Isolation and Characterization of Rice Prolamin

2011 ◽  
Vol 365 ◽  
pp. 187-193
Author(s):  
Xin Xin Song ◽  
Xian Ming Zhao ◽  
Ya Nan Li ◽  
Xue Peng ◽  
Shu Hong Sun ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Rice Flour ◽  
Extraction Condition ◽  
Yield Efficiency ◽  
Isolation And Characterization ◽  
Sds Page ◽  
Optimum Extraction ◽  
Optimum Extraction Condition

Rice prolamin, constituting 10, 13 and 16 kDa polypeptides, is indigestible and may work as a kind of ‘resistant protein’. To investigate the effective method for isolation of prolamin, in this study, prolamin fractions were extracted from defatted rice flour by 70% ethanol, 55% and 60% n-propanol, 55%, 60% and 100% ethylene glycol, and 60% isopropanol, respectively. The isolated prolamin fractions were compared and characterized by yield, efficiency of extraction and SDS-PAGE patterns. Our results indicated that the optimum extraction condition for isolation of prolamin was 55% n-propanol, with 13 kDa and 16 kDa as the dominant fractions in prolamin.

Optimization of Extraction Conditions of Essential Oil from Latifolia miq Roots and Stems with Supercritical CO2 Fluid

2013 ◽  
Vol 726-731 ◽  
pp. 4512-4516
Author(s):  
Ren Yong Gu ◽  
Zhi Ping Li
Keyword(s):  
Essential Oil ◽  
Optimum Condition ◽  
Raw Materials ◽  
Extraction Process ◽  
Orthogonal Test ◽  
Single Factor ◽  
Extraction Condition ◽  
Optimum Extraction ◽  
Optimum Extraction Condition

Studying on the extraction process of essential oil from Latifolia Miq roots and stems by Supercritical CO2 extraction. The effects on extracting ratio were investigated by using single factor test, adding content of grinding fineness of raw materials,extracting temperature, extracting pressure and extracting time. The optimum extraction condition was determined by orthogonal test. The results showed that the optimum condition of the extraction was as follows: grinding fineness 90 mesh, extracting temperature 45 °C, extracting pressure 20 MPa and extracting time 2.5 h.

Extraction of Polyethylene Residue Components in Soil

2012 ◽  
Vol 724 ◽  
pp. 65-68
Author(s):  
Cheng Chen ◽  
Xue Gang Luo ◽  
Xiao Li Xiong ◽  
Xiao Yan Lin
Keyword(s):  
Molecular Weight ◽  
Soil Type ◽  
Average Molecular Weight ◽  
Extraction Ratio ◽  
Extraction Time ◽  
Extraction Condition ◽  
The Times ◽  
Optimum Extraction ◽  
Total Extraction ◽  
Optimum Extraction Condition

The extraction of polyethylene residue components in soil was presented in this work, where polyethylene (PE) powder (Mw=5598) was taken as a simulated polyethylene residue, and decalin was as an excellent solvent. The effects of times of reflux extraction, total extraction time and soil type on the extraction ratio were investigated, aimed at finding out the optimum extraction condition. Results show that the optimum extraction ratio achieves up to 90 % (w/w) at 100 after extraction for 20 mins thrice. Meanwhile, the times of reflux extraction has the most significant influence on extraction ratio, followed by the total extraction time. However, soil type has little influence. The FTIR spectra illustrates that the structure of PE is not destroyed during the extraction. However, the molecular weight and molecular weight distribution test show the average molecular weight of PE decreases after the extraction, which is possibly caused by the degradation of PE.

scholarly journals CENTRAL COMPOSITE DESIGN FOR OPTIMIZING EXTRACTION OF EGCG FROM GREEN TEA LEAF (CAMELLIA SINENSIS L.)

