scholarly journals Determination of antioxidant activity, phenolic contents and antiviral potential of methanol extract of Euphorbia spinidens Bornm (Euphorbiaceae)

2016 ◽  
Vol 15 (4) ◽  
pp. 759 ◽  
Author(s):  
Ali Karimi ◽  
Marzieh Mohammadi-Kamalabadi ◽  
Mahmoud Rafieian-Kopaei ◽  
Leila Amjad ◽  
Loghman Salimzadeh
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Phenolic Contents

scholarly journals DETERMINATION OF THE ANTIOXIDANT ACTIVITY OF PRASMAN LEAF EXTRACTS (AYAPANA TRIPLINERVIS [VAHL]) AND THE TOTAL FLAVONOID AND PHENOL CONTENTS OF THE MOST ACTIVE EXTRACTS

2020 ◽  
pp. 107-111
Author(s):  
YUNITRIANTI ◽  
BERNA ELYA ◽  
ARIKADIA NOVIANI
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Total Flavonoid ◽  
Dpph Assay ◽  
Frap Assay ◽  
Methanol Extracts ◽  
Uv Visible ◽  
Antioxidant Effects

Objective: Prasman (Ayapana triplinervis [Vahl]) is a plant that can eliminate free radicals through its antioxidant effects. However, little research hasbeen conducted to explore the antioxidant activity of the plant.Methods: A. triplinervis (Vahl) leaves, which were determined by the Indonesian Institute of Sciences (LIPI) Bogor, were used in this study. Meanwhile,extraction was performed using n-hexane, ethyl acetate, and methanol as the solvents. Based on the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay usinga UV–visible spectrophotometer and based on ferric reducing antioxidant power (FRAP) assay using a microplate reader.Results: Based on the DPPH assay using a UV–visible spectrophotometer, n-hexane, ethyl acetate, and methanol extracts with a final concentrationof 25 μg/mL inhibited DPPH radical production by 38.91, 51.03, and 54.06%, respectively. Using the percent inhibition, the IC50 for the ethyl acetateand methanol extracts were 28.71 and 23.472 μg/mL, respectively. Based on FRAP assay using a microplate reader, the n-hexane, ethyl acetate, andmethanol extracts had ferrous equivalent antioxidant capacity values of 460, 828.99, and 940.22 μmol/g, respectively. The methanol extract had thegreatest antioxidant activity. The ethyl acetate and methanol extracts at the initial concentrations contained total phenol levels of 12.06 and 42.11 mgGAE/g extract, respectively, as well as total flavonoid levels of 3.24 and 3.41 mg QE/g extract, respectively.Conclusion: Based on the determination of phenol and flavonoid levels, the methanol extract had the greatest antioxidant effects.

scholarly journals CHEMICAL CONSTITUENTS, IN VITRO ANTIOXIDANT ACTIVITY, ORAL ACUTE TOXICITY AND LD50 DETERMINATION OF MORINGA OLEIFERA LEAVES

2017 ◽  
Vol 9 (5) ◽  
pp. 240
Author(s):  
Manal Mortady Hamed ◽  
Aboelfetoh Mohamed Abdalla ◽  
Mosad Ahmed Ghareeb ◽  
Said Abdelhalim Saleh
Keyword(s):  
Antioxidant Activity ◽  
Acute Toxicity ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Dry Extract ◽  
Phenolic Contents ◽  
Moringa Oleifera Leaves ◽  
Oral Acute Toxicity

Objective: The objective of this study was undertaken to estimate the total phenolic contents (TPCs), in vitro antioxidant of different solvent extracts of M. oleifera leaves, oral acute toxicity and LD50 determination of the 85% methanolic extract as well as the chromatographic isolation and identification of the extract constituents.Methods: The antioxidant activity of different solvent extracts of Moringa oleifera leaves were estimated using three antioxidant assays and the total phenolic contents (TPCs) were also evaluated using Folin-Ciocalteu’s assay. The n-BuOH extract undergoes further chromatographic isolation owing to the high antioxidant activity using 2, 2'-diphenyl-1-picrylhydrazyl radical (DPPH) method, which resulted in the isolation of seven compounds.Results: The results showed that the TPCs values of the tested extracts were varied from 309.52 to 43.28 mg gallic acid equivalent/g dry extract. The reducing power antioxidant activities (RPAA) were 0.434, 0.402, 0.395, 0.149, 0.143 and 0.124, while the total antioxidant capacity (TAC) values were 316.43, 203.35, 181.56, 86.70, 76.62 and 50.83 mg ascorbic acid equivalent/g dry extract; for n-BuOH, EtOAc, 85% MeOH, H2O, CH2Cl2, and pet. ether extracts, respectively. The oral acute toxicity study of the 85% methanol extracts of M. oleifera and M. peregrina revealed that; their LD50 values were 3458.3 and 4125 mg/kg respectively, thus the two plants could be classified as slightly toxic in the scale of Hodge and Sterner which reflected their nutrient values as edible plants. The isolated compounds were identified on the basis of their 1H and 13C-NMR spectra as; cis-p-coumaric acid 4-O-(2'-O-β-D-apiofuranosyl)-β-D-glucopyranoside (1), chlorogenic acid (2), niazirin (3), 3,4-dihydroxy-β-phenylethoxy-O-α-L-rhamnopyranosyl-(l→2)-α-L-rhamnopyranosyl-(1→3)-4-O-caffeoyl-β-D-glucopyranoside (4), gallic acid (5), taxifolin (6), and benzyl-carbamo-thioethionate (7).Conclusion: The M. oleifera leaves showed promising antioxidant activities and slightly toxic behavior.

