Determining the equivalent phosphorus released by an Escherichia coli-derived phytase in broiler chicks

2004 ◽  
Vol 84 (3) ◽  
pp. 437-444 ◽  
Author(s):  
S. A. Adedokun ◽  
J. S. Sands ◽  
O. Adeola

Seven-day-old male Ross 308 broiler chicks were used in a 14-d experiment to determine the equivalency value of a new phytase using corn-soybean meal based diets. The experimental diets consisted of positive control (PC) or negative control (NC) diets which were formulated to contain 5.0 or 1.2 g kg-1 non-phytate P, respectively, with a total P of 7.7 or 3.9 g kg-1, respectively; and respective calcium levels of 10.0 or 7.6 g kg-1. The test diets were formulated by the addition of monosodium phosphate (MSP; analyzed to contain 224.0 g kg-1 P) to the NC diet to supply 0.5, 1.0, or 1.5 g of P kg-1 diet, and phytase was added to the NC diet at 500 FTU or 1000 FTU kg-1 at the expense of corn starch. Each diet was randomly assigned to eight cages of four birds each. Body weight gain (BWG) showed both linear and quadratic increase (P < 0.05) with increasing level of dietary P or phytase supplementation (450 to 656 or 603 g, respectively). Feed efficiency (g BWG/g feed intake) increased linearly (P < 0.05) with increase in P or phytase supplementation and quadratic effect to increasing level of phytase (P < 0.05) supplementation (0.699 to 0.793 or 0.758 g, respectively). Percent tibia and toe ash increased linearly (P < 0.05) in response to supplemental P from MSP or phytase supplementation (37.7 and 8 to 47.9 and 10.9 or 45.2 and 10, respectively). Total intestinal tract P and N digestibility increased linearly (P < 0.05) with increasing P supplementation and showed increased (linear and quadratic, P < 0.05) response to phytase. The response variables, BWG, toe and tibia ash, feed intake and final body weight were used for P equivalency calculation. By solving the equated linear regression equations for the supplemental inorganic P intake and supplemental phytase intake for each of the response variables, between 0.930 and 1.101 g of P was released by 1000 FTU of phytase. The results of this study showed that this phytase was efficacious in hydrolyzing phytate P for bone mineralization and growth of broiler chicks. Key words: Chicks, equivalency value, monosodium phosphate, phosphorus, phytase

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 94
Author(s):  
Mohammed M. Qaid ◽  
Saud I. Al-Mufarrej ◽  
Mahmoud M. Azzam ◽  
Maged A. Al-Garadi ◽  
Hani H. Albaadani ◽  
...  

Ross 308 broiler chicks (n = 240) aged 1 day were assigned to five groups for eight replicates (six chicks for each) (3♂ and 3♀). Basal dietary groups were supplemented by 2000, 4000, and 6000 mg/kg cinnamon (CN) for 21 days. Basal diet alone was used as a negative control, and basal antibiotic diet (Colimox) was used as a positive control. At 10, 14, and 21 days of age, chicks that received 2000 mg CN and Colimox had a higher body weight, resulting in an increase in body weight gain. CN also resulted in the maximum improvement in the feed conversion ratio and feed efficiency over 1–21 days at the level of 2000 mg/kg. At days 10, the maximum relative breast weight was 2000 mg/kg of CN. Mean serum albumin concentrations, duodenal villus height, and goblet cell density increased (p < 0.05) by 2000 mg/kg of CN, and mean serum globulin and total protein concentrations and crypt depth increased (p < 0.05) by 6000 mg/kg of CN compared with control. Increased cecal Escherichia coli number was CN dose-dependent. In conclusion, dietary inclusion of 2000 mg/kg CN can be applied as an alternative to in-feed antibiotics for broiler starter diet.


