Immobilization of Papain on Flexible Magnetic Nanoparticles

2015 ◽  
Vol 723 ◽  
pp. 511-514
Author(s):  
Lian Tan ◽  
Lan Qian Li ◽  
Jing Dong ◽  
Zu Lan Liu ◽  
Yi Ping Liu ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Biological Activity ◽  
Magnetic Nanoparticles ◽  
Potential Application ◽  
Magnetic Particles ◽  
Immobilized Enzyme ◽  
Dialdehyde Starch ◽  
Medium Ph ◽  
Food Industries ◽  
Negative Effect

Flexible magnetic nanoparticles decorated with dialdehyde starch (DAS) were developed and used as a novel enzyme support for the covalent conjugation of papain. The analyses of Fourier transform infrared (FTIR) spectroscopy confirmed the preparation of magnetic particles with flexible long molecular chains on their surfaces and conjugation of papain with the Fe3O4-DAS nanoparticles. Considering that the immobilized papain was found to exhibit better tolerance to the variations of temperature and medium pH, an advantage of easy to magnetic separation and lack of negative effect on biological activity, the kind of flexible magnetic bioconjugate support should be a good immobilized enzyme carrier, and has potential application in textile, leather, food industries.

Polymer grafted recyclable magnetic nanoparticles

2015 ◽  
Vol 6 (2) ◽  
pp. 248-255 ◽  
Author(s):  
Lei Wang ◽  
Marcus Cole ◽  
Junting Li ◽  
Yang Zheng ◽  
Yung Pin Chen ◽  
...  
Keyword(s):  
Biological Activity ◽  
Magnetic Nanoparticles ◽  
Aqueous Phase ◽  
Methacrylic Acid ◽  
Magnetic Particles ◽  
High Biological Activity ◽  
Water Solutions ◽  
Biological Environment

The recyclable poly(methacrylic acid) grafted magnetic particles retained excellent aqueous phase dispersibility and high biological activity against bacteria when loaded with an antibiotic. The particles were removed from water solutions using a magnet after antimicrobial testing, thus avoiding nano-based pollution of the biological environment.

Useful Oriented Immobilization of Antibodies on Chimeric Magnetic Particles: Direct Correlation of Biomacromolecule Orientation with Biological Activity by AFM Studies

Langmuir ◽  
2014 ◽  
Vol 30 (49) ◽  
pp. 15022-15030 ◽  
Author(s):  
Marzia Marciello ◽  
Marco Filice ◽  
David Olea ◽  
Marisela Velez ◽  
José M. Guisan ◽  
...  
Keyword(s):  
Biological Activity ◽  
Magnetic Particles ◽  
Oriented Immobilization

scholarly journals Removal of Emulsified Oil from Aqueous Environment by Using Polyvinylpyrrolidone-Coated Magnetic Nanoparticles

Water ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1993 ◽  
Author(s):  
Shao ◽  
Li ◽  
Lü ◽  
Qi ◽  
Zhang ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Surfactant Concentration ◽  
Solvothermal Method ◽  
Aqueous Environment ◽  
Food Industries ◽  
Effects Of Ph ◽  
One Step ◽  
Oily Wastewater Treatment ◽  
Neutral Conditions ◽  
Demulsification Efficiency

In recent years, a large amount of emulsified oily wastewaters were produced from petroleum and food industries, resulting in severe environmental problems. In this study, a series of polyvinylpyrrolidone (PVP)-coated Fe3O4 magnetic nanoparticles (MNPs) were prepared via one-step solvothermal method by introducing various amounts or types of PVP. The synthesized MNPs were characterized by multiple techniques, and their demulsification performances were evaluated in petrochemical and vegetable oil wastewaters, respectively. Results showed that the introduction of PVP in solvothermal process could significantly enhance the demulsification efficiency of MNPs, although excessive addition of PVP could not further increase its efficiency. Moreover, the effects of pH, surfactant concentration of wastewater, and the recycle number of MNPs on the demulsification performance were investigated in detail. It was found that the demulsification efficiency decreased with the increase of pH and surfactant concentration, and the synthetic MNPs were still effective after being reused for 5 cycles under acidic and neutral conditions. It is expected that the development of the PVP-coated MNPs can provide a simple and powerful route for the oily wastewater treatment.

