Sulfanilamide benzotriazole tetrazole inhibits neuronal apoptosis in neonatal rats by targeting JNK and p38 MAPK pathways

Purpose: To investigate the neuroprotective effect of sulfanilamide benzotriazole tetrazole (SBT) in neonatal rats exposed to isoflurane, and also to elucidate the underlying mechanism. Methods: Rat pups (n = 60) were randomly assigned to six groups of 10 pups each: normal control group, negative control group, 5 mg/kg SBT group, 10 mg/kg SBT group, 15 mg/kg SBT group, and 20 mg/kg SBT group. With exception of normal control group, pups were exposed to isoflurane (0.75 %) for 6 h on postnatal day 7. The negative control group was not treated, while pups in the four treatment groups received 5, 10, 15 and 20 mg/kg SBT, respectively, 1 h after exposure to anaesthesia. TUNEL assay was used to determine the extent of apoptosis in cornu ammonis area-1 (CA-1), cornu ammonis area-3 (CA-3) and dentate gyrus of rat hippocampal tissues. Expressions of apoptotic and anti-apoptotic proteins were determined using Western blotting. Evaluation of learning and memorizing ability was done using Morris water maze test. Results: Isoflurane significantly increased the extent of apoptosis in CA-1, CA-3 and dentate gyrus of rat hippocampal tissues (p < 0.05). However, treatment with SBT significantly and dose-dependently reduced neuronal apoptosis (p < 0.05). The expression of caspase 3 was significantly upregulated by isoflurane, but was significantly and dose-dependently down-regulated by SBT (p < 0.05). Isoflurane significantly increased Bax expression, and decreased the expression of bcl-2 (p < 0.05). The effects of isoflurane on the expression of these proteins were significantly and dose-dependently reversed by SBT (p < 0.05). The expression of bcl xL in rat hippocampal tissues was significantly down-regulated by isoflurane, but was significantly and dose-dependently upregulated by SBT (p < 0.05). The escape latency of pups was significantly higher in negative control group than in normal control group, but SBT treatment significantly and dose-dependently reversed this trend (p < 0.05). Conclusion: These results suggest that SBT prevents neuronal apoptosis, and improves the ability to learn and memorize in neonatal rats exposed to isoflurane via regulation of apoptotic, JNK and p38 MAPK protein expressions.

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The Effect of Different Extracts of Beetroots as Antioxidant and Anti-Anaemia On Phenylhydrazine-Induced Rats

European Pharmaceutical Journal ◽

10.2478/afpuc-2020-0014 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Ni Putu Ermi Hikmawanti ◽

Lusi Putri Dwita ◽

Dimas W. Wisnunanda ◽

Fanny Farista

Keyword(s):

Antioxidant Activity ◽

Normal Control ◽

Antioxidant Activities ◽

Negative Control Group ◽

Negative Control ◽

Pharmaceutical Product ◽

Normal Control Group ◽

Male Rats ◽

Control Group ◽

Hydroethanolic Extract

Abstract Aim evaluate antioxidant and anti-anaemia activity of dichloromethane, hydroethanolic, and alkaloids-free hydroethanolic extracts of beetroot (Beta vulgaris (L.) subsp. vulgaris) on phenylhydrazine-induced rats. Methods Male rats were divided into five groups: normal control group, negative control group, dichloromethane extract group, hydroethanolic extract group, and alkaloids-free hydroethanolic extract group. All groups were induced with phenylhydrazine (30 mg.Kg−1 BW) for three days, except for the normal control group. After induction, each treatment group received each extract (200 mg.Kg−1 BW) for 21 days. The haematology parameters (haemoglobin levels, the number of erythrocytes, and haematocrit levels) were measured using Haematology Analyzer, and the antioxidant activity was measured through MDA level parameters in rats. Data were analysed using one-way ANOVA and then continued with the Tukey test. Results The results showed that the hydroethanolic extract of beetroot increased the percentage of erythrocytes (33.5%), haemoglobin (25%), and haematocrit (24.4%) to the negative control group, which was comparable to the normal control group (p > 0.05). In addition, the best antioxidant activity was shown in the hydroethanolic extract of beetroot, which is comparable to the normal group (p > 0.05). Conclusion The beetroot hydroethanolic crude extract could be potentially produced in a natural pharmaceutical product as a beneficial resource within anti-anaemia and antioxidant activities.