2021 ◽  
Vol 17 (1) ◽  
pp. 27-34
Author(s):  
A.I. Ukanwoko ◽  
C.T. Alfred

An experiment was carried out to determine the growth performance and haematological indices of Red Sokoto goats placed on oil palm leaf meal (OPLM)-wheat offal based diet. Six goats aged 12-18 months, weighing 15 – 20kg were randomly assigned to three treatments, comprising two animals each in a Completely Randomized Design. Each animal received 1kg of the diet daily for 56 days and weighed weekly. Results showed that final body weight was significant (P<0.05) with goats on T3 having the highest (21.50kg). The feed intake, body weight gain and Feed Conversion Ratio (FCR) were significantly different (P<0.05). T3 had the highest feed intake (305.35g/d), highest body weight gain (90.84g/d) and the best FCR (3.36). Significant differences (P <0.05) were observed in the Red Blood Cell (RBC), Packed Cell Volume (PCV) and Haemoglobin (Hb) counts, ranging between 3.12 - 4.20x106ml; 23.00 -30.50% and 7.70 - 10.15g/dl respectively with goats on T3 having the highest.  The inclusion of OPLM enhanced body weight gain, FCR, RBC, PCV, and Hb counts of the goats. Therefore the oil palm leaf meal diets are recommended for better goat production. Key word: Growth performance; Oil Palm Leaf meal., Blood profile., Creatinine., Red Sokoto Goat.


2021 ◽  
Vol 41 (1) ◽  
pp. 272-283
Author(s):  
K. T. Orayaga ◽  
P. M. Goholshak

Three groundnut meals namely: groundnut cake (GNC), locally processed groundnut cake (kulikuli) and toasted groundnut seed(kernel) meal (TGSM) were incorporated in rabbit diets at two levels to generate six diets: 18% GNC, 22% GNC, 18% Kulikuli, 22% Kulikuli, 18% TGSM and 22% TGSM respectively, and 18% GNC also served as the control diet. Twenty four (24) six weeks-old growing rabbits of mixed breeds and sexes with mean initial weight of 568.75±l2.85g were randomly allocated to the six dietary treatment groups, such that each had four rabbits and each rabbit formed a replicate in a completely randomized design laid out in a 2X3 factorial arrangement and fed ad libitum, for 70 days. The main effects showed that while the treatment groups fed TGSM were significantly depressed (p<0.05). rabbits fed GNC and kulikuli were not significantly different (p>0.05) for average daily feed intake, daily body weight gain and final body weight. Feed conversion ratio (FCR) was not significantly (p >0.05) affected among treatment groups. Rabbits fed TGSM had significantly (p<0.05) better apparent digestibility coefficient (78.04%) than those on kulikuli (75.81%6) and the group on kulikuli were better than animals fed GNC (72.09%) for fat digestibility. Fibre digestibility was also significantly different (p <0.05), with rabbits fed kulikuli having the poorest fibre digestion (15.63 %). Crude protein, dry matter and NFE apparent digestibility coefficients were not significantly (p >0.05) affected. Among all the parameters measured, only fat and fibre digestibility coefficients had significant (p <0.05) interactions and NFE digestibility was significantly affected (p <0.05) by level of inclusion, with groups fed 22% digesting it better. Feed cost kg gain did not differ significantly (p >0.05) among the treatment groups. Growth was still on the rise and there was a significantly (P<0.01) strong relationship (correlation and regression) between feed intake and weight gain for all the treatment groups. was concluded that both kulikuli and TGSM are potential feed resources for rabbits, more available to house hold farmers, equally economical as GNC and better still if groundnut farmers and or processors of kulikuli raise rabbits, though TGSM had less value for growth rate compared to GNC and Kulikuli at these levels of inclusion


2014 ◽  
Vol 11 (1) ◽  
pp. 1-5 ◽  
Author(s):  
S Khatun ◽  
M Mostofa ◽  
F Alom ◽  
J Uddin ◽  
MN Alam ◽  
...  

