Molecular identification of two morphologically similar Eulecanium species: E. giganteum and E. kuwanai (Hemiptera: Coccidae)

2015 ◽  
Vol 148 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Jun Deng ◽  
Hai-Bin Li ◽  
Xu-Bo Wang ◽  
Fang Yu ◽  
Yan-Zhou Zhang ◽  
...  
Keyword(s):  
Host Plants ◽  
Ornamental Plants ◽  
Fruit Trees ◽  
28S Rdna ◽  
Similar Species ◽  
Cytochrome C Oxidase I ◽  
Match Analysis ◽  
Accurate Identification ◽  
Close Match ◽  
Coi Sequences

AbstractMost species of the genus Eulecanium Cockerell (Hemiptera: Coccidae) are important economic pests for ornamental plants and fruit trees. Two morphologically similar species, Eulecanium giganteum Shinji and E. kuwanai Kanda, are distributed mainly in China and are quite difficult to identify because of the paucity of distinguishing characteristics, which can only be observed in slide-mounted young, adult females. Furthermore, we demonstrate here that the species occur in sympatry and on many of the same host plants. Mitochondrial cytochrome c oxidase I (COI) and the D2–D3 expansion segments of 28S rDNA were used for accurate identification of these two Eulecanium species from 19 different locations in China. The average K2P distances of COI sequences were 0.47% in E. kuwanai and 0.32% in E. giganteum, and the interspecific divergences varied from 7.23% to 8.34%. Neighbour-joining (NJ) trees of COI and 28S rDNA revealed two distinct non-overlapping clusters, respectively. Meanwhile, “best close match” analysis also showed that 100% of individuals were classified successfully using COI and 28 S sequences. Differentiating between E. giganteum and E. kuwanai is challenging when using ecological and morphological traits. In contrast, identification using DNA diagnostics appears to be very effective, especially when slide-mounted specimens are difficult to obtain.

scholarly journals First record of Heteropsylla tenuata from Argentina (Hemiptera: Psyllidae), and additional notes on other psyllid species

2021 ◽  
Vol 80 (04) ◽  
pp. 142-144
Author(s):  
Carla BALIOTTE ◽  
Daniel A. AQUINO ◽  
Juan P. BOUVET ◽  
Gimena DELLAPÉ
Keyword(s):  
Host Plant ◽  
Geographic Distribution ◽  
Natural Enemies ◽  
Host Plants ◽  
Ornamental Plants ◽  
Fruit Trees ◽  
First Record ◽  
Plant Associations ◽  
Relevant Group ◽  
First Time

The Psyllidae family is the most diverse within Psylloidea, with more than 1,300 species worldwide. Some psyllid species are important pests mainly of fruit trees, forest and ornamental plants. Despite being an economically relevant group, there are few studies on its biology, geographic distribution, natural enemies or host plant associations for many Argentinean psyllid species. This work records one species from Argentina for the first time, extends the distribution of another two psyllid species and provides information on host plants.

SPECIES, HOST RANGE, AND IDENTIFICATION KEY OF WHITEFLIES OF BOGOR AND SURROUNDING AREA

2019 ◽  
Vol 18 (2) ◽  
pp. 127
Author(s):  
Purnama Hidayat ◽  
Denny Bintoro ◽  
Lia Nurulalia ◽  
Muhammad Basri
Keyword(s):  
Host Range ◽  
Host Plants ◽  
Ornamental Plants ◽  
Identification Key ◽  
Agricultural Crops ◽  
Surrounding Area ◽  
Aleurodicus Dispersus ◽  
Agricultural Plants ◽  
Surrounding Areas ◽  
Forest Plants

Species identification, host range, and identification key of whiteflies of Bogor and surrounding area. Whitefly (Hemiptera: Aleyrodidae) is a group of insects that are small, white, soft-bodied, and easily found on various agricultural crops. Whitefly is a phytophagous insect; some species are important pests in agricultural crops that can cause direct damage and can become vectors of viral diseases. The last few years the damage caused by whitefly in Indonesia has increased. Unfortunately, information about their species and host plants in Indonesia, including in Bogor, is still limited. Kalshoven, in his book entitled Pest of Crops in Indonesia, published in the 1980s reported that there were 9 species of whitefly in Indonesia. The information on the book should be reconfirmed. Therefore, this study was conducted to determine whitefly species and its host plants in Bogor and its surroundings. Whiteflies is identified based on the ‘puparia’ (the last instar of the nymph) collected from various agricultural plants, ornamental plants, weeds, and forest plants. A total of 35 species of whiteflies were collected from 74 species and 29 families of plants. The collwcted whiteflies consist of four species belong to Subfamily Aleurodicinae and 31 species of Subfamily Aleyrodinae. The most often found whitefly species were Aleurodicus dispersus, A. dugesii, and Bemisia tabaci. A dichotomous identification key of whiteflies was completed based on morphological character of 35 collected species. The number of whitefly species in Bogor and surrounding areas were far exceeded the number of species reported previously by Kalshoven from all regions in Indonesia.

