An Evaluation of Lipid Profile and Antioxidant Activities of <i>Carica Papaya</i> Seed Oil in the Heart and Liver of Female Wistar Rats.

Objectives: Oxidative stress (OS)-related pathologic conditions in the tissues of living organism have been linked to exposure to stressful events within the environment. This study examined the impact of different kinds of stress exposure on glutathione (GSH)-related antioxidants. The effect of stress was examined using comparative levels of GSH, glutathione-S-transferase (GST) and glutathione peroxidase (GPx) in female Wistar rats. Materials and Methods: One hundred and sixty-eight adult female Wistar rats with body weights ranging between 150 and 200 g, were used for the study. The animals were distributed into 28 groups of six animals each. The experimental animals were routinely exposed to three different stressors; restraint chamber test, mirror chamber test and intruder chamber test for a duration of 1, 3 or 5 h per day for 1, 2 and 3 weeks, respectively. All animals were allowed free access to food (rat chaws), with water ad libitum. Animals were euthanise through cervical dislocation after the experimental period and the different target tissues were carefully harvested and homogenised for antioxidant estimation following standard procedure. Data obtained were statistically analysed and values expressed as mean ± standard error of mean and P < 0.05 level was considered as statistically significant. Results: Findings from this study elucidated the fact that exposure to stress is capable of causing marked OS and reducing GSH-based antioxidant activities in Wistar rats. A decline in the GSH level and GPx activity as observed in the study is an indication of alterations of kidney and brain tissue cellular integrity by free radicals generated during exposure to the stressors, while the observed significant increase in GST activity level in the affected tissues indicates compromised rapid exhaustion of the cellular system. Conclusion: Hence, we conclude that stress of different nature, intensity and duration can alter the levels of GSH-related antioxidants, especially in the kidney, ovary and brain tissues of stressed Wistar rats. The GSH levels in liver tissues were observed not to have changed significantly despite the oxidative damage caused by the stressors.

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The Interactive Effect of High Doses of Chromium(III) and Different Iron(III) Levels on the Carbohydrate Status, Lipid Profile, and Selected Biochemical Parameters in Female Wistar Rats

Nutrients ◽

10.3390/nu12103070 ◽

2020 ◽

Vol 12 (10) ◽

pp. 3070

Author(s):

Halina Zofia Staniek ◽

Ewelina Król ◽

Rafał Wojciech Wójciak

Keyword(s):

Insulin Resistance ◽

Lipid Profile ◽

Carbohydrate Metabolism ◽

Biochemical Parameters ◽

Wistar Rats ◽

Interactive Effect ◽

The Other ◽

Iron Deficient ◽

Female Wistar Rats ◽

High Doses

The aim of the study was to evaluate the main and interactive effects of chromium(III) propionate complex (Cr3) supplementation and different iron supply on the carbohydrate metabolism, lipid profile and other selected biochemical parameters of rats. The experiment was carried out in a two-factor design, in which rats were fed a diet with different proportions of Fe(III) and Cr(III) for six weeks. Fifty-four healthy female Wistar rats were divided into nine experimental groups with different Fe(III) levels, i.e. adequate—control group (45 mg/kg)—100% recommended daily dietary dose of Fe for rodents, deficient (5 mg/kg) and oversupply (180 mg/kg—400%). At the same time they were supplemented with Cr(III) of doses 1 (adequate), 50 and 500 mg/kg of diet. The activity and concentrations of most biochemical parameters were measured with standard enzymatic, kinetic, and colorimetric methods. HOMA-IR and QUICKI indexes were calculated according to appropriate formulas. It was found that there was an interactive effect of high Cr(III) doses and different Fe(III) levels in the diet on the carbohydrate metabolism and insulin resistance indexes. The presented results suggested that iron deficient diet fed animals led to insulin resistance; however, an effect is attenuated by Cr(III) supplementation at high doses. There were no significant changes in the rats’ lipid profile (except for the high density lipoprotein cholesterol (HDL-C) level) and most of the other biochemical parameters, such as the leptin, aspartate aminotransferase (AST), alanine transaminase (ALT), total protein (TP), creatinine (Crea) and the urea (BUN) concentrations. The study proved that the Cr(III) supplementation, independently and in combination with diversified Fe(III) content in the diet, affected the carbohydrate metabolism and insulin resistance indexes but did not affect lipid profile and most of the other biochemical parameters in healthy rats. The findings proved the role of Fe and Cr(III) and their interactions on disturbances carbohydrates metabolism.