The efficacy of tulsi (Ocimum sactum) and neem (Azadirachta indica) leaves extract as a growth promoter were studied in broiler. A total of 40 day-old broiler chicks were purchased from Kazi hatchery and after three days of acclimatization the chicks were randomly divided into four groups (n=10). No vaccination schedule was practiced and no antibiotic was added in ration of group A, B, C, and D respectively. Group A served control without any supplements while group B, C and D were supplemented with combination of tulsi and neem extract @ 1 ml, 2ml and 3 ml/liter of drinking water. Live body weight gain was recorded weekly up to 6th weeks and hematological studies were performed at 21st and 42nd day of experiments. At the end of 42nd day of experiment final body weight of group A, B, C and D were 1561± 12.10 g, 1698± 12.87 g, 1608± 12.04 g and 1763± 13.28 g, respectively. The birds of group D utilized their feed more efficiently among the treatment groups (p<0.05). The net body weight gain were, 1533± 11.98 g, 1443± 11.32 g and 1588± 12.10 g in treated groups compared to control group (1393± 11.07 g ) and total net profit per broiler was 19.08, 40.10, 20.68 and 45.07 Taka , respectively. Hematological parameterss (TEC, PCV, Hb and ESR) were not significantly changed among the treated and control group suggesting no side effects of herbal extracts in broiler. It can be concluded that tulsi and neem extract is economic and safe in broiler production.DOI: http://dx.doi.org/10.3329/bjvm.v11i1.17728 Bangl. J. Vet. Med. (2013). 11 (1): 1- 5


2021 ◽  
Vol 43 (1) ◽  
pp. 254-262
Author(s):  
A. O. Aniebo

The effect of dietary treatments on growth performance, haematological and biochemical indices of finisher broilers as well as economics of broiler production were determined in this fermented cassava flour/blood mixture code-named "cassablood". The control diet (T1) contained 0% cassablood, while diets 2, 3 and 4 contained 20%, 25% and 30% cassablood respectively. 120 four weeks old Anak broiler chicken were randomly allocated the 4 diets with 30 birds per treatment replicated 3 times with ten 10 broilers per replicate in a completely randomized design (CRD). Treatment effects on final body weight, final weight gain, feed intake and seed conversion ratio were significant (P<0.05). Birds fed cassablood based diets compared favourably with the control diet. 20% cassablood inclusion performed equally with the control diet in feed intake, body weight gain and feed conversion ratio.All dietary groups also showed no significant differences (P>0.05) in most haematological and serum biochemical indices of broilers except in cholesterol, glucose and triglyceride contentswhichdiffered (P<0.05) significantly. Similarly, chicken on 20% and 25% inclusion levels produced significantly (P < 0.05) higher economic gains in terms of feed cost per kilogram of broiler among all the treatments. Therefore, 20 - 25% cassablood dietary inclusion level was recommended in broiler diet for production and profit maximization.  


2021 ◽  
Vol 24 (1) ◽  
pp. 26-30
Author(s):  
G. F. Faniyi

Eighty day-old broiler chicken were fed diets in which maize was replaced weight for weight with mango seed-kernel meal at 0, 10, 20, and 30% levels during starter and finisher phases of four weeks each. No significance differences (p>0.05) were observe in the final body weight, body weight gain, feed intake, mortality, feed conversion efficiency and feed efficiency ratio among the chicks. During the starter phase, the highest feed intake value (2001.81 g/birds) was recorded for diet IV and the least value was obtained for diet I (control diet). During  the finisher phase, however, the least feed intake value (773.24 g/bird) was obtained in respect of diet I while feed intake values were similarly high for others diets. It was, therefore, concluded that mango seed-kernel meal could be utilized by broiler chicks during the starter and finisher phase and it could serve as an alternative energy source to maize in broiler starter and finisher diets.


2021 ◽  
Author(s):  
Muhammad Asif ◽  
Zafar Hayat ◽  
Abdur Rahman ◽  
Muhammad Fiaz Qamar ◽  
Muawuz Ijaz ◽  
...  

