EFFECT OF BARLEY SUPPLEMENT TO THE RATION OF DONOR ANIMALS USED IN IN VITRO DIGESTIBILITY DETERMINATION

1970 ◽  
Vol 50 (2) ◽  
pp. 265-267 ◽  
Author(s):  
F. W. CALDER
Keyword(s):  
Hordeum Vulgare ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Barley Grain ◽  
In Vitro Digestibility ◽  
Hordeum Vulgare L ◽  
Alfalfa Hay ◽  
Medicago Sativa L ◽  
Ad Libitum

Rumen fluid from two sheep was used to determine in vitro dry matter digestibilities of 25 samples each from alfalfa hay (Medicago sativa L.) and barley grain (Hordeum vulgare L.). The sheep were fed either a good quality hay ad libitum and 1 kg rolled barley per day, or only the hay ad libitum, in a switchback design. The in vitro dry matter digestibilities of both alfalfa hay and barley were lower (P < 0.01) when the donor animal was fed hay and barley than when fed only hay.

scholarly journals 49 Effects of in-vitro digestibility in cannulated steers when supplemented different levels of glycerol

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 55-56
Author(s):  
Noheli Gutierrez ◽  
Jamie A Boyd
Keyword(s):  
Dry Matter ◽  
Cold Water ◽  
Rumen Fluid ◽  
Latin Square ◽  
Optimal Level ◽  
Nutrient Digestibility ◽  
In Vitro Digestibility ◽  
Study Results ◽  
Different Levels

Abstract A study was conducted to evaluate effects of increasing concentration of food grade glycerol on rumen environment and nutrient digestibility. Three ruminally cannulated Jersey steers were used in this study. The study was conducted from March to May 2019. Experimental design was a 3x3 Latin square with a 2wk adjustment period followed by a 1wk collection period. Diet was coastal bermudagrass hay based. Different forage types were introduced in the incubation process to evaluate digestibility. Glycerol was administered once a day at 0, 15, or 20% of DMI (dry matter intake). dNDF (digestible NDF) and dDM (digestible dry matter) was determined using an ANKOM Daisy II incubator inoculated with 200g fresh rumen fluid and incubated for 12, 24, 48 and 72 h at 39°C. Each vessel contained ground forage samples in filter bags in triplicate. After incubation, filter bags were rinsed with cold water and dried for 24h in a 55°C forced air oven. Data were analyzed using the Proc MIXED procedure of SAS version 9.4. There was no difference dNDF in effect of different levels of glycerol between forage types by diet. But a numerical tendency was observed that dNDF was decreased at 20% inclusion rates in comparison to 0 and 15% inclusion of glycerol in the diet. Neither steer nor run was significantly different in the study. However as expected digestibility over time was significantly different (P &lt; 0.001). A significant increase was observed in DMI with the increased levels of glycerol in the diet (P = 0.003), both the 15% and 20% levels of glycerol increased in DMI in comparison to the control (0%). It appears based on these study results that digestibility may be inhibited, as levels of dietary glycerol increase in the diet and more work needs to be done to find the optimal level of glycerol supplementation.

Differences between the in vitro digestibility of extrusa collected from oesophageal fistulated steers and the forage consumed

2009 ◽  
Vol 49 (7) ◽  
pp. 563 ◽  
Author(s):  
David B. Coates ◽  
Robert J. Mayer
Keyword(s):  
Dry Matter ◽  
Rumen Fluid ◽  
In Vitro Digestibility ◽  
In Vitro Techniques ◽  
Pasture Legumes ◽  
Dry Matter Digestibility ◽  
Significant Difference ◽  
C3 Grasses

In a study that included C4 tropical grasses, C3 temperate grasses and C3 pasture legumes, in vitro dry matter digestibility of extrusa, measured as in vitro dry matter loss (IVDML) during incubation, compared with that of the forage consumed, was greater for grass extrusa but not for legume extrusa. The increase in digestibility was not caused by mastication or by the freezing of extrusa samples during storage but by the action of saliva. Comparable increases in IVDML were achieved merely by mixing bovine saliva with ground forage samples. Differences were greater than could be explained by increases due to completely digestible salivary DM. There was no significant difference between animals in relation to the saliva effect on IVDML and, except for some minor differences, similar saliva effects on IVDML were measured using either the pepsin–cellulase or rumen fluid–pepsin in vitro techniques. For both C4 and C3 grasses the magnitude of the differences were inversely related to IVDML of the feed and there was little or no difference between extrusa and feed at high digestibilities (>70%) whereas differences of more than 10 percentage units were measured on low quality grass forages. The data did not suggest that the extrusa or saliva effect on digestibility was different for C3 grasses than for C4 grasses but data on C3 grasses were limited to few species and to high digestibility samples. For legume forages there was no saliva effect when the pepsin–cellulase method was used but there was a small but significant positive effect using the rumen fluid–pepsin method. It was concluded that when samples of extrusa are analysed using in vitro techniques, predicted in vivo digestibility of the feed consumed will often be overestimated, especially for low quality grass diets. The implications of overestimating in vivo digestibility and suggestions for overcoming such errors are discussed.