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Cardiometabolic Changes in Different Gonadal Female States Caused by Mild Hyperuricemia and Exposure to a High-Fructose Diet

International Journal of Endocrinology ◽

10.1155/2018/6021259 ◽

2018 ◽

Vol 2018 ◽

pp. 1-9 ◽

Cited By ~ 1

Author(s):

J. Soutelo ◽

Y. A. Samaniego ◽

M. C. Fornari ◽

C. Reyes Toso ◽

O. J. Ponzo

Keyword(s):

Uric Acid ◽

Lipid Profile ◽

Wistar Rats ◽

Female Rats ◽

Treatment Control ◽

High Fructose Diet ◽

High Fructose ◽

Metabolic Systems ◽

Female Wistar Rats ◽

Oxonic Acid

Background. The objective of this study is to observe if mild hyperuricemia and a high-fructose diet influence the cardiovascular and metabolic systems in hypogonadic female Wistar rats compared to normogonadic female rats. Methods. Fifty-six (56) adult female Wistar rats were used in the present work. Animals were divided into two groups: normogonadic (NGN) and hypogonadic (HGN). These groups were also divided into four subgroups in accordance with the treatment: control with only water (C), fructose (F), oxonic acid (OA), and fructose + oxonic acid (FOA). Lipid profile, glycemia, uric acid, and creatinine determinations were assessed. Cardiovascular changes were evaluated by measuring blood pressure, myocyte volume, fibrosis, and intima-media aortic thickness. Results. HGN rats had higher levels of total cholesterol (TC) (p<0.01) and noHDLc (p<0.01), in addition to higher levels of uric acid (p<0.05). The OA group significantly increased myocyte volume (p<0.0001) and the percentage of fibrosis as well as the group receiving FOA (p<0.001) in both gonadal conditions, being greater in the HGN group. Hypogonadic animals presented a worse lipid profile. Conclusion. Mild hyperuricemia produces hypertension together with changes in the cardiac hypertrophy, fibrosis, and increased thickness of the intima media in hypogonadic rats fed high-fructose diet.

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Chia Seed Oil Ameliorates Doxorubicin-Induced Cardiotoxicity in Female Wistar Rats: An Electrocardiographic, Biochemical and Histopathological Approach

Cardiovascular Toxicology ◽

10.1007/s12012-021-09644-3 ◽

2021 ◽

Author(s):

Akheruz Zaman Ahmed ◽

Kamalesh D. Mumbrekar ◽

Shakta Mani Satyam ◽

Prakashchandra Shetty ◽

Melanie Rose D’Souza ◽

...

Keyword(s):

