Reconstruction of Kidney-Like Tissue Using ECM Gel and Rat Embryonic Kidney Fragments

2007 ◽  
Vol 342-343 ◽  
pp. 1-4
Author(s):  
Ying Jian Zhu ◽  
Wei Liu ◽  
Wen Jie Zhang ◽  
Guang Dong Zhou ◽  
Guo Hua Liu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Subcutaneous Tissue ◽  
Kidney Tissue ◽  
Polyglycolic Acid ◽  
Negative Control ◽  
Engineering Technique ◽  
Solid Foundation ◽  
Ecm Gel ◽  
Further Development

Organ engineering remains a challenge to researchers. We tried to reconstruct kidney-like tissue using tissue engineering technique. Kidney fragments were isolated from rat E16 embryonic kidneys and seeded on either ECM gel or on polyglycolic acid (PGA) fibers, then implanted in vivo into the subcutaneous tissue of nude mice for 2 and 3 weeks, respectively. As a negative control, ECM alone was implanted. Results showed that kidney like tissue could be formed in ECMfragment constructs after 2 or 3 weeks of implantation, including the formation of glomeruli, tubules and collecting ducts. In addition, these structures seemed more mature in 3 weeks specimens than that of 2 weeks. Further development of this model may lay a solid foundation for kidney tissue engineering.

scholarly journals Antimicrobial Films Based on Nanocomposites of Chitosan/Poly(vinyl alcohol)/Graphene Oxide for Biomedical Applications

Biomolecules ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 109 ◽  
Author(s):  
Sebastián Ruiz ◽  
Julián Andrés Tamayo ◽  
Johannes Delgado Ospina ◽  
Diana Paola Navia Porras ◽  
Mayra Eliana Valencia Zapata ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Vinyl Alcohol ◽  
Biomedical Applications ◽  
Subcutaneous Tissue ◽  
Poly Vinyl Alcohol ◽  
Salmonella Spp ◽  
Mueller Hinton Agar

Today, tissue regeneration is one of the greatest challenges in the field of medicine, since it represents hope after accidents or illnesses. Tissue engineering is the science based on improving or restoring tissues and organs. In this work, five formulations of chitosan/poly(vinyl alcohol)/graphene oxide (CS/PVA/GO) nanocomposites were studied for the development of biodegradable films with potential biomedical applications. The characterization of the films consisted of Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS). The antibacterial activity was evaluated in vitro against Gram-positive bacteria Bacillus cereus and Staphylococcus aureus and Gram-negative Salmonella spp. and Escherichia coli, by contact of the film above inoculum bacterial in Müeller–Hinton agar. On the other hand, in vivo tests in which the material implanted in the subcutaneous tissue of Wistar rats demonstrated that the formulation CS/PVA/GO (14.25:85:0.75) was the best antibacterial film with adequate degradation in vivo. All together, these results indicate the potential of the films using nanocomposites of CS/PVA/GO in tissue engineering and cell regeneration.

In Vivo Evaluation of Zinc-Containing Nanostructured Carbonated Hydroxyapatite

2016 ◽  
Vol 696 ◽  
pp. 223-229 ◽  
Author(s):  
Simone Ribeiro ◽  
Suelen Cristina Sartoretto ◽  
Rodrigo Resende ◽  
Marcelo Uzeda ◽  
Adriana Terezinha Alves ◽  
...  
Keyword(s):  
Sham Group ◽  
Subcutaneous Tissue ◽  
Descriptive Analysis ◽  
Microscopic Analysis ◽  
Experimental Period ◽  
Negative Control ◽  
X Ray Diffraction ◽  
Carbonated Hydroxyapatite ◽  
Low Solubility

