scholarly journals The spread and antimicrobial resistance of Staphylococcus aureus in South African dairy herds – A review

2021 ◽  
Vol 88 (1) ◽  
Author(s):  
Joanne Karzis ◽  
Inge-Marie Petzer ◽  
Vinny Naidoo ◽  
Edward F. Donkin
Keyword(s):  
South Africa ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Relevant Literature ◽  
Treatment Strategies ◽  
Economic Loss ◽  
Climatic Conditions ◽  
Effective Manner ◽  
Health Concept

Staphylococcus aureus is internationally recognised as a principal agent of mastitis and the foremost reason for economic loss in the dairy industry. The limited data available on organism-specific antibiotic resistance surveillance in dairy cattle have stimulated the need for such a review article. The objective of this study was to review relevant literature on antimicrobial resistance of mastitis-causing staphylococci isolated from dairy cows in South Africa compared to other countries. Factors relating to the incidence of mastitis and treatment strategies in terms of the One Health concept and food security were included. The Web of Science (all databases) and relevant websites were used, and articles not written in English were excluded. The incidence of mastitis varied between South Africa and other countries. Antimicrobial resistance patterns caused by S. aureus also varied in regions within Southern Africa and those of other countries although some similarities were shown. Antimicrobial resistance differed between S. aureus bacteria that were maltose positive and negative (an emerging pathogen). The results highlighted the importance of the availability of organism-specific surveillance data of the incidence of mastitis and antibiotic resistance for specific countries and within similar climatic conditions. Accurate knowledge about whether a specific pathogen is resistant to an antibiotic within a certain climate, country, area or farm should reduce the incidence of unnecessary or incorrect treatment with antibiotics. This should enable dairy farmers to deal with these organisms in a more effective manner. Therefore such research should be ongoing.

scholarly journals Trends in antibiotic resistance patterns of methicillin resistant and methicillin sensitive Staphylococcus aureus in Khwaja Yunus Ali Medical College Hospital

KYAMC Journal ◽  
2018 ◽  
Vol 9 (1) ◽  
pp. 6-10 ◽  
Author(s):  
Abdullah Akhtar Ahmed ◽  
Md Abdus Salam ◽  
Md Shakhaowat Hossain ◽  
Md Babul Aktar ◽  
Nusrat Akhtar Juyee ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Pathogenic Bacteria ◽  
Current Status ◽  
Clinical Samples ◽  
Sensitivity Testing ◽  
Methicillin Resistant ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns

Background: Antimicrobial resistance in pathogens is a growing concern for health. It is a great concern around the globe about the threat of increasing antimicrobial resistance. In response to this concerns, medical experts have proposed initiatives to curtail the spread of antimicrobial resistance in pathogenic bacteria. We have taken this surveillance study in KYAMCH to contain the spread of antimicrobial resistance.Objectives: To determine the prevalence of MRSA from different clinical samples and to record its current status to commonly used anti Staphylococcus antibiotics.Methods: The clinical samples were taken to the laboratory and within four hours cultured for isolates and identification pathogens. Finally, antibiotic sensitivity testing of the isolated Staphylococcus aureus was performed. Results: More than 80% of MRSA isolates were resistant to ampicillin (98%) followed by amoxicillin, cefixime and azithromycin with 94%, 93.5% and 85% respectively and maximum sensitivity toward tobramycin with 86% followed by 85% gentamicin and 79% meropenem.Conclusion: The detected trend in antibiotic resistance patterns of methicillin-resistant and methicillin-sensitive Staphylococcus aureus in this study is alarming. This has created a huge clinical burden in the hospital settings as well as in the community.KYAMC Journal Vol. 9, No.-1, April 2018, Page 6-10

scholarly journals A retrospective analysis of pathogen profile, antimicrobial resistance and mortality in neonatal hospital-acquired bloodstream infections from 2009–2018 at Tygerberg Hospital, South Africa

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0245089 ◽  
Author(s):  
Kessendri Reddy ◽  
Adrie Bekker ◽  
Andrew C. Whitelaw ◽  
Tonya M. Esterhuizen ◽  
Angela Dramowski
Keyword(s):  
South Africa ◽  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Methicillin Resistance ◽  
Neonatal Unit ◽  
Attributable Mortality ◽  
Published Data ◽  
Period 2 ◽  
Empiric Antibiotic ◽  
Hospital Acquired