scholarly journals Non-indigenous scale insects on ornamental plants in Bulgaria and China: A survey

2017 ◽  
Vol 19 (2) ◽  
pp. 114 ◽  
Author(s):  
K. Trencheva ◽  
G. Trenchev ◽  
R. Tomov ◽  
S.-A. Wu
Keyword(s):  
Host Plants ◽  
Ornamental Plants ◽  
Scale Insect ◽  
Scale Insects ◽  
Insect Species ◽  
First Report ◽  
The Family

A preliminary list of non-indigenous scale insect species on ornamental plants in Bulgaria and China is presented. The sampling was done between April and November, 2009, in the framework of the project “Invasive scale insects on ornamental plants in Bulgaria and China”. The insects were collected in nurseries, parks, gardens, botanical collections and greenhouses. Representatives from four families have been identified in Bulgaria, the most numerous of which are the Diaspididae (eight species), Coccidae (four species), Pseudococcidae (two species) and Margarodidae (one species). Three species of non-indigenous scale insects associated with ornamental plants were collected in China, all belonging to the family Pseudococcidae. A list of alien scale insect species on ornamental plants is given, including the sampling sites, host plants on which they were found, origin and first report in both countries.

Cydia molesta. [Distribution map].

1967 ◽  
Author(s):  
Keyword(s):  
North America ◽  
South America ◽  
Host Plants ◽  
Pacific Islands ◽  
Fruit Trees ◽  
Oriental Fruit Moth ◽  
Distribution Map ◽  
Cydia Molesta ◽  
New Distribution ◽  
Distribution In Europe

Abstract A new distribution map is provided for Cydia molesta[Grapholita molesta] (Busck) (Lep., Tortricidae) (Oriental Fruit Moth). Host Plants: Peach and many other fruit trees, etc. Information is given on the geographical distribution in EUROPE, France, Germany, Greece, Hungary, Italy, Malta, Switzerland, Yugoslavia, ASIA, China, Hong Kong, Japan, Korea, Taiwan, Turkey, AFRICA, Morocco, AUSTRALASIA and PACIFIC ISLANDS, Australia, NORTH AMERICA, Canada, Mexico, U.S.A., SOUTH AMERICA, Argentina, Brazil, Uruguay.

High-Throughput Sequencing for Life-History Sorting and for Bridging Reference Sequences in Marine Gerromorpha (Insecta: Heteroptera)

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Jia Jin Marc Chang ◽  
Yin Cheong Aden Ip ◽  
Lanna Cheng ◽  
Ismael Kunning ◽  
Ralph R Mana ◽  
...  
Keyword(s):  
Life History ◽  
High Throughput Sequencing ◽  
Association Studies ◽  
Morphological Characters ◽  
Adult Males ◽  
Accurate Identification ◽  
Life History Stages ◽  
Coi Sequences ◽  
Primer Sets ◽  
Insect Taxonomy

Abstract Accurate identification and association of larval specimens with adults is a major challenge in insect taxonomy. Fortunately, it is now possible for nonexperts to sort collections of bulk samples with DNA barcodes rapidly and cost-effectively. We demonstrate this process using nanopore barcoding of 757 marine insects (Insecta: Gerromorpha), of which 81% were nymphs and many samples did not have co-occurring adult males for specific identification. We successfully associated 738 specimens (97%) to nine gerromorphan species, which would have been impossible to identify using morphological characters alone. This improved ability to incorporate information from all life-history stages has led to greater precision of species distributional ranges—knowledge that will be crucial for a more complete understanding of marine insects. We also highlighted two distinct, nonoverlapping Gerromorpha COI sequence databases on GenBank—a consequence of using two different primer sets to amplify different regions of COI. This issue inevitably hinders species identification with DNA-based methods, particularly for poorly represented groups such as marine insects. We bridged these databases by analyzing full-length COI sequences. We believe this will inspire future studies to incorporate DNA-based methods for more adult–larval association studies and for enhancing existing genetic resources, especially in understudied groups.

scholarly journals Synergistic effect of benzolinone with IBA and fungicides on the vegetative propagation of ornamental plants, park, and fruit woody species