Abstract The study was designed to investigate the effect of mannan-oligosaccharide (MOS) supplementation on intestinal histomorphology, immunity against Newcastle disease virus (NDV) and productive parameters of broilers. A total of 1800 day old broiler chicks of Cobb-500 strain were selected and randomly assorted into 6 treatment groups: T1 (basal diet without antibiotics as negative control); T2 (basal diet plus antibiotics as positive control group); T3 (basal diet plus 200g/ton MOS); T4 (basal diet plus 400g/ton MOS); T5 (basal diet plus 600g/ton MOS) and T6 (basal diet plus 800g/ton MOS). Each treatment was having 6 replicates and the feed intake, body weight gain and feed conversion ratio (FCR) were recorded on weekly basis. Results showed that, MOS supplemented birds have significantly higher feed intake, weight gain and FCR (P < 0.05). Similarly, supplementation of MOS showed positive effect on villus height and crypt depth both in jejunum and ilium. Goblet cell density was unaffected by MOS addition (P < 0.05). Furthermore, birds fed with diets containing MOS, exhibited better productive performance in comparison to positive and negative control groups. In conclusion, MOS can replace antibiotic growth promoters (AGPs) as non-microbial performance-enhancing feed advocates.


2020 ◽  
Vol 46 (4) ◽  
pp. 153-160
Author(s):  
B. C. Anyaegbu ◽  
D. N. Onunkwo ◽  
G. Igwe ◽  
J. Nathaniel ◽  
C. M. Nkwo

Considering the inherent attributes of tannia cocoyam (Xanthosoma sagitifolium) its appropriate utilization may enhance performance of starter broiler chicks. This study was therefore carried out to determine the dietary evaluation of starter broiler chicks fed cooked cocoyam tuber meal as energy source in place of maize. The cooked cocoyam tuber meal was used to replace maize at various levels of 25%, 50%, 75% and 100% respectively for diets 2, 3, 4 and 5 to determine the best replacement level that would give optimal performance in starter broiler chicks' diets. Result of the proximate composition of cooked cocoyam tuber meal showed that it contained 13.11% moisture, 2.60% ash, 1.85% ether extract, 3.90% crude protein, 9.45% crude fibre, 70.13% nitrogen free extract and 3004.37 Kcal/kgME. 150 day old Anak broiler chicks were used for the experiment. They were divided into 5 treatment groups of 30 broiler chicks each. Each treatment group was subdivided into 3 replicates of 10 broiler chicks each and kept in deep litter. Each starter diet was fed to a group of 30 starter broilers for 4 weeks using completely randomized design. They were given feed and water adlibitum. Parameters measured include initial body weight, final body weight, body weight gain, feed intake, feed conversion ratio and economics of production. In the starter broiler feeding trial, the starter broilers on 25% cooked cocoyam meal compared favourably with the control (P> 0.05) in terms of feed intake, body weight gain and feed conversion ratio and significantly (P > 0.05) performed between than those on 100% CCYM. The cost of production (N/Kg broiler) was however cheapest for diet 2 (25% CCYM) N395 versus control diet (N494.28). It was therefore concluded that cooked cocoyam tuber meal (Xanthosoma sagitifolium) could be used in the diets of starter broiler chicks' up to 25% without affecting the body weight gain, feed intake and feed conversion ratio as indicated in this study.


2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Sanjeev Wasti ◽  
Nirvay Sah ◽  
Amit K. Singh ◽  
Chin N. Lee ◽  
Rajesh Jha ◽  
...  

