Incomplete Nucleic Acid Sequences Visualization: A Case Study in Virus Sequences

2015 ◽  
Vol 781 ◽  
pp. 637-640
Author(s):  
Thitiwat Piyatamrong ◽  
Anan Kamolphanus ◽  
Gasydech Lergchinnaboot ◽  
Krittin Suphakarn ◽  
Chivalai Temiyasathit
Keyword(s):  
Nucleic Acid ◽  
Dengue Virus ◽  
East Asian ◽  
Majority Voting ◽  
Genome Sequences ◽  
The World ◽  
Database Technology ◽  
Virus Sequence ◽  
Reference Sequences

Dengue virus (DENV) is one of the most widespread infectious diseases in the world, especially in the South East Asian regions. Transmitting the virus through mosquitoes, Dengue is an infectious viral borne disease. The virus sequences are assembled as series of nucleic acid, making the task of diagnosing virus sequences burdensome. Graphical representations are then proposed to represent Dengue virus to sustain the studies in virus sequences diagnosis. However, graphically representing sequences remained a crucified task especially for the incomplete genome sequences due to the missing nucleic acids. Although a number of studies provide methodologies on virus sequence visualization, in Dengue virus researches, those methodologies provide the visualization solely for complete genome sequences while neglecting the incomplete genome sequences. With the unaccommodating availabilities of research inputs, our study proposes a methodology for graphically representing the incomplete Dengue virus sequences, as well as complete virus sequences, by imputing in the incomplete part of a sequence with created reference sequences. The proposed methodology employs the use of database technology and majority voting technique to create reference sequences for each serotype of Dengue. Experimental results show that incomplete sequences are visualized realistically according to its respective serotype, thus providing flexibilities in Dengue virus researches to compensate incomplete sequences as inputs.

scholarly journals Complete Genome Sequences of Dengue Virus Type 1 to 4 Strains Used for the Development of CBER/FDA RNA Reference Reagents and WHO International Standard Candidates for Nucleic Acid Testing

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Germán Añez ◽  
Daniel A. Heisey ◽  
Evgeniya Volkova ◽  
Maria Rios
Keyword(s):  
Nucleic Acid ◽  
Dengue Virus ◽  
Nucleic Acid Testing ◽  
Genome Sequences ◽  
International Standard ◽  
Denv Serotypes ◽  
The World ◽  
Complete Sequences ◽  
Clinically Significant

Dengue virus (DENV), a member of the Flaviviridae family, is the most common and clinically significant arbovirus in the world and is endemic in more than 100 countries. Here, we report the complete sequences of four DENV serotypes used in the development of the CBER/FDA RNA reference reagents and WHO International Standard candidates for nucleic acid testing.

scholarly journals High throughput sequencing based detection of SARS-CoV-2 prevailing in wastewater of Pune, West India

2021 ◽  
Author(s):  
Tanmay Dharmadhikari ◽  
Vinay Rajput ◽  
Rakeshkumar Yadav ◽  
Radhika Boargaonkar ◽  
Dayanand Panse ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Clinical Data ◽  
High Throughput Sequencing ◽  
Novel Mutation ◽  
Indicator System ◽  
Valuable Resource ◽  
The World ◽  
Clinical Detection ◽  
Asymptomatic Individuals

Given a large number of SARS-CoV-2 infected individuals, clinical detection has proved challenging. The wastewater-based epidemiological paradigm would cover the clinically escaped asymptomatic individuals owing to the faecal shedding of the virus. We hypothesised using wastewater as a valuable resource for analysing SARS-CoV-2 mutations circulating in the wastewater of Pune region (Maharashtra; India), one of the most affected during the covid-19 pandemic. We conducted a case study in open wastewater drains from December 2020-March 2021 to assess the presence of SARS-CoV-2 nucleic acid and further detect mutations using ARTIC protocol of MinION sequencing. The analysis revealed 108 mutations across six samples categorised into 40 types of mutations. We report the occurrence of mutations associated with B.1.617 lineage in March-2021 samples, simultaneously also reported as a Variant of Concern (VoC) responsible for the rapid increase in infections. The study also revealed four mutations; S:N801, S:C480R, NSP14:C279F and NSP3:L550del not currently reported from wastewater or clinical data in India but reported in the world. Further, a novel mutation NSP13:G206F mapping to NSP13 region was observed from wastewater. Notably, S:P1140del mutation was observed in December 2020 samples while it was reported in February 2021 from clinical data, indicating the instrumentality of wastewater data in early detection. This is the first study in India to conclude that wastewater-based epidemiology to identify mutations associated with SARS-CoV-2 virus from wastewater as an early warning indicator system.

Culture and the plasticity of perception

2020 ◽  
Vol 43 ◽  
Author(s):  
Michael Lifshitz ◽  
T. M. Luhrmann
Keyword(s):  
Cultural Context ◽  
Sensory Experience ◽  
Cultural Learning ◽  
The World

Abstract Culture shapes our basic sensory experience of the world. This is particularly striking in the study of religion and psychosis, where we and others have shown that cultural context determines both the structure and content of hallucination-like events. The cultural shaping of hallucinations may provide a rich case-study for linking cultural learning with emerging prediction-based models of perception.

