  
Cassiana Maria Reganhan Coneglian ◽  
Elaine Cristina Catapani Poletti

The present study aimed to evaluate the characteristics that most influenced the water quality variability of the Atibaia river in the city of Paulínia/SP, the coordinates of the water collection point are given by 22º44'23”(S) and 47º07 ' 40 ”(W), in the dry and rainy seasons, from 2006 to 2016. The data used in this study come from the monitoring of parameters carried out in the spring by a research team, accompanied by periodic collections of surface water samples and analyzes laboratory tests. The parameters addressed included: precipitation, temperature, Hydrogenionic potential (pH), turbidity, thermotolerant coliforms (Escherichia coli (E. coli)), dissolved oxygen (DO), biochemical oxygen demand (BOD), chemical oxygen demand (COD), total nitrogen (Ntotal), total phosphorus (Ptotal), total dissolved solids (TDS), electrical conductivity (EC) and chlorides (Cl-). Water quality was assessed using linear correlation analysis, using the Pearson Correlation coefficient (r), and Multivariate Analysis, using Principal Component Analysis (PCA). The presence of EC, Cl- and COD in both periods indicates quality characteristics related to the mineralization of organic compounds present in the water and the eutrophication process.


2010 ◽  
Vol 61 (10) ◽  
pp. 2521-2530 ◽  
Author(s):  
M. Bougeard ◽  
J. C. Le Saux ◽  
M. Jouan ◽  
G. Durand ◽  
M. Pommepuy

The microbiological quality of waters in estuaries determines their acceptability for recreational uses. Microbiological contamination often results from urban wastewater discharges or non-point source pollution (manure spreading), and can cause bathing zones to be closed. European regulations (EC/7/2006) have proposed standards (500 E. coli/100 ml) for the acceptability areas for bathing. In this study, two models were associated to simulate contamination: SWAT on a catchment and MARS 2D in the downstream estuary. After river flow calibration and validation, two scenarios were simulated in SWAT, and E. coli fluxes obtained at the main outlet of the catchment were then introduced into MARS 2D to follow E. coli concentrations in the estuary. An annual evaluation of compliance to bathing area water quality standards was then calculated, linked with daily rainfall classes. Water quality in the estuary was below the standard on 13 days, including 5 days with rainfall superior to 10 mm, due to faecal contamination from soil leaching by rain, and 5 days with rainfall ranging from 0.1 to 5 mm/day, due to the high frequency of this level of rainfall. To conclude, this study allowed us to demonstrate the efficiency of models to gain a better understanding on water quality degradation factors.


2005 ◽  
Vol 51 (12) ◽  
pp. 33-41 ◽  
Author(s):  
F. Brissaud ◽  
T. Andrianarison ◽  
J.L. Brouillet ◽  
B. Picot

The WSP system serving Mèze and Poussan (French Mediterranean coast) was constructed in 1980 and enlarged and upgraded from 1994 to 1998. Water quality along the waste stabilisation pond to (WSP) system has been monitored over the years, thus allowing us to assess the influence of enlargement and upgrading works. A significant enhancement of the average microbiological quality of the effluent was observed, with respective E. coli and streptococci average abatements of 4.1 and 3.4 log. units. Former seasonal variations of microbiological removal have vanished. The contribution of the different ponds to the disinfection performance of the WSP system was analysed. A microbiological quality model was proposed to evaluate the die-off kinetics related to the different ponds and as a tool for the design and management of WSP systems. Though the relationships between die-off coefficients and environmental factors appeared somewhat frail, this modelling is considered a promising approach for the prediction of WSP microbiological performance.


2013 ◽  
Vol 73 (3) ◽  
pp. 457-463 ◽  
Author(s):  
K. Gorlach-Lira ◽  
C. Pacheco ◽  
L.C.T. Carvalho ◽  
H.N. Melo Júnior ◽  
M.C. Crispim

This work was carried out to analyse the microbiological parameters of the water quality of a reservoir used for the irrigation and culture of tilapia (Oreochromis niloticus) in floating net cages. The physico-chemical parameters, counts of mesophilic total aerobic bacteria, total and thermotolerant coliforms and fecal streptococci, and the presence of Escherichia coli in samples of water collected in three sites of the reservoir (pre-culture site, culture site, post-culture site) were analysed. The levels of ammonia (0.047-0.059 mg/L), nitrite (0.001-0.021 mg/L) and total phosphorus (0.050-0.355 mg/L) in the water did not show significant differences (p > 0.05) between sampling sites. The levels of total bacteria in the water varied between 1.3 x 104 and 67.3 x 104 CFU/100 mL. The MPN values of thermotolerant coliforms (< 930 MPN/100 mL) were within values recommended for water used for fish culture and/or irrigation. The presence of E. coli and fecal streptococci were verified in 48% and 56% of analysed samples, respectively. The site with floating net cages showed more samples contaminated with E. coli and fecal streptococci than other sampling points.


