Efficacy of new bacterial phytase in poultry diets

2000 ◽  
Vol 80 (3) ◽  
pp. 527-528 ◽  
Author(s):  
S. Leeson ◽  
H. Namkung ◽  
M. Cottrill ◽  
C. W. Forsberg

Phytase produced by Escherichia coli was fed to broiler chicks fed graded levels of available phosphorus (0.25–45%). A positive control involved Natuphos® a fungal phytase commonly used in poultry nutrition. Feeding diets with only 0.25% available phosphorus from 1 to 13 d of age resulted in reduced feed intake and weight gain, with associated loss in tibia weight, tibia ash and tibia phosphorus content (P < 0.01). Adding 600 IU phytase kg−1 diet corrected these problems, and both sources were equally efficacious for most parameters studied. Key words: Phosphorus, phytase, chicken

2014 ◽  
Vol 44 (8) ◽  
pp. 1479-1485 ◽  
Author(s):  
Luciana de Paula Naves ◽  
Paulo Borges Rodrigues ◽  
Levy do Vale Teixeira ◽  
Antônio Gilberto Bertechini ◽  
Renata Ribeiro Alvarenga ◽  
...  

The reduction of the available phosphorus (avP) content in the broiler diet must be followed by the supplementation of adequate phytase. One experiment was conducted with broilers from 15 to 28 days of age to determine the level of necessary phytase when the mashed diet is formulated with 0.213% of avP to enable the results of performance, tibia ash content, and calcium (Ca) utilization similar to those determined for birds fed with diet formulated to meet their nutritional requirements. Thus, 120 broilers were distributed in (4+1) x2 factorial arrangement corresponding to four deficient diets in avP (0.213%) supplemented with phytase (0; 750; 1,500 or 2,250FTU kg-1) plus one positive control diet without phytase (0.426% of avP), supplied to male and female broilers. Males showed better performance and higher totalP retention. Decrease in the avP content of the diet without phytase use worsened the performance, tibia ash content, and Ca retention; however, these parameters were improved gradually with the increase of the phytase level in the diet. Regardless of sex, using 2,250FTU kg-1, it is possible to reduce the avP to 0.213% without impairing performance, tibia ash content, and Ca retention; in addition to reducing the totalP excretion in 56.75% and improving its utilization in 38.58%.


2013 ◽  
Vol 53 (4) ◽  
pp. 316 ◽  
Author(s):  
M. Günal

An experiment was conducted to investigate the effects of sodium gluconate and microbial phytase (MP) (Natuphos 1000G) supplementation of diets on performance, mineral retention and bone mineralisation in male broiler chicks from a day old to 21 days of age. The experiment was carried out using a completely randomised design with a 3 by 2 factorial arrangement (0, 2 and 4% sodium gluconate and 0 and 750 U MP/kg diet). Diets were formulated with deficient contents of available phosphorus (aP) (2.4 g/kg). Ten replicate cages of four chicks per replicate cage were fed experimental diets. MP supplementation of diets with deficient contents of aP significantly improved weight gain (P < 0.05) and feed efficiency (P < 0.01). The performance results were similar in birds fed the diets without sodium gluconate and the diets with 2% sodium gluconate. However, the dietary inclusion of sodium gluconate at 4% depressed (P < 0.05) the growth of broiler chickens compared with the diet without sodium gluconate. Compared with the diets supplemented with 2% sodium gluconate, the diets supplemented with 4% sodium gluconate decreased (P < 0.05) weight gain and increased (P < 0.05) feed efficiency. MP supplementation increased (P < 0.01) Ca, P, Mg, Zn retention, and tibia ash, Ca and P contents in tibia ash. Diets with sodium gluconate increased (P < 0.05) P and Zn retention. Diets containing sodium gluconate without phytase increased (P < 0.05) Mg retention and P content in tibia ash. The diet with 2% sodium gluconate without phytase also increased (P < 0.05) Ca retention. The dietary inclusion of sodium gluconate at 2% improved (P < 0.05) tibia ash and Ca content in tibia ash. Sodium gluconate and MP had significant interactions in Ca and Mg retention (P < 0.01), and P content (P < 0.05) in tibia ash. In conclusion, the results indicated that phytase and sodium gluconate supplementation of corn–soybean meal low AP diets increased Ca, P, Mg and Zn mineral retention and bone mineralisation in chicks. The addition of MP improved the performance. However, the supplementation of diets with 4% sodium gluconate depressed the performance.