2012 ◽  
Vol 29 (No. 2) ◽  
pp. 41-50 ◽  
Author(s):  
M. Henselová
Keyword(s):  
Root System ◽  
Root Formation ◽  
Ornamental Plants ◽  
Woody Species ◽  
Fruit Trees ◽  
Indole Butyric Acid ◽  
Rooted Cuttings ◽  
Actinidia Arguta ◽  
Allochthonous Species

  A study was made of the stimulating effect of benzolinone (3-benzyloxycarbonyl-methyl)-2-benzothiazolinone in powder form (P) and its mixture with indole butyric acid (IBA), the biofungicide Supresivit and fungicide Captan 50 WP on the root formation of 17 species of ornamental plants, park and fruit woody species. Under the action of benzolinone, the yield of rooted cuttings in the category of ornamental plants amounted to 44.5% (in the Dianthus sp.) up to 83.7% (in Rosa canina), in the category of park woody species it was from l6.7 (in Magnolia stellata) to 100% (in Forsythia intermedia). In fruit trees represented by two species, viz. Actinidia arguta and cherry rootstocks, the yield was between 20 and 86.7%, and in two allochthonous species Cotoneaster horizontalis and Philadelphus coronarius it was from 96.7 to 100% in rooted cuttings. In the case of herbaceous and semi-lignified cuttings, lower concentrations of benzolinone below 0.1% were more effective, while on the contrary, higher concentrations of 0.1–0.2% proved more effective with lignified cuttings. In the species with difficult root formation, such as Magnolia stellata, Viburnum farreri and Actinidia arguta, benzolinone demonstrably stimulated both the rhizogenesis, and thereby the overall yield of rooted cuttings, and the quality of the root system. In the case of the species with naturally high rooting ability, the positive effect of benzolinone was manifest mainly in the quality of the root system. The synergistic action of the above stimulants on root formation was achieved in two and three-component benzolinone mixtures with IBA and fungicides on the basis of the active ingredients captan and Trichoderma harzianum. The most evident stimulating effects on the rooting of cuttings in Dianthus sp., Ficus schlechteri and the deciduous species Acer saccharinum Pyramidale, Prunus padus Colorata and Prunus kurilensis Brilliant was obtained in three-component mixtures.

scholarly journals New records of fruit trees as host for Neosilba species (Diptera, Lonchaeidae) in southeast Brazil

2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Laura Jane Gisloti ◽  
Manoel A. Uchoa ◽  
Angelo Prado
Keyword(s):  
Species Diversity ◽  
Host Plants ◽  
New Records ◽  
Fruit Trees ◽  
Fruit Production ◽  
Native Plant ◽  
Native Plant Species ◽  
Paulo State ◽  
Host Fruit ◽  
Fruit Species

Abstract Fruits of thirty-five cultivated native plant species (19 orders and 12 families) were sampled in farms of fruit production from two municipalities of São Paulo state, Brazil (January 2010 to March 2012) to evaluate species diversity of Neosilba flies. Thirty-one species of plants were the host for Neosilba species while four were not infested. Some aspects of the biology and patterns of species diversity, abundance, infestation rates, puparias viability and the interactions among species of frugivorous flies and their host plants were quantified. Seven species of Neosilba were reared: Neosilba bella Strikis & Prado (4 hosts), Neosilba certa (Walker) (4 hosts), Neosilba glaberrima (Wiedemann) (5 hosts), Neosilba inesperata Strikis & Prado (6 hosts) Neosilba pendula (Bezzi) (15 hosts), Neosilba pradoi Strikis & Lerena (8 hosts) and Neosilba zadolicha McAlpine (26 hosts). The association between the lance flies and the host fruit species is discussed.

A new Blepharosis species from China (Lepidoptera, Noctuidae)

Zootaxa ◽  
2019 ◽  
Vol 4576 (3) ◽  
pp. 596
Author(s):  
PETER GYULAI ◽  
AIDAS SALDAITIS ◽  
ANDRO TRUUVERK ◽  
GINTAS VAITONIS
Keyword(s):  
Male Genitalia ◽  
Flight Period ◽  
Similar Species ◽  
Undescribed Species ◽  
Western Sichuan ◽  
Coi Sequences ◽  
Lepidoptera Noctuidae

