Synthesis and evaluation of an alginate-methacrylate xerogel for insulin delivery towards wound healing applications

2021 ◽  
Vol 12 (3) ◽  
pp. 215-234
Author(s):  
GP Rajalekshmy ◽  
MR Rekha
Keyword(s):  
Wound Healing ◽  
Transmission Rate ◽  
Wound Care ◽  
Physical Stability ◽  
Analytical Techniques ◽  
Insulin Delivery ◽  
Water Vapour Transmission Rate ◽  
Scratch Wound ◽  
Scratch Wound Assay

Background: Alginate is one of the most widely used biopolymer for wound healing. But poor mechanical strength and degradability limits its application especially as a drug-delivery matrix. The aim of this study was to develop stable alginate based scaffold for insulin delivery toward wound care. Materials & methods: The xerogel alginate-g-poly (methacrylic acid; AGM2S) was characterized by various analytical techniques. Results: AGM2S xerogel showed improved physical stability, low degradation, good swelling and water vapour transmission rate (WVTR). About 70% of insulin was released from loaded xerogel over a period of 48 h and favorably modulated the healing response in in vitro scratch wound assay. Conclusion: Grafting improved the strength and stability of alginate xerogel and the results suggest the application of insulin loaded AGM2S xerogels as a potential wound healing material.

scholarly journals The Activity of Combination of Hydrolyzed Virgin Coconut Oil and Chitosan Toward Wound Healing Parameters on NIH 3T3 Cells Using in Vitro Methods

1970 ◽  
Vol 7 (3) ◽  
pp. 14-19 ◽  
Author(s):  
Hekdin Marsius Sipayung ◽  
Jansen Silalahi ◽  
Yuandani Y
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Protein Expression ◽  
Scratch Wound ◽  
Cell Proliferation Activity ◽  
Proliferation Activity ◽  
Cox 2 ◽  
Nih 3T3 ◽  
Vegf Protein

Objectives: The objective of this study was to investigate the activity of combination of hydrolyzed VCO (HVCO) and chitosan on NIH 3T3 cell proliferation activity, NIH 3T3 cell migration, COX-2 and VEGF protein expression. Design: In vitro cytotoxic assay was determined by MTT (MicrocultureTetrazoliumTehnique) assay, cell proliferation activity was measured by calculating cell viability incubated 24 hours, 48 hours and 72 hours, wound closure percentage was tested by scratch wound healing method, expression of COX-2 protein and VEGF protein were measured by immunocytochemical method. Interventions: The variable that was intervened in this study was the concentration of HVCO and chitosan. Main Outcome Measures: The main measurements carried out in this study were the absorbance value of HVCO and chitosan which was converted into viability cell, proliferation activity, percentage of wound closure, and percentage of COX-2 and VEGF protein expression. Results: Cytotoxic activity of HVCO and chitosan resulted the best concentration at 31.25 μg/ml, scratch wound healing assay from a combination HVCO and chitosan resulted the best migration of fibroblast cells at a ratio of 1:1 with HVCO 62.5 μg/ml and chitosan 62.5 μg/ml, combination of HVCO 62.5 μg/ml and chitosan 62.5 μg/ml (1:1) increased expression of COX-2 and VEGF. Conclusion: Combination of HVCO and chitosan could increase NIH 3T3 cell migration, COX-2 and VEGF protein expression. Combination of HVCO and chitosan had better wound healing activity in vitro than single use. Keywords: Rhizomucor miehei, viability, proliferation, migration, expression

Multifunctional Zinc Oxide/Silver Bimetallic Nanomaterial-Loaded Nanofibers for Enhanced Tissue Regeneration and Wound Healing

2021 ◽  
Vol 17 (9) ◽  
pp. 1840-1849
Author(s):  
Mao Li ◽  
Min Hu ◽  
Honglian Zeng ◽  
Bo Yang ◽  
Yi Zhang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Zinc Oxide ◽  
Tissue Regeneration ◽  
Ag Nanoparticles ◽  
Wound Care ◽  
Fluid Handling ◽  
Skin Repair ◽  
Wound Healing Effect

Native skin repair requires wound care products that not only protect the wound from bacterial infection, but also accelerate wound closure and minimize scarring. Nanomaterials have been widely applied for wound healing due to their multifunctional properties. In a previous study, we prepared and characterized electrospinning zinc oxide/silver/polyvinylpyrrolidone/polycaprolactone (ZnO/Ag/PVP/PCL) nanofibers using ZnO and Ag nanoparticles, and evaluated their antibacterial effect in vitro. In this work, further characterization studies were performed, which confirmed that the ZnO/Ag nanoparticles were physically embedded and evenly distributed in the ZnO/Ag/PVP/PCL nanofibers, enabling the sustained release of Ag and Zn. In addition, the bimetallic nanofibers showed satisfactory fluid handling and flexibility. In vivo wound healing and histology studies showed that the ZnO/Ag/PVP/PCL nanofibers had a better anti-inflammatory, skin tissue regeneration, and wound healing effect than monometallic nanofibers or a commercially available wound plaster (Yunnan Baiyao). Therefore, ZnO/Ag/PVP/PCL bimetallic nanofibers may be a safe, efficient biomedical dressing for wound healing.

