scholarly journals EVALUATION OF LISTERIA MONOCYTOGENES IN RAW BUFFALO MILK DURING PRIMARY PRODUCTION

2009 ◽  
Vol 1 (4) ◽  
pp. 49 ◽  
Author(s):  
N. Murru ◽  
M. Barile ◽  
M. Tozzi ◽  
C. Ceres ◽  
G. Aprea ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Primary Production ◽  
Buffalo Milk

scholarly journals Growth kinetics of Salmonella Typhimurium and Listeria monocytogenes in buffalo milk under different processing and storage conditions

2021 ◽  
Vol 51 (11) ◽  
Author(s):  
Joelson Sousa Lima ◽  
Ana Paula Presley Oliveira Sampaio ◽  
Mylla Christy da Silva Dufossé ◽  
Paula Fernanda Morais de Sousa ◽  
Josyane Brasil da Silva ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Salmonella Typhimurium ◽  
Growth Kinetics ◽  
Storage Conditions ◽  
Buffalo Milk ◽  
Production Chain ◽  
Pasteurized Milk ◽  
Processing And Storage ◽  
And Storage ◽  
Kinetics Of

ABSTRACT: Buffalo milk is rich in nutrients and can serve as a substrate for the proliferation of microorganisms. Thus, the objective of the present study was to evaluate the growth kinetics of Salmonella Typhimurium and Listeria monocytogenes in buffalo milk under different processing and storage conditions. Samples of raw and pasteurized milk were inoculated with 1 CFU of each bacterium, separately and together, per 25 mL. After contamination, samples were stored at 8 °C or 37 °C, and bacterial counts were performed at 24, 48, and 168 h. In addition, the accompanying microbiota growth, pH, and the effect of these variables on the growth kinetics of microorganisms were monitored. The pathogens tested were able to proliferate under most conditions tested, reaching high titers throughout the experimental period. At 37 °C, there was a decrease in pH and an increase in the accompanying microbiota that interfered with the microbial growth curve. It was also observed that pasteurized milk subjected to 8 °C provided better conditions for the multiplication of bacteria. Therefore, it was concluded that care throughout the production chain, storage, and commercialization of milk must be adopted to guarantee the microbiological safety of this food.

scholarly journals Standardization of a rapid quadruplex PCR method for the simultaneous detection of bovine, buffalo, Salmonella spp., and Listeria monocytogenes DNA in milk

2021 ◽  
Vol 73 (4) ◽  
pp. 781-790
Author(s):  
J.S. Lima ◽  
A.P.P.O. Sampaio ◽  
M.C.S. Dufossé ◽  
A.M.B.P. Rosa ◽  
P.F.M. Sousa ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Dna Sequences ◽  
Performance Optimization ◽  
Genetic Material ◽  
Simultaneous Detection ◽  
Buffalo Milk ◽  
Salmonella Spp ◽  
Initial Inoculum ◽  
Target Dna ◽  
Pcr Method

ABSTRACT The objective of the present study was to Standardize a Polymerase Chain Reaction (PCR) protocol for the authentication of bovine and buffalo milk, and to detect the presence of Salmonella spp. and Listeria monocytogenes. For this, the target DNA was extracted, mixed, and subjected to a PCR assay. Milk samples were defrauded and experimentally contaminated with microorganisms to assess the detection of target DNA at different times of cultivation, bacterial titers, and concentration of genetic material. In addition, the protocol was tested with DNA extracted directly from food, without a pre-enrichment step. The proposed quadruplex PCR showed good accuracy in identifying target DNA sequences. It was possible to simultaneously identify all DNA sequences at the time of inoculation (0h), when the samples were contaminated with 2 CFU/250mL and with 6h of culture when the initial inoculum was 1 CFU/250mL. It was also possible to directly detect DNA sequences from the food when it was inoculated with 3 CFU/mL bacteria. Thus, the proposed methodology showed satisfactory performance, optimization of the analysis time, and a potential for the detection of microorganisms at low titers, which can be used for the detection of fraud and contamination.

scholarly journals Listeria monocytogenes dissemination in farming and primary production: Sources, shedding and control measures

Food Control ◽  
2021 ◽  
Vol 120 ◽  
pp. 107540
Author(s):  
C. Rodriguez ◽  
B. Taminiau ◽  
E. García-Fuentes ◽  
G. Daube ◽  
N. Korsak
Keyword(s):  
Listeria Monocytogenes ◽  
Primary Production ◽  
Control Measures ◽  
And Control

Serotyping and antibiotic resistance of Listeria monocytogenes isolated from raw water buffalo milk and milk products

2020 ◽  
Vol 85 (9) ◽  
pp. 2889-2895
Author(s):  
Goknur Terzi Gulel ◽  
Ali Gucukoglu ◽  
Ozgur Cadirci ◽  
Erdem Saka ◽  
Mustafa Alisarli
Keyword(s):  
Antibiotic Resistance ◽  
Listeria Monocytogenes ◽  
Water Buffalo ◽  
Buffalo Milk ◽  
Raw Water ◽  
Milk Products

scholarly journals Study of the population dynamics of Listeria monocytogenes and Pseudomonas fluorescens in buffalo-milk mozzarella cheese by means of challenge testing

2016 ◽  
Vol 5 (3) ◽  
Author(s):  
Donatella Nava ◽  
Salvatore Capo ◽  
Vincenzo Caligiuri ◽  
Valerio Giaccone ◽  
Loredana Biondi ◽  
...  
Keyword(s):  
At Risk ◽  
Population Dynamics ◽  
Listeria Monocytogenes ◽  
Pseudomonas Fluorescens ◽  
Bacterial Load ◽  
Challenge Test ◽  
Buffalo Milk ◽  
Mozzarella Cheese ◽  
Challenge Testing

Campania buffalo mozzarella is a greatly appreciated cheese in Italy and worldwide. From a microbiological standpoint, it is a highly perishable food and potentially at risk of contamination by pathogens such as <em>Listeria monocytogenes</em> (<em>L. monocytogenes</em>). The present paper reports the results of a challenge test carried out with the aim to assess the population dynamics of <em>L. monocytogenes</em>, alone and in the presence of <em>Pseudomonas fluorescens</em> (<em>Ps. fluorescens</em>), in buffalo mozzarella. For this purpose buffalo mozzarella samples were contaminated with <em>L. monocytogenes</em> alone or combined with <em>Ps. fluorescens</em>. In samples wherein <em>L. monocytogenes</em> was inoculated alone, the bacterial load remained unchanged. By contrast, in samples contaminated with <em>L. monocytogenes</em> and <em>Ps. fluorescens</em>, the growth of <em>L. monocytogenes</em> was increased.

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