Background Analysis of hospital-acquired bloodstream infection (HA-BSI) trends is important to monitor emerging antimicrobial resistance (AMR) threats and guide empiric antibiotic choices. Methods A retrospective 10-year review of neonatal HA-BSI was performed at Tygerberg Hospital’s neonatal unit in Cape Town, South Africa. Neonatal clinical and laboratory data from 2014 to 2018 (Period 2) was compared with published data from 2009 to 2013 (Period 1). Results The neonatal unit’s HA-BSI rate declined between periods from 3.9/1000 inpatient-days in Period 1 to 3.3/1000 inpatient-days in Period 2 (p = 0.002). Pathogen yield and blood culture contamination rate were unchanged (11.0% to 10.4%, p = 0.233; 5.1% to 5.3%, p = 0.636 respectively). Gram-negative pathogens predominated (1047/1636; 64.0%); Klebsiella species, Staphylococcus aureus, Serratia marcescens, Enterococcus species and Acinetobacter baumannii were the most frequent pathogens. Extended spectrum beta-lactamase production was observed in 319/432 (73.8%) of Klebsiella species, methicillin resistance in 171/246 (69.5%) of Staphylococcus aureus and extensive drug resistance in 115/137 (83.9%) of Acinetobacter species (2009–2018). The crude mortality rate of neonatal HA-BSI episodes increased from Period 1 to Period 2 from 139/717 (19.4%) to 179/718 (24.9%) (p = 0.014), but HA-BSI attributable mortality remained unchanged (97/139 [69.8%] vs 118/179 [65.9%], p = 0.542). The in-vitro activity of piperacillin-tazobactam and amikacin declined during Period 2 (74.6% to 61.4%; p<0.001). Conclusion Although HA-BSI rates declined in the neonatal unit, antimicrobial resistance rates in BSI pathogens remained high. Continuous BSI surveillance is a valuable tool to detect changes in pathogen and AMR profiles and inform empiric antibiotic recommendations for neonatal units in resource-limited settings.

scholarly journals Proactive udder health management in South Africa and monitoring of antibiotic resistance of Staphylococcus aureus in dairy herds from 2001 to 2010

2018 ◽  
Vol 89 ◽  
Author(s):  
Joanne Karzis ◽  
Inge-Marie Petzer ◽  
Edward F. Donkin ◽  
Vinny Naidoo
Keyword(s):  
South Africa ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
South African ◽  
Health Management ◽  
Bovine Milk ◽  
Management Programme ◽  
Dairy Herds ◽  
Udder Health ◽  
Cell Counts

Antibiotic resistance of strains of Staphylococcus aureus isolated from bovine milk is of concern internationally. The objective of this study was to investigate trends of resistance of S. aureus to antibiotics administered to dairy cows in 19 South African and one Zambian dairy herds (participating in the South African proactive udder health management programme) and to identify possible contributing factors. The resistance of S. aureus strains to eight commonly used antibiotics in South Africa from 2001 to 2010 was evaluated. Staphylococcus aureus isolates (n = 2532) were selected from cows with subclinical mastitis in 20 herds routinely sampled as part of the proactive udder health management programme. The isolates were selected from milk samples that had somatic cell counts more than 400 000 cells/mL and were tested for antibiotic resistance using a standard Kirby–Bauer test with published clinical breakpoints. The prevalence of antibiotic resistance was evaluated as a percentage of S. aureus isolates susceptible out of the total numbers for each antibiotic selected per year. Staphylococcus aureus showed a significant increase in percentage of susceptible isolates over time for all antibiotics tested except for ampicillin. The overall prevalence of mastitis did not change during the study period. However, the prevalence of mastitis caused by S. aureus (mostly subclinical cases) in the selected herds decreased numerically but not significantly. Reduction in the incidence of antibiotic resistance shown by S. aureus was presumed to be a result of the application of the proactive udder health management programme. The fact that the overall prevalence of mastitis was kept stable was possibly because of the influence of the management programme in conjunction with the return of infections caused by non-resistant strains.

scholarly journals Surveillance of antimicrobial resistance of maltose negative Staphylococcus aureus in South African dairy herds

2020 ◽  
Author(s):  
Joanne Karzis ◽  
Inge-Marié Petzer ◽  
Edward F. Donkin ◽  
Vinny Naidoo ◽  
Eric M. C. Etter
Keyword(s):  
South Africa ◽  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Dairy Herds ◽  
Disk Diffusion Method ◽  
Milk Samples ◽  
Kwazulu Natal ◽  
Over Time