Auswirkungen Konservierungsmittel-freier Fluorchinolon-Augenlösungen auf menschliche Hornhaut-Epithelzellen in vitro

2015 ◽  
Vol 1 (1) ◽  
pp. 26-27
Keyword(s):  
Scratch Wound ◽  
Scratch Wound Assay

Ziele: Die Beurteilung der biologischen Wirkungen Konservierungsmittel-freier Fluorchinolon-Augenlösungen auf Zellkulturen menschlichen Hornhaut-Epithels in vitro.Methoden: Wir untersuchten die Auswirkung von topischen Fluorchinolonen verschiedener Generationen, wie Ofloxacin 0,3%, Levofloxacin 0,5%, Tosufloxacin 0,3%, Moxifloxacin 0,5% und Gatifloxacin 0,3%, auf gezüchtete menschliche Hornhaut-Epithelzellen. Die Untersuchung erfolgte mittels MTT-basiertem kalorimetrischem Assay, Laktatdehydrogenase(LDH)-Leakage-Assay und Scratch-Wound-Assay. Die Morphologie der Hornhaut-Epithelzellen wurde mittels inverser Lichtmikroskopie und Transmissionselektronenmikroskopie untersucht.Ergebnisse: Bei allen topischen Fluorchinolonen ging die metabolische Aktivität der Hornhaut-Epithelzellen zeitabhängig zurück, und der LDH-Titer stieg mit zunehmender Dauer der Wirkstoffexposition. Insbesondere nach einer Exposition gegenüber Moxifloxacin 0,5% und Gatifloxacin 0,3% war ein signifikanter Anstieg der LDH-Titer im Vergleich zu den Kontrollen festzustellen. Die Migrationsraten der Hornhaut-Epithelzellen waren bei Ofloxacin 0,3% und Levofloxacin 0,5% höher als bei den anderen Fluorchinolonen. Nach einer Exposition gegenüber Moxifloxacin 0,5% und Gatifloxacin 0,3% waren schwere morphologische Schäden an den Zellen zu beobachten.Schlussfolgerung: Da Moxifloxacin 0,5% und Gatifloxacin 0,3% eine stärkere toxische Wirkung auf die Hornhaut-Epithelzellen ausübten als die anderen Fluorchinolone, sind diese Fluorchinolon-Augenlösungen der 4. Generation nur nach sorgfältiger Abwägung im Hinblick auf die mögliche Schädigung des Hornhaut-Epithels bei langer Behandlungsdauer oder zu hoher Dosierung anzuwenden.Übersetzung aus Ophthalmic Res 2014;51:216-223 (DOI: 10.1159/000357976)

scholarly journals Electrospun PCL/PLA Scaffolds Are More Suitable Carriers of Placental Mesenchymal Stromal Cells Than Collagen/Elastin Scaffolds and Prevent Wound Contraction in a Mouse Model of Wound Healing

2020 ◽  
Vol 8 ◽  
Author(s):  
Eva Vonbrunn ◽  
Marc Mueller ◽  
Melanie Pichlsberger ◽  
Monika Sundl ◽  
Alexander Helmer ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mouse Model ◽  
Stromal Cells ◽  
Wound Care ◽  
Skin Wound ◽  
Beneficial Effects ◽  
New Therapeutic Approaches

