Chemical Characterization and in Vitro Antitumor Activity of the Essential Oils from the Leaves and Flowers of Callistemon viminalis

Pelargonium graveolens (Geranium) is a source of the finest quality of fragrance and its essential oils are used as antibacterial, and antifungal agents. The aim of the current research is to determine chemical constituents in the essential oil of P. graveolens by GC-MS and evaluate its antidiabetic activity via α-glucosidase inhibition assay. The chemical composition of P. graveolens essential oil was determined by GC/MS and its antidiabetic activity was assessed through inhibition of α-glucosidase enzyme in in-vitro models. GC-MS analysis determines 36 chemical components in the essential oil of P. graveolens leaves, and citronellyl isovalerate (10.41 %), menthol (9.61 %), linalool (8.63 %), p-menthone (6.31 %), and geranyl tiglate (4.99 %) were recorded as major constituents. The essential oil of P. graveolens leaves showed concentration dependant inhibition of α-glucosidase enzyme ranging from 28.13±1.41 to 74.24±2.53 µg/mL for concentration ranging from 31.25 to 1000 µg/mL. The IC50 values for of P. graveolens and acarbose were found as 93.72±4.76 and 80.4±2.17 µg/mL, respectively against the α-glucosidase enzyme. The study finding explores the chemical components of P. graveolens growing in the Iraqi Kurdistan region and scientifically supported its possible use in diabetic patients for controlling postprandial hyperglycemia.

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Chemical characterization and determination of in vivo and in vitro antifungal activity of essential oils from four Eucalyptus species against the Hemileia vastatrix Berk and Br fungus, the agent of coffee leaf rust

Australian Journal of Crop Science ◽

10.21475/ajcs.20.14.09.p2249 ◽

2020 ◽

pp. 1379-1384

Author(s):

Alex Rodrigues Silva Caetano ◽

Sara Maria Chalfoun ◽

Mario Lúcio Vilela Resende ◽

Caroline Lima Angélico ◽

Wilder Douglas Santiago ◽

...

Keyword(s):

Gas Chromatography ◽

Antifungal Activity ◽

Essential Oil ◽

Essential Oils ◽

Chemical Characterization ◽

Eucalyptus Species ◽

Hemileia Vastatrix ◽

Antifungal Activities

Essential oils, also known as volatile oils, are substances produced through the secondary metabolism of plants. In this study, we determined the chemical composition and the in vitro and in vivo antifungal activity of the essential oils from four species of Eucalyptus, Eucalyptus citriodora, Eucalyptus camaldulensis, Eucalyptus grandis and Eucalyptus microcorys, against the Hemileia vastatrix fungus. The essential oils from these four species of Eucalyptus were extracted from their leaves by the hydrodistillation technique using a modified Clevenger apparatus. The chemical characterization was performed by gas chromatography coupled with a mass spectrometer detector and by gas chromatography using a flame ionization detector. The antifungal activities of the essential oils against H. vastatrix were studied by evaluating the percentage of spore germination using the microdilution test for in vitro assays. The curative and preventive effects were evaluated in in vivo tests. The principal constituents of the essential oil from E. citriodora were citronellal, citronellol and isopulegol, while E. camaldulensis produced 1,8-cineole, α-terpineol and α-pinene. 1,8-cineole, α-pinene and α-terpineol were obtained from E. grandis and 1,8-cineole, α-pinene and trans-pinocarveol were the principal components in the essential oil of E. microcorys. In vitro and in vivo antifungal activities against the fungus under study were observed for most of the essential oils, except the essential oil from E. microcorys, for which no preventive antifungal activity was observed. Only the curing of infection by the H. vastatrix fungus was observed with this oil.

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Solid Polymeric Nanoparticles of Albendazole: Synthesis, Physico-Chemical Characterization and Biological Activity

Molecules ◽

10.3390/molecules25215130 ◽

2020 ◽

Vol 25 (21) ◽

pp. 5130 ◽

Cited By ~ 1

Author(s):

Roxana Racoviceanu ◽

Cristina Trandafirescu ◽

Mirela Voicu ◽

Roxana Ghiulai ◽

Florin Borcan ◽

...