The hydroxyapatite (HA) is a biocompatible and bioactive biomaterial used as bone substitute, however, the high crystallinity of HA and consequently its low solubility may be a limitation for its clinical use. In order to improve the biosorption of HA, the partial substitutions in the chemical structure and doping with small amounts of impurities have been study. The objective of this study was to evaluate the biocompatibility of 3% Zinc-containing nanostructured carbonated hydroxyapatite (ZncHA) compared with the carbonated hydroxyapatite (cHA), both synthesized at 37°C and non-sintered, using as control the stoichiometric HA microspheres in subcutaneous of mice. The X-ray Diffraction (XRD) and Vibrational Spectroscopy in Infra Red Fourier Transform (FTIR) were used to characterize the biomaterials. In vivo test was performed in BALB/c mice by implanting of HA, cHA and ZncHA spheres in the subcutaneous tissue for 1, and 9 weeks (n=5). The negative control consisted in incision without material implantation (Sham group). The samples were histological processed to descriptive analysis of biological effect. The microscopic analysis showed a similar granulation reaction between groups at the first experimental period. In 9 weeks there was a time dependent biosorption of cHA compared with other groups. In conclusion, the biomaterials tested were biocompatible and cHA group showed a significant biosorption in comparison with HA and ZncHA groups. The doping of zinc did not influence the biocompatibility of biomaterial, however, change the biosorption response

Whole organ sheep kidney tissue engineering and in vivo transplantation: Effects of perfusion-based decellularization on vascular integrity

2019 ◽  
Vol 98 ◽  
pp. 392-400 ◽  
Author(s):  
Abdol-Mohammad Kajbafzadeh ◽  
Reza Khorramirouz ◽  
Behnam Nabavizadeh ◽  
Seyedeh-Sanam Ladi Seyedian ◽  
Aram Akbarzadeh ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Kidney Tissue ◽  
Vascular Integrity

Decellularized bone extracellular matrix in skeletal tissue engineering

2020 ◽  
Vol 48 (3) ◽  
pp. 755-764
Author(s):  
Benjamin B. Rothrauff ◽  
Rocky S. Tuan
Keyword(s):  
Tissue Engineering ◽  
Bone Regeneration ◽  
Tissue Regeneration ◽  
Animal Studies ◽  
Tumor Resection ◽  
Regenerative Capacity ◽  
Skeletal Tissue ◽  
Large Bone

Bone possesses an intrinsic regenerative capacity, which can be compromised by aging, disease, trauma, and iatrogenesis (e.g. tumor resection, pharmacological). At present, autografts and allografts are the principal biological treatments available to replace large bone segments, but both entail several limitations that reduce wider use and consistent success. The use of decellularized extracellular matrices (ECM), often derived from xenogeneic sources, has been shown to favorably influence the immune response to injury and promote site-appropriate tissue regeneration. Decellularized bone ECM (dbECM), utilized in several forms — whole organ, particles, hydrogels — has shown promise in both in vitro and in vivo animal studies to promote osteogenic differentiation of stem/progenitor cells and enhance bone regeneration. However, dbECM has yet to be investigated in clinical studies, which are needed to determine the relative efficacy of this emerging biomaterial as compared with established treatments. This mini-review highlights the recent exploration of dbECM as a biomaterial for skeletal tissue engineering and considers modifications on its future use to more consistently promote bone regeneration.

In vitro- / in vivo- Untersuchungen zur Biokompatibilität und Bioresorption amorpher Kieselgelfaser für das Tissue engineering humaner Knorpelgewebe

2004 ◽  
Vol 83 (02) ◽  
Author(s):  
A Haisch ◽  
A Evers ◽  
K Jöhrens-Leder ◽  
S Jovanovic ◽  
B Sedlmaier ◽  
...  
Keyword(s):  
Tissue Engineering

Surface Modification by Nanobiomaterials for Vascular Tissue Engineering Applications

2020 ◽  
Vol 27 (10) ◽  
pp. 1634-1646 ◽  
Author(s):  
Huey-Shan Hung ◽  
Shan-hui Hsu
Keyword(s):  
Tissue Engineering ◽  
Vascular Tissue ◽  
Thrombus Formation ◽  
Vascular Grafts ◽  
Vascular Tissue Engineering ◽  
Great Success ◽  
Natural Polymers ◽  
Vascular Biomaterials