Wistar Rats ◽

Seed Oil ◽

Control Test ◽

Chia Seed ◽

Anti Cancer ◽

Before And After ◽

Female Wistar Rats ◽

Chia Seed Oil ◽

Pre Treatment ◽

Different Doses

AbstractDoxorubicin (DOX) is a potent anti-cancer antibiotic that was widely used for treatment of various cancers. It produces free radicals which result in extreme dose-limiting cardiotoxicity. This study investigated the cardioprotective potential of chia seed oil, an active polyphenolic nutraceutical against doxorubicin-induced cardiotoxicity in Wistar rats. Twenty-four female Wistar rats were divided into four groups (n = 6) which consist of normal control, DOX control, test-A and test-B group. Animals were prophylactically treated with two different doses of test drug, i.e. chia seed oil 2.5 ml/kg/day and 5 ml/kg/day in test-A and test-B groups orally for 7 days. Doxorubicin (25 mg/kg; single dose) was administered intraperitoneally to DOX control, Test-A and Test-B animals on the seventh day to induce cardiotoxicity. ECG analysis was done before and after treatment. Besides ECG, CK, CK-MB, LDH, AST, MDA and GSH were analyzed. DOX had significantly altered ECG, CK, CK-MB, LDH, AST, MDA and GSH. Pre-treatment with chia seed oil significantly alleviated DOX-induced ECG changes and also guarded against DOX-induced rise of serum CK, CK-MB and AST levels. Chia seed oil alleviated histopathological alteration in DOX-treated rats. It also significantly inhibited DOX-induced GSH depletion and elevation of MDA. The present study revealed that chia seed oil exerts cardioprotection against doxorubicin-induced cardiotoxicity in female Wistar rats. Our study opens the perspective to clinical studies to precisely consider chia seed oil as a potential chemoprotectant nutraceutical in the combination chemotherapy with doxorubicin to limit its cardiotoxicity.

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INVESTIGATION OF THE EFFECTS OF ALLOXAN-INDUCED DIABETES ON REPRODUCTIVE HORMONES (FOLLICLE STIMULATING, LUTEINIZING AND PROLACTIN), LIPID PROFILE AND SERUM ELECTROLYTES IN MALE AND FEMALE WISTAR RATS

African Journal of Science and Nature ◽

10.46881/ajsn.v7i0.155 ◽

2020 ◽

Vol 7 ◽

pp. 40

Author(s):

Bukonla Oluyemisi Adegbesan ◽

Olugbenga Owolabi Ogunlabi ◽

Temtope Abigail Fasanya ◽

Ayodeji Adebayo Adelegan ◽

Emmanuel Oladipupo Ajani

Keyword(s):

Lipid Profile ◽

Wistar Rats ◽

Reproductive Hormones ◽

Female Rats ◽

Male And Female ◽

Male And Female Rats ◽

Type1 Diabetes ◽

Female Wistar Rats ◽

The Impact ◽

Female Subjects

Human patients with Type1 diabetes and inadequate metabolic control have been found to exhibit a high prevalence of infertility but the exact mechanism by which this happens has not been fully elucidated. Alterations in the levels of lipids and electrolytes are considered to be good indications of some certain diseases including diabetes. This study aims at elucidating the impact of diabetes on the reproductive functions of male and female subjects as well as the responses of lipid profile which could be a determining factor affecting diabetic condition, to Alloxan-induced diabetes using male and female Wistar rats. To explore this, various hormonal assays assessing reproductive hormones such as Follicle stimulating hormone (FSH); Luteinizing hormone (LH); Prolactin hormone (PRL); lipid profile analyses and electrolytes measurements were employed. Our results showed that diabetes induce significant reduction in the levels of FSH and LH in both male and female rats (p<0.0001); significant reduction in the levels of LH in male (p<0.01) and female (p<0.0001); significant increase in the levels of prolactin (PRL) in male (p<0.05) and female (p<0.0001) ; significant increases in cholesterol levels in both male and female rats (p<0.01), an indication that diabetes is accompanied in most cases by hypercholesterolemia and triglycerides levels in both male (p<0.01) and female (p<0.0005) rats when compared to the control rats. The results obtained in this study shed more light on the mechanism by which fertility in male and female subjects is affected by diabetes and suggest possible mechanisms by which infertility could result from diabetes.

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Evaluation of the biochemical, haematological and histopathological parameters of female Wistar rats fed with aqueous and ethanol extracts of Aspilia africana leaves.

