Preparation and Properties of Multiple Layer Silk Fibroin Film Incorporating Sulfadiazine Sodium

2013 ◽  
Vol 641-642 ◽  
pp. 910-914
Author(s):  
Shao Qun Li ◽  
Yu Bin Tang ◽  
Jun Qiang Jia ◽  
Ming Zhu Jiang ◽  
Hui Yan
Keyword(s):  
Mechanical Property ◽  
Silk Fibroin ◽  
Release Rate ◽  
Good Mechanical Property ◽  
Distilled Water ◽  
Structure Information ◽  
Silk Fibroin Film ◽  
Ft Ir ◽  
Release Speed ◽  
Β Sheet

Silk fibroin film (SFF) has good mechanical property, cells compatibility and biodegradability, and was widely used in the biomedical area as controlling materials. In this work, silk fibroin (SF) was obtained from cocoons. It was dissolved in distilled water to form 5 % and 8 % (W/V) SF solution, which was used to prepare 0.5 %, 1.0 % and 2 % (W/V) sulfadiazine sodium (SD) solution. Five layers of SFF was prepared with salivation method, dried under 37 °C, and annealed with 90 % (V/V) methanol. The structure information was analyzed by FT-IR and SEM, SD release in PBS (0.2 mol/L, pH 7.4) and PBS containing 2 U/ml trypsin at 37 °C was measured. The FT-IR spectra showed that more β-sheet was formed after annealed by methanol; a lot of pores were in the SFF and SD was embedded in SFF through analyzing the image of SEM. The release speed from SFF made by 5 % SF solution was faster than that from SFF made by 8 % SF solution. As for SFF containing different concentration of SD, the release rate improved with the increase of concentration of SD, however, the time of reaching the maximum were approximate. In PBS with trypsin, the time of reaching the maximum accumulative release was one hour earlier compared with in the PBS. Therefore, we draw a conclusion that the SFF incorporating SD has good properties and it has the feasibility to be used as artificial skin for burned patients.

Fabrication of robust silk fibroin film by controlling the content of β-sheet via the synergism of Uv-light and ionic liquids

2019 ◽  
Vol 492 ◽  
pp. 55-65 ◽  
Author(s):  
Congxia Xie ◽  
Wenjing Li ◽  
Qingqing Liang ◽  
Shitao Yu ◽  
Lu Li
Keyword(s):  
Ionic Liquids ◽  
Silk Fibroin ◽  
Uv Light ◽  
Silk Fibroin Film ◽  
Β Sheet

Comparison of Porosity Improvement of Silk Fibroin Membrane Using Polyethylene Glycol and Glutaraldehyde for Increasing Oxygen Permeability

2013 ◽  
Vol 750-752 ◽  
pp. 1601-1608
Author(s):  
Pacharawan Ratanasongtham ◽  
Ruangsri Watanesk ◽  
Surasak Watanesk
Keyword(s):  
Polyethylene Glycol ◽  
Silk Fibroin ◽  
Oxygen Permeability ◽  
Porous Membrane ◽  
Swelling Behavior ◽  
Random Coil ◽  
Environmental Friendliness ◽  
Ft Ir ◽  
Water Swelling ◽  
Β Sheet

Biomaterial is one of the good candidates for porous membrane preparation according to its environmental friendliness. In this work, the porous membranes of silk fibroin (SF) were prepared by solution casting with the addition of polyethylene glycol (PEG) and glutaraldehyde (GTA) aiming to improve the porosity and oxygen permeability of SF membrane. The conformation of SF was changed from random coil to β sheet form after treatment with MeOH. The interaction existing between SF chains and both PEG and GTA were characterized using Fourier Transform Infrared spectroscopy (FT-IR). The addition of PEG could produce more porosity in the membrane than GTA confirmed by their morphology observed from scanning electron microscopy (SEM). Moreover, the swelling behavior of the SF-PEG and SF-GTA membranes depended on the porous structure of the membrane which directly correlated to their oxygen permeability. The porosity of the SF membranes increased with the increase of PEG and GTA contents up to 40% and 3%w/w, respectively. After that, their porosity decreased as seen through the SEM and water swelling results. In addition, the SF-PEG membrane turned out to have higher degrees of both porosity and oxygen permeability than the SF-GTA membrane which related to its water swelling behavior.