HIPEREMESIS GRAVIDARUM PADA IBU HAMIL DI KLINIK BIDAN SUPRIYATI SRIBIT BERBAH SLEMAN

2019 ◽  
Vol 14 (1) ◽  
pp. 51
Author(s):  
Pramukti Dian Setianingrum ◽  
Farah Irmania Tsani
Keyword(s):  
Blood Pressure ◽  
Pregnant Women ◽  
Hyperemesis Gravidarum ◽  
Unwanted Pregnancy ◽  
The Body ◽  
Nausea And Vomiting ◽  
Daily Activities ◽  
World Health ◽  
The World

Backgroud: The World Health Organization (WHO) explained that the number of Hyperemesis Gravidarum cases reached 12.5% of the total number of pregnancies in the world and the results of the Demographic Survey conducted in 2007, stated that 26% of women with live births experienced complications. The results of the observations conducted at the Midwife Supriyati Clinic found that pregnant women with hyperemesis gravidarum, with a comparison of 10 pregnant women who examined their contents there were about 4 pregnant women who complained of excessive nausea and vomiting. Objective: to determine the hyperemesis Gravidarum of pregnant mother in clinic. Methods: This study used Qualitative research methods by using a case study approach (Case Study.) Result: The description of excessive nausea of vomiting in women with Hipermemsis Gravidarum is continuous nausea and vomiting more than 10 times in one day, no appetite or vomiting when fed, the body feels weak, blood pressure decreases until the body weight decreases and interferes with daily activities days The factors that influence the occurrence of Hyperemesis Gravidarum are Hormonal, Diet, Unwanted Pregnancy, and psychology, primigravida does not affect the occurrence of Hyperemesis Gravidarum. Conclusion: Mothers who experience Hyperemesis Gravidarum feel nausea vomiting continuously more than 10 times in one day, no appetite or vomiting when fed, the body feels weak, blood pressure decreases until the weight decreases and interferes with daily activities, it is because there are several factors, namely, hormonal actors, diet, unwanted pregnancy, and psychology.

Social Design for Services Framework

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Melanie SARANTOU ◽  
Satu MIETTINEN
Keyword(s):  
South Africa ◽  
Sustainable Development ◽  
Research Group ◽  
Design Research ◽  
Service Design ◽  
Social Design ◽  
Practical Action ◽  
The Social ◽  
The World

This paper addresses the fields of social and service design in development contexts, practice-based and constructive design research. A framework for social design for services will be explored through the survey of existing literature, specifically by drawing on eight doctoral theses that were produced by the World Design research group. The work of World Design researcher-designers was guided by a strong ethos of social and service design for development in marginalised communities. The paper also draws on a case study in Namibia and South Africa titled ‘My Dream World’. This case study presents a good example of how the social design for services framework functions in practice during experimentation and research in the field. The social design for services framework transfers the World Design group’s research results into practical action, providing a tool for the facilitation of design and research processes for sustainable development in marginal contexts.

Drowning in negativism, self-hate, doubt, madness: Linguistic insights into Sylvia Plath’s experience of depression

2015 ◽  
Vol 11 (1) ◽  
pp. 41-54 ◽  
Author(s):  
Zsófia Demjén
Keyword(s):  
Lived Experience ◽  
Medical Humanities ◽  
Sylvia Plath ◽  
Individual Experience ◽  
Qualitative And Quantitative ◽  
The World ◽  
The Individual ◽  
Linguistic Methods ◽  
Linguistic Approach

This paper demonstrates how a range of linguistic methods can be harnessed in pursuit of a deeper understanding of the ‘lived experience’ of psychological disorders. It argues that such methods should be applied more in medical contexts, especially in medical humanities. Key extracts from The Unabridged Journals of Sylvia Plath are examined, as a case study of the experience of depression. Combinations of qualitative and quantitative linguistic methods, and inter- and intra-textual comparisons are used to consider distinctive patterns in the use of metaphor, personal pronouns and (the semantics of) verbs, as well as other relevant aspects of language. Qualitative techniques provide in-depth insights, while quantitative corpus methods make the analyses more robust and ensure the breadth necessary to gain insights into the individual experience. Depression emerges as a highly complex and sometimes potentially contradictory experience for Plath, involving both a sense of apathy and inner turmoil. It involves a sense of a split self, trapped in a state that one cannot overcome, and intense self-focus, a turning in on oneself and a view of the world that is both more negative and more polarized than the norm. It is argued that a linguistic approach is useful beyond this specific case.

The World Can Be Flat: Case Study of Flat-Rate Pricing for Vanpool Operations

2006 ◽  
Vol 1956 ◽  
pp. 30-33 ◽  
Author(s):  
David Ungemah ◽  
Malaika Rivers ◽  
Stuart Anderson
Keyword(s):  
Flat Rate ◽  
The World

Flexible Epistemologies: Gypsy/roma Thinking and Anthropology Theory

2020 ◽  
Vol 24 (2) ◽  
pp. 228-240
Author(s):  
Stefania Pontrandolfo ◽  
Marco Solimene
Keyword(s):  
Cultural Construction ◽  
The Social ◽  
The World ◽  
Specific Practice

This article reflects on the conceptual debt that anthropology has developed towards the peoples it studies, by exploring the case-study of Gypsy/Roma anthropology. We argue that ethnographically-grounded research has enabled anthropologists to access and incorporate Gypsy/Roma visions and practices of the world. The flexible Gypsy epistemologies, which Gypsies/ Roma use in the social and cultural construction of particular forms of identity and mobility, have thus translated into a specific practice of theory, which has provided more adequate tools for grasping the complexity of reality and contributed to a decolonialisation of anthropological thought.

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