Abstract Background The discovery of antimicrobials in the 1930s was one of the greatest achievements in medicine. However, bacterial resistance to antimicrobials was already observed in the 1940s and has been reported since then in both human and veterinary medicine, including in dairy cows. Many years of monitoring milk samples in South Africa, has led to the identification of a new strain of Staphylococcus aureus (S. aureus), which is maltose negative and appears to be an emerging pathogen. In this study the differences in susceptibility to antimicrobials of this strain were evaluated over time, over different seasons, in different provinces, and according to somatic cell count (SCC) categories. Results A data set of 271 maltose negative S. aureus isolates, cultured from milk samples from 117 herds out of the estimated 2000 commercial dairy herds in South Africa between 2010 and 2017, was studied using the disk diffusion method. This analysis was done using the Clinical Laboratory Standards Institute (CLSI) breakpoints in order to compare using both the previous (Intermediate category grouped with Resistant) and current definitions, (Intermediate category grouped with Susceptible). The results of the analysis between the previous and the current definitions differed for tylosin, cefalonium, oxy-tetracycline and cloxacillin. Neither the analysis using the previous nor the current systems showed an effect of province for the maltose negative S. aureus. This was in contrast to the results for maltose positive S. aureus where differences between provinces were shown in a previous study, with the lowest prevalence of resistance shown in KwaZulu-Natal during spring. For the susceptibility testing of 57 maltose negative and 57 maltose positive S. aureus isolates from 38 farms, from KwaZulu Natal, Eastern Cape and Western Cape. The minimum inhibitory concentration (MIC) results for the maltose negative S. aureus isolates confirmed the results of the disk diffusion method. Conclusions The maltose negative strains of S. aureus differed in general, in their antimicrobial resistance patterns over time, in comparison to maltose-positive S. aureus strains. MIC testing also indicated that more multidrug -resistant isolates were seen with the maltose negative S. aureus than in the maltose positive strains.

scholarly journals Surveillance of antimicrobial resistance of maltose negative Staphylococcus aureus in South African dairy herds

2019 ◽  
Author(s):  
Joanne Karzis ◽  
Inge-Marié Petzer ◽  
Edward F. Donkin ◽  
Vinny Naidoo ◽  
Eric M. C. Etter
Keyword(s):  
South Africa ◽  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Diffusion Method ◽  
Dairy Herds ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Data Set ◽  
Milk Samples ◽  
Over Time

Abstract BackgroundThe discovery of antimicrobials in the 1930s was one of the greatest achievements in medicine. However, bacterial resistance to antimicrobials was already observed in the 1940s and has been reported since then in both human and veterinary medicine, including in dairy cows. Many years of monitoring milk samples in South Africa, has led to the identification of a new strain of Staphylococcus aureus (S. aureus), which is maltose negative and appears to be an emerging pathogen. In this study the differences in susceptibility to antimicrobials of this strain were evaluated over time, over different seasons, in different provinces, and according to somatic cell count (SCC) categories.Results A data set of 271 maltose negative S. aureus isolates, cultured from milk samples from 117 herds out of the estimated 2000 commercial dairy herds in South Africa between 2010 and 2017, was studied using the disc diffusion method. This analysis was done using the Clinical Laboratory Standards Institute (CLSI) breakpoints in order to compare using both the previously used system (intermediate category grouped with resistant) and more recent system, (intermediate category grouped with susceptible). The results between the previously used system and the more recent system analysis differed for tylosin, cefalonium, oxy-tetracycline and cloxacillin. Neither the analysis using the previous system nor the more recent system showed an effect of province for the maltose negative S. aureus. This was in contrast to the results for maltose positive S. aureus where differences between provinces were shown in a previous study. For the susceptibility testing of 57 maltose negative S. aureus and 57 maltose positive S. aureus the minimum inhibitory concentration (MIC) results for the maltose negative S. aureus confirmed the results of the disc diffusion method. ConclusionsThe maltose negative strains of S. aureus differed in general, in their antimicrobial resistance patterns over time, in comparison to maltose-positive S. aureus strains. MIC testing also indicated that more multi-resistant isolates were seen with the maltose negative S. aureus than in the maltose positive strains.

scholarly journals Prevalence of Antibiotic Resistance in Salmonella Serotypes Concurrently Isolated from the Environment, Animals, and Humans in South Africa: A Systematic Review and Meta-Analysis

Antibiotics ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1435
Author(s):  
Tsepo Ramatla ◽  
Mpho Tawana ◽  
ThankGod E. Onyiche ◽  
Kgaugelo E. Lekota ◽  
Oriel Thekisoe
Keyword(s):  
South Africa ◽  
Systematic Review ◽  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Environmental Samples ◽  
Meta Analysis ◽  
Western Cape ◽  
Eastern Cape ◽  
North West ◽  
Salmonella Serotypes