2018 ◽  
Vol 10 (6) ◽  
pp. 211
Author(s):  
Eka Indra Setyawan ◽  
Erna Prawita Setyowati ◽  
Abdul Rohman ◽  
Akhmad Kharis Nugroho
Keyword(s):  
Water Temperature ◽  
Green Tea ◽  
Epigallocatechin Gallate ◽  
Extraction Yield ◽  
Hot Water ◽  
Extraction Condition ◽  
Optimum Extraction ◽  
Central Composite ◽  
Brewing Time ◽  
Optimum Extraction Condition

Objective: This study was intended to optimize the extraction condition using central composite design.Methods: Central composite cesign with three independent variables, namely water temperature, brewing time, and brewing number were used to obtain the optimum extraction condition. Two dependent variables, namely yield of extraction and epigallocatechingallate level were used as a response parameter. Epigallocatechin gallate level was determined by using high-performance liquid chromatography method.Results: Extraction yield was varied from 0.30 g to 0.72 g. All variables, namely water temperature, brewing time, and brewing number were able to increase the extraction yield. Epigallocatechingallate level was varied from 190.23 mg/g to 301.74 mg/g. Water temperature, brewing time, and both interaction were able to increase the epigallocatechin gallate level in green tea extract.Conclusion: Optimum extraction condition was shown using hot water at a temperature of 95 °C for 20 min and two-times infusions. The condition obtained extraction yield and epigallocatechingallate of 0.70 g and 286.87 mg/g dry weight, respectively.

scholarly journals Determination of optimum extraction condition for edible oil and β-carotene from carrot peel (Daucus carota L.) using supercritical carbon dioxide

2019 ◽  
Vol 18 (2) ◽  
pp. 97-115
Author(s):  
Suthida Akkarachaneeyakorn ◽  
Tanasith Tangpaiboonpongsa ◽  
Mintra Chuakam ◽  
Kwanchanok Dasiri ◽  
Pattama Phasuthan ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Daucus Carota ◽  
Edible Oil ◽  
Extraction Condition ◽  
Daucus Carota L ◽  
Optimum Extraction ◽  
Β Carotene ◽  
Optimum Extraction Condition

scholarly journals Isolation and characterization of high affinity and highly stable anti-Chikungunya virus antibodies using ALTHEA Gold Libraries™

2020 ◽  
Author(s):  
Martha Pedraza-Escalona ◽  
Omar Guzmán-Bringas ◽  
Ivan Arrieta-Oliva ◽  
Keyla Gómez-Castellano ◽  
Juana Salinas-Trujano ◽  
...  
Keyword(s):  
Chikungunya Virus ◽  
Dynamic Range ◽  
Sandwich Elisa ◽  
Diagnostic Methods ◽  
Diagnostic Tools ◽  
Chikv Infection ◽  
High Affinity ◽  
Isolation And Characterization ◽  
Sds Page

Abstract Background More than three million infections were attributed to Chikungunya virus (CHIKV) in the 2014–2016 outbreak in Mexico, Central and South America, with over 500 deaths directly or indirectly related to this viral disease. CHIKV outbreaks are recurrent and no vaccine nor approved therapeutics exist to prevent or treat CHIKV infection. Reliable and robust diagnostic methods are thus critical to control future CHIKV outbreaks. Direct CHIKV detection in serum samples via highly specific and high affinity anti-CHIKV antibodies has shown to be an early and effective clinical diagnosis. Methods Chikungunya virions isolated from serum of a patient in Veracruz, México, were purified and characterized via electron microscopy, SDS-PAGE and binding to diverse well-characterized anti-CHIKV monoclonal antibodies. UV-inactivated CHIKV particles were used as selector in a solid-phase panning coupled with ELISA-based screening and Next-Generation Sequencing to discover specific and high affinity anti-CHIKV antibodies from ALTHEA Gold Libraries™. Results The CHIKV isolate showed the typical morphology of the virus. Protein bands in the SDS-PAGE were consistent with the size of its capsid proteins. UV-inactivated CHIKV particles bound tightly the control antibodies. The lead antibodies here obtained showed high expression yield, monomeric content over 95% after a single-step Protein A purification, and importantly, a thermal stability above 75oC. Most of the antibodies recognized linear epitopes on E2, including the highest affinity antibody called C7. A sandwich ELISA implemented with C7 and a potent neutralizing antibody isolated elsewhere, also specific for E2 but recognizing a discontinuous epitope, showed a dynamic range of 0.2–40.0 µγ/mL of UV-inactivated CHIKV purified preparation. The number of CHIKV particles estimated based on the concentration of E2 in the extract suggested that the assay could detect clinically meaningful amounts of CHIKV in serum. Conclusions The newly discovered antibodies offer valuable tools for characterization of CHIKV isolates and development of robust diagnostic tools for CHIKV infection surveillance. Application of ALTHEA Gold Libraries™ in combination with viral particles other than CHIKV could expedite the discovery and development of antibodies for detection and control of emergent and quickly spreading viral outbreaks such as SARS-CoV-2 (COVID-19).