Effect of Gelatin on Secondary Structure, Crystallinity and Swelling Behavior of Silk Fibroin - Gelatin Hydrogels and its Application in Controlled Release of Nitrogen

2013 ◽  
Vol 750-752 ◽  
pp. 1347-1353 ◽  
Author(s):  
Atcharaporn Rattanamanee ◽  
Hataichanoke Niamsup ◽  
La-Ongnuan Srisombat ◽  
Ruangsri Watanesk ◽  
Surasak Watanesk
Keyword(s):  
Controlled Release ◽  
Secondary Structure ◽  
Silk Fibroin ◽  
Release Rate ◽  
Swelling Behavior ◽  
Fickian Diffusion ◽  
Methanol Treatment ◽  
N Release ◽  
Ft Ir ◽  
Kinetics Of

Utilization of natural polymer as biodegradable matrix for the controlled releasing fertilizer can improve the performance of the materials and make them environmentally friendly. In this work, the effect of gelatin on the properties of hydrogels was investigated. The silk fibroin (SF)-gelatin hydrogels were prepared by solvent casting and β-crystallization of SF was promoted via methanol treatment. The secondary structure and the crystallinity of the blended hydrogels were investigated using Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction analysis (XRD), respectively. Moreover, the swelling ratio of the hydrogels and also their kinetics of nitrogen (N) release were also studied. Results from the FT-IR confirmed that no intermolecular interactions had taken place between SF and gelatin. Furthermore, the increase of gelatin content in the blended hydrogels caused the decrease of the SF crystallinity detected by XRD which corresponded to the swelling behavior of the hydrogel. The release rate of nitrogen (N) depends on the composition of SF and gelatin of which its value of diffusion exponent characteristics (n) determined from the Korsmeyer-Peppas model for all of the hydrogels are smaller than 0.5. This indicates that the release of N from the hydrogels is a quasi-Fickian diffusion. Moreover, the release rate (k) and diffusion coefficient (D) of the SF-gelatin hydrogels are lower than those of the SF itself indicating a potential to use the SF-gelatin hydrogel for nitrogen controlled release application.

Effect of Gamma Irradiation on the Biocompatibility and Biodegradation of Silk Fibroin In Vivo

2012 ◽  
Vol 535-537 ◽  
pp. 2361-2364
Author(s):  
Huan Yu Jin ◽  
Yu Liu ◽  
Xing Liu ◽  
Da Peng Wang ◽  
Jian Liu ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Bone Repair ◽  
Gamma Ray ◽  
Raw Material ◽  
Good Mechanical Property ◽  
Silk Fibroin Film ◽  
Gamma Ray Irradiation ◽  
Bone Repair Material

Silk fibroin has been a raw material for many artificial biomaterials for a long term, because of it good mechanical property and fantastic biocompatibility. As a natural protein, it has more excellences than other chemically synthetical materials. In present, silk fibroin is used to manufacture bone repair material, artificial blood vessel, microcapsules and so on. However, silk fibroin-based biomaterial is not good enough in biodegradation. In our research, we exposed the silk fibroin film under the gamma ray irradiation with the doses of 25kGy and 50kGy and implanted the film subsequently on the back of SD rats. At dates (7th, 14th, 28th, 56th, 84th ) after implantation, we got the tissue with the implanted film and had the pathological analysis accordingly. The results show that the immune cells infiltration and inflammation decreased within a month. And the immune reaction decreased more quickly in the 50kGy group. And cracks of the silk fibroin film appeared earlier in the 50kGy group. From above observation, it is indicated that the silk fibroin film with a higher dose had better biocompatibility than others. And the silk biodegradation was accelerated by the higher gamma ray dose. Therefore, we can conclude that the gamma ray is able to improve the biocompatibility of silk fibroin and accelerated the biodegradation of it.

scholarly journals Chitosan hydrogel/silk fibroin/Mg(OH)2 nanobiocomposite as a novel scaffold with antimicrobial activity and improved mechanical properties

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Reza Eivazzadeh-Keihan ◽  
Fateme Radinekiyan ◽  
Hooman Aghamirza Moghim Aliabadi ◽  
Sima Sukhtezari ◽  
Behnam Tahmasebi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Cell Viability ◽  
Silk Fibroin ◽  
Analytical Techniques ◽  
Structural Features ◽  
Mechanical Tests ◽  
Chitosan Hydrogel ◽  
Biological Capacity ◽  
Ft Ir ◽  
Novel Scaffold

