scholarly journals D-vitamin status of cattle depending on the conditions of detention and physiological state

2018 ◽  
Vol 20 (88) ◽  
pp. 141-146
Author(s):  
L. L. Yuskiv ◽  
V. Vlizlo

The article contains data on the content of active metabolite of vitamin D3 – 25OHD3, calcium, phosphorus, magnesium and activity of alkaline phosphatase in the blood of cows and their calves in the early postnatal period. The experiment was conducted on cows-analogues of the Ukrainian Black-and-White dairy breed during the dry period and after calving and on calves received from them. The experiment was performed during the winter period of the stall at the Lviv region farm. The blood for tests was collected from the jugular vein before morning feeding at 3–5 days before calving and in the 5th–7th and 55th–60th days after calving. The blood from the calves was collected in the following dates: on the first day and at 5th–7th days old and at 55–60th days old.  It was established that, the content of 25-hydroxycolecalciferol in blood calves on the first day after birth was 18.90 ± 2.08 nmol/l. In subsequent periods of study, its level decreased, in particular, at 5th−7th days old it was 1.52 times lower (P < 0.05), and in the at 55–60th days old – 2.07 times (P < 0.01) than on the first day old age during the winter period of the stall. Changes in the content of 25OHD3, total calcium and its fractions, inorganic phosphorus, magnesium and activity of alkaline phosphatase and its isoenzymes in the blood of cows in the prenatal and lactation periods have been determined. In particular, the content of 25OHD3 was 18.7 ± 2.27 nmol/l at 3–5 days before calving in the blood of cows, on the 5–7th day after calving continued to decrease and amounted to 15.8 ± 0.83 nmol/l, and on the 55–60th day of the postpartum period it increased to 20.5 ± 2.08 nmol/l.

2017 ◽  
Vol 19 (78) ◽  
pp. 136-140
Author(s):  
L.L. Yuskiv

The article presents data about the content of active metabolite of vitamin D3 – 25OHD3, calcium (total, bounded with protein and ultrafiltrated), inorganic  phosphorus, magnesium and alkaline phosphatase activity and its isoenzymes in the blood of cows and their calves in the early postnatal period.Studies were conducted in the cows of the Ukrainian Black-and-White dairy breed and calves obtained from them. The experiment was performed during the winter housing period in pilot farm «Pasichna» of Institute of forage and agricultural Podillya NAAS of Ukraine, located in the natural geographical areas of Podillya. The blood for tests was collected from the jugular vein before morning feeding in the 5th−7th day since calving. The blood from  the calves was collected in the following dates: at 5th−7th days old and at 28–30th days old. Vitamin D provision rate of animal organism was evaluated for the content of 25OH D3 concentration in blood. The level of 25-hydroxycholecalciferol is considered as a total reflection of the endogenous formation of cholecalciferol in the skin and its receipts from feed or vitaminpreparations. The concentration of 25ОНD3 in the blood of the examined animals was detected by means of the enzymelinked immunoassay using the test system developed by the Immunodiagnostik. The method is based on the competitive binding of 25OHD3 serum and 25OH D3-biotin with vitamin D3-binding protein (VDBP), that immobilized on 96-well immunological plates. The content of calcium (total, bounded with protein and ultrafiltrated), inorganic  phosphorus, magnesium and alkaline phosphatase (ALP) activity were detected usingthe biological test kits produced by the Pliva Lachema firm (the Czech Republic). The performed research reported that the content of 25-hydroxycholecalciferol in serum of calves at 5–7-days age old was in the range of 29 to 34 nmol/l and slightly increased for the 28–30-days. It was established the dynamics of changes in the content of total calcium and its fractions, inorganic phosphorus, magnesium and activity of alkaline phosphatase and its isoenzymes in calves blood from 5–7- to 28–30-day-olds. The concentration of 25-hydroxyvitamin D3 in the blood of calves in the first days after birth was lower, and the concentration of calcium and inorganic phosphorus – higher compared with their values in the blood of mothers in the postpartum period. It was found that the activity of alkaline phosphatase in the calf serum was higher, compared with its value in the serum of mothers. The increase in activity of alkaline phosphatase in the calf serum is expressed to a greater extent due to its bone enzyme. 