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STRUKTUR HISTOLOGI HATI TIKUS PUTIH (Rattus norvegicus Berkenhout 1769) DENGAN PEMBERIAN RAMUAN TRADISIONAL MASYARAKAT MELAYU LINGGA, KEPULAUAN RIAU

EKOTONIA Jurnal Penelitian Biologi Botani Zoologi dan Mikrobiologi ◽

10.33019/ekotonia.v3i1.753 ◽

2019 ◽

Vol 3 (1) ◽

pp. 11-19

Author(s):

Fitmawati Fitmawati ◽

Titrawani Titrawani ◽

Welly Safitri

Keyword(s):

Normal Control ◽

Rattus Norvegicus ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Normal Control Group ◽

Control Group ◽

Different Types ◽

Histopathologic Changes ◽

Accessible Category

Lingga Island is the center of the Malay kingdom of Kepulauan Riau Province with local wisdom that is still ingrained in the community. Obat pahit is a decoction of linguistic stew of ethnic lingga community which is believed to be a youthful remedy and maintain stamina. This study aims to find histopathologic changes of liver white mice after the decoction of herb concoction of Obat pahit. The percentage of intercellular damage was nonexistent from the normal control group and positive control but different from the negative control group. Different types of medicinal herbs with different dosage levels. But the damage is still in the normal, accessible category that has no toxic effects from bitter herbs on liver organ.

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Pengaruh Pemberian Cuka Apel 'A' terhadap Kadar MDA Hepar Tikus Wistar Jantan yang Diinduksi Parasetamol Dosis Toksik

Pustaka Kesehatan ◽

10.19184/pk.v6i2.7667 ◽

2018 ◽

Vol 6 (2) ◽

pp. 272

Author(s):

Niki Niki Rahmawati ◽

Sugiyanta Sugiyanta ◽

Elly Nurus Sakinah

Keyword(s):

Treatment Group ◽

Normal Control ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

High Dose ◽

Toxic Dose ◽

Group A ◽

Post Hoc ◽

Cytochrome P 450

High dose of paracetamol is metabolized by cytochrome P-450 become free radical N-acetyl-p-benzoquinoneimine (NAPQI) but liver Glutathione (GSH) is not adequate to change it become nonreactive metabolite so that NAPQI bind to unsaturated fatty acid of cell membrane, causing lipid peroxidation and increase liver Malondialdehyde (MDA). 'A' apple vinegar contains anthocyanin with an antioxidant effect by electron donor to NAPQI and acetic acid to improve liver GSH level. The aim of research was to investigate the effect of 'A' apple vinegar on the rat liver MDA induced by toxic dose of paracetamol. Research groups consist of normal control (CMC Na 1% 1 ml for 14 days), negative control (CMC Na 1% 1ml for 14 days + paracetamol 291.6 mg/200gBW on the day 12nd,13rd,14th), and treatment group ('A' apple vinegar 0.4 ml/150gBW for 14 days + paracetamol 291.6 mg/200gBW on the day 12nd,13rd,14th). Liver MDA was measured on the day 15th with competitive ELISA. The average of normal control group was 21.58 ng/ml, negative control group was 70,71 ng/ml, treatment group was 37,67 ng/ml. One way ANOVA and Post hoc LSD test showed significantly differences between all groups (p<0,05). It can be concluded that 'A' apple vinegar had an effect on the liver MDA induced by toxic dose of paracetamol. Keywords: Paracetamol, NAPQI, MDA, 'A' apple vinegar, antioxidant

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Some Haematological and Biochemical Investigations on Duck Virus Hepatitis following Administration of Glycyrrhizin

