Chronic exposure to high fat diet exacerbates arsenic-induced lung damages in male mice: Possible role for oxidative stress

Arsenic is a common environmental and occupational contaminant worldwide which can influence the development of respiratory diseases. In recent years, alteration in the lifestyle as well as food habits have led to increased consumption of food containing high levels of fat. The present study was designed to evaluate the effects of chronic exposure to a high-fat diet (HFD) on arsenic-induced damages and oxidative stress in the lung tissue of mice. This is the first study to reveal the effect of diet-induced obesity on arsenic-induced lung damages. Seventy-two male Naval Medical Research Institute (NMRI) mice were divided into six groups and fed an HFD or standard diet (SD) while being exposed to 25 or 50 ppm of arsenic through drinking water for 20 weeks. At the end of the experiment, the lung weight to body weight ratio; oxidative stress markers, nitrite level, and hydroxyproline content in the lung tissue; and lung histology were evaluated. The results demonstrated that arsenic exposure leads to a significant decrease in the glutathione level and catalase enzyme activity, and significantly increased reactive oxygen species, malondialdehyde, and nitrite level, but it did not affect the superoxide dismutase activity and hydroxyproline content in the lung tissue. Consequently, all the parameters studied aggravated when HFD was consumed along with arsenic. These findings were confirmed by histological examination. Our study showed that HFD increased arsenic-induced lung damages through oxidative stress in mice. These findings could be important for clinical research to protect against arsenic-induced respiratory toxicity in humans.

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Grape seed and skin extract alleviates high-fat diet-induced renal lipotoxicity and prevents copper depletion in rat

Applied Physiology Nutrition and Metabolism ◽

10.1139/apnm-2012-0416 ◽

2013 ◽

Vol 38 (3) ◽

pp. 259-267 ◽

Cited By ~ 12

Author(s):

Kamel Charradi ◽

Salem Elkahoui ◽

Ines Karkouch ◽

Ferid Limam ◽

Ghaith Hamdaoui ◽

...

Keyword(s):

Oxidative Stress ◽

High Fat Diet ◽

Public Health Problem ◽

Grape Seed ◽

Standard Diet ◽

Skin Extract ◽

High Fat ◽

Oxidative Stress Status ◽

Almost All ◽

Copper Depletion

Obesity is a public health problem that contributes to morbidity and mortality from diabetes, heart disease, stroke, and cancers. The purpose of this investigation was to analyse the link between obesity-induced oxidative stress, renal steatosis, and kidney dysfunction, as well as the protective effect of grape seed and skin extract. Rats were fed a standard diet or a high-fat diet for 6 weeks and were either treated or not treated with grape seed and skin extract. Fat-induced oxidative stress was evaluated in the kidney with a special emphasis on transition metals. High-fat diet induced triglyceride deposition and disturbances in kidney function parameters, which are linked to an oxidative stress status and depletion of copper from the kidney. Grape seed and skin extract abrogated almost all fat-induced kidney disturbances. Grape seed and skin extract exerted potential protection against fat-induced kidney lipotoxicity and should find potential application in other kidney-related diseases.

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Renoprotective Effects of PLGA Nanoparticles Loaded with Vitamin E in Wistar Rats, on Hypercaloric Diet

Revista de Chimie ◽

10.37358/rc.20.6.8187 ◽

2020 ◽

Vol 71 (6) ◽

pp. 230-237

Author(s):

Adriana Dinu ◽

Bogdana Virgolici ◽

Daniela Miricescu ◽

Alexandra Totan ◽

Elvira Gagniuc ◽

...

Keyword(s):

Oxidative Stress ◽

Vitamin E ◽

High Fat Diet ◽

Wistar Rats ◽

Male Rats ◽

Antioxidant Vitamin ◽

Standard Diet ◽

Oxidative Stress Markers ◽

High Fat ◽

Stress Markers

Obesity is associated with renal lipomatosis, inflammation and oxidative stress. Vitamin E is a liposoluble antioxidant vitamin and PLGA (polylactic-CO-glycolic acid) nanoparticles (NPs) represent a delivery carrier of this vitamin to the abdominal viscera. The aim of this study is to evaluate the renal and systemic effects of PLGA- NPs loaded with vitamin E in high fat diet Wistar rats. Young heathy Wistar male rats (3months old, n=20) were fed for 4 weeks either with standard diet or with high caloric/fat diet. These two experimental groups were divided in two subgroups and for the following 21 days, one group received PLGA-NPs loaded with vitamin E (1 mg/kg) body and the other didn�t. Metabolic serum parameters, oxidative stress markers ( malondialdehyde, MDA and total thiols) from kidney homogenates were measured by spectrophotometric methods. Hematoxylin-eosin stain histopathologic examination of kidneys was performed. The obesogenic diet increased the serum levels for triglycerides (p[0.001) and renal function markers: urea (p[0.02), creatinine (p[0.02) and cystatin (p[0.01). The treatment reduced the kidney inflammation, prevented tubulonephrosis damage and improved the renal oxidative stress markers (p[0.05) in the high fat diet group. In conclusion, PLGA-NPs loaded with vitamin E had renoprotective effect in high fat diet Wistar rats.

