Evaluation of The Antioxidant Activity of Root Extract of Pepper Fruit (<i>Dennetia Tripetala</i>), and it’s Potential for the Inhibition of Lipid Peroxidation

Plants have been used for medical purposes since ancient times. However, a detailed analysis of their biological properties and their associated active compounds is needed to justify their therapeutic use in modern medicine. The aim of the study was to identify and quantify the phenolics present in hydromethanolic extracts of the roots and shoots of the Chinese Salvia species, Salvia bulleyana. The qualitative and quantitative analyses were carried out by ultrahigh-performance liquid chromatography with electrospray ionization mass spectrometry detection (UHPLC-PDA-ESI-MS), and high-performance liquid chromatography with photodiode array (HPLC-PDA) detection. The extracts of S. bulleyana were also screened for their antioxidant activity using ferric ion (Fe3+) reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) cation (ABTS), superoxide radical anion (O2•–), and inhibition of lipid peroxidation assays. The S. bulleyana extracts were found to contain 38 substances, of which 36 were phenols, with a total level of 14.4 mg/g DW (dry weight) in shoots, and 23.1 mg/g DW in roots. Twenty-eight phenols were polyphenolic acids or their derivatives, the most abundant in shoots being rosmarinic acid, and in roots, salvianolic acid K followed by rosmarinic acid. The other major phenolic acids were caffeic acid, caffeoyl-threonic acids, isomers of lithospermic acid, salvianolic acid F, salvianolic acid B, and yunnaneic acid E. In addition to polyphenolic acids, nine flavonoids were detected in the shoot extract. While both extracts showed significant antioxidant activity, the shoot extract, containing both polyphenolic acids and flavonoids, demonstrated a slightly greater antioxidant potential in some of the anti-radical tests than the roots. However, the root extract proved to be slightly more effective in the lipid peroxidation inhibition test. Thus, S. bulleyana was demonstrated as a promising source of antioxidants, and worthy of further more detailed studies.

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Chemical characterization and antioxidant activity of Eryngium campestre L., Apiaceae from Kosovo

Macedonian Pharmaceutical Bulletin ◽

10.33320/maced.pharm.bull.2009.55.002 ◽

2009 ◽

Vol 55 ◽

pp. 22-32 ◽

Cited By ~ 8

Author(s):

Flurim Nebija ◽

Gjoshe Stefkov ◽

Marija Karapandzova ◽

Trajche Stafilov ◽

Tatjana Kadifkova Panovska ◽

...

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Activity ◽

Chemical Composition ◽

Hydroxyl Radical ◽

Aerial Part ◽

Radical Scavenging ◽

Liver Homogenate ◽

Ethanol Extract ◽

Root Extract

This study is outlined to define the chemical composition and in vitro antioxidant activity of the extracts of aerial part and root of Eryngium campestre L. (Apiaceae) from Kosovo. Analysis of the chemical composition include determination of total ash, ash insoluble in hydrochloric acid, loss on drying and the content of water extract, as well as determination of flavonoids in aerial part and hemolytic activity of the root. The mineral composition (Zn, Fe, Cu, Mn, Ni, K, Co, Pb, Cd and Cr) in aerial parts and root has been studied using atomic absorption spectroscopy (AAS and ETAAS). Different part of E. campestre accumulate different amounts of investigated minerals. Antioxidant activity was determined by four various testing systems: DPPH assay, inhibition of production of hydroxyl radical, β-carotenebleaching assay, and inhibition of lipid peroxidation (TBA test). In DPPH system, ethanol extract of root of E. campestre exhibited higher radical-scavenging activity (IC50 = 0.72 mg ml-1) compared to the extract of the aerial part (IC50 = 1.14 mg ml-1). On the other hand, aerial part ethanol extract has exhibited stronger inhibition capacity on the production of hydroxyl radical in deoxyribose system than the root extract (50% and 45%, respectively). However, both ethanol extracts of E. campestre exhibited low antioxidant activity in β-carotenebleaching assay as well as, low capacity for inhibition of spontaneous lipid peroxidation in rat liver homogenate

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Antioxidant activity of the root extract of Combretum dolichopetalum and the isolated constituents

Planta Medica ◽

10.1055/s-0035-1565434 ◽

2015 ◽

Vol 81 (16) ◽

Author(s):

PF Uzor ◽

PO Osadebe ◽

Z Liu ◽

W Ebrahim ◽

P Proksch

Keyword(s):