One of the main global concerns is the usage and spread of antibiotic resistant Salmonella serovars. The animals, humans, and environmental components interact and contribute to the rapid emergence and spread of antimicrobial resistance, directly or indirectly. Therefore, this study aimed to determine antibiotic resistance (AR) profiles of Salmonella serotypes isolated from the environment, animals, and humans in South Africa by a systematic review and meta-analysis. The preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines were followed to search four databases for studies published from 1980 to 2021, that reported the antibiotic resistance profiles of Salmonella serotypes isolated in South Africa. The AR was screened from 2930 Salmonella serotypes which were isolated from 6842 samples. The Western Cape province had high pooled prevalence estimates (PPE) of Salmonella isolates with AR profiles followed by North West, Gauteng, and Eastern Cape with 94.3%, 75.4%, 59.4%, and 46.2%, respectively. The high PPE and heterogeneity were observed from environmental samples [69.6 (95% CI: 41.7−88.3), Q = 303.643, I2 = 98.353, Q-P = 0.045], animals [41.9 (95% CI: 18.5–69.5), Q = 637.355, I2 = 98.745, Q-P = 0.577], as well as animals/environment [95.9 (95% CI: 5.4−100), Q = 55.253, I2 = 96.380, Q-P = 0.300]. The majority of the salmonella isolates were resistant to sulphonamides (92.0%), enrofloxacin and erythromycin (89.3%), oxytetracycline (77.4%), imipenem (72.6%), tetracycline (67.4%), as well as trimethoprim (52.2%), among the environment, animals, and humans. The level of multidrug-resistance recorded for Salmonella isolates was 28.5% in this review. This study has highlighted the occurrence of AR by Salmonella isolates from animals, humans, and environmental samples in South Africa and this calls for a consolidated “One Health” approach for antimicrobial resistance epidemiological research, as well as the formulation of necessary intervention measures to prevent further spread.

scholarly journals Bacteriophage Therapy for the Prevention and Treatment of Fracture-Related Infection Caused by Staphylococcus aureus: a Preclinical Study

2021 ◽  
Author(s):  
Jolien Onsea ◽  
Virginia Post ◽  
Tim Buchholz ◽  
Hella Schwegler ◽  
Stephan Zeiter ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Therapeutic Potential ◽  
Case Reports ◽  
Treatment Strategies ◽  
Preclinical Study ◽  
Bacteriophage Therapy ◽  
The Past ◽  
Prevention And Treatment

Because of the growing spread of antimicrobial resistance, the use of alternative prevention and treatment strategies is gaining interest. Although the therapeutic potential of bacteriophages has been demonstrated in a number of case reports and series over the past decade, many unanswered questions remain regarding the optimal application protocol.

Molecular Characterization of Antibiotic Resistance Gene Pattern of Staphylococcus aureus and Escherichia coli in Mastitis Affected Dairy Cows

2020 ◽  
Author(s):  
T. Ramasamy ◽  
S. Keerthana ◽  
M.R. Srinivasan ◽  
D. Chandrasekar ◽  
K. Porteen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Molecular Characterization ◽  
Antibiotic Sensitivity ◽  
Growth Media ◽  
E Coli ◽  
Sensitivity Pattern

Background: Antimicrobial resistance is one of the latest challenges facing the scientific community. Raising the drug resistance is caused mainly by indiscriminate usage of antibiotics in human and animal subjects and the spread of antibiotic resistance between the two has an emerging global threat. Hence, current study aimed to study the antimicrobial resistance pattern and molecular detection of antibiotic resistance genes in Staphylococcus aureus and Escherichia coli isolated from mastitis affected cows. Methods: Milk samples from mastitis affected cows were subjected to antibiotic sensitivity test and screened for presence of Staphylococcus aureus and Escherichia coli using differential growth media. Molecular characterization of Staphylococcus aureus and Escherichia coli was done with the help of PCR by amplification of ‘nuc’ and ‘uspA’ gene respectively. MICs of Penicillin and Tetracycline were determined using microdilution method. Result: Antibiotic sensitivity pattern for Penicillin G, Ampicillin, Amoxycillin, Cefotaxime, Ceftriaxone, Azithromycin, Ciprofloxacin, Gentamicin, Oxytetracycline, Tetracycline and Vancomycin were 74.19%, 100%, 93.50%, 61.29%, 29%, 35.48%, 9.70%, 9.70%, 70.96% and 70.96% respectively. More than 87.90% of the S. aureus and 50% of the E. coli isolated were resistant to â-lactam antibiotics while 75% of the E.coli and 65.70% of the S. aureus isolated were resistant to Tetracycline antibiotics. The MICs of Penicillin for S.aureus and E.coli are 26.88 µg/ml and 13.54 µg/ml respectively and the MICs of Tetracycline for S. aureus and E. coli are 243.75 µg/ml and 960.93 µg/ml respectively which is 8-9 folds higher than the standard MICs. From the present study, it can be inferred that bovine mastitis cases are highly resistant to antimicrobial drugs. Results further indicate that Staphylococcus aureus and Escherichia coli are both resistant to Penicillin and Tetracycline with very high MIC.