In 2017 the second author encountered a rather small Noctuidae species in western Sichuan (China) with unicolorous brown forewings and ochre reniform stigmata. Initially the six males collected resembled the taxa of the genus Cerapteryx Curtis, 1833, but the more gracile body, the finely serrate (and not bipectinated) male antennae and the late flight period indicated the need of further study. Dissection of the male genitalia revealed that the peculiar species belongs to the genus Blepharosis Boursin, 1964. The most recent review with descriptions of new Blepharosis taxa is available from Hreblay, Ronkay & Plante (1998). Comparison of external and genitalia features of the newly found taxon with the known species confirmed that it represents an undescribed species, which is very different externally from all but one of the members of Blepharosis. Regarding the configuration of the male genitalia, the only similar species is Blepharosis anachoretoides (Alphéraky, 1892), displaying only surprisingly small differences between the two species. The large difference between their barcodes (13.5% difference in the COI sequences) indicate however their specific distinctness despite their similar male genitalia structures. 

Five decades of research on phytoplasma-induced witches' broom diseases.

2021 ◽  
Vol 16 (002) ◽  
Author(s):  
Chamran Hemmati
Keyword(s):  
Plant Species ◽  
Ornamental Plants ◽  
Fruit Trees ◽  
The Other ◽  
Economic Losses ◽  
Common Disease ◽  
Vegetable Crops ◽  
Management Options ◽  
Disease Symptoms ◽  
Broom Disease

Abstract Phytoplasmas, prokaryotic wall-less microorganisms, are important pathogens of several plant species in most parts of the world. Phytoplasmas have been reported associated with various symptoms on hundreds of plant species. Witches' broom disease (WBD) is one of the most common disease symptoms, which is caused by phytoplasma strains belonging to different phytoplasma groups. Symptoms of the disease differ from one host to the other as well as from one phytoplasma strain to the other. However, WBD symptoms are usually characterized by the production of a large number of small leaves, accompanied in some host plants by the production of several branches/shoots. Phytoplasma strains belonging to more than 13 groups and 39 subgroups have been reported associated with WBD in more than 116 plant species. Most of the phytoplasma strains causing WBD symptoms in plant species belong to the 16SrII and 16SrI groups, mainly 16SrII-D and 16SrI-B subgroups. The current review provides information on the different types of phytoplasma strains associated with WBD symptoms in ornamental plants, medicinal plants, forest trees, weeds, vegetable crops, field crops, and fruit trees. Emphasis is on WBD on acid limes, almonds, peanuts, jujube, and cassava that have resulted in significant economic losses in different countries. Description of the symptoms, phytoplasma groups, and management options is also provided for some of the diseases.

Traditional Mexican Dish Is Associated With More Than One Skipper Species (Lepidoptera, Hesperiidae, Megathiminae, Aegialini)

2019 ◽  
Vol 113 (3) ◽  
pp. 183-192 ◽  
Author(s):  
Ibeth Jaimes-Rodríguez ◽  
Héctor González-Hernández ◽  
Celina Llanderal-Cázares ◽  
Alejandro Rodríguez-Ortega ◽  
Ariel W Guzmán-Franco
Keyword(s):  
Developmental Stages ◽  
Economic Value ◽  
Adult Emergence ◽  
Diet Breadth ◽  
Molecular Techniques ◽  
Similar Species ◽  
Accurate Identification ◽  
Wing Patterns ◽  
Agave Salmiana ◽  
C 50

Abstract The white maguey worm, Aegiale hesperiaris (Walker, 1856), is a gastronomic delicacy in Mexico, with high economic value. Aegiale hesperiaris is generally associated with the plant Agave salmiana Otto ex Salm-Dyck (Asparagales: Asparagaceae). However, lack of information about Ae. hesperiaris means that it is often confused with morphologically similar species such as Agathymus remingtoni D. Stallings & Thurner (Lepidoptera: Hesperiidae), a species generally found on Agave lechuguilla Torrey (Asparagales: Asparagaceae). Harvestings are made from all Agave L. (Asparagales: Asparagaceae) species on the assumption that all larvae will be Ae. hesperiaris. This has led to the belief that Ae. hesperiaris may have extended its diet breadth range and is actually infesting other Agave species. We collected larvae from A. lechuguilla and A. salmiana plants, in Hidalgo state, Mexico, and incubated them at 26°C, 50% RH and 12:12 light regime until adult emergence; adults were examined using morphological (male genitalia and wing patterns) and molecular techniques (partial COI sequences) to provide the data necessary for accurate species identification and allocation to host plant. Both species were successfully separated and identified as Ae. herperiaris feeding on A. salmiana and Ag. remingtoni feeding on A. lechuguilla, and a detailed description of the larval developmental stages and feeding behavior described. These results will facilitate the accurate identification of these two species in future studies.

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