2020 ◽  
Vol 8 (2) ◽  
pp. 61-73
Author(s):  
R. Davin ◽  
C. Kwakernaak ◽  
Y. Dersjant-Li

A study evaluated the effects of limestone sources with different solubility on the efficacy of two phytases in broilers. A 2×5 factorial arrangement was employed with two commercial limestone sources and five diet treatments; a positive control (PC) diet with 7.2 g/kg P and 9.6 g/kg Ca, and a negative control diet containing reductions of 1.87 g/kg available P, 1.99 g/kg Ca and 0.4 g/kg Na, supplemented with either Buttiauxella phytase (PhyB) or Escherichia coli phytase (PhyE) at 500 or 1000 FTU/kg diet. The two limestone sources were feed-grade commercial products with different particle sizes and solubility (fast-soluble (FS) 100%; slow-soluble (SS) 26% soluble after 30 minutes at pH 3) containing similar levels of Ca. Diets were fed to one-day-old Ross 308 males (n=2,400) with 30 birds/pen and eight pens/treatment in two phases (starter 0-10 d and grower 10-21 d). On d 21, ileal digesta was collected from 12 birds/pen to determine apparent ileal digestibility (AID) of P and Ca, and myo-inositol hexakisphosphate (IP6) disappearance, and tibias from four birds/pen for ash determination. The SS limestone improved body weight gain (BWG), feed intake (FI) and FCR vs FS limestone in starter/grower phases (P<0.05), and improved AID of P (P<0.05) and IP6 disappearance (P<0.05) at d 21. There was an interaction between limestone and phytase on BWG, FI and FCR in the grower phase (P≤0.05) whereby FS (vs SS) limestone reduced BWG at either dose of PhyE, but only at 500 FTU/kg of PhyB. At an equivalent dose, PhyB had higher BWG and feed intake than PhyE (P<0.05). At 1000 FTU/kg, performance was equivalent (BWG and FI) or superior (FCR) to the PC, PhyB produced greater tibia ash, AID of P and IP6 disappearance (P<0.05). The findings showed that the effects of limestone particle size on phytase efficacy varied with phytase source and dose.


2010 ◽  
Vol 90 (1) ◽  
pp. 57-63 ◽  
Author(s):  
M N Haque ◽  
K M.S. Islam ◽  
M A Akbar ◽  
R Chowdhury ◽  
M Khatun ◽  
...  

The goal of this research was to investigate the effect of citric acid (CA), the antibiotic growth promoter flavomycin (FL) and their combination on the growth performance, tibia ash and immune status of broilers. One hundred and sixty straight run day-old broiler chicks (Hubbard Classic) were randomly distributed into four dietary groups, with four replicate cages having 10 birds in each. Standard corn-soybean-based basal starter diet was given to control birds. Diets for other groups were further fortified by 0.5% CA, 0.001% FL and their combination (0.5% CA plus 0.001% FL). On day 35, CA and its combination (CA + FL) group had 17 and 12% increase in live weight compared with control, respectively (P < 0.05). Total live weight on day 35 was 1141, 1337, 1169, and 1275 g bird-1 in different groups, respectively (P < 0.05). Cumulative feed intake was higher (P < 0.05) in CA and its combination (CA + FL) compared with the control and FL groups on day 28. Supplementation with CA improved (P > 0.05) feed conversion ratio [FCR; feed intake (kg):weight gain (kg)] compared with FL and its combination (CA + FL). Carcass yield was increased (P > 0.05) due to the addition of CA and its combination (CA + FL). Tibia ash content in the CA group (59.0) was higher (P < 0.05) than in the control (53.6%), FL (53.1%) and their combination (57.1%). The lymphocyte cells associated with immunity in the lymphoid organs (caecal tonsil, bursa Fabricius and ileum) were more densely populated, suggesting an increased level of innate immunity in the CA group. Overall mortality was low (1.25%), and there were no treatment-related effects on mortality. Citric acid reduced the pH of formulated feeds, but mild reduction was found in the pH of the faeces. Dietary supplementation of 0.5% CA increase weight gain, feed intake, tibia ash deposition and non-specific immunity (P < 0.05) as well as feed efficiency and carcass yield (P > 0.05) of broilers. Citric acid has potential as a growth promoter to replace the antibiotic growth promoter FL.Key words: Citric acid, flavomycin, growth performance, immunity, tibia ash, broiler


2010 ◽  
Vol 90 (4) ◽  
pp. 529-536 ◽  
Author(s):  
T.A. Woyengo ◽  
A. Emiola ◽  
A. Owusu-Asiedu ◽  
W. Guenter ◽  
P.H. Simmins ◽  
...  