Antioxidant Activity ◽

Root Extract

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Assessment of antioxidant and antibacterial activities of crude extracts of verbena officinalis Linn root or Atuch (Amharic)

10.31221/osf.io/pzh3s ◽

2019 ◽

Cited By ~ 1

Author(s):

Chem Int

Keyword(s):

Antioxidant Activity ◽

Methanolic Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Dry Weight ◽

Antimicrobial Activities ◽

Total Flavonoid ◽

Root Extract ◽

Antioxidant Power ◽

Crude Extracts

Verbena officinalis Linn is a traditionally known medicinal plant which is used against a number of diseases including inflammatory conditions. In this study its antioxidant activity (reducing powers, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities), ferric reduction activity potential (FRAP), total flavonoid concentration and antimicrobial activities of 80%, 90%, 100% methanol and chloroform extracts of V. officinalis Linn root and 90% and100% methanol leaf extracts were determined. Its antioxidant activity increases with increase in amount of extract (10% to 40%v/v). Total flavonoid content (TFC) varied from 73.32±0.002 mgQE/100g of dry weight (90% methanol) to 42.39±0.032 mgQE/100g dry weight (chloroform), 2,2-diphenyl-1-picrylhydrazyl (DPPH), radical scavenging activity (%) was varied between 87.39% (90% methanol) to 45.57% (chloroform) while Ferric reducing antioxidant power was observed between 372.93±0.04 mgAAE/100 g extract (90% methanol) to 129.41±0.026 mgAAE/100 g chloroform in the root extract. The methanolic extract of the leaf showed less antioxidant activity than the methanolic extract of the root. Crude extracts of V. officinalis root showed various degree of antimicrobial activity towards drug resistance microbial pathogens. Growth inhibition tests against bacterial pathogens demonstrated concentration dependence. Moreover, gram positive bacteria were more susceptible to V. officinalis root extract when compared to gram negative bacteria. In general V. officinalis root and leave extracts possess strong antioxidant and antimicrobial activities.

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In Vitro Antioxidant Activity of Litsea martabanica Root Extract and Its Hepatoprotective Effect on Chlorpyrifos-Induced Toxicity in Rats

Molecules ◽

10.3390/molecules26071906 ◽

2021 ◽

Vol 26 (7) ◽

pp. 1906

Author(s):

Phraepakaporn Kunnaja ◽

Sunee Chansakaow ◽

Absorn Wittayapraparat ◽

Pedcharada Yusuk ◽

Seewaboon Sireeratawong

Keyword(s):

Antioxidant Activity ◽

Antioxidant Properties ◽

Water Extract ◽

Acetylcholinesterase Activity ◽

Liver Cells ◽

Scientific Data ◽

Total Phenolic ◽

Root Extract ◽

Antioxidant Power ◽

Detoxifying Agent

In Thailand, people in the highland communities whose occupational exposure to pesticides used the root of Litsea martabanica as a detoxifying agent. However, the scientific data to support the traditional use of this plant are insufficient. This study aimed to evaluate the antioxidant activity and anti-pesticide potential of L. martabanica root extract. Antioxidant properties were investigated by 2,2′-diphenyl-1-picrylhydrazyl (DPPH) assay, superoxide radicals scavenging assay, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay, ferric reducing antioxidant power (FRAP), and total phenolic content determination. In all assays, L. martabanica extracts and their fractions exhibited high antioxidant activities differently. The water extract is traditionally used as a detoxifying agent. Therefore, it was chosen for in vivo experiments. The rats received the extract in a way that mimics the traditional methods of tribal communities followed by chlorpyrifos for 16 days. The results showed that acetylcholinesterase activity decreases in pesticide-exposed rats. Treatment with the extract caused increasing acetylcholinesterase activity in the rats. Therefore, L. martabanica extract may potentially be used as a detoxifying agent, especially for the chlorpyrifos pesticide. The antioxidant properties of L. martabanica may provide a beneficial effect by protecting liver cells from damage caused by free radicals. Histopathology results revealed no liver cell necrosis and showed the regeneration of liver cells in the treatment group. L. martabanica extract did not cause changes in behavior, liver weight, hematological and biochemical profiles of the rats.