ISRN Pharmacology ◽

10.1155/2013/849412 ◽

2013 ◽

Vol 2013 ◽

pp. 1-10 ◽

Cited By ~ 5

Author(s):

Faten A. Okda ◽

Safaa Yassein ◽

Alaa R. Ahmed ◽

Hamdy Soufy ◽

Soad M. Nasr

Keyword(s):

Normal Control ◽

Hepatitis Virus ◽

Hypochromic Anemia ◽

Negative Control ◽

Normal Control Group ◽

Licorice Root ◽

Control Group ◽

Duck Hepatitis Virus ◽

Duck Hepatitis ◽

Group 2

The present study aimed to investigate the protective effect of glycyrrhizin (locally isolated and purified from licorice root) against duck hepatitis virus through the assessment of some hematological and biochemical parameters. One hundred and sixty white Pekin ducklings—one day old—were randomly divided into four equal groups. Group (1) was kept as normal control. Group (2) was inoculated I/P with 10 mg glycyrrhizin/kg BW, three times per week for four weeks. Group (3) was inoculated I/M with 0.5 ml of live attenuated DHV vaccine. Group (4) was inoculated with both glycyrrhizin (10 mg/kg BW I/P, three times per week for four weeks) and live attenuated DHV vaccine (0.5 ml, I/M). Then, all groups of treatment were challenged using virulent DHV except for 20 ducklings from the normal control group which were continued to be kept as negative control. The results revealed that duck hepatitis virus (DHV) caused macrocytic hypochromic anemia, leukopenia, hypoproteinemia, hypoalbuminemia, hyperglycemia, hypercholesterolemia, and marked elevation of liver enzymes and renal parameters. In conclusion, glycyrrhizin injected alone or in combination with DHV vaccine protected or ameliorated the deteriorating effects induced by DHV vaccine and/or duck hepatitis virus infection by improvement of erythrogram and leukogram, as well as liver and kidney functions.

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Effects of Powdered Stem Bark of Terminalia avicennioides Made as Dietary Feed Fed to Mice Infected with Plasmodium berghei, on Liver Function

Asian Journal of Biochemistry, Genetics and Molecular Biology ◽

10.9734/ajbgmb/2019/v2i430066 ◽

2019 ◽

pp. 1-11

Author(s):

Afolabi Owoloye ◽

Olusegun Matthew Akanbi ◽

Oluwafemi Shittu Bakare

Keyword(s):

Liver Function ◽

Normal Control ◽

Plasmodium Berghei ◽

Negative Control Group ◽

Stem Bark ◽

Positive Control ◽

Negative Control ◽

Antiplasmodial Activity ◽

Control Group ◽

Significant Difference

Aim: This study aimed to investigate the antiplasmodial activity and effect of stem bark of Terminalia avicennioides made as dietary feed fed to mice infected with Plasmodium berghei, on some serum biochemistry. Methodology: Twenty (20) mice were divided into four groups. Group 1 was not infected with Plasmodium berghei (normal control), Group 2 was infected with P. berghei but not treated (negative control). Group 3 was infected and treated with 5.0 mg/kg of Arthemeter-Lumefantrine (positive control). Groups 4 was infected and fed with treated feed (T. avicennioides). Treatments were carried out for five days. Blood was taken daily from the tail of the mice before treatment for the assessment of parasitaemia. The animals were sacrificed on the fifth day and the whole blood was collected into EDTA bottle. Serum obtained was used to assay for biochemical parameters. Results: Parasitaemia count was significantly lower (p<0.05) in all the treated groups when compared with the negative control group. The high-density lipoprotein was significantly higher (P<0.05) in the normal control (123.14±3.19) when compared with the positive control (99.18±2.76), negative control (85.29±0.85) and the group treated with T avicennioides (86.14±3.21). The serum alanine aminotransferase, alkaline phosphatase and aspartate aminotransferase level in the group treated with T. avicennioides (167.90±4.13, 15.87±1.32 and 17.50±1.95) respectively were significantly reduced (p<0.05) when compared with the negative control (197.25±5.44, 20.01±1.32 and 26.71±0.45) respectively. The mean bilirubin and albumin level in the negative control showed no significant difference when compared with the group fed with T. avicennioides. Conclusion: The study concluded that T. avicennioides have antiplasmodial activity with a mild adverse effect on liver function.