Mesenchymal stem/stromal cells (MSCs) exert beneficial effects during wound healing, and cell-seeded scaffolds are a promising method of application. Here, we compared the suitability of a clinically used collagen/elastin scaffold (Matriderm) with an electrospun Poly(ε-caprolactone)/poly(l-lactide) (PCL/PLA) scaffold as carriers for human amnion-derived MSCs (hAMSCs). We created an epidermal-like PCL/PLA scaffold and evaluated its microstructural, mechanical, and functional properties. Sequential spinning of different PCL/PLA concentrations resulted in a wide-meshed layer designed for cell-seeding and a dense-meshed layer for apical protection. The Matriderm and PCL/PLA scaffolds then were seeded with hAMSCs, with or without Matrigel coating. The quantity and quality of the adherent cells were evaluated in vitro. The results showed that hAMSCs adhered to and infiltrated both scaffold types but on day 3, more cells were observed on PCL/PLA than on Matriderm. Apoptosis and proliferation rates were similar for all carriers except the coated Matriderm, where apoptotic cells were significantly enhanced. On day 8, the number of cells decreased on all carrier types except the coated Matriderm, which had consistently low cell numbers. Uncoated Matriderm had the highest percentage of proliferative cells and lowest apoptosis rate of all carrier types. Each carrier also was topically applied to skin wound sites in a mouse model and analyzed in vivo over 14 days via optical imaging and histological methods, which showed detectable hAMSCs on all carrier types on day 8. On day 14, all wounds exhibited newly formed epidermis, and all carriers were well-integrated into the underlying dermis and showing signs of degradation. However, only wounds treated with uncoated PCL/PLA maintained a round appearance with minimal contraction. Overall, the results support a 3-day in vitro culture of scaffolds with hAMSCs before wound application. The PCL/PLA scaffold showed higher cell adherence than Matriderm, and the effect of the Matrigel coating was negligible, as all carrier types maintained sufficient numbers of transplanted cells in the wound area. The anti-contractive effects of the PCL/PLA scaffold offer potential new therapeutic approaches to wound care.

scholarly journals An image J plugin for the high throughput image analysis of in vitro scratch wound healing assays

2020 ◽  
Author(s):  
Alejandra Suarez-Arnedo ◽  
Felipe Torres Figueroa ◽  
Camila Clavijo ◽  
Pablo Arbeláez ◽  
Juan C. Cruz ◽  
...  
Keyword(s):  
Wound Healing ◽  
Image Analysis ◽  
Low Cost ◽  
Cellular Migration ◽  
Wound Healing Assay ◽  
Wound Area ◽  
Scratch Wound ◽  
Scratch Wound Healing Assay ◽  
Imagej Plugin

AbstractIn vitro scratch wound healing assay, a simple and low-cost technique that works along with other image analysis tools, is one of the most widely used 2D methods to determine the cellular migration and proliferation in processes such as regeneration and disease. There are open-source programs such as imageJ to analyze images of in vitro scratch wound healing assays, but these tools require manual tuning of various parameters, which is time-consuming and limits image throughput. For that reason, we developed an optimized plugin for imageJ to automatically recognize the wound healing size, correct the average wound width by considering its inclination, and quantify other important parameters such as: area, wound area fraction, average wound width, and width deviation of the wound images obtained from a scratch/ wound healing assay. Our plugin is easy to install and can be used with different operating systems. It can be adapted to analyze both individual images and stacks. Additionally, it allows the analysis of images obtained from bright field, phase contrast, and fluorescence microscopes. In conclusion, this new imageJ plugin is a robust tool to automatically standardize and facilitate quantification of different in vitro wound parameters with high accuracy compared with other tools and manual identification.

scholarly journals Development of Bioinspired Gelatin and Gelatin/Chitosan Bilayer Hydrofilms for Wound Healing

Pharmaceutics ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 314 ◽  
Author(s):  
Itxaso Garcia-Orue ◽  
Edorta Santos-Vizcaino ◽  
Alaitz Etxabide ◽  
Jone Uranga ◽  
Ardeshir Bayat ◽  
...  
Keyword(s):  
Wound Healing ◽  
Citric Acid ◽  
Wound Dressing ◽  
Ex Vivo ◽  
Lower Layer ◽  
Transmission Rate ◽  
Dry Weight ◽  
Porous Matrix ◽  
In Vitro Cytotoxicity

In the current study, we developed a novel gelatin-based bilayer wound dressing. We used different crosslinking agents to confer unique properties to each layer, obtaining a bioinspired multifunctional hydrofilm suitable for wound healing. First, we produced a resistant and non-degradable upper layer by lactose-mediated crosslinking of gelatin, which provided mechanical support and protection to overall design. For the lower layer, we crosslinked gelatin with citric acid, resulting in a porous matrix with a great swelling ability. In addition, we incorporated chitosan into the lower layer to harness its wound healing ability. FTIR and SEM analyses showed that lactose addition changed the secondary structure of gelatin, leading to a more compact and smoother structure than that obtained with citric acid. The hydrofilm was able to swell 384.2 ± 57.2% of its dry weight while maintaining mechanical integrity. Besides, its water vapour transmission rate was in the range of commercial dressings (1381.5 ± 108.6 g/m2·day). In vitro, cytotoxicity assays revealed excellent biocompatibility. Finally, the hydrofilm was analysed through an ex vivo wound healing assay in human skin. It achieved similar results to the control in terms of biocompatibility and wound healing, showing suitable characteristics to be used as a wound dressing.

