scholarly journals Comparative analysis of the internal and external outlines of wings for an outline-based geometric morphometric approach to distinguish three Aedes mosquitoes (Diptera: Culicidae) in Thailand

Author(s):  
T. Chaiphongpachara ◽  
S. Laojun

Aedes mosquitoes belong to a subgenus of Stegomyia (Diptera: Culicidae), which are dengue and chigunkunya vectors, including Aedes aegypti, Ae. albopictus, and Ae. scutellaris, and have a worldwide distribution in tropical and subtropical regions. Species identification of mosquitoes is challenging yet necessary to select appropriate control methods for each species. In this study, we have analyzed the different wing components for identification to find the optimal outline for Aedes mosquito analysis by an outline-based GM approach. Two internal and external outlines of the wing were considered for Aedes species identification in this study. A total of 90 Aedes wings were analysed, divided into 30 wings per species (Ae. aegypti, Ae. albopictus, and Ae. scutellaris). The results showed that the mean size of Ae. albopictus had the largest wings, followed by Ae. Aegypti, and Ae. scutellaris, respectively, in all the internal and external outlines. While, the shape analysis in the three outlines were expressed by the factor map, the statistical difference and cross-validated classification scores indicated the ability of the outline-based GM approach to distinguish species of Aedes mosquitoes. The external outline has a relatively good range of cross-validated classification scores compared to the other internal outlines. The results of this research reveal the suitability of this external outline to identify species of Aedes vectors in Thailand.

2021 ◽  
Vol 29 (2) ◽  
Author(s):  
Latifah Saiful Yazan ◽  
Banulata Gopalsamy ◽  
Siti Najiha Abu Bakar ◽  
Khairul Aiman Manan ◽  
Noranis Shahida Shahidan ◽  
...  

This study reports the efficacy of I.M.O.S (Intelligent Mosquito System) by using Xmos mini aerosol in reducing Aedes mosquito population in 17th College, Universiti Putra Malaysia (UPM). Prior to the experiment, the Aedes mosquito population was determined in all blocks of the 17th College, UPM. The I.M.O.S. was installed above the entrance door of the hall and two rooms and was set to automatically spray at 6.30 am and 4.00 pm every day. No intervention was used in the control house. Adult efficacy study was conducted by placing 20 Aedes mosquitoes in each cage and was hung at a distance of 10 feet from the I.M.O.S. The mortality caused by I.M.O.S throughout the exposed two hours and after 24 hours of exposure was recorded. The number mosquito eggs population were calculated throughout the installation of I.M.O.S. Data were analysed using two-way ANOVA and paired T-test, respectively. The mean number of Aedes mosquito eggs and ovitraps index showed no significant difference (p>0.05) between control and treatment blocks. There were significant differences (p<0.05) in the percentage of knockdown of adult Aedes mosquitoes (10, 20, 30, 60 and 120 minutes after exposure) and the mortality of adult Aedes mosquitoes after 24 hours exposure. Nano and slow-release technology of the I.M.O.S with Xmos mini aerosol proved that this type of intervention can kill adult Aedes mosquitoes. Thus, it is a potential intervention for vector control and management.


2019 ◽  
Vol 20 (2) ◽  
pp. 419-424
Author(s):  
TANAWAT CHAIPHONGPACHARA ◽  
SEDTHAPONG LAOJUN

Chaiphongpachara T, Laojun S. 2019. Short Communication: Landmark-based geometric morphometric analysis of wings to distinguish the sex of Aedes mosquito vectors in Thailand. Biodiversitas 20: 419-424. Aedes mosquitoes (Diptera: Culicidae) are medically important insects which are vectors of yellow fever, dengue fever, chikungunya, West Nile, and the Zika virus, emerging problems worldwide. Typically, male (non-vector) and female (vector) Aedes mosquitoes can easily be separated, however, the samples in the field is often incomplete, making it difficult to separate male and female mosquitoes. The goal of this research is to study the effectiveness of the landmark-based geometric morphometric technique to distinguish the sex of male and female Aedes mosquito vectors, including Ae. aegypti, Ae. albopictus, and Ae. scutellaris, in Thailand. Evaluation of wing size by centroid size analysis found that males and females are distinctly different; females are larger than males in three species of Aedes mosquito. The wing centroid size of Ae. aegypti and Ae. albopictus were very similar, however, Ae. scutellaris was smaller than in both other species. The wing shape between sexes was different in all groups of Aedes mosquitoes. The accuracy of the sex’s classification of Aedes vectors was quite high (more than > 80% from the cross-validated reclassification test). The results of this study prove that landmark-based geometric morphometric can distinguish sexes in Aedes vectors which can be used to solve problems in the field when it is necessary to distinguish the sexes of Aedes mosquitoes with damaged samples.