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Potential of Cocoa Ethanol Extract Toward Nematocyst Tubul Firing Inhibition of Physalia Utriculus Toxin In Vitro

Journal of Agromedicine and Medical Sciences ◽

10.19184/ams.v5i2.9623 ◽

2019 ◽

Vol 5 (2) ◽

pp. 30

Author(s):

Sarwendah Siswi Winasis ◽

Al Munawir ◽

Adelia Handoko

Keyword(s):

Normal Control ◽

Ethanol Extract ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Control Group Design ◽

Average Percentage ◽

Post Test

There was estimated 150 million envenomation cases due to jellyfish stings occur globally every year. 100 from 10,000 jellyfish species in the world known to be dangerous, one them is Physalia utriculus. The aim of this study was to determine the potential of cocoa (Theobroma cacao L.) ethanol extract toward nematocyst tubul firing inhibition of jellyfish (Physalia utriculus) toxin in vitro. The method was true experimental design with post test only control group design. The study divided into 8 groups: 1 normal control, 1 positive control, 1 negative control, and 5 treatments grups by giving cocoa ethanol extract with concentration 20%, 2%, 0.2%, 0.02%, 0.002%. The observation was made by calculating the percentage number of firing nematocysts. The result showed average percentage of firing nematocyst in the normal control group was 42.50 ± 3.18, positive control group was 37.97 ± 5.57, negative control group was 52.44 ± 2.98, and treatment group which given with cocoa ethanol extract 20%, 2%, 0.2%, 0.02%, 0.002% were 48.24 ± 5.37; 40.62 ± 7.10; 29.45 ± 5.39; 37.60 ± 9.78; 41.11 ± 3.92, respectively. The One Way Annova statistical results test show significance value 0.001 (p≤0.05). The conclusion of this study was the cocoa ethanol extract with concentration 0.2% has most potential to inhibit the jellyfish (P. utriculus) nematocyst tubule firing in vitro. Keywords: Physalia utriculus, cocoa, toxin, jellyfish

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Effect of HylocereusPolyrhizus Rind Extract Toward Interleukin-1β, Vascular Endothelial Growth Factor Expression, Endometriosis Implant Area

International Journal of Pharmaceutical and Clinical Research ◽

10.25258/ijpcr.v9i08.9588 ◽

2017 ◽

Vol 9 (08) ◽

Author(s):

YanuarEka P. ◽

Hendy Hendarto ◽

Widjiati .

Keyword(s):

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Treatment Group ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Vascular Endothelial ◽

Mice Model ◽

Endothelial Growth Factor ◽

Fruit Rind

Retrograde menstruation lead to I Kappa B Kinase (IKK) fosforilation in peritoneum macrophage and cause secretion of proinflammatory cytokine interleukin1β then stimulate endometriosis cell to produce Vascular Endothelial Growth Factor which lead to increasing of endometriosis lession seen as endometriosis implant area. Cytokine secretion was inhibited through prevention of NF-κB activation by dragon red fruit rind extract (Hylocereuspolyrhizus). The aim of this reserach is to know the effect of dragon red fuit rind extract with 0,25; 0,5; and 1 mg/g bodyweight dosage toward IL-1β, VEGF expression and implant area in endometriosis mice model. The design of this experiment was randomized post test only control group design.Endometrios mice model were made in 14 days and split into two group, positive control group and treatment group after two week negative control group and postive control group were given Na-CMC 0,5% solution consequetively, and treatment group were given dragon red fruit extract with different dosage. Signification number for IL-1β is p>0,05, signification number for VEGF is p>0,05, and implant area signification number is p>0,05. Administration of dragon red fruit rind extract can decrease IL-1β, VEGF, and implant area.