2022 ◽  
Vol 17 (12) ◽  
Author(s):  
Ana Carolina Almeida de Oliveira Ferreira ◽  
Erika da Silva Monteiro ◽  
David de Oliveira Sousa ◽  
Calliandra Maria de Souza Silva ◽  
Izabel Cristina Rodrigues Da Silva ◽  
...  

Tilapia (Oreochromis niloticus) is the most cultivated and consumed freshwater fish in Brazil. The present study aimed to evaluate the microbiological quality of ice and fresh tilapia samples commercialized in the Federal District. Tilapia samples were tested for counts of mesophilic and psychrotrophic bacteria, determination of total and thermotolerant coliforms, Staphylococcus aureus counts and presence of Salmonella. Ice samples were analyzed for determination of total coliforms and thermotolerant coliforms and presence of Escherichia coli. Of the 20 samples of fresh tilapia analyzed, ten samples (50%) presented Salmonella (genetically confirmed through the presence of the invA gene) and, therefore, were unfit for consumption. S. aureus was found in 11 samples (55%), and one sample of fillet presented S. aureus counts (3.15 CFU/g) above the limit allowed by Brazilian legislation (3 log CFU/g). S. aureus colonies were confirmed by detection of CoA gene in molecular analysis. Of the 14 ice samples analyzed, 12 samples (85.7%) were unfit for use in fish conservation due to the presence of total coliforms and 9 ice samples (64.3%) were also contaminated with thermotolerant coliforms. E. coli was isolated from 6 ice samples (42.9%) and confirmed in the molecular analysis through the amplification of the MalB gene. In conclusion, the high contamination of tilapia samples with Salmonella and of the ice used for its conservation with coliforms and E. coli indicates the need for better hygienic practices in the tilapia production chain, to increase its quality and microbiological safety.


Author(s):  
Julia Derx ◽  
Jürgen Komma ◽  
Philipp Reiner ◽  
Julia Vierheilig ◽  
Domenico Savio ◽  
...  

AbstractThe alluvial backwater areas of the Danube are valuable ecological habitats containing important drinking water resources. Due to the river regulation and the construction of power plants, the river water levels and natural dynamics of the backwater areas continuously decline, threatening their typical characteristics. The aim of this study was to evaluate how an increased connectivity of the backwater branch located in a nature-protected riverine floodplain (enabled by diverting river water into the backwater system via a weir) affects the microbiological quality of groundwater resources. The defined quality criterion was that the diversion measures must not lead to an increased detection frequency of faecal indicators in groundwater. The microbiological water quality of the Danube, its backwater branch and the groundwater was analysed from 2010 to 2013. E. coli was selected as bacterial indicator for recent faecal pollution. C. perfringens (spores) was analysed as indicator for persistent faecal pollution and potentially occurring pathogenic protozoa. We simulated the microbial transport from the Danube and the backwater river into groundwater using a 3‑D unsaturated-saturated groundwater model coupled with 2‑D hydrodynamic flow simulations. Scenarios for no diversion measures were compared with scenarios for an additional discharge of 3, 20 and 80 m3/s from the Danube River into the backwater branch. While the additional discharge of 20 and 80 m3/s of Danube water into the floodplain strongly improved the ecological status according to ecological habitat models, the hydraulic transport simulations showed that this would result in a deterioration of the microbiological quality of groundwater resources. The presented approach shows how hydraulic transport modelling and microbiological analyses can be combined to support decision-making.


Author(s):  
Nurcholis Salman

The rivers flowing through the City of Tasikmalaya include the Citanduy River, the Ciloseh River, the Ciwulan River and the Cibanjaran River. While the tributaries are the Cibanjaran River which include the Cihideung/ Dalem Suba River, the Cipedes River, the Ciromban River, the Cidukuh River, the Cicacaban River, the Cibadodon River, the Cikalang River, the Tonggong Londok River, the Cibeureum River and the Cimulu River. These rivers flow and empty into the Citanduy River. The aims and objectives of this study are environmental quality monitoring activities, especially the quality of river water in the Tasikmalaya City. To find out in more detail and carefully the level of pollution that occurs, this study aims to conduct water quality monitoring so that the resulting data can determine the source of pollutants that cause deterioration of river water quality. Monitoring was conducted during the rainy season and the dry season. Based on the monitoring, it appears that the river being monitored does not meet the quality standards as a class II water body with varying status ranging from lightly polluted to heavily polluted. Parameters that do not meet quality standards include TSS, BOD, COD, nitrite, Cr6+, Zn, free chlorine, Total Phosphate, oil and fat, E. Coli and Total Coliform.


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