The Effects of Perfluorooctane Sulfonate (PFOS) on Proliferation of Mouse Leydig Cells In Vitro
To evaluate the male reproductive toxicity of PFOS on mammal animals at cellular level, mouse leydig cells were isolated from healthy mouse testis tissue and cultured in vitro. Adherent cells were treated with a serial concentration of PFOS for 4 more days of culture. Proliferation and DNA damage of the cells were analyzed by CCK assay and SCGE assay respectively. Forty-eight hours of treating with PFOS≧25μg/mL all inhibited the proliferation of the cells (p<0.05). PFOS seemed not to change the time for the cells to reach platform phase. DNA damage was also observed in the groups treated with PFOS dependent on dose and exposure time. The highest DNA damage level was averagely 17 cells per well in 96-well plates, which occurred to 62.5μg/mL group at 72h.