AbstractHerein, a novel nanobiocomposite scaffold based on modifying synthesized cross-linked terephthaloyl thiourea-chitosan hydrogel (CTT-CS hydrogel) substrate using the extracted silk fibroin (SF) biopolymer and prepared Mg(OH)2 nanoparticles was designed and synthesized. The biological capacity of this nanobiocomposite scaffold was evaluated by cell viability method, red blood cells hemolytic and anti-biofilm assays. According to the obtained results from 3 and 7 days, the cell viability of CTT-CS/SF/Mg(OH)2 nanobiocomposite scaffold was accompanied by a considerable increment from 62.5 to 89.6% respectively. Furthermore, its low hemolytic effect (4.5%), and as well, the high anti-biofilm activity and prevention of the P. aeruginosa biofilm formation confirmed its promising hemocompatibility and antibacterial activity. Apart from the cell viability, blood biocompatibility, and antibacterial activity of CTT-CS/SF/Mg(OH)2 nanobiocomposite scaffold, its structural features were characterized using spectral and analytical techniques (FT-IR, EDX, FE-SEM and TG). As well as, given the mechanical tests, it was indicated that the addition of SF and Mg(OH)2 nanoparticles to the CTT-CS hydrogel could improve its compressive strength from 65.42 to 649.56 kPa.

scholarly journals Sustained Release of Insulin-Like Growth Factor-1 from Bombyx mori L. Silk Fibroin Delivery for Diabetic Wound Therapy

2021 ◽  
Vol 22 (12) ◽  
pp. 6267
Author(s):  
Meng-Jin Lin ◽  
Mei-Chun Lu ◽  
Hwan-You Chang
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Sustained Release ◽  
Silk Fibroin ◽  
Granulation Tissue ◽  
Release Rate ◽  
Insulin Like Growth Factor ◽  
Diabetic Wound ◽  
Diabetic Mice ◽  
Wound Therapy

The goals of this study are to develop a high purity patented silk fibroin (SF) film and test its suitability to be used as a slow-release delivery for insulin-like growth factor-1 (IGF-1). The release rate of the SF film delivering IGF-1 followed zero-order kinetics as determined via the Ritger and Peppas equation. The release rate constant was identified as 0.11, 0.23, and 0.09% h−1 at 37 °C for SF films loaded with 0.65, 6.5, and 65 pmol IGF-1, respectively. More importantly, the IGF-1 activity was preserved for more than 30 days when complexed with the SF film. We show that the IGF-1-loaded SF films significantly accelerated wound healing in vitro (BALB/3T3) and in vivo (diabetic mice), compared with wounds treated with free IGF-1 and an IGF-1-loaded hydrocolloid dressing. This was evidenced by a six-fold increase in the granulation tissue area in the IGF-1-loaded SF film treatment group compared to that of the PBS control group. Western blotting analysis also demonstrated that IGF-1 receptor (IGF1R) phosphorylation in diabetic wounds increased more significantly in the IGF-1-loaded SF films group than in other experimental groups. Our results suggest that IGF-1 sustained release from SF films promotes wound healing through continuously activating the IGF1R pathway, leading to the enhancement of both wound re-epithelialization and granulation tissue formation in diabetic mice. Collectively, these data indicate that SF films have considerable potential to be used as a wound dressing material for long-term IGF-1 delivery for diabetic wound therapy.

Self-healing poly(siloxane-urethane) elastomers with remoldability, shape memory and biocompatibility

2016 ◽  
Vol 7 (47) ◽  
pp. 7278-7286 ◽  
Author(s):  
Jian Zhao ◽  
Rui Xu ◽  
Gaoxing Luo ◽  
Jun Wu ◽  
Hesheng Xia
Keyword(s):  
Mechanical Property ◽  
Shape Memory ◽  
Microphase Separation ◽  
Diels Alder ◽  
Good Mechanical Property ◽  
Self Healing ◽  
Healing Efficiency ◽  
Good Biocompatibility

The poly(siloxane-urethane) elastomers with microphase separation structure and Diels–Alder bonds show high healing efficiency, good mechanical property and good biocompatibility.