Keyword(s):

Breast Cancer ◽

Antitumor Activity ◽

Benzimidazole Derivative ◽

Chemical Characterization ◽

Breast Cancer Cell Lines ◽

Dependent Manner ◽

Concentration Dependent Manner ◽

Encapsulation Process ◽

Mcf 7

Albendazole is a benzimidazole derivative with documented antitumor activity and low toxicity to healthy cells. The major disadvantage in terms of clinical use is its low aqueous solubility which limits its bioavailability. Albendazole was incorporated into stable and homogeneous polyurethane structures with the aim of obtaining an improved drug delivery system model. Spectral and thermal analysis was used to investigate the encapsulation process and confirmed the presence of albendazole inside the nanoparticles. The in vitro anticancer properties of albendazole encapsulated in polyurethane structures versus the un-encapsulated compound were tested on two breast cancer cell lines, MCF-7 and MDA-MB-231, in terms of cellular viability and apoptosis induction. The study showed that the encapsulation process enhanced the antitumor activity of albendazole on the MCF-7 and MDA-MB-23 breast cancer lines. The cytotoxic activity manifested in a concentration-dependent manner and was accompanied by changes in cell morphology and nuclear fragmentation.

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Comparative Chemical, Cytotoxicity and Antileishmanial Properties of Essential Oils from Chenopodium ambrosioides

Natural Product Communications ◽

10.1177/1934578x1100600232 ◽

2011 ◽

Vol 6 (2) ◽

pp. 1934578X1100600 ◽

Cited By ~ 8

Author(s):

Lianet Monzote ◽

Marcelina R. Nance ◽

Marley García ◽

Ramón Scull ◽

William N. Setzer

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Chemical Characterization ◽

In Vitro Cytotoxicity ◽

Chenopodium Ambrosioides ◽

Popular Medicine ◽

Lead Compounds ◽

Antileishmanial Activities ◽

Treatment Alternatives

In countries where leishmaniasis is endemic, there are not very many treatment alternatives and most options have problems associated with their use. Plants and their natural products constitute good sources of interesting lead compounds that could be potentially active against Leishmania. Chenopodium ambrosioides is a plant that is widely used in popular medicine and its antiparasitic effects have been documented, including the antileishmanial potentialities of Chenopodium oil. The objective of this study was to determine the chemical composition, in-vitro cytotoxicity and antileishmanial activity of essential oils extracted from C. ambrosioides, which received different treatments prior to extraction. The chemical characterization by GC-MS of the three essential oil samples showed similar composition and the major components were α-terpinene (17.0-20.7%), p-cymene (20.2-21.1%) and ascaridole (30.5-47.1%). The essential oils exhibited similar antileishmanial activities against intracellular amastigote form, with IC50 values between 4.7 and 12.4 μg/mL. However, a lower cytotoxicity was displayed by the essential oil extracted from fresh green vegetable material, which was statistically different ( P < 0.05) from the other samples. This study demonstrated that the prior treatment of plant material did not interfere with the antiparasitic activity of essential oils from C. ambrosioides but did change their cytotoxicity, which should be taken into account in further studies.

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Chemical composition, acetylcholine esterase and Anti-bacterial activities of essential oils of Elaeosilenum thapsioides (Desf.) Maire from Algeria

Current Topics in Medicinal Chemistry ◽

10.2174/1568026621666210813110847 ◽

2021 ◽

Vol 21 ◽

Author(s):

Zakaria Barbeche ◽

Hocine Laouer ◽

Ramazan Erenler ◽

Mohamed Hajji ◽

Guido Flamini ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Inhibitory Activity ◽