Antimicrobial resistance of nosocomial strains Staphylococcus aureus isolates from surgical patients in Kyiv regional clinical hospital, ukraine

2018 ◽  
Vol 2 (1) ◽  
pp. 49-63
Author(s):  
A.G. Salmanov ◽  
V.V. Potochilovа ◽  
K.L. Rudneva
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Antimicrobial Susceptibility ◽  
Surgical Patients ◽  
Local Data ◽  
Clinical Hospital ◽  
Epidemiologic Surveillance ◽  
Microbiological Laboratory ◽  
Amoxicillin Clavulanic Acid

Objective — to determine antimicrobial resistance of nosocomial strains Staphylococcus aureus isolates from surgery patients. Materials and methods. The clinical strains of S. aureus (574) isolated from surgical patients from January 2015 to December 2017 in Kyiv Regional Clinical Hospital of the Ukraine have been studied. Clinical isolates were allocated and identified in Central microbiological laboratory in Kyiv Regional Clinical Hospital. The identification and antimicrobial susceptibility of the cultures were determined, using automated microbiology analyzer VITEK 2 Compact (bioMerieux, France). Susceptibility to antibiotics was determined using AST card (bioMerieux, France). Some antimicrobial susceptibility test used K-B (Kirby – Bauer antibiotic testing). Sensitivity of S. aureus strains has been studied up to 51 antibiotics: penicillum, ampicillin, amoxicillin, azlocillin, ampicillin/sulbactam, amoxicillin/clavulanic acid, oxacillin, cefoperazone/sulbactam, piperacillin/tazobactam, piperacillin, cefazolin, cefuroxime, cefamandolum, cefotaximum, cefoxitin, ceftriaxone, cefixime, cefpodoxime, ceftazidime, cefepim, imipenem, meropenem, ertapenem, kanamycin, gentamicin, tobramyinum, netilmicin, amikacinum, eritromicin, azithromycin, clindamycin, tetracyclin, doxycyclinum, vancomycin, teicoplaninum, ofloxacinum, levofloxacin, ciprofloxacin, moxifloxacin, gatifloxacin, norfloxacin, chloramphenicol, nitrofurantoinum, rifampicin, linezolidum, tigecycline, fosfomicin, acidum fusidicum, mupirocinum, trimethoprimum, and trimethoprim/sulfamethoxazole. Interpretative criteria were those suggested by the European Committee on Antimicrobial Testing (EUCAST). Results and discussion. To S.aureus the drug sensitive rates of piperacillin/tazobactam and ertapenem were both 100 %, but most of the drug resistant rates were over 65—70 %. The highest activity to S. aureus had nitrofurantoinum, cefoxitin, linezolid, trimethoprimum, tigecycline, and trimethoprim/sulfamethoxazole. The high rates of resistance were registered for cefpodoxime (91.7 %), ampicillin (85.0 %), ceftazidime (80.0 %), penicillin (75.0 %), cefixime (73.0 %), cefoperazone/sulbactam (71.5 %), and to azithromycin (69.5 %). The vancomycin — resistant S. aureus (VRSA) accounted for 21.1 % [95 % CI 19.4—22.8 %]. The frequency of isolation of MRSA among tested strains was 37.8 % [95 % CI 35.2—39.2 %] varying from 28.7 % to 44.8 % in different surgical departments in Kyiv Regional Clinical Hospital. Conclusions. Antibiotic resistance to S. aureus in surgical hospitals, being a subject of the research is considered to be a serious therapeutic and epidemiologic problem. Taking into account the constant changes and significant differences of the S. aureus resistance levels observed in various regions, the constant monitoring of antibiotic resistance to antimicrobials in every in-patient medical institution is required and on the base of the local obtained results to elaborate the hospital record sheets. Antibiotics application tactics should be determined in accordance with the local data of resistance to them in each surgical in-patient institution. The system of epidemiologic surveillance over microbial resistance should be established on the local, regional, and national levels.

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