Journal of Herbmed Pharmacology ◽

10.34172/jhp.2020.33 ◽

2020 ◽

Vol 9 (3) ◽

pp. 257-267

Author(s):

Uche O. Arunsi ◽

Godwin C. Chinyere ◽

Kenneth O. Ngwogu ◽

Ada C. Ngwogu ◽

Okechukwu C. Atasie ◽

...

Keyword(s):

Acute Toxicity ◽

Lipid Profile ◽

Wistar Rats ◽

Lethal Dose ◽

White Blood Cells ◽

Saline Control ◽

Function Tests ◽

Female Wistar Rats ◽

Ethanol Extracts ◽

The Impact

Introduction: Aspilia africana is a plant commonly used to stop bleeding, heal wound, and manage various stomach complaints. This study aimed at evaluating the impact of aqueous and ethanol leaf extracts of A. africana on biochemical (liver function tests, renal function tests, and lipid profile), histopathological (kidney and liver) and haematological parameters of the female Wistar rats. Methods: To study acute toxicity, the median lethal dose (LD50) was determined by oral administration of different doses of the extract to 8 groups of 3 rats each and the animals were observed for 24 hours for signs of toxicity. To evaluate the toxicological effect of the extract, 3 groups of 5 animals each received 0.5 mL normal saline (control), 250 or 500 mg/kg of the extracts for 2 consecutive weeks. Results: Data revealed the LD50 of the extract to be >5000 mg/kg.bw. There was no significant variation in organosomatic indices of the animals fed with aqueous and ethanol extracts of A. africana leaves. In comparison with the control, there were significant increases (p<0.05) in serum liver and kidney biomarkers, high density lipoprotein, and white blood cells while some red cells indices, platelets, some lipid profile levels reduced significantly (p<0.05). A marked alteration in hepatic and renal architectures was also observed. Conclusion: The result of this study shows that the A. africana leaf may not be safe as medicine despite the outcome of LD50 acute toxicity studies. For it to be integrated in folk medicine, we recommend its use at minimal doses.

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EFFECTS OF RESVERATROL ON LIVER FUNCTION OF OBESE FEMALE WISTAR RATS

Ciência Animal Brasileira ◽

10.1590/1089-6891v17i332990 ◽

2016 ◽

Vol 17 (3) ◽

pp. 402-410 ◽

Cited By ~ 1

Author(s):

Nádia Araújo Miguel ◽

Silvia Franco Andrade ◽

Gisele Nai ◽

Cecília Braga Laposy ◽

Felipe Franco Nascimento ◽

...

Keyword(s):

Liver Function ◽

Lipid Profile ◽

Wistar Rats ◽

Liver Enzymes ◽

Obese Group ◽

Female Rats ◽

Control Group ◽

Obese Female ◽

Short Period ◽

Female Wistar Rats

Abstract Resveratrol has antioxidant, anti-inflammatory, lipolytic, and antifibrotic properties, which may be useful in supplementation of obese patients and with liver problems. This study evaluated the effects of 6-week resveratrol supplementation on the lipid profile and liver function of female Wistar rats fed a high-fat diet to induce obesity. Sixty-four Wistar rats were divided into 4 groups (n = 16): the control group (C); the control obese group (CO); the resveratrol group (R); and the resveratrol obese group (RO). At the end of the experiment, the animals were anesthetized for blood collection and subsequent euthanasia for collection of liver biopsy. The parameters for body weight, liver weight, retroperitoneal fat weight, serum lipid and liver profiles and histopathological analysis were evaluated. The 6-week resveratrol administration did not induce weight loss nor did it reduce the lipid profile; however, it decreased the liver enzymes aspartate aminotransferase (AST) and alkaline phosphatase (ALP) and reduced the incidence of steatosis (75.0%) in group RO compared with group CO (81.2%). Thus, we concluded that resveratrol supplementation for the short period of six weeks had a beneficial effect on liver function by reducing hepatic steatosis and the liver enzymes AST and ALP in obese female rats.

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