Abstract Background Heat stress is a significant problem in the poultry industry, causing a severe economic loss due to its detrimental effects on chickens’ health and performance. Dried plum (DP) is a good source of minerals, vitamins, antioxidants, and phenolic compounds. Studies have suggested that DP has several health benefits, such as maintaining the body’s redox system, immune status, and calcium hemostasis. Based on the health benefits of DP, we hypothesized that the dietary supplementation of DP would alleviate the detrimental effects of heat stress on broiler chickens. Results To test the hypothesis, day-old broiler chicks (n = 72) were randomly allocated to three treatment groups (n = 24/group): no heat stress (NHS), heat stress (HS), and heat stress with dried plum (HS + DP), and reared under standard conditions. The inclusion of 2.5% DP in the feed of the HS + DP group was made during the treatment period, while birds in other groups were provided with a standard finisher diet. After 21 days, birds in the HS and HS + DP groups were exposed to cyclic heat stress conditions (33 °C for 8 h during daytime) for 3 weeks, while those in the NHS group were reared under normal conditions (22–24 °C). Weekly body weight and feed intake were recorded to calculate the average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (FCR). Heat stress significantly decreased the final body weight, ADG, ADFI, and increased FCR compared to the NHS group, whereas dietary supplementation of DP significantly improved these growth performance parameters compared to the HS group. Furthermore, supplementation of DP significantly increased the expression of heat shock protein-related genes (HSF1, HSF3, HSP70, and HSP90), antioxidant-related genes (SOD1, SOD2, GPX1, GPX3, PRDX1, and TXN), tight junction-related genes (CLDN1, and OCLN), and immune-related genes (IL4, MUC2) in the ileum as compared to the HS group. The microbiota analysis showed significant enrichment of Bacillales, Christensenellaceae, Bacillaceae, Peptostreptococcaceae, and Anaerotruncus in heat-stressed birds supplemented with DP as compared to the HS group. Further, DP supplementation also significantly increased the concentration of acetate, propionate, and total VFA in the cecal digesta of the HS + DP group as compared to the HS group. Conclusion These findings suggest that DP supplementation effectively improved the growth performances and gut health parameters in the heat-stressed birds. Thus, dried plum can be a potential feed supplement to mitigate heat stress in broiler chickens.


2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Amit K. Singh ◽  
Birendra Mishra ◽  
Michael R. Bedford ◽  
Rajesh Jha

Abstract Background This study evaluated the effects of supplemental xylanase and xylooligosaccharides (XOS) in a corn-soybean meal (SBM)-based diet on growth performance and intestinal health of broilers. A total of 288 day-old chicks (Cobb 500) were allocated to 36 floor pens (8 birds/pen) equally in 9 dietary treatments in a 3 × 3 factorial arrangement. The treatments were combinations of 3 levels of xylanase (0, 0.005% and 0.01% Econase XT) and 3 levels of prebiotics (0, 0.005% and 0.01% XOS) added to basal mash diets formulated in three phases (starter, d 0–14; grower, d 15–28; finisher, d 29–42). The feed intake and body weights were recorded weekly. On d 42, ileal sections were collected for histomorphometric and gene expression analysis, and cecal content was collected for determining short-chain fatty acids (SCFA) and microbiota. Results Xylanase linearly (P < 0.01) increased the average daily gain (ADG) in both the finisher and total period and the final body weight gain (FBWG, 2940 & 2932 vs. 2760 g) of broilers. XOS did not significantly increase either ADG or FBWG (P > 0.05). Supplemental xylanase and XOS did not affect average daily feed intake and feed conversion ratio (P > 0.05). Xylanase and XOS did not change villus height (VH) or crypt depth (CD) ratio (P > 0.05). However, xylanase exhibited a trend (P = 0.097) on VH:CD ratio. The inclusion of 0.01% XOS without xylanase increased the level of IL-10 (a marker of anti-inflammatory cytokine) and IL-4 (a T-cell differentiation cytokine) genes compared with control (P < 0.05). The acetate production was increased by xylanase (P < 0.01) and XOS (P < 0.05) without an additive effect. Xylanase increased total SCFA (P < 0.01) while XOS had a tendency to increase (P = 0.052). Alpha and beta diversity of microbiota among treatments were not different (P > 0.05). However, the mean proportion of family Ruminococcaceae was increased by the supplemental 0.01% xylanase (P < 0.01). Conclusion It can be concluded that XOS can enhance cecal fermentation, while xylanase can increase the body weight gain along with the fermentation metabolites in the ceca of broilers fed the corn-SBM-based diet but the effects may not always translate into an improved mucosal absorptive capacity and a better feed efficiency.


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