Relative efficacy of inocula from rumen fluid or faecal solution for determining in vitro digestibility and gas production

1998 ◽  
Vol 78 (4) ◽  
pp. 673-679 ◽  
Author(s):  
A. El-Meadaway ◽  
Z. Mir ◽  
P. S. Mir ◽  
M. S. Zaman ◽  
L. J. Yanke
Keyword(s):  
Rumen Fluid ◽  
Relative Size ◽  
Barley Grain ◽  
Gas Production ◽  
Barley Straw ◽  
In Vitro Digestibility ◽  
Production Values ◽  
Cattle Faeces ◽  
Faecal Suspension

Three experiments were conducted to study the effects of substituting rumen fluid (RF) with faecal suspension (FS) as an inoculum for determination of in vitro dry matter digestibility (IVDMD, experiment 1) and gas production (exp. 2). Barley grain (BG), Persian clover (PC), alfalfa (ALF), bromegrass (BR) and barley straw (BS) were used to evaluate the efficacy of the two inocula. In exp. 1, IVDMD was determined using RF or FS containing 3, 6 and 9% fresh cattle faeces as inocula. Except for BS, IVDMD values obtained with either RF or FS containing 3% faeces were not different (P > 0.05). In contrast, FS containing 6 or 9% faeces resulted in lower (P < 0.05) IVDMD than those obtained with RF. Total VFA, butyric and valeric acids of in vitro supernatant were higher (P < 0.05) in RF than FS incubated buffers. Molar proportions of acetic and propionic acids were variable among feeds. Mean acetic:propionic ratio was similar (except for BG) for RF or FS. In exp. 2, substitution of RF with FS containing 6, 11 or 16% cattle faeces as inoculum generally resulted in an increased lag time for four feeds (PC was not included). The rate of gas production was lower when FS was used instead of RF for ALF and BR. For BG however, use of FS resulted in a higher (P < 0.05) rate of gas production than when RF was used. Total gas production values were similar within all the feeds regardless of the source of inoculum. In experiment 3, the relative size of the total and cellulolytic populations were higher for RF than for FS, with a greater diversity of genera of bacteria isolated from RF. Results from this study indicate that FS has the potential to be used instead of RF to obtain IVDMD and gas production for BG and forage hay but not for poor quality roughages such as BS. However, more research with FS is required to confirm these findings. Key words: IVDMD, gas production, rumen fluid, faecal suspension

Potential of walnut (Juglans regia) leave ethanolic extract to modify ruminal fermentation, microbial populations and mitigate methane emission

2020 ◽  
Vol 60 (9) ◽  
pp. 1189
Author(s):  
M. Sahebi Ala ◽  
R. Pirmohammadi ◽  
H. Khalilvandi-Behroozyar ◽  
E. Anassori
Keyword(s):  
Methane Emission ◽  
Rumen Fluid ◽  
Juglans Regia ◽  
Ethanolic Extract ◽  
Barley Grain ◽  
Gas Production ◽  
Microbial Populations ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation

Series of in vitro trials were conducted to evaluate dose–response effects of walnut leaf ethanolic extract (WLEE) on ruminal fermentation, microbial populations, mitigation of methane emission and acidosis prevention. The treatments were conducted according to a 5 × 3 factorial arrangement in a completely randomised design formulated to contain corn (corn-based diet, CBD) and barley grain (barley-based diet, BBD), or equal amounts of barley and corn (barley and corn diet, BCD), consisting of either basal diets alone (0) or basal diets with 250, 500, 750 or 1000 µL of WLEE (W0, W250, W500, W750 and W1000 respectively) per litre of buffered rumen fluid. Three fistulated cows fed diets containing alfalfa hay and concentrate mixes (same as the control diet) plus minerals and vitamins were used for collection of ruminal fluid. The asymptote of gas production and methane emission was decreased and lag time increased in a linear and quadratic manner with an increasing dose of WLEE (P &lt; 0.001). However, gas production rate reduced linearly as WLEE dose increased (P &lt; 0.001). Methane production was significantly reduced linearly (L) and quadratically (Q) when walnut ethanolic extract was increased from 250 to 1000 μL/L (L and Q; P &lt; 0.001). The addition of WLEE significantly altered the volatile fatty acid profile in comparison to control, reducing the molar proportion of acetate and increasing that of propionate (P &lt; 0.001), and also decreased the ammonia-N concentration (L, P &lt; 0.001). Dry-matter and organic-matter in vitro digestibility coefficients were negatively affected by WLEE supplementation (L and Q; P &lt; 0.001). Although anti-acidosis potential of WLEE was significantly lower than that of monensin, W1000 increased medium culture pH compared with uncontrolled acidosis and the lower doses of WLEE. The populations of Fibrobacter succinogenes, Ruminococcus flavefaciens and R. albus were significantly reduced by WLEE, although to different magnitudes, depending on the corn and barley grain proportions in the diet. Results of the present study indicated that increasing addition levels of WLEE have noticeable effects on rumen microbial population and fermentation characteristics. It can be concluded that WLEE can potentially be used to manipulate ruminal fermentation patterns.

EFFECTS OF NITROGEN APPLICATION ON BARLEY, OATS AND TRITICALE GROWN AS FORAGE

1975 ◽  
Vol 55 (1) ◽  
pp. 49-53 ◽  
Author(s):  
H. G. NASS ◽  
H. T. KUNELIUS ◽  
M. SUZUKI
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Stem Elongation ◽  
In Vitro Digestibility ◽  
Hordeum Vulgare L ◽  
Whole Plant ◽  
The Mean ◽  
Triticosecale Wittmack ◽  
X Triticosecale Wittmack

A 3-yr study was conducted to determine the effects of high rates of N fertilizer (NH4NO3) on whole plant dry matter yields, crude protein and NO3-N concentrations and in vitro digestibility of dry matter of oats (Avena sativa L.), barley (Hordeum vulgare L.) and triticale (X Triticosecale Wittmack) harvested at early dough stage. Dry matter (DM) yields of barley only were significantly increased by N applications over the zero N rate every year. Split applications of N at seeding and stem elongation did not generally result in higher DM yields than the corresponding single applications of N at seeding. The crude protein concentration ranged from 5.1 to 12.1% in the zero N check plot and from 7.1 to 14.9% in the N-fertilized plots. The NO3-N concentration in tissue was generally less than 0.10%, although NO3-N concentrations up to 0.34% were recorded in oats at 200–300 kg N/ha rates. For barley and triticale, the mean in vitro digestibility of DM was 57.2 and 58.3%, respectively, while the mean in vitro digestibility of DM for oats was much lower at 46.9%. In vitro digestibility of DM was affected very little by rates of N. The limitations in using cereals for forage are discussed.

QUALITY OF ALFALFA HERBAGE ESTIMATED BY A PREPARED CELLULASE SOLUTION AND NEAR INFRARED REFLECTANCE SPECTROSCOPY

1989 ◽  
Vol 69 (3) ◽  
pp. 833-839 ◽  
Author(s):  
S. S. BUGHRARA ◽  
D. A. SLEPER ◽  
R. L. BELYEA ◽  
G. C. MARTEN
Keyword(s):  
Dry Matter ◽  
Near Infrared ◽  
Reflectance Spectroscopy ◽  
In Vitro Digestibility ◽  
Infrared Reflectance ◽  
Near Infrared Reflectance Spectroscopy ◽  
Near Infrared Reflectance ◽  
Wide Range ◽  
Medicago Sativa L