Synthesis and Biological Activity of Hybrid Systems of Magnetic Nanoparticles with Antibacterial Drugs

2019 ◽  
Vol 74 (6) ◽  
pp. 322-325
Author(s):  
O. I. Vernaya ◽  
A. S. Shumilkin ◽  
V. P. Shabatin ◽  
T. I. Shabatina ◽  
M. Ya. Melnikov
Keyword(s):  
Biological Activity ◽  
Magnetic Nanoparticles ◽  
Hybrid Systems ◽  
Antibacterial Drugs

Adsorption Characteristics of Hg2+ Ions Using Fe3O4/Chitosan Magnetic Nanoparticles

2011 ◽  
Vol 291-294 ◽  
pp. 72-75 ◽  
Author(s):  
Bo Lin Liang
Keyword(s):  
Magnetic Nanoparticles ◽  
Adsorption Capacity ◽  
Uniform Distribution ◽  
Ph Value ◽  
Cross Linking ◽  
Mercury Ions ◽  
Dialdehyde Starch ◽  
Adsorption Time ◽  
Adsorption Performance ◽  
Chemical Cross Linking

Fe3O4/chitosan magnetic nanoparticles were prepared by chemical cross-linking technique, using dialdehyde starch as a crosslinker, and the adsorption performance of the adsorbent toward Hg2+ was investigated. The adsorption capacity of mercury ions was greatly affected by pH value, adsorption doses and adsorption time. Results indicated that optimal adsorption conditions were pH value, 6, adsorption doses, 140 mg.L-1, adsorption time, 20 min, and the adsorption capacity was 25.12 mg.g-1. The well-defined nanoparticles with a uniform distribution were observed.

Field-induced anti-nematic and biaxial ordering in binary mixtures of discotic mesogens and spherical magnetic nanoparticles

Soft Matter ◽  
2020 ◽  
Vol 16 (47) ◽  
pp. 10667-10675
Author(s):  
Stavros D. Peroukidis ◽  
Sabine H. L. Klapp ◽  
Alexandros G. Vanakaras
Keyword(s):  
Magnetic Nanoparticles ◽  
Binary Mixtures ◽  
Computer Simulations ◽  
Liquid Crystalline ◽  
Magnetic Particles ◽  
Equilibrium Structure ◽  
Discotic Mesogens ◽  
External Stimuli

Using computer simulations we explore the equilibrium structure and response to external stimuli of complex magnetic hybrids consisting of magnetic particles in discotic liquid crystalline matrices.

scholarly journals Epimeric Mixtures of Brassinosteroid Analogs: Synthesis, Plant Growth, and Germination Effects in Tomato (Lycopersicum esculentum Mill.)

Agronomy ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 808
Author(s):  
Nitza Soto ◽  
César González ◽  
Marco Mellado ◽  
Andrés F. Olea ◽  
Yamilet Coll ◽  
...  
Keyword(s):  
Biological Activity ◽  
Plant Growth ◽  
Potential Application ◽  
Growth And Development ◽  
Side Chains ◽  
Lycopersicum Esculentum ◽  
Good Level ◽  
Hyodeoxycholic Acid ◽  
Pure Compounds

Brassinosteroids (BRs) play an important role in the growth and development of plants. Herein, we describe the synthesis of epimeric mixtures of BR analogs with 24-norcholane type side chains, S/R configuration at C22 and A/B ring cis-type fusion. All epimeric mixtures were synthetized from hyodeoxycholic acid. The biological activity of mixtures was evaluated by using rice lamina inclination test and germination of tomato (Lycopersicum esculentum) seeds. The results show that these epimeric mixtures exhibit similar bioactivity to brassinolide in both bioassays. Thus, our results corroborate that the A/B junction has almost no effect on bioactivity and open the possibility of using epimeric mixtures instead of pure compounds. In this approach, the synthesized BR analogs maintain a good level of bioactivity, whereas the synthesis is shorter, cheaper and with higher yields. All these factors make this alternative very interesting for potential application.

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