scholarly journals Total lipid accumulation and fatty acid profiles of microalga Spirulina under different nitrogen and phosphorus concentrations

2014 ◽  
Vol 16 (1) ◽  
pp. 57 ◽  
Author(s):  
RN Bhakar ◽  
B Brahmdutt ◽  
S Pabbi
2007 ◽  
Vol 2 (2) ◽  
pp. 75
Author(s):  
Gede Suwarthama Sumiarsa ◽  
Ronald P. Phelps

<p>Lipid and fatty acid profiles were described for copepod nauplii Apocy clops panamensis from fertilized brackish water ponds, and after being acclimated to fullsea water salinity. Mean total lipid content of copepod nauplii collected from ponds fertilized with inorganic fertilizer combined either with alfalfa meal, rice bran, wheat bran, and a combination of these fertilizers ranged from 5.66 ± 0.15 to 7.76% ± 0.27%. Non-polar (neutral) lipid fraction of pond copepod nauplii was a significantly higher percentage of the total lipid content (74.5 ± 1.8 - 93.5% ± 1.0%) compared to those of polar lipid (6.5 ± 1.0 - 21.3% ± 1.8%) (P= 0.000). DHA/EPA ratio in neutral lipids ranged from 1.8 ± 0.2 - 2.0 ± 0.1 with no significant differences in three fertilization regimes. DHA was 27.5% ± 0.56% of the neutral lipids and EPA 14.8% ± 0.8%. Acclimation of copepod nauplii for six hours from brackish to full-sea water salinity reduced their lipid content and individual dry weight significantly. Mean total lipid content was reduced 44.2%, non-polar lipid was reduced 46.9% and polar lipid was reduced 24.4%. Acclimation altered the DHA/EPA ratio, in the neutral fraction the ratio increased 26.3% but in the polar fraction it decreased 25%.</p>


2010 ◽  
Vol 37 (1) ◽  
pp. 105-112 ◽  
Author(s):  
Bruce Davidson ◽  
Jonathan Sidell ◽  
Jeffrey Rhodes ◽  
Geremy Cliff

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Ozlem Cakmak ◽  
Aynur Altuntas ◽  
Veli Ugurcu ◽  
Hacı Kemal Erdemli ◽  
Sumeyya Akyol

The proximate composition of male and femaleLeuciscus lepidusin Beyşehir Lake was investigated. The fatty acid profiles of total lipid, phospholipid, and triacylglycerol in muscle and liver of male and femaleL. lepiduswere evaluated by gas chromatography. Proximate analyses showed that meat of male and femaleL. lepidushad 15.13 ± 0.04 and 18.75 ± 0.11% fat, 20.42 ± 0.45 and 22.21 ± 0.56% protein, 65.47 ± 1.37 and 61.28 ± 1.03% moisture, and 1.51 ± 0.05 and 1.50 ± 0.03% ash, respectively. The percentage of total saturated fatty acids was higher in liver than in muscle, whereas the total polyunsaturated fatty acid (PUFA) content was the lowest in all fatty acid profiles. The phospholipids contained more PUFAs than triacylglycerol. Analysis of variance indicated significant differences (P<0.05) between male (47.51%) and female (49.98%) muscle PUFAs in total lipid. The proportion of omega 3 (ω3) to omega 6 (ω6) fatty acids of total lipid was 3.15 in male and 3.68 in female. The ratio is an important indicator for comparing the value of fish oil. Therefore, it was concluded thatL. lepiduswas considered to be a high quality product for healthy food choice. Additionally, femaleL. lepidusmay especially be used to produce fish oil supplements from freshwater fish combined with vegetable oils.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yevhen Maltsev ◽  
Zinaida Krivova ◽  
Svetlana Maltseva ◽  
Kateryna Maltseva ◽  
Elena Gorshkova ◽  
...  

AbstractA novel freshwater strain of Coelastrella multistriata MZ–Ch23 was discovered in Tula region, Russia. The identification is based on morphological features, phylogenetic analysis of SSU rDNA gene and ITS1–5.8S rDNA–ITS2 region and predicted secondary structure of the ITS2. Phylogenetic analysis places the novel strain in the “core” Coelastrella clade within the Chlorophyceae. This is the first record of Coelastrella multistriata in the algal flora of Russia. Cultivation experiments were carried out to evaluate growth dynamics of the newly identified strain and the impact of nitrogen and/or phosphorus depletion on the fatty acid profiles and lipid productivity. On the fully supplemented Bold’s basal medium and under phosphorus-depleted conditions as well, the fatty acid profiles were dominated by α-linolenic acid (29.4–38.1% of total fatty acids). Depletion of either nitrogen or both nitrogen and phosphorus was associated with increased content of oleic acid (32.9–33.7%) and linoleic acid (11.9%). Prolongation of the growth to two months (instead of 25 days) resulted in increased content and diversity of very long-chain fatty acids including saturated species. The total very long-chain fatty acid content of 9.99% achieved in these experiments was 1.9–12.3-fold higher than in stress experiments. The highest variation was observed for oleic acid (3.4–33.7%). The novel strain showed the ability to accumulate lipids in amounts up to 639.8 mg L−1 under nitrogen and phosphorus starvation, which exceeds the previously obtained values for most Coelastrella strains. Thus, the newly identified MZ–Ch23 strain can be considered as a potential producer of omega-3 fatty acids on fully supplemented Bold’s basal medium or as a source of biomass with high content of saturated and monounsaturated fatty acids after nitrogen and phosphorus starvation.


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