scholarly journals ANTIFUNGAL POTENTIAL OF EXTRACTS AND FRACTIONS OF Randia nitida LEAVES ON SOYBEAN PATHOGENS AND THEIR PHYTOCHEMISTRY

2016 ◽  
Vol 29 (3) ◽  
pp. 594-602 ◽  
Author(s):  
STHEFANY CAROLINE BEZERRA DA CRUZ-SILVA ◽  
ROSEMARY MATIAS ◽  
JOSÉ ANTONIO MAIOR BONO ◽  
KAREN SILVA SANTOS ◽  
JULIANE LUDWIG
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Plant Pathogens ◽  
Methanol Extract ◽  
Chemical Constituents ◽  
Phytochemical Analysis ◽  
Total Phenols ◽  
Major Chemical ◽  
Crude Methanol Extract

ABSTRACT This study aimed to evaluate the antifungal activity of methanol extract and fractions of leaves of Randia nitida (Kunth) DC on Colletotrichum truncatum (Schw.) Andrus & Moore, Rhizoctonia solani Kühn and Sclerotinia sclerotiorum (Lib.) De Bary and perform chemical and antioxidant activity tests. Crude methanol extract (EBM) and fractions (hexane = FHX, dichloromethane = FDM and ethyl acetate = FAE) underwent classical phytochemical analysis and determination of total phenols, flavonoids and antioxidant activity. Samples were individually incorporated into PDA culture mediums (potato-dextrose-agar) at concentrations of 10, 20, 40, 80 and 160 µg mL-1 of extract or fraction. Mycelial growth was evaluated when control reached the edge of the plate. Phytochemical results indicated that flavonoids, steroids and triterpenoids are the major chemical constituents in both EBM and fractions. Total phenols, flavonoids and antioxidant activity were higher for FAE and EBM, followed by FDM and FHX; however, the latter showed higher content of steroids and triterpenoids compared to the other fractions. For C. truncatum, R. solani and S. sclerotiorum, the most efficient fractions were respectively FAE, FDM and FHX, all of them in the highest concentration (160 µg mL-1). The highest inhibition rate was observed against S. sclerotiorum, reaching 93.75%. We concluded that all fractions have antifungal activity on the plant pathogens, being FHX the most active against S. sclerotiorum, which might have been due to the presence of steroids and triterpenes.

scholarly journals Determination of antioxidant activity and total phenolic contents in yogurt added with black cumin (Nigella sativa L.) honey

2021 ◽  
Vol 32 (1) ◽  
pp. 1-5
Author(s):  
Özge Duygu Okur
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Nigella Sativa ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Black Cumin ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
High Level

Abstract This study aims to reveal the antioxidant activity and phenolic ingredients of yogurts added with black cumin (Nigella sativa L.) honey. Therefore, this study aimed to produce healthy food made with black cumin honey with increased total phenolic contents and antioxidant activity. The effect of varying degrees of black cumin honey addition (0, 2.5%, 5%, 10%, 15%) on the antioxidant activity and total phenolic contents of yogurt were examined for 28 days. The antioxidant activity was found between 14.33 and 17.41 mM TE. Total phenolic compounds were between 202.50 and 1415.00 mg GAE/kg. Some important differences were determined between yogurts with black cumin honey and the control with respect to phenolic contents during storage (p < 0.05). The results of study reveal that the augmentation of fortification rate increased the total phenolic characteristic and antioxidant activity of yogurt with black cumin honey. Significantly, these outcomes indicate that yogurt with black cumin honey have a high level of polyphenols and could be consumed as bioactive composition.

scholarly journals SCREENING AND DETERMINATION OF ANTIOXIDANT SCAVENGING ACTIVITY OF PIPER LONGUM AND EUCALYPTUS CAMALDULENSIS

2014 ◽  
Vol 1 (2) ◽  
pp. 80-84
Author(s):  
Kuppusamy Mohanraj ◽  
Naganathan Srinath
Keyword(s):  
Antioxidant Activity ◽  
Side Effects ◽  
Herbal Medicine ◽  
Methanol Extract ◽  
Eucalyptus Camaldulensis ◽  
Distilled Water ◽  
Scavenging Activity ◽  
Piper Longum ◽  
Eucalyptus Camaldulensis Dehnh