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The Comparison of Ostecyte in the Pressure area and Tension area on Tooth Movement Because of Hyperbaric Oxygen Therapy

DENTA ◽

10.30649/denta.v12i1.162 ◽

2018 ◽

Vol 12 (1) ◽

pp. 34

Author(s):

Arya Barahmanta ◽

Muhammad Faizal Winaris ◽

Pambudi Raharjo

Keyword(s):

Hyperbaric Oxygen ◽

Oxygen Therapy ◽

Tooth Movement ◽

Bone Remodelling ◽

Orthodontic Tooth Movement ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Pressure Area

Background: Orthodontic tooth movement is a interaction prosess of resorption and deposition of bone remodeling. Orthodontic tooth movement by mechanical strength causes changes in alveolar bone. Osteocyte is an essential cell to respond bone remodelling. Hyperbaric Oxygen Therapy affects production of osteocyte because it can release Reactive Oxygen Species (ROS) and Nitrid Oxide (NO). Purpose: To determine the difference number of osteocyte in pressure and tension area during tooth movement by adjuvant of Hyperbaric Oxygen 2,4 ATA during 7 days starting on day 8 to day 14. Materials and Methods: This research used Completery Randomized Control Group Post Test Only Design. 36 cavia cobaya (male) were divided into 3 groups randomly : the negative control groups, positive control group, and treatment group. Preparat staining used Hematoxylin Eosin (HE) and calculated on microscop 1000x with 20 field of view. Data analyses used one way ANOVA and LSD test then compared each area by using paired T test. Result: The data showed that the treatment group (P=10,67) tension area has the highest number of osteocyte than negative control group (K-=3,67), positive control (K+=7,42). In the pressure area showed that negative control group (K-=5,00) has the highest than positive control group (K+=3,83) and treatment (P=3,25). Conclusion: Therapy HBO 2,4 ATA 7 days starting on day 8 to day 14 is could increase osteocyte in the tissue to stimulate process of bone remodelling.<pre> </pre>Keywords: Hyperbaric Oxygen, Tooth movement, Bone remodeling, Osteocyte Correspondence: Arya Brahmanta, Department of Orthodonty, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Phone 031-5945864, Email: <a href="mailto:[email protected]">arya.brahmanta@hangtuah.ac.id</a>

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Antiurolithiatic effect of Cymbopogon Proximus, Alhagi Maurorum, on Sulfadimidine induced urolithiasis in male New Zealand rabbits

Mansoura Veterinary Medical Journal ◽

10.35943/mvmj.2019.01.107 ◽

2019 ◽

Vol 20 (1) ◽

pp. 12-18

Author(s):

Sameh El-Nabtity

Keyword(s):

New Zealand ◽

Intramuscular Injection ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Aqueous Extracts ◽

Blood And Urine Samples ◽

Group 2 ◽

New Zealand Rabbits ◽

Group 1

The present study aimed to investigate the prophylactic effect of Cymbopogon proximus and Alhagi maurorum on Sulfadimidine induced urolithiasis in rabbits . Thirty New Zealand male rabbits were allocated into six equal groups (each of five): Group (1) was used as a negative control. Group(2) were administered sulfadimidine (200mg/kg) by intramuscular injection.Groups(3) and (4) were administered sulfadimidine(200mg/kg) by intramuscular injection and 330mg/kg of Cymbopogon proximus alcoholic and aqueous extracts respectively orally.Groups(5) and (6) were administered sulfadimidine(200mg/kg) by intramuscular injection and 400mg/kg of Alhagi maurorum alcoholic and aqueous extracts respectively orally. The period of experiment was 10 days. Blood and urine samples were collected from rabbits on the 10th day. The results recorded a significant decrease in serum creatinine, urea, uric acid and crystalluria in Cymbopogon proximus and Alhagi maurorum groups compared to sulfadimidine treated group.We conclude that Cymbopogon proximus and Alhagi maurorum have a nephroprotective and antiurolithiatic effects against sulfadimidine induced crystalluria.

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