1994 ◽  
Vol 59 (6) ◽  
pp. 1301-1304
Author(s):  
Jaroslav Nývlt ◽  
Stanislav Žáček

Lead iodide was precipitated by a procedure in which an aqueous solution of potassium iodide at a concentration of 0.03, 0.10 or 0.20 mol l-1 was stirred while an aqueous solution of lead nitrate at one-half concentration was added at a constant rate. The mean size of the PbI2 crystals was determined by evaluating the particle size distribution, which was measured sedimentometrically. The dependence of the mean crystal size on the duration of the experiment exhibited a minimum for any of the concentrations applied. The reason for this is discussed.


2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Ali Pouryousef ◽  
Erfan Eslami ◽  
Sepehr Shahriarirad ◽  
Sina Zoghi ◽  
Mehdi Emami ◽  
...  

Abstract Objectives The current study aimed to evaluate the effects of Ficus carica latex on the treatment of cutaneous leishmaniasis (CL), induced by Leishmania major. A 5% topical gel with F. carica latex was prepared. BALB/c mice were infected by inoculation of amastigotes form of L. major. Thirty BALB/c mice were divided into five groups, where the first group was treated daily, the second group twice per day, and the third group every other day with the 5% topical gel, for 3 weeks. The sizes of the lesions were measured before and during the course of treatment. Results Although the mean size of lesions in the mice group treated with the 5% F. carica gel, especially in the group receiving daily treatment, was less than the mean size of the lesions in the control group, yet, the differences was not statistically significant (p > 0.05). The findings of the current study demonstrated that the 5% F. carica latex with a 3-week course of treatment had no considerable effect in recovery or control of CL induced by L. major in the murine model. Using higher concentration of F. carica latex and with longer treatment lengths may increase its efficacy in the treatment of CL.


Minerals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 653
Author(s):  
Shereef Bankole ◽  
Dorrik Stow ◽  
Zeinab Smillie ◽  
Jim Buckman ◽  
Helen Lever

Distinguishing among deep-water sedimentary facies has been a difficult task. This is possibly due to the process continuum in deep water, in which sediments occur in complex associations. The lack of definite sedimentological features among the different facies between hemipelagites and contourites presented a great challenge. In this study, we present detailed mudrock characteristics of the three main deep-water facies based on sedimentological characteristics, laser diffraction granulometry, high-resolution, large area scanning electron microscopy (SEM), and the synchrotron X-ray diffraction technique. Our results show that the deep-water microstructure is mainly process controlled, and that the controlling factor on their grain size is much more complex than previously envisaged. Retarding current velocity, as well as the lower carrying capacity of the current, has an impact on the mean size and sorting for the contourite and turbidite facies, whereas hemipelagite grain size is impacted by the natural heterogeneity of the system caused by bioturbation. Based on the microfabric analysis, there is a disparate pattern observed among the sedimentary facies; turbidites are generally bedding parallel due to strong currents resulting in shear flow, contourites are random to semi-random as they are impacted by a weak current, while hemipelagites are random to oblique since they are impacted by bioturbation.


2020 ◽  
pp. 112067212097604
Author(s):  
Reem R Al Huthail ◽  
Yasser H Al-Faky

Objective: To evaluate the effect of chronicity on the size of the ostium after external dacryocystorhinostomy (DCR) with intubation. Methods: Design: A retrospective chart review of patients who underwent external DCR with intubation over 10 years from January 2003 at a tertiary hospital. All patients were recruited and examined with rigid nasal endoscope. Results: A total number of 66 (85 eyes) patients were included. The mean age at the time of evaluation was 53.1 years with gender distribution of 54 females (81.8 %). The mean duration ±SD between the date of surgery and the date of evaluation was 33.2 ± 33.6 (6–118 months). Our study showed an overall anatomical and functional success of 98.8% and 95.3%, respectively. The mean size of the ostium (±SD) was 23.0 (±15.7) mm2 (ranging from 1 to 80.4 mm2). The size of the ostium was not a significant factor for failure ( p = 0.907). No statistically significant correlation was found between the long-term duration after surgery and the size of the ostium ( R: 0.025, p = 0.157). Conclusions: Nasal endoscopy after DCR is valuable in evaluating the ostium with no observed potential correlation between the long-term follow-up after surgery and the size of the ostium.