Challenges with Wound Infection Models in Drug Development

2020 ◽  
Vol 21 (13) ◽  
pp. 1301-1312 ◽  
Author(s):  
Sandeep K. Shukla ◽  
Ajay K. Sharma ◽  
Vanya Gupta ◽  
Aman Kalonia ◽  
Priyanka Shaw
Keyword(s):  
Wound Healing ◽  
Wound Infection ◽  
Chronic Wound ◽  
Wound Care ◽  
Infection Model ◽  
In Vivo Models ◽  
Infection Models

: Wound research is an evolving science trying to unfold the complex untold mechanisms behind the wound healing cascade. In particular, interest is growing regarding the role of microorganisms in both acute and chronic wound healing. Microbial burden plays an important role in the persistence of chronic wounds, ultimately resulting in delayed wound healing. It is therefore important for clinicians to understand the evolution of infection science and its various etiologies. Therefore, to understand the role of bacterial biofilm in chronic wound pathogenesis, various in vitro and in vivo models are required to investigate biofilms in wound-like settings. Infection models should be refined comprising an important signet of biofilms. These models are eminent for translational research to obtain data for designing an improved wound care formulation. However, all the existing models possess limitations and do not fit properly in the model frame for developing wound care agents. Among various impediments, one of the major drawbacks of such models is that the wound they possess does not mimic the wound a human develops. Therefore, a novel wound infection model is required which can imitate the human wounds. : This review article mainly discusses various in vitro and in vivo models showing microbial colonization, their advantages and challenges. Apart from these models, there are also present ex vivo wound infection models, but this review mainly focused on various in vitro and in vivo models available for studying wound infection in controlled conditions. This information might be useful in designing an ideal wound infection model for developing an effective wound healing formulation.

scholarly journals Revealing the Mechanism ofIn VitroWound Healing Properties ofCitrus tamuranaExtract

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Madhyastha Harishkumar ◽  
Yamaguchi Masatoshi ◽  
Sameshima Hiroshi ◽  
Ikenoue Tsuyomu ◽  
Maruyama Masugi
Keyword(s):  
Cell Cycle ◽  
Wound Healing ◽  
De Novo ◽  
Cyclin Dependent Kinases ◽  
Scratch Wound ◽  
Dependent Cell ◽  
Expression Activity ◽  
And Migration ◽  
Migration Of Cells

In the present investigation, we examined the effect of Hyuganatsu (Citrus tamurana) extract (HE) on skin fibroblast (TIG-119) proliferation and migration duringin vitrowound healing. HE selectively inhibited proliferation of TIG-119 cells at higher concentration (>1.0 mg/mL); at lower concentrations (0.1, 0.25, 0.5, and 0.75 mg/mL), it exhibited linear and time-dependent cell proliferation.In vitroscratch wound healing studies showed that the HE also accelerated the migration of cells towards the wounded region. Cytometric analysis demonstrated that HE extract did not alter G1/0 and S phases of cell cycle in any concentration studied; however, G2/M phases of cell cycle were significantly (P<0.05) accelerated at 0.75 mg/mL dose. RT-PCR and Western blotting analysis indicated that HE markedly overexpressed levels of Rac-1, Rho-A, and Cdc-42 mRNA and the respective proteins. Cyclin-dependent kinases (Cdk-1 and -2) gene expression activity was significantly (P<0.05) increased, but protein content decreased during treatment with HE. The induction of Cdk-1 and -2 by HE was abolished by inhibitors, transcription (DRB), and translation (CHX), implying transcriptional regulation that requiredde novoprotein synthesis.

scholarly journals Propolis Induces AQP3 Expression: A Possible Way of Action in Wound Healing

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1544 ◽  
Author(s):  
Simona Martinotti ◽  
Giorgia Pellavio ◽  
Umberto Laforenza ◽  
Elia Ranzato
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Scientific Information ◽  
Folk Medicine ◽  
Pronounced Increase ◽  
Migration Assay ◽  
Positive Effects ◽  
Scratch Wound ◽  
A Cell

Propolis is the generic name of a complex of resinous compound collected by honeybees and it has been utilized for many years in folk medicine. As other products generated by honeybees (such as royal jelly, pollen, honey), propolis has great therapeutic properties, but very little scientific information is available. Therefore, this study was aimed at exploring the potential wound healing properties of propolis. To that end, we utilized an in vitro scratch wound healing model consisting of human immortalized keratinocytes. Our scratch wound data clearly demonstrated that propolis induced a pronounced increase in the wound repair abilities of keratinocytes. A cell migration assay showed that propolis stimulated keratinocytes to close the wound. We revealed the role of H2O2 as the main mediator of propolis regenerative properties. We showed that this extracellularly released H2O2 could pass across the plasma membrane through a specific aquaporin (i.e., AQP3) modulating intracellular responses. The data offer a biological characterization of propolis positive effects suggesting that propolis could also be utilized in wound treatment within clinical settings.

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