Research Work ◽

Chemical Characterization ◽

Diffusion Method ◽

Aerial Parts ◽

Ache Inhibitory Activity

Background: Traditionally, The Algerian medicinal plant Elaeosilenum thapsioides have been used for many diseases. Objective: The present research work is aimed to explore the chemical and biological characterization of essential oil of, Elaeoselinum thapsioides (Desf.) Maire. Methods : The essential oils were obtained by hydrodistillation of different Elaeosilenum thapsioides (Apiaceae) aerial parts samples collected from two different regions (Mahouane and Megres) Setif, Eastern Algeria. The chemical characterization of the obtained essential oils was investigated in the present work for the first time by GC and GC-MS. Besides, they were evaluated for their in-vitro acetylcholinesterase (AChE) inhibitory activity whose enzyme hyperactivity is involved in Alzheimer’s disease. Using Ellman’s spectrophotometric method, additionally, their in-vitro antimicrobial activity was assessed by the disc diffusion method. Both activities were performed at various oil concentrations. Results: The GC/MS analysis of the aerial parts (leaves, stems, flowers, and seeds) essential oils of E. thapsioides revealed the presence of dominant compounds and others in small quantities, identifying 47 chemical molecules. Monoterpene hydrocarbons were the main components, ranging from 72.78 % to 99.13 %. Oxygenated monoterpenes and oxygenated sesquiterpenes ranged between (1.37 % -17.25 %) and (0.12 % -3.53 %) in leaves and stem essential oils. Sesquiterpene hydrocarbons were present in small to large quantities in the essential oils of both populations, with contents ranging from 0.69 % to 13.44 %. For the Isothiocyanates, their presence was recorded in leaves and stem essential oils from Mahouane and Merges with 9.73 % and 3.72 %, consecutively. Indeed, the essential oil of the Mahouane stem showed the highest AChE inhibitory activity among all the tested essential oils. Whereas the highest antibacterial activity was shown by the essential oil obtained from Megres leaves against Bacillus cereus ATCC 11778. Conclusion: The oils exhibited a moderate inhibitory activity in both activities.

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Characterization and Phytotoxicity Assessment of Essential Oils from Plant Byproducts

Molecules ◽

10.3390/molecules24162941 ◽

2019 ◽

Vol 24 (16) ◽

pp. 2941 ◽

Cited By ~ 8

Author(s):

Antonella Smeriglio ◽

Domenico Trombetta ◽

Laura Cornara ◽

Marco Valussi ◽

Vincenzo De Feo ◽

...

Keyword(s):

Essential Oils ◽

Cannabis Sativa ◽

Biological Activities ◽

Lolium Multiflorum ◽

Chemical Characterization ◽

Zingiber Officinale ◽

Lepidium Sativum ◽

Cupressus Sempervirens ◽

Wide Range

The present work describes the chemical characterization and the phytotoxicity assessment of essential oils (EOs) obtained from spent materials or pruning waste of four plant species: Zingiber officinale Roscoe used in the juicing industry, Pistacia vera L. var. Bronte used in the food industry, discarded material of industrial hemp (Cannabis sativa L. var. Futura 75), and pruning waste from Cupressus sempervirens L. The phytochemical profile of the EOs was evaluated by gas chromatographic flame ionization detection (GC-FID) and GC-MS analyses, which highlighted the presence of several compounds with a wide range of biological activities. Among them, application possibilities in agriculture were evaluated by studying the phytotoxic activity in vitro against germination and initial radical growth of several seeds such as Raphanus sativus L., Lepidium sativum L., Lactuca sativa L., Solanum lycopersicum L., Lolium multiflorum Lam., and Portulaca oleracea L.

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Ethnopharmacology, Phytochemistry, and Pharmacological Properties of Thymus satureioides Coss.

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2021/6673838 ◽

2021 ◽

Vol 2021 ◽

pp. 1-23

Author(s):

Naoufal El Hachlafi ◽

Abderrahim Chebat ◽

Kawtar Fikri-Benbrahim

Keyword(s):