Isolation and Characterization of Viridin, a New 65 kDa Antifungal Protein from the Mould Trichoderma viride

1999 ◽  
Vol 380 (10) ◽  
pp. 1243-1245 ◽  
Author(s):  
Jian-Jiang Hao ◽  
Chuan-dong Geng ◽  
Wei-jun Xie ◽  
Zhen-zhen Gong ◽  
Wang-Yi Liu ◽  
...  
Keyword(s):  
High Performance ◽  
Culture Medium ◽  
Basic Protein ◽  
Trichoderma Viride ◽  
Exchange Chromatography ◽  
Antifungal Protein ◽  
Cation Exchange Chromatography ◽  
Isolation And Characterization ◽  
Sds Page

AbstractA new extracellular antifungal protein with a yield of 10 mg per liter was isolated from the culture medium of the mouldTrichoderma viride. The protein, which we named viridin, was purified by carboxymethyl-cellulose cation-exchange chromatography and Superose 12 HR 10/30 high-performance liquid chromatography. Viridin, a basic protein of approximately 65 kDa as determined by SDS-PAGE, inhibits the growth of the cotton pathogenVerticillum dahliae, the IC50being 6 ΜM.

scholarly journals Isolation and Characterization of Phages InfectingBacillus subtilis

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Anna Krasowska ◽  
Anna Biegalska ◽  
Daria Augustyniak ◽  
Marcin Łoś ◽  
Malwina Richert ◽  
...  
Keyword(s):  
Food Production ◽  
Burst Size ◽  
Industrial Applications ◽  
Isolation And Characterization ◽  
Latent Time ◽  
Food Borne ◽  
Alternative Approach ◽  
Sds Page

Bacteriophages have been suggested as an alternative approach to reduce the amount of pathogens in various applications. Bacteriophages of various specificity and virulence were isolated as a means of controlling food-borne pathogens. We studied the interaction of bacteriophages withBacillusspecies, which are very often persistent in industrial applications such as food production due to their antibiotic resistance and spore formation. A comparative study using electron microscopy, PFGE, and SDS-PAGE as well as determination of host range, pH and temperature resistance, adsorption rate, latent time, and phage burst size was performed on three phages of theMyoviridaefamily and one phage of theSiphoviridaefamily which infectedBacillus subtilisstrains. The phages are morphologically different and characterized by icosahedral heads and contractile (SIOΦ, SUBω, and SPOσphages) or noncontractile (ARπphage) tails. The genomes of SIOΦ and SUBωare composed of 154 kb. The capsid of SIOΦ is composed of four proteins. Bacteriophages SPOσand ARπhave genome sizes of 25 kbp and 40 kbp, respectively. Both phages as well as SUBωphage have 14 proteins in their capsids. Phages SIOΦ and SPOσare resistant to high temperatures and to the acid (4.0) and alkaline (9.0 and 10.0) pH.

scholarly journals Isolation and characterization of circumferential microfilament bundles from retinal pigmented epithelial cells.