Little information is available on estimating in vitro dry matter digestibility (IVDMD) of alfalfa (Medicago sativa L.) herbage by a prepared cellulase solution (PCS) and then using these IVDMD estimates to calibrate near infrared reflectance spectroscopy (NIRS) equations. Objectives were to compare PCS digestion to that by two rumen fermentation procedures, including true in vitro digestibility (TIVD), and develop NIRS equations to estimate TIVD, neutral detergent fiber, and acid detergent fiber of alfalfa hay. Seventy-eight alfalfa samples, having a wide range in herbage quality, were analyzed for IVDMD using five different PCS procedures and two rumen fermentation procedures (true and apparent in vitro digestibility). The best NIRS calibration equation for TIVD had R2 of 0.92 and a standard error of selection of 20.7 g kg−1. Correlations between IVDMD and TIVD obtained by the various PCS assays ranged from 0.91 to 0.96 (P < 0.01), with regression coefficients ranging from 0.94 to 0.98. We concluded that PCS gave rapid and accurate estimates of TIVD and that NIRS could accurately estimate TIVD of a wide range of alfalfa herbage quality.Key words: Acid detergent solubles, fungal cellulase solubles, in vitro digestible dry matter, Medicago sativa L., neutral detergent solubles, alfalfa

Comparison of rumen fluid from South African game species and from sheep to digest tanniniferous browse

2001 ◽  
Vol 52 (4) ◽  
pp. 453 ◽  
Author(s):  
R. J. Jones ◽  
J. H. F. Meyer ◽  
F. M. Bechaz ◽  
M. A. Stoltz ◽  
B. Palmer ◽  
...  
Keyword(s):  
Adverse Effects ◽  
South African ◽  
Dry Matter ◽  
Animal Species ◽  
Animal Feed ◽  
Rumen Fluid ◽  
Microbial Populations ◽  
In Vitro Digestibility ◽  
Rumen Bacteria

The hypothesis that South African game animals that use browse containing condensed tannins (CT) could have rumen microbial populations better able to ferment tanniniferous forages was explored. Rumen fluid (RF) from a range of browsing ruminants was used to ferment tanniniferous forages in vitro and the results were compared with the values obtained with rumen fluid from sheep. Leaves of the shrub legumes Leucaena leucocephala, L. pallida, L. trichandra, Calliandra calothyrsus,Gliricidia sepium, and Acacia boliviana were used as substrates. In vitro digestibility of dry matter (IVDMD) and nitrogen (IVND) was measured in the presence or absence of polyethylene glycol (PEG) 4000 to assess the adverse effects of the CT in the feeds. Rumen fluid from giraffe, greater kudu, eland, duiker, impala, nyala, goat, and gnu was compared with that from sheep. Ability to metabolise or block the adverse effects of CT was expected to result in only a small or no response to PEG in terms of IVDMD and IVND with rumen fluid from browsers, and therefore to result in a significant animal feed and animal PEG interaction. There were no significant (P > 0.05) animal feed interactions. For every animal species there was a response to PEG in terms of IVND, and this response was linearly related to the CT in the feed as measured by PEG binding using 14C-labelled PEG. Rumen fluid from browsers had a lower pH (5.78 0.060 v. 6.68 0.052) and a higher dry matter content (1473 102 mg v. 415 4.3 mg/100 mL RF) than that of grazers (sheep and gnu). However, when measured at 2 pH levels (6.7 and 5.8), IVDMD and IVND of the feeds were not improved at the lower pH with rumen fluid from the browsers. Rather, digestibility was depressed at the lower pH with rumen fluid from all animal species. We conclude from these in vitro studies that rumen microbial populations from browsers are not able to ferment tanniniferous forages better than rumen microbial populations from grazers. It seems likely that these browsers do not have rumen bacteria capable of degrading the CT in these shrub legumes. Production on such tanniniferous feeds may well depend on tannin binding with proline-rich saliva rather than on metabolism of or tolerance to CT by rumen bacteria.

scholarly journals Production of alfalfa hay under different drying methods

2010 ◽  
Vol 39 (8) ◽  
pp. 1676-1683 ◽  
Author(s):  
Marcela Abbado Neres ◽  
Deise Dalazen Castagnara ◽  
Eduardo Eustáquio Mesquita ◽  
Maximilliane Alavarze Zambom ◽  
Leiliane Cristine de Souza ◽  
...  
Keyword(s):  
Cell Wall ◽  
Crude Protein ◽  
Dry Matter ◽  
In Vitro Digestibility ◽  
Drying Methods ◽  
Drying Process ◽  
Dry Matter Digestibility ◽  
Alfalfa Hay ◽  
Number Of Leaves