2014 ◽  
Vol 1 (1) ◽  
pp. 42-46
Author(s):  
L. Yuskiv ◽  
V. Vlizlo

Aim. To investigate the vitamin D status in highly productive cows during winter housing period and effect of cholecalciferol by various ways of vitamin D 3 injection to cows in last days of gestation and after calving. Methods. Enzyme-linked immunoassay, spectrophotometry. Results. It has been stated that intramuscular injection of cholecalciferol into cows caused increase of the vitamin D 3 active metabolite – 25-OHD 3 , calcium, phosphorus and magnesium levels together with decrease of alkaline phosphatase level in pre- and post-natal periods. Oral supplementation makes little infl uence on the studied blood parameters of cows. Conclusions. Extrabuccal administration and oral supplementation of cholecalciferol in winter housing period to high-yield cows in the last days of gestation and after calving is accompanied by increased levels of its metabolites and their effect on mineral metabolism in the postnatal period. The nature of these changes depends on the mode of vitamin D administration and the physiological state of the cows.


2019 ◽  
Vol 21 (93) ◽  
pp. 121-124
Author(s):  
D. V. Hrudetska ◽  
M. R. Simonov ◽  
O. O. Dashkovskyy

Recently, a significant amount of data has been reported indicating on the link between consumption of dairy products and development of oncological diseases. This could be explained by the presence in milk of various hormones, particularly insulin-like growth factor (IGF). This hormone is a mediator between somatotropin and tissues. In response to the increase of the blood level of growth hormone, hepatocytes begin actively synthesize the IGF, which accelerates the rate of the metabolism, including those of onco-cells. The majority of studies relate to humane medicine, and data on the dependence of IGF milk levels on breed, period of lactation, diet, physiological, and clinical status of an animal are lacking. Taking this into consideration, the purpose of this study was to analyze the concentration of insulin-like factor in colostrum, milk of cows and blood plasma at various stages of lactation. Study was performed on cows of Ukrainian black-and-white dairy breed in their from second to fifth lactation with milk yield 5100–5700 kg per previous lactation. Concentration of IGF was detected in colostrum, milk and plasma of cows, using enzyme immunoassay. Obtained material gives evidence that the highest IGF level is present in colostrum. After that hormone concentration decreases up to 10–14 day of lactation. High level of IGF in colostrum might be associated with necessity to stimulate metabolism of calves in early postnatal period of ontogenesis. Immediately after calving plasma level of IGF decreases. The highest level was observed on 10–14th days of lactation. Taking into consideration physiological function of the given hormone, there is no univocal explanation of the decrease of its plasma level in cows at the beginning of lactation. This could be associated with energy deficit, physiological condition of animals or with developing of lactation dominant and levels of other hormones. Potential of further research lies in investigation of the dependence of the IGF level in the secretion of the mammary gland on productivity, breed, age and diet of animals.


2019 ◽  
pp. 18-28

This study aims to assess vitamin D levels in women with postmenopausal osteoporosis and healthy women going through menopause. Two groups of menopausal women took part in the study – 41 women with osteoporosis and 22 without osteoporosis. The levels of vitamin D, parathormone, alkaline phosphatase, calcium and phosphorus were examined during the autumn-winter period. Calcium, phosphorus and alkaline phosphatase were established within the reference range in both groups. A negative correlation with increase of parathormone levels among the patients with osteoporosis and vitamin D deficiency was found (r = -0.46, p<0.01). 16 women with osteoporosis (39%) were with normal levels of vitamin D, 14 (34,1%) were with insufficiency and 11 (26,9%) with deficiency. Within the control group, 8 women (36,4%) were with normal levels of vitamin D, 12 (54,5%) were with insufficiency and 2 (9,1%) with deficiency.


Author(s):  
Yu. O. Schepetilnicov ◽  
◽  
Yu. V. Maslak ◽  
O. V. Matsenko ◽  
V. A. Pasichnik ◽  
...  