1989 ◽  
Vol 157 ◽  
Author(s):  
E. Johnson ◽  
L. Gråbaek ◽  
J. Bohr ◽  
A. Johansen ◽  
L. Sarholt-Kristensen ◽  
...  

ABSTRACTIon implantation at room temperature of lead into aluminium leads to spontaneous phase separation and formation of lead precipitates growing topotactically with the matrix. Unlike the highly pressurised (∼ 1–5 GPa) solid inclusions formed after noble gas implantations, the pressure in the lead precipitates is found to be less than 0.12 GPa.Recently we have observed the intriguing result that the lead inclusions in aluminium exhibit both superheating and supercooling [1]. In this paper we review and elaborate on these results. Small implantation-induced lead precipitates embedded in an aluminium matrix were studied by X-ray diffraction. The (111) Bragg peak originating from the lead crystals was followed during several temperature cycles, from room temperature to 678 K. The melting temperature for bulk lead is 601 K. In the first heating cycle we found a superheating of the lead precipitates of 67 K before melting occurred. During subsequent cooling a supercooling of 21 K below the solidification point of bulk lead was observed. In the subsequent heating cycles this hysteresis at the melting transition was reproducible. The full width of the hysteresis loop slowly decreased to 62 K, while the mean size of the inclusions gradually increased from 14.5 nm to 27 nm. The phenomena of superheating and supercooling are thus most pronounced for the small crystallites. The persistence of the hysteresis loop over successive heating cycles demonstrate that its cause is intrinsic in nature, and it is believed that the superheating originates from the lack of free surfaces of the lead inclusions.


Author(s):  
Jeanne-Marie Krischer ◽  
Karolin Albert ◽  
Alexander Pfaffenroth ◽  
Elena Lopez-Rodriguez ◽  
Clemens Ruppert ◽  
...  

AbstractMechanical ventilation triggers the manifestation of lung injury and pre-injured lungs are more susceptible. Ventilation-induced abnormalities of alveolar surfactant are involved in injury progression. The effects of mechanical ventilation on the surfactant system might be different in healthy compared to pre-injured lungs. In the present study, we investigated the effects of different positive end-expiratory pressure (PEEP) ventilations on the structure of the blood–gas barrier, the ultrastructure of alveolar epithelial type II (AE2) cells and the intracellular surfactant pool (= lamellar bodies, LB). Rats were randomized into bleomycin-pre-injured or healthy control groups. One day later, rats were either not ventilated, or ventilated with PEEP = 1 or 5 cmH2O and a tidal volume of 10 ml/kg bodyweight for 3 h. Left lungs were subjected to design-based stereology, right lungs to measurements of surfactant proteins (SP−) B and C expression. In pre-injured lungs without ventilation, the expression of SP-C was reduced by bleomycin; while, there were fewer and larger LB compared to healthy lungs. PEEP = 1 cmH2O ventilation of bleomycin-injured lungs was linked with the thickest blood–gas barrier due to increased septal interstitial volumes. In healthy lungs, increasing PEEP levels reduced mean AE2 cell size and volume of LB per AE2 cell; while in pre-injured lungs, volumes of AE2 cells and LB per cell remained stable across PEEPs. Instead, in pre-injured lungs, increasing PEEP levels increased the number and decreased the mean size of LB. In conclusion, mechanical ventilation-induced alterations in LB ultrastructure differ between healthy and pre-injured lungs. PEEP = 1 cmH2O but not PEEP = 5 cmH2O ventilation aggravated septal interstitial abnormalities after bleomycin challenge.


Solar Physics ◽  
1989 ◽  
Vol 122 (2) ◽  
pp. 209-213 ◽  
Author(s):  
C. Macris ◽  
Th. Prokakis ◽  
D. Dialetis ◽  
R. Muller
Keyword(s):  

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