This trial aimed at determining the dehydration curve at nine times (0, 3, 6, 9, 24, 27, 30, 33 and 46 hours after cuting of the plants); the leaf/stem ratio and number of leaves per stem in alfalfa submitted to four managements during the drying process. It was also evaluated the bromatologic composition and in vitro digestibility of the dry matter and cell wall of alfalfa hay before storage. The drying methods applied for alfalfa were the following: drying in the field until 50% DM and drying conclusion in an airy shed; drying in the field without turning; drying with only one turning and drying with two turnings. The experimental design was completely randomized with four drying methods and five replications. Leaf dehydration was faster when compared to the stems in all drying methods. Drying in the shed promoted the lesser re-hydration of the plants at night due to dew. It was also verified that alfalfa hays that remained under the sun and were turned showed the worst quality with reduction in crude protein contents and increase in NDF, ADF, NDIN and ADIN. However, in vitro dry matter digestibility and cell wall digestibility did not suffer any alteration because of the different dehydration methods (69.79 and 41.39%, respectively). There is a market fall of leaves and a reduction on the number of leaves per stem with the turnings.

scholarly journals Effect of replacing alfalfa hay with Leucaena leucocephala (L. Leucocephala) leaves on in vitro gas production, digestibility and in situ degradability in buffalo

2021 ◽  
Vol 43 ◽  
pp. e52129
Author(s):  
Tahereh Mohammadabadi ◽  
Morteza Chaji ◽  
Ehsan Direkvandi ◽  
Othman Alqaisi
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Replacement Rate ◽  
Alfalfa Hay ◽  
In Vitro Gas Production ◽  
Dietary Treatments

. This study was performed to investigate the effect of replacing alfalfa hay by L. leucocephala leaves in proportions of 25, 50 and 100% on in vitro gas production (GP) parameter, digestibility and in situ degradability in buffalo. Results showed that the volume of GP at 2 to 12 hours after incubation was significantly affected by replacing alfalfa hay with L. leucocephala leaves. In vitro digestibility of organic matter (OMD) differed significantly between treatment as it declined by increasing the alfalfa hay substitution rate from 25 to 100%. The microbial crude protein (MCP) differed significantly between treatments and was the greatest of 589 and 599 mg g-1 of dry matter (DM) when L. leucocephala leaves replaced alfalfa hay at 25 and 50%. The in vitro digestibility of DM (IVDMD) increased significantly at 50% L. leucocephala replacement rate. Moreover, substituting alfalfa hay by L. leucocephala had a significant effect on the in situ degradability parameters. The insoluble but potentially degradable fraction (B) and potential of degradability (A+B) significantly increased for treatment contain 50% L. leucocephala leaves. The effective degradability (ED) was significantly different between dietary treatments and was the greatest when alfalfa hay was replaced by 25 and 50% L. leucocephala. In conclusion, L. leucocephala leaves can substitute 25 to 50% of dietary alfalfa hay in buffalo rations without effect on rumen efficiency.

INTERACTION BETWEEN INOCULUM DONOR DIET AND SUBSTRATE IN IN VITRO RUMINANT DIGESTION STUDIES

1966 ◽  
Vol 46 (2) ◽  
pp. 91-95 ◽  
Author(s):  
J. E. Knipfel ◽  
J. E. Troelsen
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
In Vitro Digestion ◽  
Barley Grain ◽  
Absolute Amount ◽  
In Vitro Digestibility ◽  
Promoting Effect ◽  
Digestion Technique ◽  
The Difference

Six wethers with rumen fistula were each fed a different combination of alfalfa hay, wheat straw, and barley grain. The same mixtures were fermented for periods of 12 and 48 hours by inoculum from each wether, using a two-stage in vitro digestion technique. The in vitro digestibility of dry matter and organic matter was determined on each treatment combination.The digestibility data revealed interactions among inoculum donor diets, in vitro substrate mixtures, and fermentation periods. When the diet lacked one or more of the substrate components, a 12-hour artificial rumen fermentation resulted in digestibilities that decreased as the composition of the substrate deviated increasingly from that of the donor diet. This effect was eliminated by extending the fermentation period to 48 hours. When all the substrate ingredients were included in the donor diet, the absolute amount of each ingredient, within 10 to 80%, was of less importance.Indications were that alfalfa possessed a specific growth-promoting effect on the microflora.Increasing the proportion of alfalfa in the substrate increased the difference between the in vitro digestibility of dry matter and organic matter. This was suggested to result from a higher concentration of soluble ash in the alfalfa fraction of the mixtures.

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