The productivity of cows is related to the level of metabolism in animals. The intensity of metabolic processes depends on the physiological state of the animals. The aim of this study was to establish the metabolic profile of blood serum in cows according to different physiological groups. The object of the study was 15 cows of the Ukrainian black and white dairy breed kept in the HDZVA. There were 5 animals of the milking period, 5 in dry period and 5 lactating (6 months after calving). The animals were examined by general clinical methods (temperature, pulse, and respiratory rate were determined) and blood was taken from the jugular vein for biochemical research. Content of total protein, albumins, globulins, urea, creatinine, total cholesterol, the concentration of glucose and the activity of liver-indicator enzymes - alanine and aspartic transaminases (ALT, ASAT) were determined in blood serum. Blood samples (n = 15) were examined by the following methods: protein content was determined using the biuret reaction, the concentration of albumins and globulins using precipitation with saline solutions, urea – by the method with diacetyl monooxime, creatinine – by the kinetic reaction, cholesterol – by the Jendrashik method, glucose – by glucoseoxidase reaction, activity of transaminases according to Raitman-Frenkel. Numerical data were statistically processed using standard «Microsoft Excel» programs, using Student’s t-criteria. In cows after parturition the level of total protein in blood serum was lower by 7.9% compared with the norm. The concentration of albumins was reduced in lactating animals after parturition and the period of six months after calving by 19.5 % and 20.9 %, respectively. This indicates a decrease in the protein-forming function of the liver. Concentration of cholesterol in blood serum of lactating animals was lower by 34.9%, which may indicate both a lack of energy resources in the diet of animals and a malfunction of the thyroid gland. We recommend to investigate the nutritional value of all feeds used on the farm for feeding cattle, in order to exclude imbalances in the diet in terms of content and balance of nutrients.


1971 ◽  
Vol 49 (4) ◽  
pp. 338-344 ◽  
Author(s):  
I. Motzok ◽  
Gwendolyn M. Ritcey ◽  
Muriel I. Davies

Diets with varying levels of Ca, inorganic phosphorus (P1), and vitamin D3 were fed to chicks from 1 day of age. Alkaline phosphatase of intestinal mucosa was twofold higher at 3 weeks in female chicks fed 0.16% of P1 than in chicks fed 0.48% of P1 when the diets contained 7500 I.C.U. of vitamin D3 per kilogram and 1% of Ca. The effect of low dietary P1 was less pronounced when the diets contained 450 I.C.U. of vitamin D3 per kilogram and 1% of Ca but no definite pattern in enzyme activities due to level of P1 was observed with 0.53% of Ca. In male chicks there was a small but consistent increase (35–45%) in the activity of intestinal phosphatase when the diets were low in Ca, P1, or both. The level of vitamin D3 had no effect on the intestinal enzyme in male birds.


2019 ◽  
Vol 21 (96) ◽  
pp. 124-128
Author(s):  
L. L. Yuskiv ◽  
V. V. Vlizlo ◽  
N. R. Motko

The article contains data on the content of the active metabolite of vitamin D3 – 25-OHD3, the concentration of calcium, phosphorus, magnesium and the activity of alkaline phosphatase in the blood of lactating cows in different periods of keeping. The experiment was performed by the method of periods on the cows-analogues for the 4th month of lactation. Cows are divided into three groups of five heads, each depending on the sampling period: winter stall (January), summer pasture (July) and autumn stall (October) for one year. The blood for tests was collected from the jugular vein before morning feeding in winter-stall, summer-pasture, and autumn-stall hold periods. The composition and nutrition of the diets of cows were also analyzed. Changes in the content of 25-OHD3 in the blood of cows were determined depending on the season and conditions of keeping. The lowest level of 25-OHD3 was in the winter-stall holding period and was 22.38 ± 3.58 nmol/l and the highest was in the summer pasture. In the autumn-stall period, the level of 25-OНD3 decreased relative to the summer pasture, but was higher than in the winter-stall (P < 0.05). The total calcium content of the serum of cows was highest in the summer pasture and winter-stallion periods and was, respectively, 2.96 and 2.90 mmol/l. In the autumn-stalling period, the total calcium content decreased and was significantly lower compared to the summer pasture period (P < 0.05). Changes in the total calcium content of cows' blood during different retention periods were due to changes in its fractions. The activity of total alkaline phosphatase and its bone isoenzyme in the blood of cows at 4 months of lactation varied in contrast to the content of 25-OНD3.


Author(s):  
R. Stavetska ◽  
Y. Dynko ◽  
M. Bushtruk ◽  
I. Starostenko ◽  
O. Babenko ◽  
...  