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Optimising Conditions for Encapsulation of Salacia chinensis Root Extract enriched with Phenolic Compounds

Current Nutraceuticals ◽

10.2174/2665978602666210805094012 ◽

2021 ◽

Vol 02 ◽

Author(s):

Thanh V. Ngo ◽

Christopher J. Scarlett ◽

Michael C. Bowyer ◽

Rebecca McCullum ◽

Quan V. Vuong

Keyword(s):

Antioxidant Activity ◽

Spray Drying ◽

Feed Rate ◽

Freeze Drying ◽

Antioxidant Activities ◽

Inlet Temperature ◽

Root Extract ◽

Optimal Conditions ◽

Dried Extract ◽

Drying Conditions

Background: S. chinensis extract contains bioactive compounds, which exhibit high antioxidant activities. However, for commercial uses, it is necessary to encapsulate the extract to protect it from degradation. Objective: This study aimed to optimise spray-drying conditions and then compare with freeze-drying to identify the most suitable conditions for encapsulation of Salacia chinensis L. root extract. Method: Three factors of spray-drying encapsulation, including maltodextrin concentration, inlet temperature and feed rate, have been tested for the impacts on the physical and phytochemical properties of S. chinensis root extract. Based on the optimal conditions, the spray-drying was then compared with freeze-drying. Results: The results showed that maltodextrin concentration, inlet temperature and feed rate had significant impacts on recovery yield, phenolics, mangiferin and antioxidant activity of the spray-dried extract. The optimal spray-drying encapsulation conditions were maltodextrin concentration of 20 %, inlet temperature of 130ºC and feed rate of 9 mL/min. Under these optimal conditions, the encapsulated extract had comparable solubility, total phenolics, mangiferin, and antioxidant activity, lower bulk density, moisture content, and water activity as compared to encapsulated extract made using the freeze-drying technique. These optimal spray-drying conditions are recommended to encapsulate the extract of S. chinensis root. Conclusion: Spray-drying was found to be more effective for encapsulation of S. chinensis root extract than freeze-drying. Therefore, spray-drying is recommended for further applications.

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Antioxidant activity of rice plants sprayed with herbicides

Pesquisa Agropecuária Tropical ◽

10.1590/1983-40632016v4638011 ◽

2016 ◽

Vol 46 (1) ◽

pp. 28-34 ◽

Cited By ~ 5

Author(s):

Marcos André Nohatto ◽

Dirceu Agostinetto ◽

Ana Claudia Langaro ◽

Claudia de Oliveira ◽

Queli Ruchel

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Antioxidant Activity ◽

Acetolactate Synthase ◽

Defense Behavior ◽

Sod Activity ◽

Rice Plants ◽

Synthase Inhibitor ◽

Acetyl Coenzyme A Carboxylase ◽

Lower Capacity

ABSTRACT Understanding the physiological defense behavior of plants subjected to herbicide application may help to identify products with higher or lower capacity to cause oxidative stress in crops. This study aimed at evaluating the effect of herbicides in the antioxidant activity of rice plants. The experimental design was completely randomized, with six replications. Treatments consisted of the herbicides bentazon (photosystem II inhibitor; 960 g ha-1), penoxsulam (acetolactate synthase inhibitor; 60 g ha-1), cyhalofop-butyl (acetyl coenzyme-A carboxylase inhibitor; 315 g ha-1) and a control. After the herbicides application, samples of rice shoots were collected at 12, 24, 48 and 96 hours after application (HAA). The components evaluated were hydrogen peroxide (H2O2), lipid peroxidation and activity of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). Bentazon (up to 24 HAA) and penoxsulam (48 and 96 HAA) reduced the CAT activity. Moreover, these herbicides increased the levels of H2O2, lipid peroxidation and SOD activity, indicating a condition of oxidative stress in rice plants. The cyhalofop-butyl herbicide did not alter the antioxidant activity, showing that it causes less stress to the crop.

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Antioxidant Activities of the Aqueous Extracts of Pedalium murex D. Royen EX L. Fruit and Leafy Stem

European Journal of Medicinal Plants ◽

10.9734/ejmp/2021/v32i830406 ◽

2021 ◽

pp. 1-9

Author(s):

Gerard Bessan Dossou-Agoin ◽

Habib Ganfon ◽

Fidèle Assogba ◽

Adam Gbankoto ◽

Joachim Gbenou ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Lipid Peroxidation ◽