SILK FIBROIN FILMS CRYSTALLIZED BY MULTIWALLED CARBON NANOTUBES

2008 ◽  
Vol 22 (09n11) ◽  
pp. 1807-1812 ◽  
Author(s):  
H.-S. KIM ◽  
W.-I. PARK ◽  
Y. KIM ◽  
H.-J. JIN
Keyword(s):  
Electron Microscopy ◽  
Carbon Nanotubes ◽  
Multiwalled Carbon Nanotubes ◽  
Silk Fibroin ◽  
Composite Films ◽  
Tensile Modulus ◽  
Multiwalled Carbon ◽  
Regenerated Silk Fibroin ◽  
Silk Films ◽  
Β Sheet

Silk films prepared from regenerated silk fibroin are normally stabilized by β-sheet formation through the use of solvents (methanol, water etc.). Herein, we report a new method of preparing water-stable films without a β-sheet conformation from regenerated silk fibroin solutions by incorporating a small amount (0.2 wt%) of multiwalled carbon nanotubes (MWCNTs). To extend the biomaterial utility of silk proteins, forming water-stable silk-based materials with enhanced mechanical properties is essential. Scanning electron microscopy and transmission electron microscopy were used to observe the morphology of the MWCNT-incorporated silk films. The wide-angle X-ray diffraction provided clear evidence of the crystallization of the silk fibroin induced by MWCNT in the composite films without any additional annealing processing. The tensile modulus and strength of the composite films were improved by 108% and 51%, respectively, by the incorporation of 0.2 wt% of MWCNTs, as compared with those of the pure silk films. The method described in this study will provide an alternative means of crystallizing silk fibroin films without using an organic solvent or blending with any other polymers, which may be important in biomedical applications.

Fabrication and Characterization of Vitamin E TPGS Loaded Silk Fibroin/Hyaluronic Acid Nanofibrous Scaffolds

2013 ◽  
Vol 721 ◽  
pp. 274-277
Author(s):  
Li Li Ji ◽  
Qiao Ling Li ◽  
Zeng Hu Yang ◽  
Wei Jing Hu ◽  
Kui Hua Zhang
Keyword(s):  
Hyaluronic Acid ◽  
Vitamin E ◽  
Silk Fibroin ◽  
Ethanol Vapor ◽  
Random Coil ◽  
Burst Release ◽  
Nanofibrous Scaffolds ◽  
Vitamin E Tpgs ◽  
Polyethylene Glycol 1000 ◽  
Β Sheet

Vitamin E d-alpha-tocopheryl polyethylene glycol 1000 succinate (VE TPGS) loaded silk fibroin (SF)/ hyaluronic acid (HA) nanofibrous scaffolds were fabricated by means of electrospinning to biomimic the natural extracellular matrix. Scanning electronic microscopy (SEM) results indicated that electrospun VE TPGS loaded SF/HA nanofibers were ribbon-shaped, the width of nanofibers decreased slightly with the addition of VE TPGS to SF/HA blended solutions. Fourier transform infrared (FTIR) spectroscopy and Wide-angle X-ray diffraction (WAXD) curves revealed that VE TPGS did not induce SF conformation from random coil to β-sheet. SF conformation converted from random coil to β-sheet after being treated with 75% ethanol vapor. In vitro release studies confirmed VE TPGS had no obvious burst release and present good release behavior.

Mussel-inspired adhesive gelatin–polyacrylamide hydrogel wound dressing loaded with tetracycline hydrochloride to enhance complete skin regeneration

Soft Matter ◽  
2022 ◽  
Author(s):  
Yamei Wang ◽  
Dongdong Xiao ◽  
Lin Quan ◽  
Hongbin Chai ◽  
Xiaofeng Sui ◽  
...  
Keyword(s):  
Mechanical Property ◽  
Wound Dressing ◽  
Antibacterial Property ◽  
Wound Care ◽  
Skin Regeneration ◽  
Tetracycline Hydrochloride ◽  
Market Size ◽  
Good Mechanical Property ◽  
Polyacrylamide Hydrogel

Even though the global wound care market size was valued at USD 19.83 billion in 2020, it is still a challenge to develop a hydrogel-based wound dressing with a good mechanical property, adhesiveness and antibacterial property.

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