It has been experimentally proven that the strength and direction of the connection between the linear type traits evaluation of the Ukrainian black-and-white dairy breed cows and their body build depends on the type of the build and the sign of the exterior. The study was conducted in 2018 on a breeding plant of the Ukrainian Black-and-White Dairy breed SPC named Shchorsa in Kiev region (n=89). Differentiation of cows on types of their body build was conducted by the method proposed by Chernenko (low-capacity, mid-capacity and high-capacity types) and Kolesnik (by the massiveness index – on the friable and dense, the bone index – delicate and rough, the wide-breast and wide-rump indices – on narrow-body and wide-body types). It was found that the cow-heiferswere well-developed, had intermediate stature and optimum body condition score, deep body, and wide, slight slope rump. They alsohad desirable rear legs, set and foot angle but some toe-out, the udder was well-balanced. Under the good total score of the exterior of studied cows there were some animals with short stature (134– 136 cm), very wide rump, straight rear legs side view and toe-out, extremely steep foot angle, loose fore udder attachment, deep udder below hocks and front teat placement inside of quarter. The degree of development of the studied linear traits of cow-heifers confirms their in-herd variability. The variability of the exterior traits varies from 10,7 % (body depth) to 38,6 % (stature). In addition to the stature of a significant variability was characterized of angularity (22,4 %), rear legs set (22,5 %), body condition score (21,8 %), as well as traits that describe udder: rear udder attachment (31,4 %), udder depth (32,6 %) and front and rear teat position (31,3 % and 25,1 %, respectively). The high variability of linear traits indicates the possibility of effective their selection. As a result of the research, it has been established that the strength and direction of the correlations between the linear type traits evaluation of Ukrainian Black-and-White Dairy breed cows and the type of their body build depends on the type of the body build and the linear trait. The low-, mid- and high-capacity bodies build types (according to Chernenko) reliably correlate with chest width (r=0,58) and body depth (r=0,48), rear legs rear view (r=0,30), the central ligament (r=0,28), udder depth (r =-0,22) and teat length (r=0,25), P˂0,01–0,001. Friable and dense bodies build (according to Kolesnik) were most strongly associated with stature (r=-0,28), chest width (r =-0,70), and body depth (r=-0,38), as well as with rear legs rear view (r=-0,23), P˂0,05...0,001; delicate and rough bodies build – with stature (r=-0,51), angularity (r=-0,22),rump width (r=-0,35), udder depth (r=-0,27) and rear udder attachment (r =0,20), P ˂ 0,05–0,001; narrow-body and wide-body types – with chest width (r=0,30) and rump width (r=0,23), P˂0,05–0,01. Linear type trait evaluation of cows depends on the body build. The strength and direction of the correlations between types of body build and linear traits showed that the most informative is the classification of the body build by low-, mid- and high-capacity according to Chernenko – on the average η2x=17,5 %. The less informative – by narrow-body and wide-body types according to Kolesnik – η2x=7,6 %. The classification of cows on delicate-rough, narrow-body and wide-body types (according to Kolesnik) according to informativeness and reliability can have the intermediate position – η2x=10,0–11,5 %. The strongest influence on the body build has the cows’ height (η2x=27,9 % on average for all studied types of body build), chest width (η2x=33,5 %), body depth (η2x=21,8 %), rump width (η2x=24,1 %) and rear legs (η2x=18,7 %). Key words: cows, linear type trait evaluation, body build, correlation, power of influence.