1982 ◽  
Vol 95 (1) ◽  
pp. 310-315 ◽  
Author(s):  
K Owaribe ◽  
H Masuda
Keyword(s):  
Epithelial Cells ◽  
Electrophoretic Pattern ◽  
Isolation And Characterization ◽  
Microfilament Bundles ◽  
Myosin Subfragment ◽  
Sds Page ◽  
Myosin Subfragment 1 ◽  
Retinal Pigmented Epithelial Cells ◽  
Large Contraction

Chicken retinal pigmented epithelial cells have circumferential microfilament bundles (CMBs) at the zonula adherens region. We have isolated these CMBs in intact form and characterized them structurally and biochemically. Pigmented epithelia obtained from 11-d-old chick embryos were treated with glycerol and Triton. Then, the epithelia were homogenized by passing them through syringe needles. Many isolated CMBs were found in the homogenate by phase-contrast microscopy. They formed polygons, mostly pentagons and hexagons, or fragments of polygons. Polygons were filled with meshwork structures, i.e. they were polygonal plates. Upon exposure to Mg-ATP, isolated CMBs showed clear and large contraction. The contraction was inhibited by treatment with N-ethylmaleimide-modified myosin subfragment-1. After purification by centrifugation with the density gradient of Percoll, CMBs were analyzed by SDS PAGE. The electrophoretic pattern gave three major components of 200, 55, and 42 kdaltons and several minor components. Electron microscopy showed that the polygons were composed of thick bundles of actin-containing microfilaments, and the meshworks were composed primarily of intermediate filaments.

scholarly journals Studied on the extraction, response surface analysis and antioxidant activity of flavonoids in the Momordia cochinchinensis seeds

2018 ◽  
Vol 238 ◽  
pp. 04004
Author(s):  
Junyu Jiang ◽  
Aiping Fei ◽  
Yong Wang ◽  
Zhigang Fang ◽  
Yingxue Teng ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Response Surface ◽  
Surface Analysis ◽  
Ethanol Concentration ◽  
Response Surface Analysis ◽  
Extraction Time ◽  
Average Yield ◽  
Extraction Condition ◽  
Optimum Extraction ◽  
Optimum Extraction Condition

This text was mainly studied the extraction flavonoids technology from Momordia cochinchinensis seeds. The optimum extraction condition was as follows: when the ratio of liquid to solid was 20:1, the ethanol concentration was 90% and the extraction time was 2h. Under this condition, the actual average yield of flavonoids could reach 0.363%.

scholarly journals CHARACTERIZATION OF CRUDE EXTRACT POLYPHENOLOXIDASE ENZYME FROM BLACK TIGER SHRIMP (Penaeus monodon)

2013 ◽  
Vol 5 (2) ◽  
Author(s):  
Made Suhandana ◽  
Tati Nurhayati ◽  
Laksmi Ambarsari
Keyword(s):  
Enzyme Activity ◽  
Metal Ion ◽  
Penaeus Monodon ◽  
Extraction Condition ◽  
Ph Optimum ◽  
Km Value ◽  
Shrimp Industry ◽  
Optimum Extraction ◽  
Inhibited Enzyme

<p>Shrimp is a  very important export  commodity with  high market value world wide. However, it is still facing problem related to the waste and deterioration quality as main issues for the shrimp industry. In this experiment, polyphenoloxidase from the carapace of Penaeus monodon was extracted and characterized. The research was carried out to obtain the optimum extraction condition and to evaluate the properties of enzyme i.e., pH, optimum temperature for activating enzyme, kinetic enzyme, and chelating on metal ion. The best method for PPO enzyme extraction used buffer with 1:3  proportion.  The optimum activity of enzyme was at pH 7 and temperature of 35°C. The kinematic enzyme (Km) value and the maximum substrate concentration were 5.42 mM and 7.5 mM, respectively.  Na<sup>+</sup>, Ca<sup>2+</sup>, Zn<sup>2+</sup>, and EDTA with concentration 5 and 10 mM inhibited enzyme activity.  Cu<sup>2+</sup>at concentration of 10 mM and Mn<sup>2+</sup> at concentration 5 mM also inhibited enzyme activity</p> <p>Keywords: carapace, characterization, polyphenoloxidase, shrimp</p>

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