Antioxidant Activity ◽

Reducing Power ◽

Total Phenolic ◽

Scavenging Activity ◽

Aqueous Extracts ◽

Fruit Extract ◽

Stem Extract ◽

Dpph Scavenging

Aims: The leafy stem and fruit of P.murex have been reported to be used in folk medicine to treat male reproductive system ailments. This study was undertaken to assess the antioxidant potential of the aqueous extracts of P.murex leafy stem and fruit. Methodology: Extracts were prepared by macerating the powder in water. Total phenolics amount were determined by Folin-Ciocalteu reagent, flavonoids were quantified by aluminum chloride method and total tannin content was estimated by hexacyanoferric method. The in vitro antioxidant activity of the extracts were assessed through 2,2´-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay, reducing power assay, hydrogen peroxide (H2O2) inhibition assay and lipid peroxidation assay. Results: Total phenolic compounds, flavonoids and tannins content were respectively equal to 48.91± 1.67 µg/mg Gallic Acid Equivalents (GAE); 56.01± 0.90 µg/mg Rutin Equivalents (RUE); 16.92± 1.22 µg/mg Tannic Acid Equivalents (TAE) for the leafy stem extract while they were equal to 26.26± 0.54 µg/mg GAE; 47.88± 2.39 µg/mg RUE; 7.94± 0.31 µg/mg TAE for the fruit. The leafy stem extract exhibited a more pronounced DPPH scavenging activity, reducing power, hydrogen peroxide scavenging activity and lipid peroxidation activity than the fruit extract. Conclusion: The antioxidant activity of the leafy stem aqueous extract was significantly more potent than that of the fruit extract. Further studies will find out the extracts pharmacological efficacy and innocuity.

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Toxic Effects of Methotrexate on Rat Kidney Recovered by Crocin as a Consequence of Antioxidant Activity and Lipid Peroxidation Prevention

Iranian Biomedical Journal ◽

10.29252/ibj.24.1.39 ◽

2020 ◽

Vol 24 (1) ◽

pp. 39-46

Author(s):

Cyrus Jalili ◽

Ali Ghanbari ◽

Shiva Roshankhah ◽

Mohammad Reza Salahshoor ◽

◽

...

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Activity ◽

Rat Kidney ◽

Toxic Effects

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The effect of bee venom and hyperthermia on the lipoperoxidation and antioxidant activity of the blood of tumor-bearing animals

JOURNAL of SIBERIAN MEDICAL SCIENCES ◽

10.31549/2542-1174-2021-3-25-33 ◽

2021 ◽

pp. 25-33

Author(s):

M.A. Shabalin ◽

◽

A.V. Deryugina ◽

V.V. Nazarova ◽

E.A. Gracheva ◽

...

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Activity ◽

Malignant Tumors ◽

Combined Treatment ◽

Bee Venom ◽

Control Group ◽

Sod Activity ◽

Tumor Bearing ◽

Prolonged Effect ◽

Control Tumor

Introduction. Nowadays, it has been shown that one of the possible ways to increase the effectiveness of the treatment of malignant tumors is the use of combined treatment methods. Aim. To study the intensity of lipid peroxidation (LPO) and the activity of superoxide dismutase (SOD) in hyperthermia against the background of bee venom in the blood of tumor-bearing rats. Materials and methods. The experimental animals were divided into 5 groups: 1st — intact; 2nd — control (tumor-bearing animals (PS-1 tumor strain) with intraperitoneal (IP) administration of saline solution); 3rd, 4th and 5th groups — tumor-bearing animals with IP administration of 0.5 ml of bee venom against the background of hyperthermia 42, 43 and 44°C respectively. The content of diene and triene conjugates, Schiff bases (SB) and SOD activity in the blood of animals was determined. Results. From the 1st day after the end of the experiment, a statistically significant decrease in triene conjugates was recorded in all experimental groups compared to the control group. SB decreased on the 1st–7th day after the end of the experiment with the action of hyperthermia 42°C and bee venom, on the 7th–28th day — with the action of hyperthermia 43°C and bee venom, and on the 28th day — with the action of bee venom and hyperthermia 44°C, which was accompanied by an increase in SOD activity from the 7th day in all experimental groups compared to the control group. Conclusion. Hyperthermia in combination with the action of bee venom causes a decrease in lipid peroxidation products and an increase in antioxidant activity in the blood of tumor-bearing rats. The most effective action, in our opinion, is the use of bee venom against the background of hyperthermia of 43°C, at which a prolonged effect is recorded both with respect to a decrease in the concentration of SB and an increase in the activity of SOD.

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