The bio-efficacy of Escherichia coli-derived phytase produced by thermo-protective coating technology (coated phytase) was evaluated in mash or pelleted broiler diets. Three hundred and twenty-four broilers were divided into 54 groups of six birds and fed nine corn-based diets (six replicates per diet) from 1 to 22 d of age. The nine diets were a positive control (PC) (calcium, 0.9% and non-phytate phosphorus, 0.45%) fed as mash plus 2 × 4 factorial of a negative control (calcium, 0.78% and non-phytate phosphorus, 0.26%) fed as mash (NC-M) or pellet (NC-P) and with coated phytase at 0, 500, 600 or 700 FTU kg-1. The diets were pelleted at 80°C, and pressure load of 40 psi. Body weight gain (BWG) for the PC diet was higher (P < 0.001) than that for the NC-M or NC-P diet. The BWG and tibia ash were linearly increased (P < 0.001) by increased level of the phytase in NC-M or NC-P diet. The BWG for NC-M or NC-P diet reached that of the PC diet when phytase was supplemented at 600 or 700 FTU kg-1. In conclusion, the coated phytase improved nutrient utilization in broilers, and its bio-efficacy was unaffected by the pelleting process.


2015 ◽  
Vol 11 (2) ◽  
pp. 111-116
Author(s):  
BA Makanjuola ◽  
OO Obi ◽  
TO Olorungbohunmi ◽  
OA Morakinyo ◽  
BA Boladuro ◽  
...  

An experiment was conducted to determine the influence of an acidifier as an alternative to antibiotics on the performance and gut morphology of broiler chickens. One hundred and eighty (180) 7-day old broiler chicks were randomly assigned to 3 dietary treatments of 4 replicates each. Each replicate had 15 birds. The diets fed to the birds were: Treatment 1- diet with neither antibiotic nor acidifier; Treatment 2- diet with antibiotic and Treatment 3- diet with acidifier. The antibiotic used was Enrofloxacin which was administered to the birds on Treatment 2 via their drinking water while the acidifier used was Biotronic SE (a combination of propionic and formic acids) which was added to the diet of birds on Treatment 3 at the rate of 4kg/tonne of feed. The experiment lasted 42 days. No significant differences were observed in the feed intake, final weight and weight gain of the birds on the different diets. Significant differences were however observed in the villus height in the duodenum and ileum of the birds. The crypt depth values in the different segments of the small intestines of the birds were not significantly affected by the different treatments.Key words: Organic acids, Biotronic SE, Enrofloxacin, Crypt, villus.


Author(s):  
Caroline Bavaresco ◽  
Everton Luis Krabbe ◽  
Diego Surek ◽  
Edenilse Gopinger ◽  
Fernando Nicolas Martinez ◽  
...  

Abstract The objective of this work was to evaluate the effect of the use of hybrid phytase, alone or combined with carbohydrases, in poultry diets with nutritional reductions of calcium, available phosphorus, and metabolizable energy on the nutrient digestibility and bone quality of broiler chickens. A total of 1,875 broilers were distributed in five treatments in a completely randomized design, with 15 replicates of 25 chickens each. The treatments consisted of a positive control feed (T1) and of four negative controls (T2 to T5): T1, basal diet (BD) with corn and soybean; T2 and T3, BDs with reductions of 70 and 100 kcal kg-1 metabolizable energy, respectively, and both with reductions of 0.16% Ca and 0.15% available P; and T4 and T5, BDs with the same nutritional reductions, but supplemented with enzymes, i.e., T4 = T2 + 500 phytase units (FTU) per kilogram and T5 = T3 + 500 FTU kg-1 + 560 xylanase units (TXU) per kilogram + 250 glucanase units (TGU) per kilogram. The use of 500 FTU kg-1 hybrid phytase in pelleted corn-soybean meal diets allows a good digestive performance by broilers and replaces the nitrogen-corrected apparent metabolizable energy at 70 kcal kg-1, as well as 0.16% Ca and 0.15% available P.


2020 ◽  
Vol 47 (3) ◽  
pp. 67-76
Author(s):  
D. N. Onunkwo ◽  
O. A. Ekine

This study was conducted to assess the effects of abattoir wastes (bovine blood and rumen content mixture) on the haematology and growth performance characteristics of broiler birds. One hundred and twenty (120) unsexed day old Abor acre broiler chicks were randomly assigned to 4 treatments and replicated 3 times in a completely randomized design (CRD) experiment. Four experimental diets were formulated to meet the nutrient requirement of broilers such that the birds were fed sundried abattoir waste (bovine blood and rumen content mixture) included at 0%, 5%, 10% and 15% representing Diet 1, 2, 3 and 4 respectively, with Diet 1 as the control. Each dietary treatment consists of 30 birds with 10 birds per replicate. The experimental diets and clean water were supplied at- libitum throughout the experimental period. Data were collected on feed intake, daily weight gain, final body weight, weight gain. Feed conversion ratios were calculated. Blood samples for hematology was collected, analyzed and recorded. At the end of the 56 days feeding trials, data collected on different parameters were subjected to analysis of variance (ANOVA). Results showed that mixtures of bovine blood and rumen content significantly (P<0.05) improved the final weight (1767.02g -1931.44g), weight gain (1367.02g -1528.44g), feed intake (107.47g -118.99g) and feed conversion ratio (4.87 – 4.08) of the broilers. Significant (P<0.05) improvements were observed on the RBC, WBC, MCV, MCH, eosinophyls and the lymphocytes of the haematological parameters of the broilers. These results showed the complementary potentials of bovine blood and rumen content mixture in broiler diets, which can be included up to 15% in the ration of broilers without detrimentally affecting the growth performance and haematology of the broilers.