Essential Oils ◽

Bioactive Compounds ◽

Biological Activities ◽

Chemical Characterization ◽

Pharmacokinetic Studies ◽

Pharmacological Studies ◽

Thymus Satureioides

Thymus satureioides Coss. (Lamiaceae) is a Moroccan medicinal plant locally known as “Azkouni” or “Zaitra.” It is widely used in traditional medicine to treat various ailments, including hypertension, diabetes, cold, fever, dermatological and circulatory disorders, immune problems, bronchitis, nociception, cooling, pharyngitis, cough, and influenza. The current review aims to critically summarize the literature on ethnopharmacological uses, chemical profile, and pharmacological investigations of T. satureioides in order to provide data support and scientific evidences for further investigations. Electronic databases such as Scopus, PubMed, Web of Science, SciFinder, ScienceDirect, Google Scholar, and Medline were used to gather data on T. satureioides. Chemical characterization of T. satureioides essential oils (EOs) and extracts allowed to identify a total of 139 bioactive compounds, mainly belonging to the terpenoids, phenolic acids, and flavonoids classes. T. satureioides especially its essential oils exhibited numerous biological activities such as antibacterial, antifungal, anti-inflammatory, antioxidant, antidiabetic, anticancer, antiparasitic, and hypolipedemic activities. In light of these findings, further studies to transmute the traditional application of T. satureioides into scientific-based information are strongly required. Additional in vivo pharmacological studies are recommended to validate the results of the in vitro studies. Moreover, comprehensive preclinical and clinical trials on the pharmacological mechanisms of action of this plant and its bioactive compounds on molecular targets should be performed. Finally, more efforts must be focused on toxicological assessments and pharmacokinetic studies, in order to ensure the safety and the efficiency of T. satureioides.

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Essential Oils from the Leaves and Flowers of <i>Callistemon viminalis</i>: Chemical Characterization and Evaluation of the Insecticide and Antifungal Activities

American Journal of Plant Sciences ◽

10.4236/ajps.2017.810171 ◽

2017 ◽

Vol 08 (10) ◽

pp. 2516-2529

Author(s):

Thais Aparecida Sales ◽

Maria Das Graças Cardoso ◽

Luiz Gustavo De Lima Guimarães ◽

Karen Caroline Camargo ◽

Danúbia.A.C.S Rezende ◽

...

Keyword(s):

Essential Oils ◽

Chemical Characterization ◽

Antifungal Activities ◽

Callistemon Viminalis

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Chemical characterization and in vitro antitumor activity of a single-component polysaccharide from Taxus chinensis var. mairei

Carbohydrate Polymers ◽

10.1016/j.carbpol.2015.06.107 ◽

2015 ◽

Vol 133 ◽

pp. 294-301 ◽

Cited By ~ 19

Author(s):

Mianbin Wu ◽

Feifei Zhang ◽

Zhangping Yu ◽

Jianping Lin ◽

Lirong Yang

Keyword(s):

Antitumor Activity ◽

Chemical Characterization ◽

Single Component ◽

Taxus Chinensis

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Chemical characterization of the Allium sativum and Origanum vulgare essential oils and their inhibition effect on the growth of some food pathogens

Revista Brasileira de Plantas Medicinais ◽

10.1590/1983-084x/10_120 ◽

2014 ◽

Vol 16 (4) ◽

pp. 804-811 ◽

Cited By ~ 7

Author(s):

A.C.T. Mallet ◽

M.G Cardoso ◽

P.E. Souza ◽

S.M.F. Machado ◽

M.A. Andrade ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Allium Sativum ◽

Chemical Characterization ◽

In Vitro Tests ◽

Diallyl Disulfide ◽

Food Contaminants ◽

Origanum Vulgare ◽

Oregano Essential Oil

This study sought to evaluate the chemical composition of the Allium sativum and Origanum vulgare essential oils and their effect on the growth inhibition of microorganisms, such as P. aeruginosa, S. Choleraesuis, A. flavus, A. niger and P. simplicissimum, important food contaminants. The main constituents of the oregano essential oil were 4-terpineol (27.03%), γ-terpinene (20.04%), and β-cymene (6.34%), and the main constituents of the garlic essential oil were diallyl trisulfide (38, 81%), diallyl disulfide (25.23%), and methyl allyl trisulfide (12.52%). Inhibition zones were formed in in vitro tests on the bacteria S. Choleraesuis and P. aeruginosa, except for A. sativum against P. aeruginosa. The inhibition of mycelial growth caused by the oregano essential oil occurred with the concentrations of 0.10, 0.03 and 0.05 mg mL-1 for the A. flavus, A. niger and P. simplicissimum fungi, respectively. The CMI for the garlic oil began at the 0.03 mg mL-1 concentration for all species of fungi. The oils presented an inhibitory effect against the microorganisms studied and constitute an alternative for microbiological control in food.

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