2017 ◽  
Vol 54 ◽  
pp. 112-119
Author(s):  
L. M. Khmelnychy

The estimation of the first-born cows of Ukrainian Black-and-White dairy breed by the exterior type in the leading breeding herds of Cherkassy region by the method of linear classification (n = 566) has been carried out. The results of the evaluated animals by the 100-score system indicate that within the group of traits, the average level of assessment is "good with the plus". The animals have been differed by the good development of group features characterizing the dairy type (82.4 score), body condition (83.5 score), limbs (82.8 score), udder (82.7 score) and overall score (82.8 score). The degree of development 18 descriptive traits of the exterior of cows, has been predicted by the method of linear classification, indicates their significant intraherd variability. Animals have the moderate height, deep body and angularity. The rump angle, the angle of hock joint and the teats length, according to the variability of scores in the range of 17.8–22.4%, have an average optimal development. The level of scores for the rear width (5.9 score), attachment of the fore (6.3 score) and the rear parts (6.0 score) of the udder testifies to their good development. The body structure of the cows-firstborn has a sufficient description of the type traits that determine their milk yield. At the present stage of selection and breeding work due to the use of bull-sires different origins and breeding value on the local population livestock with significant genotype diversity, the degree of influence each of the hereditary factors in the overall variability of linear features has a scientific and practical significance. In our studies, the force of influence conditional bloodiness of the Holstein mother's breed in the overall fraction of variability of linear type traits within the exterior complexes and the overall assessment takes a significant percentage - from 24.9 to 31.5 with a high degree of reliability according to Fischer's criterion. Among the descriptive traits, the proportion of influence the conditional bloodiness of mother, which is confirmed by high statistical confidence, ranges from 14.4 to 19.8%, and refers to the traits important for breeding. The level of coefficients of influence the father's breeding value in the total phenotypic variability for some of the linear traits is high and reliable. First of all it concerns group traits of the exterior, the development of which is determined by the pedigree value of father by 37.1–41.8%, and the overall assessment of type – by 46.6%. The coefficients of influence father's breeding value in most of the descriptive traits have high reliability (P < 0,001). The coefficients of the force of influence father, depending on the heredity of Holstein for the linear traits of almost the same level as his pedigree value. Since in this case the organized factor is purebreed Holstein and crossbreed bulls-sires of domestic selection, the positive role of Holstein heredity in improving the exterior type of cows has been traced realistically. The necessity of linear breeding, as an effective method in the system of dairy cattle breeding, has been substantiated by reliable values of the coefficients of the force of influence of father's line on the linear traits of offspring (3.4–29.5%). Despite the significant variability of coefficients of the force of influence on the linear traits, most of them have a high level of reliability within the evaluated body parts important for selection. The effect of cows belonging to the maternal line on the development of features of the body structure is insignificant and unreliable (5.3–9.2%). In the process of breeding aimed at improving cows of dairy herd by the exterior type, the pedigree value of parents has been assessed according to the method of linear classification of their daughters and their belonging to perspective genealogical formations.


2020 ◽  
Vol 11 (2) ◽  
pp. 16-27
Author(s):  
L. M. Danets ◽  
I. V. Tkachova ◽  
V. P. Shablia

The duration of productive use is a complex integral feature and is determined by both genetic and paratypical factors. This feature should ensure maximum milk productivity of animals, economic efficiency of dairy farms and generally limit the cost of raising and keeping cows. Research conducted in the experimental farms of state enterprises "Kutuzovka", Kharkov district of Kharkov region using cow’s Ukrainian black-and-white dairy breed (4038 cows). We studied the duration of productive use of cows divided into gradations according to the conditional bloodline for the Holstein breed, depending on the weight in the control age periods of cultivation (at birth, at 6, 12 and 18 months). The maximum value of the duration of productive use was recorded in the group of cows with a share of conditional blood for the Holstein breed up to 30% inclusive (the average duration of productive use is 2.77 lactations). The highest indicator of the duration of productive use of the studied animals was 4.09 lactations. Among cows with a share of conditional blood for the Holstein breed up to 30% longer produced those born with a weight of more than 40 kg (4.09 lactations), at 6 months of age weighed 100-149 kg, at 12 months – 200-249 kg, at 18 months of age – 350-399 kg. Among animals with a share of bloodline of 31-60 %, those that had a weight at the age of 6 months produced the longest: 155-190 kg (3.17 lactations), at 12 months – 250-299 kg (2.98 lactations), at 18 months – 350-399 kg (3.06 lactations). In the most numerous gradation with the share of bloodline for the Holstein breed 61-90 %, the longest productive use was characterized by animals born with a weight of 30-39 kg (2.12 lactation), at 6 months of age they weighed more than 200 kg (3.29 lactation), at 12 months-300-349 kg (3.40 lactation), at 18 months – 400-449 kg (2.82 lactation). The average duration of productive use of cows of this grade is quite low – in the range of 2.82-3.29 lactations. The highest degree of influence on the duration of productive use in this gradation of animals was recorded by the live weight factor at 6 months of age (η2 = 10.8). Сcomparative assessment of the cows gradation with a share of conditional blood for the Holstein breed of 91 % or more found that the longest productive use was characterized by cows that had a live weight at birth of 30-39 kg (1.93 lactation), in 6 months – 155-199 kg (2.73 lactation), in 12 months – 250-299 kg (2.87 lactation) and in 18 months – 350-399 kg (2.62 lactation). The degree of influence of live weight at this age on the duration of productive use of cows is insignificant, except for live weight at 6 months of age (η2 = 11.8).


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