Author(s):  
M. R. Abdollahi ◽  
F. Zaefarian ◽  
Y. Gu ◽  
W. Xiao ◽  
J. Jia ◽  
...  

SummaryThe influence of different inclusion levels of a biologically active peptide derived from soybeans by enzymatic hydrolysis, on growth performance, foot pad lesions and carcass characteristics in broilers were examined in this study. Starter (1 to 21 d) and finisher (22 to 42 d) diets, based on maize and soybean meal, were subjected to seven inclusion levels of a commercial soybean bioactive peptide (SBP) product (Fortide, Chengdu Mytech Biotech Co. Ltd., Chengdu, Sichuan, China) at 0.0, 1.0, 2.0, 3.0, 4.0, 5.0 and 6.0 g/kg of diet. All diets were equivalent in respect of energy density, digestible amino acids and other nutrients. A total of 840, one-day-old male broilers (Ross 308) were allocated to 42 pens (20 birds/pen), which were randomly assigned to seven dietary treatments. During the starter period, there was no significant effect of SBP on weight gain and feed intake of the birds. However, a significant (P < 0.05) effect of SBP was observed for the feed conversion ratio (FCR), with SBP inclusion at 3.0 g/kg and above showing lower (P < 0.05) FCR values compared to the diet with no SBP. No effect of SBP was observed for weight gain and feed intake over the whole trial period. However, SBP inclusion tended (P = 0.06) to influence the FCR of birds. Increasing SBP inclusion level resulted in gradual decrease in FCR values, with SBP inclusion at 5.0 and 6.0 g/kg showing lower FCR values compared to the diet with no SBP. Overall, the present study suggests that dietary supplementation of SBP in broiler diets has the potential to improve FCR and to be used as a novel functional protein in poultry diets.


2018 ◽  
Vol 47 (2) ◽  
pp. 85-91
Author(s):  
MR Tiwari ◽  
PK Jha ◽  
B Sah ◽  
G Kunwar ◽  
AK Jha

An attempt was taken to study the growth performance of lemongrass oil (Cymbopogon citraturs) as a growth promoter on the broiler production. A total of 180day-old broiler chicks were procured from private hatchery (Shivam Hatchery Birgung, Parsa, Nepal) and were allocated following Completely Randomized Design (CRD) into four treatment groups (each treatment with 3 replications and each replication with 15 birds); T1= control, T2= containing lemongrass oil 200 ml/100 kg feed, T3= containing lemongrass oil 400 ml/100 kg feed and, T4= containing lemongrass oil 600 ml/100 kg feed. The study was carried out for 36 days at Avian Research Unit, Regional Agriculture Research Station, Parwanipur, Bara, Nepal. Concentrate mixture and lemongrass oil were procured from Shakti Feed Industry, Birgung; Herbs Processing Plant of Ministry of Forest and Soil Conservation, Jadibuti, Kathmandu, respectively. Experimental birds were provided ad libitum amount of starter feed from 0 to 21 days and finisher feed from 22 to 36 days. Feed intake was recorded daily and body weight gain was measured in 7 days interval. The study revealed that the cumulative feed intake per bird was found higher in T3 (3443.17g) followed by T4 (3377.33g) and T2 (3354.04g), respectively, and were statistically non-significant among the diet groups. The FCR was highest in T3 (1:1.44 kg) and lowest in T4 (1:1.51 kg), and differed insignificantly among the diet groups. Similarly, the total weight gain of the experimental bird was found to be higher in T3 (2385.13g) followed by T1 (2279.46g) and T2 (2271.86g), respectively.  The average daily gain of experimental birds noted higher in T3 (66.25g) followed by T1 and T2 (63.31g) and (63.1g), respectively. The experiment suggested that inclusion of lemongrass oil could be considered as an alternative to antibiotic growth promoter in broiler diet to enhance the production performance. Bang. J. Anim. Sci. 2018. 47 (2): 85-91


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