Treatment of cardiovascular disease has achieved great success using artificial implants, particularly synthetic-polymer made grafts. However, thrombus formation and restenosis are the current clinical problems need to be conquered. New biomaterials, modifying the surface of synthetic vascular grafts, have been created to improve long-term patency for the better hemocompatibility. The vascular biomaterials can be fabricated from synthetic or natural polymers for vascular tissue engineering. Stem cells can be seeded by different techniques into tissue-engineered vascular grafts in vitro and implanted in vivo to repair the vascular tissues. To overcome the thrombogenesis and promote the endothelialization effect, vascular biomaterials employing nanotopography are more bio-mimic to the native tissue made and have been engineered by various approaches such as prepared as a simple surface coating on the vascular biomaterials. It has now become an important and interesting field to find novel approaches to better endothelization of vascular biomaterials. In this article, we focus to review the techniques with better potential improving endothelization and summarize for vascular biomaterial application. This review article will enable the development of biomaterials with a high degree of originality, innovative research on novel techniques for surface fabrication for vascular biomaterials application.

scholarly journals Collagen-Based Electrospun Materials for Tissue Engineering: A Systematic Review

2021 ◽  
Vol 8 (3) ◽  
pp. 39
Author(s):  
Britani N. Blackstone ◽  
Summer C. Gallentine ◽  
Heather M. Powell
Keyword(s):  
Systematic Review ◽  
Tissue Engineering ◽  
Collagen Type I ◽  
The Body ◽  
Pool Data ◽  
Type I ◽  
High Concentrations ◽  
Increased Strength

Collagen is a key component of the extracellular matrix (ECM) in organs and tissues throughout the body and is used for many tissue engineering applications. Electrospinning of collagen can produce scaffolds in a wide variety of shapes, fiber diameters and porosities to match that of the native ECM. This systematic review aims to pool data from available manuscripts on electrospun collagen and tissue engineering to provide insight into the connection between source material, solvent, crosslinking method and functional outcomes. D-banding was most often observed in electrospun collagen formed using collagen type I isolated from calfskin, often isolated within the laboratory, with short solution solubilization times. All physical and chemical methods of crosslinking utilized imparted resistance to degradation and increased strength. Cytotoxicity was observed at high concentrations of crosslinking agents and when abbreviated rinsing protocols were utilized. Collagen and collagen-based scaffolds were capable of forming engineered tissues in vitro and in vivo with high similarity to the native structures.

scholarly journals Extrinsically Conductive Nanomaterials for Cardiac Tissue Engineering Applications

Micromachines ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 914
Author(s):  
Arsalan Ul Haq ◽  
Felicia Carotenuto ◽  
Paolo Di Nardo ◽  
Roberto Francini ◽  
Paolo Prosposito ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cardiac Tissue ◽  
Scar Tissue ◽  
Cardiac Tissue Engineering ◽  
Assistive Device ◽  
Long Run ◽  
Fibrotic Scar ◽  
Regeneration Capability ◽  
Conductive Nanomaterials

Myocardial infarction (MI) is the consequence of coronary artery thrombosis resulting in ischemia and necrosis of the myocardium. As a result, billions of contractile cardiomyocytes are lost with poor innate regeneration capability. This degenerated tissue is replaced by collagen-rich fibrotic scar tissue as the usual body response to quickly repair the injury. The non-conductive nature of this tissue results in arrhythmias and asynchronous beating leading to total heart failure in the long run due to ventricular remodelling. Traditional pharmacological and assistive device approaches have failed to meet the utmost need for tissue regeneration to repair MI injuries. Engineered heart tissues (EHTs) seem promising alternatives, but their non-conductive nature could not resolve problems such as arrhythmias and asynchronous beating for long term in-vivo applications. The ability of nanotechnology to mimic the nano-bioarchitecture of the extracellular matrix and the potential of cardiac tissue engineering to engineer heart-like tissues makes it a unique combination to develop conductive constructs. Biomaterials blended with conductive nanomaterials could yield conductive constructs (referred to as extrinsically conductive). These cell-laden conductive constructs can alleviate cardiac functions when implanted in-vivo. A succinct review of the most promising applications of nanomaterials in cardiac tissue engineering to repair MI injuries is presented with a focus on extrinsically conductive nanomaterials.

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