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Effects of sesame oil on learning and memory impairment and biomarkers of oxidative stress in rats with a long-term high-fat diet consumption

10.21203/rs.3.rs-418705/v1 ◽

2021 ◽

Author(s):

Mojtaba Rustaei ◽

Reihaneh Sadeghian ◽

Iraj Salehi ◽

Abdolrahman Sarihi ◽

Siamak Shahidi ◽

...

Keyword(s):

Oxidative Stress ◽

High Fat Diet ◽

Memory Impairment ◽

Antioxidant Properties ◽

Sesame Oil ◽

Male Rats ◽

Control Group ◽

Standard Diet ◽

High Fat ◽

Positive Effects

Abstract Nowadays, high-fat foods are eaten in most societies, which causes memory impairment and anxiety through the oxidative stress pathway. Sesame oil (SO) has potential antioxidant properties. Therefore, the effects of sesame oil on memory impairment and anxiety caused by a high-fat diet (HFD) in male rats were investigated. Eighty male Wistar rats were divided into eight groups (n = 10): control (standard diet; SD), the HFD, SD + SO (0.5, 1, or 2 ml/kg; once/day, gavage), and HFD + SO (0.5, 1, or 2 ml/kg; once/day, gavage) groups. All diets were given to the animals for three months. Finally, behavioral and oxidative stress parameters were measured. The step-through latency of retention test in SD + SO (0.5 or 1 ml/kg) groups increased more than the control group. Also, the Barnes test on training days revealed that the latency time to find the target hole increased in the HFD group compared with the control group. Moreover, the time spent on the open arms in the SD + SO (0.5 ml/kg) group improved remarkably than the control group. Total oxidant (TOS) level in the HFD + SO (0.5, 1, and 2 ml/kg) groups was lower than the HFD group. The level of total antioxidant capacity (TAC) in the SD + SO (2 ml/kg) group was higher than the SD + SO (0.5 ml/kg) group and the amount of thiol in the HFD group decreased compared with the control group. These findings suggest that the positive effects of SO on memory and anxiety are probably due to its antioxidant properties and the elimination of free radicals.

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Effects of Chia (Salvia hispanica L.) Consumption on Oxidative Stress and Inflammation in Adult Female Wistar Rats (P06-090-19)

Current Developments in Nutrition ◽

10.1093/cdn/nzz031.p06-090-19 ◽

2019 ◽

Vol 3 (Supplement_1) ◽

Author(s):

Barbara Pereira da Silva ◽

Marcella Duarte Villas Mishima ◽

Renata Celi Lopes Toledo ◽

Maria Eliza de Castro Moreira ◽

Neuza Costa ◽

...

Keyword(s):

Oxidative Stress ◽

Mrna Expression ◽

Adult Female ◽

High Fat Diet ◽

Wistar Rats ◽

Standard Diet ◽

High Fat ◽

Protein Levels ◽

Tnf Α ◽

And Oxidative Stress

Abstract Objectives The objective is to investigate the influence of chia consumption on inflammation, oxidative stress, and blood lipid profile in adult female ovariectomized Wistar rats fed high-fat diet Methods Forty ovariectomized and 40 intact (SHAM) rats were allocated into 8 groups (n = 10), and received one of the following four diets: standard diet (ST); standard diet + chia (STC); high-fat diet (HF); high-fat diet + chia (HFC) during 126 days. Biochemical parameters and biomarkers of lipid peroxidation, inflammation, and oxidative stress were evaluated. The mRNA expression of proteins involved in inflammation such as PPAR-α, NFκB, TNF-α and Zn-SOD1 were analyzed, as well as TNF-α and IL-1β levels. The results were subjected to ANOVA at 5% probability. Post hoc Duncan test was carried out to compare means among the groups. Test-t was used to compare the same diet group with ovariectomy or not. Results Chia intake increased HDL-c and reduced LDL-c levels. Among the ovariectomized groups, the animals fed chia showed higher SOD mRNA expression and activity. The consumption of the HF did not alter the total antioxidant capacity. In addition, chia consumption did not improve this parameter. Nevertheless, plasma catalase concentration was higher in STC group. MDA concentration was higher in all groups fed HF. PPAR-α mRNA expression was higher in the STC group. Besides, high fat diet consumption was able to reduce the PPAR-α mRNA expression. The NFκB mRNA expression were lower in STC groups. However, mRNA expression and the protein levels of TNF-α were lower in the rats fed standard diet. Nevertheless, the protein levels of IL-1β were lower in the rats fed standard diet and high fat diet with chia. Conclusions In general, ovariectomy did not influence inflammatory and oxidative stress parameters. Chia intake during 126 days was able to improve antioxidant activity, increasing SOD expression, PPAR-α expression, catalase concentration, and HDL-c levels in adults female rats submitted or not to ovariectomy and fed a standard or a high-fat diet. In addition, chia consumption decrease inflammatory markes, IL-1β and LDL-c concentration. Funding Sources Foundation for Research Support of Minas Gerais (FAPEMIG, Brazil); Coordination for the Improvement of Higher Education Personnel (CAPES, Brazil), and the National Counsel of Technological and Scientific Development (CNPq, Brazil). Supporting Tables, Images and/or Graphs