The herbal medicine is used from time immemorial to treat various common ailments like cold, cough and gastric problems. India has various system of treatment like Ayurveda, Siddha, Unani and Homeopathy which are devoid of side effects and treat the person with higher benefits. Piper longum fruitsand Eucalyptus camaldulensis Dehnh., leaves has been used as spices and for medicinal oil preparation for various treatment. We have made an attempt to elevate the importance of these herbal sources by understanding the antioxidant scavenging activity. We have extracted and processed the plants fruit andleaves using different solvents in 1:10 dilution such as petroleum ether, chloroform ethanol, hexane, methanol and distilled water. It was found that ethanol and methanol extract of Piper longum and Eucalyptus camaldulensis Dehnh., has higher antioxidant activity when compared with the standard Vitamin C and IC50value was observed to be nearly 70μg/ml for all the tests

scholarly journals Spectrophotometric Determination of Total Flavonoid Content in Biancaea Sappan (Caesalpinia sappan L.) Leaves

2021 ◽  
Vol 8 (3) ◽  
pp. 1-4
Author(s):  
Nurlinda Nurlinda ◽  
Virsa Handayani ◽  
Faradiba Abdul Rasyid
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Total Flavonoid ◽  
Total Flavonoids ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Caesalpinia Sappan ◽  
Antimicrobial Treatments

AbstractBiancaea sappan  (BS) is traditionally used to treat anticonvulsants, anti-inflammatory, antiproliferative, anticoagulant, antiviral, immunostimulant, antioxidant, and antimicrobial treatments. Flavonoids are found in Secang; flavonoids are secondary metabolites that have antioxidant activity. This study aims to identify the flavonoids using TLC and determination of flavonoids content in BS leaves. Initially, The methanol extract of BS was obtained by maceration with ethanol. The qualitative analysis of flavonoid was using TLC and visualization by sprayed with AlCl3. The determination of total flavonoid content is conducted based on the AlCl3 method with total flavonoids expressed in QE (Quercetin equivalent) at the maximum wavelength of 431 nm. The research results showed that BS leaves contain flavonoids and the total flavonoid content of BS leaf extract is 1.0318 mg QE / g extract.Keywords: Caesalpinia sappan; Flavonoid content, Spectrophotometric

scholarly journals Antioxidant and Antimalarial Activities of Phenolic Compounds from Leaves of Macaranga beccariana Merr.

2018 ◽  
Vol 15 (2) ◽  
pp. 106
Author(s):  
Eva Marliana ◽  
Chairul Saleh ◽  
Medi Hendra
Keyword(s):  
Antioxidant Activity ◽  
Structure Elucidation ◽  
Methanol Extract ◽  
Antimalarial Activity ◽  
Endemic Plant ◽  
1H And 13C Nmr ◽  
Antimalarial Agents ◽  
Falciparum Strain ◽  
Positive Controls

Macaranga beccariana Merr. is an endemic plant of Kalimantan which is no report yet about its biological activity and isolated compounds. Therefore, in order to know the potency of M. beccariana, determination of antioxidant and antimalarial activities along with isolation of bioactive compounds from this plant must be carried out. In this research, the leaves of M. beccariana was extracted using methanol to obtain methanol extract. Furthermore, the extract was separated and purified to obtain compound 1 and compound 2. Based on structure elucidation using spectrometer analysis including UV, 1H and 13C NMR, compounds 1 and 2 which belong to phenolics were identified as 4-hidroxybenzoic acid and 3,4-dihidroxybenzoic acid, respectively. Moreover, antioxidant activity using DPPH (2,2-diphenyl-1-picrylhydrazyl) and antimalarial activity towards Plasmodium falciparum strain 3D7 using Giemsa staining were performed. Rutine and chloroquine difosfat were used as positive controls for antioxidant and antimalarial, respectively. The results showed that the methanol extract of M. beccariana and its isolated compounds (1 and 2) are active for those activities. It can be concluded that the leaves of M. beccariana has good potency as antioxidant and antimalarial agents.

scholarly journals Determination of phenolic compounds and evaluation of antioxidant capacity of Campomanesia adamantium leaves

2008 ◽  
Vol 33 (4) ◽  
pp. 53-60 ◽  
Author(s):  
I. D. Coutinho ◽  
R. G. Coelho ◽  
V. M. F. Kataoka ◽  
N. K. Honda ◽  
J. R. M. Silva ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Radical Scavenging ◽  
Acid System ◽  
Mato Grosso ◽  
Phenolic Contents ◽  
Wide Range ◽  
High Antioxidant Activity ◽  
Mato Grosso Do Sul

Five flavanones and three chalcones were isolated from Campomanesia adamantium Berg. (Myrtaceae) leaves. The contents of these compounds were determined by HPLC. The phenolic contents were also determined. The monitoring of the antioxidant activity was carried out by inhibition of peroxidation using the linoleic acid system and radical-scavenging (DPPH). The plants were collected from 4 distinct cities of the Mato Grosso do Sul State, Brazil. The different samples exhibited a range of 4.67-232.35 mg/g chalcones and 15.62-50.71 mg/g flavanones and phenolic contents of the 7.24-21.19 mg/g gallic acid. All extracts showed high antioxidant activity with a wide range of the radical-scavenging (DPPH) from 52.0 to 92.2 % and inhibition oxidation of linoleic acid from 14.6 to 94.2%.

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