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Swimming training combined with chitosan supplementation reduces the development of obesity and oxidative stress in high fat diet fed mice

British Journal Of Nutrition ◽

10.1017/s0007114521001379 ◽

2021 ◽

pp. 1-31

Author(s):

Zahra Zalaqi ◽

Farshad Ghazalian ◽

Mohammad Javad Khodayar ◽

Atefeh Raesi Vanani ◽

Layasadat Khorsandi ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Profile ◽

High Fat Diet ◽

Dietary Intervention ◽

The Body ◽

Control Group ◽

Standard Diet ◽

High Fat ◽

Swimming Training ◽

Fat Diets

Abstract Obesity is often introduced as one of the metabolic disorders caused by imbalance between energy consumption and metabolisable energy intake. Experts in the field considered obesity as one of the robust risk factors for the life-style associated diseases. The present research examined interventional effects of marine chitosan (CS), swimming training (ST) and combination of CS and ST (CS+ST) in the mice fed with high fat diets (HFD). In this study, sample size was considered more than 3 in groups. Forty mice were randomly divided into 5 groups (n = 8 per group) including control group (received the standard diet), HFD group (received high fat food with 20% fat), HFD+CS group (treated with high fat food with 5% CS), HFD+ST group (treated with high fat diet and ST) and HFD+CS+ST group (treated with high fat food with 5% CS & ST). After 8 weeks the blood glucose, oxidative stress (OS), and lipid profile were measured. Results showed more efficiency of CS+ST in the control of body weight with the increased concentration of HDL-C, OS inhibition via enhancing the body antioxidant capacity in comparison to the ST or CS alone in obese mice. Moreover, lipid profile was improved in CS + ST-treated mice compared with HFD-treated mice, and OS inhibition correlated to the greater activities of the antioxidant enzyme, enhance the lipid oxidation, cholesterol and fatty acid homeostasis. The results suggested that a dietary intervention with a combined ST and CS can be a feasible supplementary for human prevention of obesity.

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Pengaruh Pemberian Ekstrak Anggur Laut terhadap Penurunan Kadar Kolesterol LDL Rattus norvegicus Jantan yang Mendapat Diet Tinggi Lemak

Hang Tuah Medical journal ◽

10.30649/htmj.v17i2.340 ◽

2020 ◽

Vol 17 (2) ◽

pp. 192

Author(s):

RONALDO LAU ◽

SULISTIANA PRABOWO ◽

RIAMI RIAMI

Keyword(s):

Cholesterol Level ◽

Rattus Norvegicus ◽

High Fat Diet ◽

Ldl Cholesterol ◽

White Male ◽

Standard Diet ◽

High Fat ◽

Caulerpa Racemosa ◽

Significant Difference ◽

Male Wistar Rats

ABSTRACTBackground: High fat diet increase the absorption of lipid in the intestinum, that can lead to increase LDL cholesterol level in the blood. Sea grapes extract (Caulerpa racemosa) contains antioxidant polyphenolic group that can reduce MTP and ACAT-2 in the body that can decrease LDL cholesterol level in the blood.The purpose of this study is to know the effect of sea grapes extract on decreasing LDL cholesterol of white male Wistar rats (Rattus norvegicus) fed with high fat diet.Method: 24 white male Wistar rats, that divided into 3 groups: 1) group of rats fed with standard diet for 28 days; 2) group of rats fed with high fat diet for 28 days; 3) group of rats fed with high fat diet for 28 days and given 10 gram/kg body weight/day of sea grapes extract on 15th-28th days. Then the blood LDL cholesterol level measured on the 29th day.Result : One-Way ANOVA Test showed there was significant difference (p=0.004) of LDL level between the group of rats fed with standard diet (12.37 mg/dl) compared to group of rats fed with high fat diet (17.87 mg/dl). There was significant difference (p=0.001) of LDL level between the group of rats fed with high fat diet (17.87 mg/dl) compared to group of rats fed with high fat diet and sea grapes extract (10.12 mg/dl).Conclusion: high fat diet significantly increase blood LDL cholesterol level and sea grapes extract (Caulerpa racemosa) significantly decrease blood LDL cholesterol level. Keywords :Sea grapes extract, LDL cholesterol, high fat diet

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